CENTER ID: | MyavA.00358.f |
ORGANISM: | Mycobacterium avium 104 |
ASSOCIATED DISEASE: | Johne's disease, paratuberculosis |
CURRENT STATUS: | native diffraction-data |
COMMUNITY REQUEST: | False |
NIH RISK GROUP: | 2 |
SELECT AGENT: | False |
NIH PRIORITY pathogens category: |
IIIC |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
MyavA.00358.f.A1.GE28523 | Full length( MyavA.00358.f ) | 1 | 274 | ||
MyavA.00358.f.AE1.GE43229 | Full length( MyavA.00358.f ) | 1 | 274 |
CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
---|---|---|---|---|---|
MyavA.00358.f.A1.PS00580 | Full length( MyavA.00358.f ) | 1 | 274 |
RESOURCE | REFERENCE ID |
---|---|
BV-BRC: | fig|243243.7.peg.1552 |
RefSeq: | YP_880824.1 |
UniProt: | A0A0H3A2H0 |
HARVESTED ON: | 2/9/2010 |
SEQUENCED ON: | 2/22/2010 |
EXPECTED MW: | 31kDa |
OBSERVED MW: | 35kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | High Expression |
EXPRESSION HOST: | data unavailable |
SEQUENCING RESULT: | pass |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 97 |
HARVESTED ON: | 6/12/2019 |
SEQUENCED ON: | 6/18/2019 |
EXPECTED MW: | 30kDa |
OBSERVED MW: | 30kDa |
ANTIBIOTIC MARKER: | ampicillin |
COUNT OF EXPRESSION COLONIES: | Good (10-50) |
TOTAL EXPRESSION LEVEL: | Low Expression |
SOLUBLE EXPRESSION LEVEL | Low Expression |
EXPRESSION HOST: | BL 21 (DE3) Rosetta |
SEQUENCING RESULT: | |
PERCENT IDENTITY: | 17 |
PERCENT COVERAGE: | 34 |
PURIFICATION DATe: | 5/3/2010 |
CONCENTRATION: | 43.87mg/ml |
OBSERVED MW: | data unavailable |
EXPRESSION LEVEL: | Moderate Expression |
PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
EXPRESSION HOST: | data unavailable |
VIAL COUNT (approx.): | 9 |
VIAL VOLUME: | 200µl |
PERCENT IDENTITY: | 100 |
PERCENT COVERAGE: | 97 |