MysmA.00358.c

Enoyl-CoA hydratase

CENTER ID: MysmA.00358.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.c.A1.GE28614 Full length( MysmA.00358.c ) 1 252
MysmA.00358.c.B1.GE43080 Full length( MysmA.00358.c ) 1 252
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00358.c.A1.PS00564 Full length( MysmA.00358.c ) 1 252

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4003
RefSeq: YP_888360.1
UniProt: A0QZM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGEPGSVEV ARDGDVAVVT LRREHKLNAL SMHMETQLLT ALRTQEVRTS RAVVVTGGNT VFSAGADVNE IRTLTPDAIL AYYRNSGSVY EVFAELPQPT VVAVAGYCLG GGLELSLAGD IRIADETAVF GFPEIQIGIL PSSGGVTRAA RTLGYGRATD LILRGRRFGA HQALQWGLLT EVTNKGSHIR TALTIAHEIA EHDPLVVSVT KQVIHASPDA SQAASLLLER LAYAVLNRTT PAAHAHDEVK QP
NT Sequence
GTGAACGGCG AACCCGGATC GGTTGAGGTC GCCCGCGACG GTGACGTCGC CGTCGTCACC CTGCGCCGTG AACACAAACT CAACGCCTTG TCGATGCACA TGGAAACCCA ACTGCTCACA GCGTTGCGAA CGCAGGAAGT CCGAACCAGC CGCGCCGTTG TGGTCACCGG CGGGAACACC GTGTTCAGCG CAGGCGCGGA CGTCAACGAA ATCCGCACCC TCACACCGGA TGCCATCCTT GCCTACTACC GGAACTCCGG TTCGGTCTAT GAGGTGTTCG CCGAGCTCCC TCAACCCACC GTGGTCGCCG TAGCGGGTTA CTGCCTCGGC GGCGGTCTAG AGCTCAGCCT GGCCGGAGAC ATCCGGATCG CCGACGAAAC GGCCGTCTTC GGCTTTCCCG AAATCCAGAT AGGCATCCTG CCTTCCTCGG GCGGCGTCAC CCGGGCGGCG CGCACGCTCG GATACGGCCG GGCCACCGAC CTGATCCTGC GTGGACGCCG ATTCGGCGCC CACCAGGCCC TGCAATGGGG TCTGCTCACC GAGGTCACCA ACAAGGGCAG CCACATCCGA ACAGCACTGA CCATCGCCCA TGAGATAGCC GAGCACGACC CGCTCGTCGT CTCGGTGACC AAACAGGTCA TCCACGCCAG TCCCGATGCC TCCCAGGCAG CCTCGCTACT CCTCGAGCGA CTGGCCTACG CCGTCCTCAA CAGAACCACG CCCGCCGCCC ACGCCCACGA CGAAGTGAAA CAACCA
Details for MysmA.00358.c.A1.GE28614
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 29kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MGTLEAQTQG PGSMVNGEPG SVEVARDGDV AVVTLRREHK LNALSMHMET QLLTALRTQE VRTSRAVVVT GGNTVFSAGA DVNEIRTLTP DAILAYYRNS GSVYEVFAEL PQPTVVAVAG YCLGGGLELS LAGDIRIADE TAVFGFPEIQ IGILPSSGGV TRAARTLGYG RATDLILRGR RFGAHQALQW GLLTEVTNKG SHIRTALTIA HEIAEHDPLV VSVTKQVIHA SPDASQAASL LLERLAYAVL NRTTPAAHAH DEV
Validated NT Sequence
ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggtgaacgg cgaacccgga tcggttgagg tcgcccgcga cggtgacgtc gccgtcgtca ccctgcgccg tgaacacaaa ctcaacgcct tgtcgatgca catggaaacc caactgctca cagcgttgcg aacgcaggaa gtccgaacca gccgcgccgt tgtggtcacc ggcgggaaca ccgtgttcag cgcaggcgcg gacgtcaacg aaatccgcac cctcacaccg gatgccatcc ttgcctacta ccggaactcc ggttcggtct atgaggtgtt cgccgagctc cctcaaccca ccgtggtcgc cgtagcgggt tactgcctcg gcggcggtct agagctcagc ctggccggag acatccggat cgccgacgaa acggccgtct tcggctttcc cgaaatccag ataggcatcc tgccttcctc gggcggcgtc acccgggcgg cgcgcacgct cggatacggc cgggccaccg acctgatcct gcgtggacgc cgattcggcg cccaccaggc cctgcaatgg ggtctgctca ccgaggtcac caacaagggc agccacatcc gaacagcact gaccatcgcc catgagatag ccgagcacga cccgctcgtc gtctcggtga ccaaacaggt catccacgcc agtcccgatg cctcccaggc agcctcgcta ctcctcgagc gactggccta cgccgtcctc aacagaacca cgcccgccgc ccacgcccac gacgaagtga aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNGEPGSV EVARDGDVAV VTLRREHKLN ALSMHMETQL LTALRTQEVR TSRAVVVTGG NTVFSAGADV NEIRTLTPDA ILAYYRNSGS VYEVFAELPQ PTVVAVAGYC LGGGLELSLA GDIRIADETA VFGFPEIQIG ILPSSGGVTR AARTLGYGRA TDLILRGRRF GAHQALQWGL LTEVTNKGSH IRTALTIAHE IAEHDPLVVS VTKQVIHASP DASQAASLLL ERLAYAVLNR TTPAAHAHDE VKQP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacggc gaacccggat cggttgaggt cgcccgcgac ggtgacgtcg ccgtcgtcac cctgcgccgt gaacacaaac tcaacgcctt gtcgatgcac atggaaaccc aactgctcac agcgttgcga acgcaggaag tccgaaccag ccgcgccgtt gtggtcaccg gcgggaacac cgtgttcagc gcaggcgcgg acgtcaacga aatccgcacc ctcacaccgg atgccatcct tgcctactac cggaactccg gttcggtcta tgaggtgttc gccgagctcc ctcaacccac cgtggtcgcc gtagcgggtt actgcctcgg cggcggtcta gagctcagcc tggccggaga catccggatc gccgacgaaa cggccgtctt cggctttccc gaaatccaga taggcatcct gccttcctcg ggcggcgtca cccgggcggc gcgcacgctc ggatacggcc gggccaccga cctgatcctg cgtggacgcc gattcggcgc ccaccaggcc ctgcaatggg gtctgctcac cgaggtcacc aacaagggca gccacatccg aacagcactg accatcgccc atgagatagc cgagcacgac ccgctcgtcg tctcggtgac caaacaggtc atccacgcca gtcccgatgc ctcccaggca gcctcgctac tcctcgagcg actggcctac gccgtcctca acagaaccac gcccgccgcc cacgcccacg acgaagtgaa acaaccaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MysmA.00358.c.B1.GE43080
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV NGEPGSVEVA RDGDVAVVTL RREHKLNALS MHMETQLLTA LRTQEVRTSR AVVVTGGNTV FSAGADVNEI RTLTPDAILA YYRNSGSVYE VFAELPQPTV VAVAGYCLGG GLELSLAGDI RIADETAVFG FPEIQIGILP SSGGVTRAAR TLGYGRATDL ILRGRRFGAH QALQWGLLTE VTNKGSHIRT ALTIAHEIAE HDPLVVSVTK QVIHASPDAS QAASLLLERL AYAVLNRTTP AAHXXDEVKQ P
Validated NT Sequence
atcttactca cttatggttg tttcacttcg tcnnnnncgt gggcggcggg cgtggttctg ttgaggacgg cgtaggccag tcgctcgagg agtagcgagg ctgcctggga ggcatcggga ctggcgtgga tgacctgttt ggtcaccgag acgacgagcg ggtcgtgctc ggctatctca tgggcgatgg tcagtgctgt tcggatgtgg ctgcccttgt tggtgacctc ggtgagcaga ccccattgca gggcctggtg ggcgccgaat cggcgtccac gcaggatcag gtcggtggcc cggccgtatc cgagcgtgcg cgccgcccgg gtgacgccgc ccgaggaagg caggatgcct atctggattt cgggaaagcc gaagacggcc gtttcgtcgg cgatccggat gtctccggcc aggctgagct ctagaccgcc gccgaggcag taacccgcta cggcgaccac ggtgggttga gggagctcgg cgaacacctc atagaccgaa ccggagttcc ggtagtaggc aaggatggca tccggtgtga gggtgcggat ttcgttgacg tccgcgcctg cgctgaacac ggtgttcccg ccggtgacca caacggcgcg gctggttcgg acttcctgcg ttcgcaacgc tgtgagcagt tgggtttcca tgtgcatcga caaggcgttg agtttgtgtt cacggcgcag ggtgacgacg gcgacgtcac cgtcgcgggc gacctcaacc gatccgggtt cgccgttcac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMN GEPGSVEVAR DGDVAVVTLR REHKLNALSM HMETQLLTAL RTQEVRTSRA VVVTGGNTVF SAGADVNEIR TLTPDAILAY YRNSGSVYEV FAELPQPTVV AVAGYCLGGG LELSLAGDIR IADETAVFGF PEIQIGILPS SGGVTRAART LGYGRATDLI LRGRRFGAHQ ALQWGLLTEV TNKGSHIRTA LTIAHEIAEH DPLVVSVTKQ VIHASPDASQ AASLLLERLA YAVLNRTTPA AHAHDEVKQP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaacg gcgaacccgg atcggttgag gtcgcccgcg acggtgacgt cgccgtcgtc accctgcgcc gtgaacacaa actcaacgcc ttgtcgatgc acatggaaac ccaactgctc acagcgttgc gaacgcagga agtccgaacc agccgcgccg ttgtggtcac cggcgggaac accgtgttca gcgcaggcgc ggacgtcaac gaaatccgca ccctcacacc ggatgccatc cttgcctact accggaactc cggttcggtc tatgaggtgt tcgccgagct ccctcaaccc accgtggtcg ccgtagcggg ttactgcctc ggcggcggtc tagagctcag cctggccgga gacatccgga tcgccgacga aacggccgtc ttcggctttc ccgaaatcca gataggcatc ctgccttcct cgggcggcgt cacccgggcg gcgcgcacgc tcggatacgg ccgggccacc gacctgatcc tgcgtggacg ccgattcggc gcccaccagg ccctgcaatg gggtctgctc accgaggtca ccaacaaggg cagccacatc cgaacagcac tgaccatcgc ccatgagata gccgagcacg acccgctcgt cgtctcggtg accaaacagg tcatccacgc cagtcccgat gcctcccagg cagcctcgct actcctcgag cgactggcct acgccgtcct caacagaacc acgcccgccg cccacgccca cgacgaagtg aaacaaccat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for MysmA.00358.c.A1.PS00564
PURIFICATION DATe: 4/20/2010
CONCENTRATION: 37.05mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVNGEPG SVEVARDGDV AVVTLRREHK LNALSMHMET QLLTALRTQE VRTSRAVVVT GGNTVFSAGA DVNEIRTLTP DAILAYYRNS GSVYEVFAEL PQPTVVAVAG YCLGGGLELS LAGDIRIADE TAVFGFPEIQ IGILPSSGGV TRAARTLGYG RATDLILRGR RFGAHQALQW GLLTEVTNKG SHIRTALTIA HEIAEHDPLV VSVTKQVIHA SPDASQAASL LLERLAYAVL NRTTPAAHAH DEV
Validated NT Sequence
ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggtgaacgg cgaacccgga tcggttgagg tcgcccgcga cggtgacgtc gccgtcgtca ccctgcgccg tgaacacaaa ctcaacgcct tgtcgatgca catggaaacc caactgctca cagcgttgcg aacgcaggaa gtccgaacca gccgcgccgt tgtggtcacc ggcgggaaca ccgtgttcag cgcaggcgcg gacgtcaacg aaatccgcac cctcacaccg gatgccatcc ttgcctacta ccggaactcc ggttcggtct atgaggtgtt cgccgagctc cctcaaccca ccgtggtcgc cgtagcgggt tactgcctcg gcggcggtct agagctcagc ctggccggag acatccggat cgccgacgaa acggccgtct tcggctttcc cgaaatccag ataggcatcc tgccttcctc gggcggcgtc acccgggcgg cgcgcacgct cggatacggc cgggccaccg acctgatcct gcgtggacgc cgattcggcg cccaccaggc cctgcaatgg ggtctgctca ccgaggtcac caacaagggc agccacatcc gaacagcact gaccatcgcc catgagatag ccgagcacga cccgctcgtc gtctcggtga ccaaacaggt catccacgcc agtcccgatg cctcccaggc agcctcgcta ctcctcgagc gactggccta cgccgtcctc aacagaacca cgcccgccgc ccacgcccac gacgaagtga aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNGEPGSV EVARDGDVAV VTLRREHKLN ALSMHMETQL LTALRTQEVR TSRAVVVTGG NTVFSAGADV NEIRTLTPDA ILAYYRNSGS VYEVFAELPQ PTVVAVAGYC LGGGLELSLA GDIRIADETA VFGFPEIQIG ILPSSGGVTR AARTLGYGRA TDLILRGRRF GAHQALQWGL LTEVTNKGSH IRTALTIAHE IAEHDPLVVS VTKQVIHASP DASQAASLLL ERLAYAVLNR TTPAAHAHDE VKQP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAACGGC GAACCCGGAT CGGTTGAGGT CGCCCGCGAC GGTGACGTCG CCGTCGTCAC CCTGCGCCGT GAACACAAAC TCAACGCCTT GTCGATGCAC ATGGAAACCC AACTGCTCAC AGCGTTGCGA ACGCAGGAAG TCCGAACCAG CCGCGCCGTT GTGGTCACCG GCGGGAACAC CGTGTTCAGC GCAGGCGCGG ACGTCAACGA AATCCGCACC CTCACACCGG ATGCCATCCT TGCCTACTAC CGGAACTCCG GTTCGGTCTA TGAGGTGTTC GCCGAGCTCC CTCAACCCAC CGTGGTCGCC GTAGCGGGTT ACTGCCTCGG CGGCGGTCTA GAGCTCAGCC TGGCCGGAGA CATCCGGATC GCCGACGAAA CGGCCGTCTT CGGCTTTCCC GAAATCCAGA TAGGCATCCT GCCTTCCTCG GGCGGCGTCA CCCGGGCGGC GCGCACGCTC GGATACGGCC GGGCCACCGA CCTGATCCTG CGTGGACGCC GATTCGGCGC CCACCAGGCC CTGCAATGGG GTCTGCTCAC CGAGGTCACC AACAAGGGCA GCCACATCCG AACAGCACTG ACCATCGCCC ATGAGATAGC CGAGCACGAC CCGCTCGTCG TCTCGGTGAC CAAACAGGTC ATCCACGCCA GTCCCGATGC CTCCCAGGCA GCCTCGCTAC TCCTCGAGCG ACTGGCCTAC GCCGTCCTCA ACAGAACCAC GCCCGCCGCC CACGCCCACG ACGAAGTGAA ACAACCAAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA