MymaA.00358.c
Enoyl-CoA hydratase, EchA8_6
| CENTER ID: | MymaA.00358.c |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00358.c.A1.GE29997 | Full length( MymaA.00358.c ) | 1 | 252 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00358.c.A1.PS00818 | Full length( MymaA.00358.c ) | 1 | 252 | ||
| MymaA.00358.c.A1.PS00819 | Full length( MymaA.00358.c ) | 1 | 252 |
Structures
3QMJ
DEPOSITED: 2/4/2011
DETERMINATION: XRay
CLONE: MymaA.00358.c.A1.GE29997
PROTEIN: MymaA.00358.c.A1.PS00818
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129422 |
| PATRIC ID: | fig|216594.6.peg.2951 |
| RefSeq: | YP_001851056.1 |
| UniProt: | B2HD64 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLQIDDDNR VRTLTLNRPE ALNAFNEALY DATAQALLDA ADDPQVAVVL LTGSGRGFSA GTDLAEMQAR ITDPNFSEGK FGFRGLIKAL AGFPKPLICA VNGLGVGIGA TILGYADLAF MSSTARLKCP FTSLGVAPEA ASSYLLPQLV GRQNAAWLLM SSEWIDAEEA LRMGLVWRIC SPEELLPEAR RHAEILAAKP ISSLMAVKHT MVEPNRAQIA AASARENAHF AELMGAQANA AALADFTDRR RP
NT Sequence
GTGACGCTGC AGATCGATGA TGACAACCGC GTACGCACGC TCACGCTGAA CCGTCCCGAG GCGCTCAACG CATTTAACGA AGCCCTCTAC GACGCGACCG CCCAGGCCCT CTTGGACGCG GCCGACGATC CGCAGGTTGC CGTGGTCTTG CTGACCGGCT CGGGTCGCGG CTTCAGCGCT GGCACCGATC TTGCCGAGAT GCAGGCTCGC ATCACCGATC CAAATTTCTC CGAGGGGAAA TTTGGCTTCC GCGGTCTCAT CAAAGCACTC GCCGGGTTCC CAAAGCCCCT GATTTGCGCC GTCAACGGGC TTGGCGTCGG GATCGGCGCC ACCATTCTGG GCTATGCCGA TCTGGCTTTC ATGTCCTCGA CAGCGCGCCT GAAGTGCCCC TTCACCAGCC TCGGCGTGGC TCCCGAAGCG GCCTCGTCCT ATCTTCTGCC GCAACTGGTG GGCCGCCAGA ACGCGGCTTG GTTGCTGATG TCCTCGGAAT GGATTGACGC CGAAGAGGCA CTGCGGATGG GCCTGGTCTG GAGAATCTGC AGTCCTGAGG AACTGCTACC AGAGGCGCGT CGTCATGCCG AAATACTTGC TGCCAAGCCG ATTTCCAGCT TGATGGCGGT CAAGCACACG ATGGTCGAAC CCAACCGTGC CCAGATCGCG GCGGCCAGCG CACGAGAGAA CGCGCACTTC GCGGAGCTGA TGGGCGCTCA AGCCAACGCC GCTGCCCTTG CCGATTTCAC CGATCGCCGG CGACCG
Details for MymaA.00358.c.A1.GE29997
| HARVESTED ON: | 7/13/2010 |
| SEQUENCED ON: | 8/18/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 29kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTLQIDDD NRVRTLTLNR PEALNAFNEA LYDATAQALL DAADDPQVAV VLLTGSGRGF SAGTDLAEMQ ARITDPNFSE GKFGFRGLIK ALAGFPKPLI CAVNGLGVGI GATILGYADL AFMSSTARLK CPFTSLGVAP EAASSYLLPQ LVGRQNAAWL LMSSEWIDAE EALRMGLVWR ICSPEELLPE ARRHAEILAA KPISSLMAVK HTMVEPNRAQ IAAASARENA HFAELMGAQA NAAALADFTD RRR
Validated NT Sequence
