MytuD.00247.a

PROBABLE ACYL-CoA DEHYDROGENASE FADE20

CENTER ID: MytuD.00247.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00247.a.A1.GE25817 Full length( MytuD.00247.a ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.3035
RefSeq: NP_217240.1
TubercuList: Rv2724c
MTB Network Portal: Rv2724c
UniProt: O33229

Orthologues for Rv2724c

Species % Identity % Coverage Clones Proteins Structures
marinum 92 100 1 1 0
avium 91 100 0 0 0
ulcerans 91 100 1 0 0
abscessus 77 99 1 1 0
smegmatis 58 98 1 1 0
avium 57 98 0 0 0
avium 57 97 0 0 0
paratuberculosis 57 97 1 0 0
avium 56 98 0 0 0
marinum 56 98 1 0 0
abscessus 55 98 1 1 0
abscessus 54 95 1 1 0
avium 53 98 0 0 0
marinum 53 95 1 0 0
smegmatis 51 98 1 0 0
thermoresistibile 51 98 1 1 0
paratuberculosis 51 96 1 1 0
avium 50 98 0 0 0
avium 50 98 1 0 0
avium 49 98 0 0 0
thermoresistibile 49 98 1 0 0
thermoresistibile 49 82 0 0 0
abscessus 48 96 1 0 0
thermoresistibile 46 98 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSATKYQRT LFEPEHELFR ESYRAFLDRH VAPYHDEWEK TKIVDRGVWL EAGKQGFLGM AVPEEYGGGG NADFRYNTVI TEETCAGRYS GIGFGLHNDI VAPYLLALAT EEQKRRWFPN FCTGELITAI AMTEPGTGSD LQGITTRAVK HGDHYVLNGS KTFITNGINS DLVIVVAQTD PEKGAQGFSL LVVERGMAGF ERGRQLDKIG LDAQDTAELS FTDVAVPAEN LLGQEGMGFI YLMQNLPQER ISIAIMAAAG MESVLEQTLQ YAKERKAFGR SIGSFQNSRF LLAELATEAT VVRIMVDEFI KLHLAGKLTA EQAAMAKWYA TEKQVYLNDR CLQLHGGYGY MREYPVARAY LDSRVQTIYG GTTEIMKEII GRGLGV
NT Sequence
ATGGGCAGTG CCACCAAGTA CCAGCGCACA CTTTTCGAAC CCGAACATGA GTTGTTTCGC GAGTCTTACC GGGCGTTCCT CGATCGCCAC GTCGCGCCCT ATCACGATGA ATGGGAAAAG ACCAAGATCG TCGACCGCGG CGTGTGGCTG GAGGCCGGCA AGCAGGGATT TCTGGGCATG GCGGTGCCCG AGGAGTATGG CGGTGGGGGT AACGCGGACT TCCGGTACAA CACGGTCATC ACCGAGGAAA CCTGCGCCGG ACGGTACAGC GGGATCGGTT TTGGCCTGCA CAACGACATC GTGGCGCCGT ACTTGCTGGC GCTGGCCACC GAAGAGCAGA AGCGGCGCTG GTTTCCCAAC TTCTGCACCG GGGAATTGAT CACCGCGATC GCGATGACCG AGCCCGGAAC CGGTAGTGAC CTGCAAGGTA TCACCACCCG TGCCGTCAAA CACGGTGATC ATTACGTGCT AAACGGGTCA AAGACGTTCA TCACCAACGG GATCAATTCA GACCTAGTGA TCGTGGTGGC GCAGACGGAT CCCGAAAAGG GTGCGCAAGG GTTCAGTTTG TTGGTCGTCG AACGCGGCAT GGCTGGATTC GAACGCGGCC GCCAGCTGGA CAAGATCGGG TTGGACGCCC AGGACACCGC CGAGCTGTCC TTCACCGACG TGGCGGTTCC AGCCGAAAAC CTGCTCGGCC AGGAGGGGAT GGGGTTCATC TACTTGATGC AGAACCTGCC GCAAGAACGG ATCTCGATCG CCATCATGGC GGCCGCGGGG ATGGAATCCG TGCTGGAGCA GACACTGCAG TACGCCAAGG AGCGCAAGGC GTTCGGCAGG TCGATCGGTA GCTTCCAGAA CAGCCGATTC TTGCTGGCCG AGCTGGCAAC CGAGGCCACT GTGGTGCGCA TCATGGTCGA CGAATTCATT AAGCTGCACC TGGCGGGGAA GTTGACGGCA GAGCAGGCCG CCATGGCCAA GTGGTATGCC ACCGAGAAGC AGGTGTATCT GAACGACCGC TGTCTGCAGC TGCACGGTGG GTACGGCTAC ATGCGCGAAT ACCCAGTCGC CCGGGCCTAT CTCGATTCGC GGGTACAGAC GATTTACGGT GGCACGACCG AGATCATGAA AGAGATCATC GGTCGTGGCT TGGGTGTT
Details for MytuD.00247.a.A1.GE25817
HARVESTED ON: 11/19/2008
SEQUENCED ON: 8/18/2010
EXPECTED MW: 45kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGSATKYQR TLFEPEHELF RESYRAFLDR HVAPYHDEWE KTKIVDRGVW LEAGKQGFLG MAVPEEYGGG GNADFRYNTV ITEETCAGRY SGIGFGLHND IVAPYLLALA TEEQKRRWFP NFCTGELITA IAMTEPGTGS DLQGITTRAV KHGDHYVLNG SKTFITNGIN SDLVIVVAQT DPEKGAQGFS LLVVERGMAG FERGRQLDKI GLDAQDTAEL SFTDVAVPAE NLLGQEGMGF IYLMQNLPQE RISIAIMAAA GMESVLEQTL QYAKERKAFG RSIGSFQNSR FLLAELATEA TVVRIMVDEF IKLHLAGKLT AEQAAMAKWY ATEKQVYLND RCLQLHGGYG YMREYPVARA YLDSRVQTIY GGTTEIMKEI IGRGLGV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggca gtgccaccaa gtaccagcgc acacttttcg aacccgaaca tgagttgttt cgcgagtctt accgggcgtt cctcgatcgc cacgtcgcgc cctatcacga tgaatgggaa aagaccaaga tcgtcgaccg cggcgtgtgg ctggaggccg gcaagcaggg atttctgggc atggcggtgc ccgaggagta tggcggtggg ggtaacgcgg acttccggta caacacggtc atcaccgagg aaacctgcgc cggacggtac agcgggatcg gttttggcct gcacaacgac atcgtggcgc cgtacttgct ggcgctggcc accgaagagc agaagcggcg ctggtttccc aacttctgca ccggggaatt gatcaccgcg atcgcgatga ccgagcccgg aaccggtagt gacctgcaag gtatcaccac ccgtgccgtc aaacacggtg atcattacgt gctaaacggg tcaaagacgt tcatcaccaa cgggatcaat tcagacctag tgatcgtggt ggcgcagacg gatcccgaaa agggtgcgca agggttcagt ttgttggtcg tcgaacgcgg catggctgga ttcgaacgcg gccgccagct ggacaagatc gggttggacg cccaggacac cgccgagctg tccttcaccg acgtggcggt tccagccgaa aacctgctcg gccaggaggg gatggggttc atctacttga tgcagaacct gccgcaagaa cggatctcga tcgccatcat ggcggccgcg gggatggaat ccgtgctgga gcagacactg cagtacgcca aggagcgcaa ggcgttcggc aggtcgatcg gtagcttcca gaacagccga ttcttgctgg ccgagctggc aaccgaggcc actgtggtgc gcatcatggt cgacgaattc attaagctgc acctggcggg gaagttgacg gcagagcagg ccgccatggc caagtggtat gccaccgaga agcaggtgta tctgaacgac cgctgtctgc agctgcacgg tgggtacggc tacatgcgcg aatacccagt cgcccgggcc tatctcgatt cgcgggtaca gacgatttac ggtggcacga ccgagatcat gaaagagatc atcggtcgtg gcttgggtgt ttaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSATKYQR TLFEPEHELF RESYRAFLDR HVAPYHDEWE KTKIVDRGVW LEAGKQGFLG MAVPEEYGGG GNADFRYNTV ITEETCAGRY SGIGFGLHND IVAPYLLALA TEEQKRRWFP NFCTGELITA IAMTEPGTGS DLQGITTRAV KHGDHYVLNG SKTFITNGIN SDLVIVVAQT DPEKGAQGFS LLVVERGMAG FERGRQLDKI GLDAQDTAEL SFTDVAVPAE NLLGQEGMGF IYLMQNLPQE RISIAIMAAA GMESVLEQTL QYAKERKAFG RSIGSFQNSR FLLAELATEA TVVRIMVDEF IKLHLAGKLT AEQAAMAKWY ATEKQVYLND RCLQLHGGYG YMREYPVARA YLDSRVQTIY GGTTEIMKEI IGRGLGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcagtgcc accaagtacc agcgcacact tttcgaaccc gaacatgagt tgtttcgcga gtcttaccgg gcgttcctcg atcgccacgt cgcgccctat cacgatgaat gggaaaagac caagatcgtc gaccgcggcg tgtggctgga ggccggcaag cagggatttc tgggcatggc ggtgcccgag gagtatggcg gtgggggtaa cgcggacttc cggtacaaca cggtcatcac cgaggaaacc tgcgccggac ggtacagcgg gatcggtttt ggcctgcaca acgacatcgt ggcgccgtac ttgctggcgc tggccaccga agagcagaag cggcgctggt ttcccaactt ctgcaccggg gaattgatca ccgcgatcgc gatgaccgag cccggaaccg gtagtgacct gcaaggtatc accacccgtg ccgtcaaaca cggtgatcat tacgtgctaa acgggtcaaa gacgttcatc accaacggga tcaattcaga cctagtgatc gtggtggcgc agacggatcc cgaaaagggt gcgcaagggt tcagtttgtt ggtcgtcgaa cgcggcatgg ctggattcga acgcggccgc cagctggaca agatcgggtt ggacgcccag gacaccgccg agctgtcctt caccgacgtg gcggttccag ccgaaaacct gctcggccag gaggggatgg ggttcatcta cttgatgcag aacctgccgc aagaacggat ctcgatcgcc atcatggcgg ccgcggggat ggaatccgtg ctggagcaga cactgcagta cgccaaggag cgcaaggcgt tcggcaggtc gatcggtagc ttccagaaca gccgattctt gctggccgag ctggcaaccg aggccactgt ggtgcgcatc atggtcgacg aattcattaa gctgcacctg gcggggaagt tgacggcaga gcaggccgcc atggccaagt ggtatgccac cgagaagcag gtgtatctga acgaccgctg tctgcagctg cacggtgggt acggctacat gcgcgaatac ccagtcgccc gggcctatct cgattcgcgg gtacagacga tttacggtgg cacgaccgag atcatgaaag agatcatcgg tcgtggcttg ggtgttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa