SSGCID: Target Page

MyavA.00358.n

Probable enoyl-CoA hydratase

CENTER ID:	MyavA.00358.n
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00358.n.A1.GE28531	Full length( MyavA.00358.n )		1	267

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.4990
RefSeq:	YP_884204.1
UniProt:	A0A0H2ZV41

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTSLDAPVL REVDADGVAR VRLNRPQTSN GLNVETLKAL HDAVLTCHAD PTVKVVLLSG AGRNFCAGGD VHTFESKGAD LPDYLREATA WLQLATAALI QLRAPVVTAV QGFAAGGGGL GLVCASDVVL AGRSAKFFSG AVRVGMAPDG GSSVTLTQLV GLRQALRILL TNPTLGAEEA AAIGLITDVV DDDALTEEAE RIAKTLAAMP PKALSATKRL VWSGVGVPVE SRLAEEARIV SELSGTADAL EGLRAVIERR PPRFTGA

NT Sequence

ATGACCACCA GTCTGGACGC ACCGGTACTT CGCGAGGTCG ACGCCGACGG CGTCGCCCGC GTCCGGCTCA ACCGCCCCCA GACGTCGAAC GGGCTCAACG TCGAAACCCT GAAGGCGCTG CACGACGCGG TACTGACCTG CCACGCCGAC CCGACCGTCA AAGTCGTCCT GCTCTCCGGT GCGGGGCGTA ACTTCTGCGC GGGTGGCGAC GTGCACACGT TCGAGTCCAA GGGTGCGGAT TTGCCGGATT ACCTGCGGGA GGCTACGGCA TGGCTGCAGT TGGCCACCGC CGCCTTGATT CAGCTGCGGG CCCCGGTCGT CACCGCCGTG CAAGGGTTCG CCGCCGGCGG TGGTGGCCTG GGCCTGGTCT GCGCGTCCGA CGTCGTCCTC GCCGGCCGTT CGGCGAAGTT CTTCTCGGGT GCGGTGCGTG TGGGCATGGC CCCGGACGGG GGCTCTTCGG TGACCTTGAC ACAGCTGGTC GGCCTGCGGC AGGCCCTGAG AATTTTATTG ACCAACCCGA CGCTGGGAGC CGAGGAAGCC GCTGCGATTG GGTTGATCAC TGACGTCGTC GACGACGACG CGCTCACCGA GGAGGCTGAG CGGATCGCCA AGACACTCGC CGCGATGCCG CCGAAGGCCC TGTCGGCCAC CAAGCGGCTG GTGTGGTCGG GCGTGGGCGT TCCCGTCGAG TCACGTCTGG CCGAGGAAGC TCGTATTGTC TCCGAATTGT CCGGCACCGC CGATGCTCTG GAGGGGCTTC GCGCGGTGAT CGAGCGGCGA CCCCCGAGGT TCACCGGCGC A

Details for MyavA.00358.n.A1.GE28531

HARVESTED ON:	2/9/2010
SEQUENCED ON:	2/22/2010
EXPECTED MW:	29kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTTSLDAPV LREVDADGVA RVRLNRPQTS NGLNVETLKA LHDAVLTCHA DPTVKVVLLS GAGRNFCAGG DVHTFESKGA DLPDYLREAT AWLQLATAAL IQLRAPVVTA VQGFAAGGGG LGLVCASDVV LAGRSAKFFS GAVRVGMAPD GGSSVTLTQL VGLRQALRIL LTNPTLGAEE AAAIGLITDV VDDDALTEEA ERIAKTLAAM PPKALSATKR LVWSGVGVPV ESRLAEEARI VSELSGTADA LEGLRAVIER RPPRFTGA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca ccagtctgga cgcaccggta cttcgcgagg tcgacgccga cggcgtcgcc cgcgtccggc tcaaccgccc ccagacgtcg aacgggctca acgtcgaaac cctgaaggcg ctgcacgacg cggtactgac ctgccacgcc gacccgaccg tcaaagtcgt cctgctctcc ggtgcggggc gtaacttctg cgcgggtggc gacgtgcaca cgttcgagtc caagggtgcg gatttgccgg attacctgcg ggaggctacg gcatggctgc agttggccac cgccgccttg attcagctgc gggccccggt cgtcaccgcc gtgcaagggt tcgccgccgg cggtggtggc ctgggcctgg tctgcgcgtc cgacgtcgtc ctcgccggcc gttcggcgaa gttcttctcg ggtgcggtgc gtgtgggcat ggccccggac gggggctctt cggtgacctt gacacagctg gtcggcctgc ggcaggccct gagaatttta ttgaccaacc cgacgctggg agccgaggaa gccgctgcga ttgggttgat cactgacgtc gtcgacgacg acgcgctcac cgaggaggct gagcggatcg ccaagacact cgccgcgatg ccgccgaagg ccctgtcggc caccaagcgg ctggtgtggt cgggcgtggg cgttcccgtc gagtcacgtc tggccgagga agctcgtatt gtctccgaat tgtccggcac cgccgatgct ctggaggggc ttcgcgcggt gatcgagcgg cgacccccga ggttcaccgg cgcataataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaccagt ctggacgcac cggtacttcg cgaggtcgac gccgacggcg tcgcccgcgt ccggctcaac cgcccccaga cgtcgaacgg gctcaacgtc gaaaccctga aggcgctgca cgacgcggta ctgacctgcc acgccgaccc gaccgtcaaa gtcgtcctgc tctccggtgc ggggcgtaac ttctgcgcgg gtggcgacgt gcacacgttc gagtccaagg gtgcggattt gccggattac ctgcgggagg ctacggcatg gctgcagttg gccaccgccg ccttgattca gctgcgggcc ccggtcgtca ccgccgtgca agggttcgcc gccggcggtg gtggcctggg cctggtctgc gcgtccgacg tcgtcctcgc cggccgttcg gcgaagttct tctcgggtgc ggtgcgtgtg ggcatggccc cggacggggg ctcttcggtg accttgacac agctggtcgg cctgcggcag gccctgagaa ttttattgac caacccgacg ctgggagccg aggaagccgc tgcgattggg ttgatcactg acgtcgtcga cgacgacgcg ctcaccgagg aggctgagcg gatcgccaag acactcgccg cgatgccgcc gaaggccctg tcggccacca agcggctggt gtggtcgggc gtgggcgttc ccgtcgagtc acgtctggcc gaggaagctc gtattgtctc cgaattgtcc ggcaccgccg atgctctgga ggggcttcgc gcggtgatcg agcggcgacc cccgaggttc accggcgcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a