ttattatcgc cggcgatcgg tgaaatcggc aagggcagcg gcgttggctt gagcgcccat cagctccgcg aagtgcgcgt tctctcgtgc gctggccgcc gcgatctggg cacggttggg ttcgaccatc gtgtgcttga ccgccatcaa gctggaaatc ggcttggcag caagtatttc ggcatgacga cgcgcctctg gtagcagttc ctcaggactg cagattctcc agaccaggcc catccgcagt gcctcttcgg cgtcaatcca ttccgaggac atcagcaacc aagccgcgtt ctggcggccc accagttgcg gcagaagata ggacgaggcc gcttcgggag ccacgccgag gctggtgaag gggcacttca ggcgcgctgt cgaggacatg aaagccagat cggcatagcc cagaatggtg gcgccgatcc cgacgccaag cccgttgacg gcgcaaatca ggggctttgg gaacccggcg agtgctttga tgagaccgcg gaagccaaat ttcccctcgg agaaatttgg atcggtgatg cgagcctgca tctcggcaag atcggtgcca gcgctgaagc cgcgacccga gccggtcagc aagaccacgg caacctgcgg atcgtcggcc gcgtccaaga gggcctgggc ggtcgcgtcg tagagggctt cgttaaatgc gttgagcgcc tcgggacggt tcagcgtgag cgtgcgtacg cggttgtcat catcgatctg cagcgtcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggtan ntctccttct t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTLQIDDD NRVRTLTLNR PEALNAFNEA LYDATAQALL DAADDPQVAV VLLTGSGRGF SAGTDLAEMQ ARITDPNFSE GKFGFRGLIK ALAGFPKPLI CAVNGLGVGI GATILGYADL AFMSSTARLK CPFTSLGVAP EAASSYLLPQ LVGRQNAAWL LMSSEWIDAE EALRMGLVWR ICSPEELLPE ARRHAEILAA KPISSLMAVK HTMVEPNRAQ IAAASARENA HFAELMGAQA NAAALADFTD RRR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgctg cagatcgatg atgacaaccg cgtacgcacg ctcacgctga accgtcccga ggcgctcaac gcatttaacg aagccctcta cgacgcgacc gcccaggccc tcttggacgc ggccgacgat ccgcaggttg ccgtggtctt gctgaccggc tcgggtcgcg gcttcagcgc tggcaccgat cttgccgaga tgcaggctcg catcaccgat ccaaatttct ccgaggggaa atttggcttc cgcggtctca tcaaagcact cgccgggttc ccaaagcccc tgatttgcgc cgtcaacggg cttggcgtcg ggatcggcgc caccattctg ggctatgccg atctggcttt catgtcctcg acagcgcgcc tgaagtgccc cttcaccagc ctcggcgtgg ctcccgaagc ggcctcgtcc tatcttctgc cgcaactggt gggccgccag aacgcggctt ggttgctgat gtcctcggaa tggattgacg ccgaagaggc actgcggatg ggcctggtct ggagaatctg cagtcctgag gaactgctac cagaggcgcg tcgtcatgcc gaaatacttg ctgccaagcc gatttccagc ttgatggcgg tcaagcacac gatggtcgaa cccaaccgtg cccagatcgc ggcggccagc gcacgagaga acgcgcactt cgcggagctg atgggcgctc aagccaacgc cgctgccctt gccgatttca ccgatcgccg gcgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MymaA.00358.c.A1.PS00818
| PURIFICATION DATe: | 10/28/2010 |
| CONCENTRATION: | 69.82mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTLQIDDD NRVRTLTLNR PEALNAFNEA LYDATAQALL DAADDPQVAV VLLTGSGRGF SAGTDLAEMQ ARITDPNFSE GKFGFRGLIK ALAGFPKPLI CAVNGLGVGI GATILGYADL AFMSSTARLK CPFTSLGVAP EAASSYLLPQ LVGRQNAAWL LMSSEWIDAE EALRMGLVWR ICSPEELLPE ARRHAEILAA KPISSLMAVK HTMVEPNRAQ IAAASARENA HFAELMGAQA NAAALADFTD RRR
Validated NT Sequence
ttattatcgc cggcgatcgg tgaaatcggc aagggcagcg gcgttggctt gagcgcccat cagctccgcg aagtgcgcgt tctctcgtgc gctggccgcc gcgatctggg cacggttggg ttcgaccatc gtgtgcttga ccgccatcaa gctggaaatc ggcttggcag caagtatttc ggcatgacga cgcgcctctg gtagcagttc ctcaggactg cagattctcc agaccaggcc catccgcagt gcctcttcgg cgtcaatcca ttccgaggac atcagcaacc aagccgcgtt ctggcggccc accagttgcg gcagaagata ggacgaggcc gcttcgggag ccacgccgag gctggtgaag gggcacttca ggcgcgctgt cgaggacatg aaagccagat cggcatagcc cagaatggtg gcgccgatcc cgacgccaag cccgttgacg gcgcaaatca ggggctttgg gaacccggcg agtgctttga tgagaccgcg gaagccaaat ttcccctcgg agaaatttgg atcggtgatg cgagcctgca tctcggcaag atcggtgcca gcgctgaagc cgcgacccga gccggtcagc aagaccacgg caacctgcgg atcgtcggcc gcgtccaaga gggcctgggc ggtcgcgtcg tagagggctt cgttaaatgc gttgagcgcc tcgggacggt tcagcgtgag cgtgcgtacg cggttgtcat catcgatctg cagcgtcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggtan ntctccttct t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTLQIDDD NRVRTLTLNR PEALNAFNEA LYDATAQALL DAADDPQVAV VLLTGSGRGF SAGTDLAEMQ ARITDPNFSE GKFGFRGLIK ALAGFPKPLI CAVNGLGVGI GATILGYADL AFMSSTARLK CPFTSLGVAP EAASSYLLPQ LVGRQNAAWL LMSSEWIDAE EALRMGLVWR ICSPEELLPE ARRHAEILAA KPISSLMAVK HTMVEPNRAQ IAAASARENA HFAELMGAQA NAAALADFTD RRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGCTG CAGATCGATG ATGACAACCG CGTACGCACG CTCACGCTGA ACCGTCCCGA GGCGCTCAAC GCATTTAACG AAGCCCTCTA CGACGCGACC GCCCAGGCCC TCTTGGACGC GGCCGACGAT CCGCAGGTTG CCGTGGTCTT GCTGACCGGC TCGGGTCGCG GCTTCAGCGC TGGCACCGAT CTTGCCGAGA TGCAGGCTCG CATCACCGAT CCAAATTTCT CCGAGGGGAA ATTTGGCTTC CGCGGTCTCA TCAAAGCACT CGCCGGGTTC CCAAAGCCCC TGATTTGCGC CGTCAACGGG CTTGGCGTCG GGATCGGCGC CACCATTCTG GGCTATGCCG ATCTGGCTTT CATGTCCTCG ACAGCGCGCC TGAAGTGCCC CTTCACCAGC CTCGGCGTGG CTCCCGAAGC GGCCTCGTCC TATCTTCTGC CGCAACTGGT GGGCCGCCAG AACGCGGCTT GGTTGCTGAT GTCCTCGGAA TGGATTGACG CCGAAGAGGC ACTGCGGATG GGCCTGGTCT GGAGAATCTG CAGTCCTGAG GAACTGCTAC CAGAGGCGCG TCGTCATGCC GAAATACTTG CTGCCAAGCC GATTTCCAGC TTGATGGCGG TCAAGCACAC GATGGTCGAA CCCAACCGTG CCCAGATCGC GGCGGCCAGC GCACGAGAGA ACGCGCACTT CGCGGAGCTG ATGGGCGCTC AAGCCAACGC CGCTGCCCTT GCCGATTTCA CCGATCGCCG GCGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA
Details for MymaA.00358.c.A1.PS00819
| PURIFICATION DATe: | 10/28/2010 |
| CONCENTRATION: | 72.46mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTLQIDDD NRVRTLTLNR PEALNAFNEA LYDATAQALL DAADDPQVAV VLLTGSGRGF SAGTDLAEMQ ARITDPNFSE GKFGFRGLIK ALAGFPKPLI CAVNGLGVGI GATILGYADL AFMSSTARLK CPFTSLGVAP EAASSYLLPQ LVGRQNAAWL LMSSEWIDAE EALRMGLVWR ICSPEELLPE ARRHAEILAA KPISSLMAVK HTMVEPNRAQ IAAASARENA HFAELMGAQA NAAALADFTD RRR
Validated NT Sequence
ttattatcgc cggcgatcgg tgaaatcggc aagggcagcg gcgttggctt gagcgcccat cagctccgcg aagtgcgcgt tctctcgtgc gctggccgcc gcgatctggg cacggttggg ttcgaccatc gtgtgcttga ccgccatcaa gctggaaatc ggcttggcag caagtatttc ggcatgacga cgcgcctctg gtagcagttc ctcaggactg cagattctcc agaccaggcc catccgcagt gcctcttcgg cgtcaatcca ttccgaggac atcagcaacc aagccgcgtt ctggcggccc accagttgcg gcagaagata ggacgaggcc gcttcgggag ccacgccgag gctggtgaag gggcacttca ggcgcgctgt cgaggacatg aaagccagat cggcatagcc cagaatggtg gcgccgatcc cgacgccaag cccgttgacg gcgcaaatca ggggctttgg gaacccggcg agtgctttga tgagaccgcg gaagccaaat ttcccctcgg agaaatttgg atcggtgatg cgagcctgca tctcggcaag atcggtgcca gcgctgaagc cgcgacccga gccggtcagc aagaccacgg caacctgcgg atcgtcggcc gcgtccaaga gggcctgggc ggtcgcgtcg tagagggctt cgttaaatgc gttgagcgcc tcgggacggt tcagcgtgag cgtgcgtacg cggttgtcat catcgatctg cagcgtcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggtan ntctccttct t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTLQIDDD NRVRTLTLNR PEALNAFNEA LYDATAQALL DAADDPQVAV VLLTGSGRGF SAGTDLAEMQ ARITDPNFSE GKFGFRGLIK ALAGFPKPLI CAVNGLGVGI GATILGYADL AFMSSTARLK CPFTSLGVAP EAASSYLLPQ LVGRQNAAWL LMSSEWIDAE EALRMGLVWR ICSPEELLPE ARRHAEILAA KPISSLMAVK HTMVEPNRAQ IAAASARENA HFAELMGAQA NAAALADFTD RRR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGCTG CAGATCGATG ATGACAACCG CGTACGCACG CTCACGCTGA ACCGTCCCGA GGCGCTCAAC GCATTTAACG AAGCCCTCTA CGACGCGACC GCCCAGGCCC TCTTGGACGC GGCCGACGAT CCGCAGGTTG CCGTGGTCTT GCTGACCGGC TCGGGTCGCG GCTTCAGCGC TGGCACCGAT CTTGCCGAGA TGCAGGCTCG CATCACCGAT CCAAATTTCT CCGAGGGGAA ATTTGGCTTC CGCGGTCTCA TCAAAGCACT CGCCGGGTTC CCAAAGCCCC TGATTTGCGC CGTCAACGGG CTTGGCGTCG GGATCGGCGC CACCATTCTG GGCTATGCCG ATCTGGCTTT CATGTCCTCG ACAGCGCGCC TGAAGTGCCC CTTCACCAGC CTCGGCGTGG CTCCCGAAGC GGCCTCGTCC TATCTTCTGC CGCAACTGGT GGGCCGCCAG AACGCGGCTT GGTTGCTGAT GTCCTCGGAA TGGATTGACG CCGAAGAGGC ACTGCGGATG GGCCTGGTCT GGAGAATCTG CAGTCCTGAG GAACTGCTAC CAGAGGCGCG TCGTCATGCC GAAATACTTG CTGCCAAGCC GATTTCCAGC TTGATGGCGG TCAAGCACAC GATGGTCGAA CCCAACCGTG CCCAGATCGC GGCGGCCAGC GCACGAGAGA ACGCGCACTT CGCGGAGCTG ATGGGCGCTC AAGCCAACGC CGCTGCCCTT GCCGATTTCA CCGATCGCCG GCGAAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA