MyavA.00358.f

Enoyl-CoA hydratase

CENTER ID: MyavA.00358.f
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyavA.00358.f.A1.GE28523 Full length( MyavA.00358.f ) 1 274
MyavA.00358.f.AE1.GE43229 Full length( MyavA.00358.f ) 1 274
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyavA.00358.f.A1.PS00580 Full length( MyavA.00358.f ) 1 274

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.1552
RefSeq: YP_880824.1
UniProt: A0A0H3A2H0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAADHVVTD EAVLYQTTET GVAVVTFNRP ERLNAWGPDI SSGFYACIDR AEADPAVRVI VLTGAGRGFC AGANLGGAGS IDAKLGDGDT DVTALVGDRH PHFLTALRKP IIAAINGACV GIGLTHALMC DVRFAAAGAK FATAFTRRGL IAEYGITWIL PRLAGWGAAM DLLLSGRTFF AEEAAKLGLV NEIVAPDELM ARALTYADDI ARNCSPASLA VIKRQAYDDA LRDVVDVSAR AETLMHESLQ RPDVIEGITS FLEKRPPRFP PLAT
NT Sequence
ATGAGTGCCG CCGACCATGT GGTCACCGAC GAGGCGGTGC TGTACCAGAC CACGGAAACC GGCGTTGCGG TCGTGACGTT CAACCGGCCC GAGCGGCTCA ACGCCTGGGG ACCCGACATC TCGAGCGGCT TCTACGCGTG CATCGACCGC GCCGAGGCGG ACCCCGCGGT CCGGGTGATC GTGTTGACGG GCGCCGGTCG GGGGTTCTGC GCGGGCGCCA ACCTGGGCGG CGCGGGATCC ATCGATGCGA AGCTGGGCGA CGGCGACACC GACGTCACCG CGTTGGTCGG CGATCGCCAT CCCCACTTCC TGACCGCACT GCGCAAACCG ATCATCGCCG CGATCAACGG CGCCTGCGTC GGCATCGGGC TGACCCACGC CCTGATGTGC GACGTGCGGT TCGCGGCCGC CGGCGCCAAG TTCGCCACCG CGTTCACCCG CCGGGGACTG ATCGCCGAGT ACGGCATCAC CTGGATCCTG CCGCGGCTGG CCGGCTGGGG CGCGGCGATG GACCTGCTGT TGAGCGGGCG CACCTTCTTC GCCGAGGAGG CCGCAAAACT CGGGCTGGTC AACGAGATCG TCGCACCCGA CGAGCTGATG GCCCGCGCCC TGACCTACGC CGACGACATC GCCCGCAACT GTTCGCCGGC GTCGCTGGCG GTGATCAAGC GGCAGGCCTA CGACGACGCG CTGCGCGACG TCGTCGACGT CAGCGCCCGC GCCGAAACCC TCATGCACGA GTCGCTGCAG CGCCCCGACG TCATCGAGGG CATCACCAGC TTCCTGGAGA AGCGGCCGCC GCGGTTCCCA CCCCTGGCCA CC
Details for MyavA.00358.f.A1.GE28523
HARVESTED ON: 2/9/2010
SEQUENCED ON: 2/22/2010
EXPECTED MW: 31kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMSAADHV VTDEAVLYQT TETGVAVVTF NRPERLNAWG PDISSGFYAC IDRAEADPAV RVIVLTGAGR GFCAGANLGG AGSIDAKLGD GDTDVTALVG DRHPHFLTAL RKPIIAAING ACVGIGLTHA LMCDVRFAAA GAKFATAFTR RGLIAEYGIT WILPRLAGWG AAMDLLLSGR TFFAEEAAKL GLVNEIVAPD ELMARALTYA DDIARNCSPA SLAVIKRQAY DDALRDVVDV SARAETLMHE SLQRPDVIEG ITSFLEKRPP RFPPLAT
Validated NT Sequence
gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgagtgccg ccgaccatgt ggtcaccgac gaggcggtgc tgtaccagac cacggaaacc ggcgttgcgg tcgtgacgtt caaccggccc gagcggctca acgcctgggg acccgacatc tcgagcggct tctacgcgtg catcgaccgc gccgaggcgg accccgcggt ccgggtgatc gtgttgacgg gcgccggtcg ggggttctgc gcgggcgcca acctgggcgg cgcgggatcc atcgatgcga agctgggcga cggcgacacc gacgtcaccg cgttggtcgg cgatcgccat ccccacttcc tgaccgcact gcgcaaaccg atcatcgccg cgatcaacgg cgcctgcgtc ggcatcgggc tgacccacgc cctgatgtgc gacgtgcggt tcgcggccgc cggcgccaag ttcgccaccg cgttcacccg ccggggactg atcgccgagt acggcatcac ctggatcctg ccgcggctgg ccggctgggg cgcggcgatg gacctgctgt tgagcgggcg caccttcttc gccgaggagg ccgcaaaact cgggctggtc aacgagatcg tcgcacccga cgagctgatg gcccgcgccc tgacctacgc cgacgacatc gcccgcaact gttcgccggc gtcgctggcg gtgatcaagc ggcaggccta cgacgacgcg ctgcgcgacg tcgtcgacgt cagcgcccgc gccgaaaccc tcatgcacga gtcgctgcag cgccccgacg tcatcgaggg catcaccagc ttcctggaga agcggccgcc gcggttccca cccctggcca cctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAADHVVT DEAVLYQTTE TGVAVVTFNR PERLNAWGPD ISSGFYACID RAEADPAVRV IVLTGAGRGF CAGANLGGAG SIDAKLGDGD TDVTALVGDR HPHFLTALRK PIIAAINGAC VGIGLTHALM CDVRFAAAGA KFATAFTRRG LIAEYGITWI LPRLAGWGAA MDLLLSGRTF FAEEAAKLGL VNEIVAPDEL MARALTYADD IARNCSPASL AVIKRQAYDD ALRDVVDVSA RAETLMHESL QRPDVIEGIT SFLEKRPPRF PPLAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgccgcc gaccatgtgg tcaccgacga ggcggtgctg taccagacca cggaaaccgg cgttgcggtc gtgacgttca accggcccga gcggctcaac gcctggggac ccgacatctc gagcggcttc tacgcgtgca tcgaccgcgc cgaggcggac cccgcggtcc gggtgatcgt gttgacgggc gccggtcggg ggttctgcgc gggcgccaac ctgggcggcg cgggatccat cgatgcgaag ctgggcgacg gcgacaccga cgtcaccgcg ttggtcggcg atcgccatcc ccacttcctg accgcactgc gcaaaccgat catcgccgcg atcaacggcg cctgcgtcgg catcgggctg acccacgccc tgatgtgcga cgtgcggttc gcggccgccg gcgccaagtt cgccaccgcg ttcacccgcc ggggactgat cgccgagtac ggcatcacct ggatcctgcc gcggctggcc ggctggggcg cggcgatgga cctgctgttg agcgggcgca ccttcttcgc cgaggaggcc gcaaaactcg ggctggtcaa cgagatcgtc gcacccgacg agctgatggc ccgcgccctg acctacgccg acgacatcgc ccgcaactgt tcgccggcgt cgctggcggt gatcaagcgg caggcctacg acgacgcgct gcgcgacgtc gtcgacgtca gcgcccgcgc cgaaaccctc atgcacgagt cgctgcagcg ccccgacgtc atcgagggca tcaccagctt cctggagaag cggccgccgc ggttcccacc cctggccacc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyavA.00358.f.AE1.GE43229
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 17
PERCENT COVERAGE: 34
Validated AA Sequence
MXXVRXXXXX XXXXXXFXXX XXXXXXGIXW IXXRXAXXXX XXXXLXXXXX XFXXXXXXXG XXXXXVSXXX XXXXXXXXXX XXXXXXXPXX XXXXXRQX
Validated NT Sequence
gnnnnctgac gncnacnacn tnnnnnannn nntnnnctta nnnctgncgn ttgntnncnn nnnnnnnnnn cggnnannnn ttgnnnnnnn nnnnnnnnnn nnnngnnnng nnncnnnnnn nnnnnncgnc ngnngagacn atntcnnnna ncnnnccgan nnnngnnnnc nnntnnnnga anaangngcn nnnnntnann agnangncca nngnnncnnc cnnncnngcc anncgcgncn ggatccannn gatgccnnnn ncnncnatnn nnnnncnnng nangaacgng nngnnaancn nnnnnnntgn nnnnnnnaac cgcacnnnnn acatnnnnnn nnnnntcnnn nnnntgnnnn nncannnncc gtngatnnng ncgatgatcg gncngcacag tgcggtcnng aagtggggat ggcgatnnnn gnccnannng ntcnnnnnnn ngtcncnntn nnncanctnn gcanngatgg atcccgcncc ncncnngnng ncnnncnnnc nnancccncg ncnnnnnncn gtnnnnanna tcnnnngg
Expected Protein Sequence
MSAADHVVTD EAVLYQTTET GVAVVTFNRP ERLNAWGPDI SSGFYACIDR AEADPAVRVI VLTGAGRGFC AGANLGGAGS IDAKLGDGDT DVTALVGDRH PHFLTALRKP IIAAINGACV GIGLTHALMC DVRFAAAGAK FATAFTRRGL IAEYGITWIL PRLAGWGAAM DLLLSGRTFF AEEAAKLGLV NEIVAPDELM ARALTYADDI ARNCSPASLA VIKRQAYDDA LRDVVDVSAR AETLMHESLQ RPDVIEGITS FLEKRPPRFP PLATGHHHHH H
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gagtgccgcc gaccatgtgg tcaccgacga ggcggtgctg taccagacca cggaaaccgg cgttgcggtc gtgacgttca accggcccga gcggctcaac gcctggggac ccgacatctc gagcggcttc tacgcgtgca tcgaccgcgc cgaggcggac cccgcggtcc gggtgatcgt gttgacgggc gccggtcggg ggttctgcgc gggcgccaac ctgggcggcg cgggatccat cgatgcgaag ctgggcgacg gcgacaccga cgtcaccgcg ttggtcggcg atcgccatcc ccacttcctg accgcactgc gcaaaccgat catcgccgcg atcaacggcg cctgcgtcgg catcgggctg acccacgccc tgatgtgcga cgtgcggttc gcggccgccg gcgccaagtt cgccaccgcg ttcacccgcc ggggactgat cgccgagtac ggcatcacct ggatcctgcc gcggctggcc ggctggggcg cggcgatgga cctgctgttg agcgggcgca ccttcttcgc cgaggaggcc gcaaaactcg ggctggtcaa cgagatcgtc gcacccgacg agctgatggc ccgcgccctg acctacgccg acgacatcgc ccgcaactgt tcgccggcgt cgctggcggt gatcaagcgg caggcctacg acgacgcgct gcgcgacgtc gtcgacgtca gcgcccgcgc cgaaaccctc atgcacgagt cgctgcagcg ccccgacgtc atcgagggca tcaccagctt cctggagaag cggccgccgc ggttcccacc cctggccacc gggcaccacc atcatcatca ttaacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat
Details for MyavA.00358.f.A1.PS00580
PURIFICATION DATe: 5/3/2010
CONCENTRATION: 43.87mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSAADHV VTDEAVLYQT TETGVAVVTF NRPERLNAWG PDISSGFYAC IDRAEADPAV RVIVLTGAGR GFCAGANLGG AGSIDAKLGD GDTDVTALVG DRHPHFLTAL RKPIIAAING ACVGIGLTHA LMCDVRFAAA GAKFATAFTR RGLIAEYGIT WILPRLAGWG AAMDLLLSGR TFFAEEAAKL GLVNEIVAPD ELMARALTYA DDIARNCSPA SLAVIKRQAY DDALRDVVDV SARAETLMHE SLQRPDVIEG ITSFLEKRPP RFPPLAT
Validated NT Sequence
gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgagtgccg ccgaccatgt ggtcaccgac gaggcggtgc tgtaccagac cacggaaacc ggcgttgcgg tcgtgacgtt caaccggccc gagcggctca acgcctgggg acccgacatc tcgagcggct tctacgcgtg catcgaccgc gccgaggcgg accccgcggt ccgggtgatc gtgttgacgg gcgccggtcg ggggttctgc gcgggcgcca acctgggcgg cgcgggatcc atcgatgcga agctgggcga cggcgacacc gacgtcaccg cgttggtcgg cgatcgccat ccccacttcc tgaccgcact gcgcaaaccg atcatcgccg cgatcaacgg cgcctgcgtc ggcatcgggc tgacccacgc cctgatgtgc gacgtgcggt tcgcggccgc cggcgccaag ttcgccaccg cgttcacccg ccggggactg atcgccgagt acggcatcac ctggatcctg ccgcggctgg ccggctgggg cgcggcgatg gacctgctgt tgagcgggcg caccttcttc gccgaggagg ccgcaaaact cgggctggtc aacgagatcg tcgcacccga cgagctgatg gcccgcgccc tgacctacgc cgacgacatc gcccgcaact gttcgccggc gtcgctggcg gtgatcaagc ggcaggccta cgacgacgcg ctgcgcgacg tcgtcgacgt cagcgcccgc gccgaaaccc tcatgcacga gtcgctgcag cgccccgacg tcatcgaggg catcaccagc ttcctggaga agcggccgcc gcggttccca cccctggcca cctaataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAADHVVT DEAVLYQTTE TGVAVVTFNR PERLNAWGPD ISSGFYACID RAEADPAVRV IVLTGAGRGF CAGANLGGAG SIDAKLGDGD TDVTALVGDR HPHFLTALRK PIIAAINGAC VGIGLTHALM CDVRFAAAGA KFATAFTRRG LIAEYGITWI LPRLAGWGAA MDLLLSGRTF FAEEAAKLGL VNEIVAPDEL MARALTYADD IARNCSPASL AVIKRQAYDD ALRDVVDVSA RAETLMHESL QRPDVIEGIT SFLEKRPPRF PPLAT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGCCGCC GACCATGTGG TCACCGACGA GGCGGTGCTG TACCAGACCA CGGAAACCGG CGTTGCGGTC GTGACGTTCA ACCGGCCCGA GCGGCTCAAC GCCTGGGGAC CCGACATCTC GAGCGGCTTC TACGCGTGCA TCGACCGCGC CGAGGCGGAC CCCGCGGTCC GGGTGATCGT GTTGACGGGC GCCGGTCGGG GGTTCTGCGC GGGCGCCAAC CTGGGCGGCG CGGGATCCAT CGATGCGAAG CTGGGCGACG GCGACACCGA CGTCACCGCG TTGGTCGGCG ATCGCCATCC CCACTTCCTG ACCGCACTGC GCAAACCGAT CATCGCCGCG ATCAACGGCG CCTGCGTCGG CATCGGGCTG ACCCACGCCC TGATGTGCGA CGTGCGGTTC GCGGCCGCCG GCGCCAAGTT CGCCACCGCG TTCACCCGCC GGGGACTGAT CGCCGAGTAC GGCATCACCT GGATCCTGCC GCGGCTGGCC GGCTGGGGCG CGGCGATGGA CCTGCTGTTG AGCGGGCGCA CCTTCTTCGC CGAGGAGGCC GCAAAACTCG GGCTGGTCAA CGAGATCGTC GCACCCGACG AGCTGATGGC CCGCGCCCTG ACCTACGCCG ACGACATCGC CCGCAACTGT TCGCCGGCGT CGCTGGCGGT GATCAAGCGG CAGGCCTACG ACGACGCGCT GCGCGACGTC GTCGACGTCA GCGCCCGCGC CGAAACCCTC ATGCACGAGT CGCTGCAGCG CCCCGACGTC ATCGAGGGCA TCACCAGCTT CCTGGAGAAG CGGCCGCCGC GGTTCCCACC CCTGGCCACC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA