MysmA.00247.c

Acyl-CoA dehydrogenase

CENTER ID: MysmA.00247.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00247.c.A1.GE28588 Full length( MysmA.00247.c ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4716
RefSeq: YP_889088.1
UniProt: A0R1Q0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRNLFTEDH EAFRTLARDF IEKEVVSAYP EWEKAGRMPR DVFKQMGELG MLGMAIPEEY GGAGIPDYRY NVVLQEEAAR ALVTLSTVRT QLEVILPYFL HYADTEQRQR WFPGLAAGTL LTAIAMTEPG TGSDLAGVRT TAVRDGDHYV VNGAKTFITG GIQADLVVVV ARTSTDPDNR RKGLTLLVVE DGMAGFSRGR ELEKMGCKVQ DTAELSFTDV RVPVANVLGE EGEAFSYLGH NLAQERLTVA VGSVAQARSA IDAAIDYTKT RKAFGTPVAS FQNTKFELAA CSAEVEAAQA MLDRAVALHV DGELSGADAA RVKLFCTEMQ ARVVDRCLQL FGGYGYMMEY PIARLYTDAR VARIYAGTSE VMKVIIAKSL GL
NT Sequence
ATGCAGCGGA ATCTGTTCAC CGAGGATCAC GAGGCATTTC GCACGCTGGC GCGGGATTTC ATCGAAAAGG AAGTCGTCTC GGCCTATCCC GAATGGGAGA AGGCCGGACG CATGCCGCGC GACGTGTTCA AGCAGATGGG TGAACTGGGC ATGCTCGGCA TGGCCATCCC CGAGGAGTAC GGCGGCGCAG GCATTCCCGA TTACCGCTAC AACGTCGTGC TGCAGGAGGA GGCCGCCAGG GCCCTGGTGA CCCTGTCCAC GGTCCGCACA CAACTCGAGG TGATCCTGCC GTACTTCCTG CACTACGCCG ACACCGAGCA GCGGCAGCGG TGGTTCCCCG GTCTGGCGGC CGGCACGCTG CTGACCGCCA TCGCGATGAC CGAACCGGGG ACCGGATCGG ATCTCGCGGG CGTGCGCACC ACGGCCGTGC GGGACGGGGA CCACTACGTC GTCAACGGCG CCAAGACCTT CATTACCGGT GGTATCCAGG CGGATCTGGT TGTCGTGGTG GCCCGCACGT CCACCGATCC GGATAACCGC CGCAAGGGCC TGACACTGCT GGTGGTCGAG GACGGCATGG CGGGCTTCAG CCGCGGCCGC GAGCTGGAGA AGATGGGCTG CAAGGTGCAG GACACCGCGG AACTGTCGTT CACCGACGTG CGGGTGCCGG TGGCCAACGT GCTGGGGGAG GAGGGCGAGG CGTTCAGCTA CCTCGGCCAC AACCTGGCGC AGGAGCGCCT CACGGTCGCC GTCGGTTCGG TGGCCCAGGC CCGCTCGGCG ATCGACGCGG CGATCGATTA CACCAAGACC CGCAAGGCAT TCGGCACGCC CGTGGCGTCT TTCCAGAACA CCAAATTCGA GCTGGCGGCG TGTTCGGCGG AGGTCGAGGC AGCCCAGGCC ATGCTCGACC GCGCGGTGGC GCTGCACGTC GACGGCGAGC TCTCCGGGGC CGACGCGGCG CGCGTCAAAC TGTTCTGCAC CGAGATGCAG GCGCGCGTGG TCGATCGTTG CCTGCAGCTG TTCGGCGGGT ACGGCTACAT GATGGAGTAC CCCATCGCCA GGCTGTACAC CGACGCCCGC GTCGCCCGGA TCTACGCGGG TACCAGCGAG GTCATGAAAG TCATCATCGC GAAGTCGCTG GGCCTG
Details for MysmA.00247.c.A1.GE28588
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 44kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MQRNLFTEDH EAFRTLARDF IEKEVVSAYP EWEKAGRMPR DVFKQMGELG MLGMAIPEEY GGAGIPDYRY NVVLQEEAAR ALVTLSTVRT QLEVILPYFL HYADTEQRQR WFPGLAAGTL LTAIAMTEPG TGSDLAGVRT TAVRDGDHYV VNGAKTFITG GIQADLVVVV ARTSTDPDNR RKGLTLLVVE DGMAGFSRGR ELEKMGCKVQ DTAELSFTDV RVPVANVLGE EGEAFSYLGH NLAQERLTVA VGSVAQARSA IDAAIDYTKT RKAFGTPVAS FQNTKFELAA CSAEVEAAQA MLDRAVALHV DGELSGADAA RVKLFCTEMQ ARVVDRCLQL FGGYGYMMEY PIARLYTDAR VARIYAGTSE VMKVIIAKSL GL
Validated NT Sequence
ctggaagctc agacccaggg tcctggttcg atgcagcgga atctgttcac cgaggatcac gaggcatttc gcacgctggc gcgggatttc atcgaaaagg aagtcgtctc ggcctatccc gaatgggaga aggccggacg catgccgcgc gacgtgttca agcagatggg tgaactgggc atgctcggca tggccatccc cgaggagtac ggcggcgcag gcattcccga ttaccgctac aacgtcgtgc tgcaggagga ggccgccagg gccctggtga ccctgtccac ggtccgcaca caactcgagg tgatcctgcc gtacttcctg cactacgccg acaccgagca gcggcagcgg tggttccccg gtctggcggc cggcacgctg ctgaccgcca tcgcgatgac cgaaccgggg accggatcgg atctcgcggg cgtgcgcacc acggccgtgc gggacgggga ccactacgtc gtcaacggcg ccaagacctt cattaccggt ggtatccagg cggatctggt tgtcgtggtg gcccgcacgt ccaccgatcc ggataaccgc cgcaagggcc tgacactgct ggtggtcgag gacggcatgg cgggcttcag ccgcggccgc gagctggaga agatgggctg caaggtgcag gacaccgcgg aactgtcgtt caccgacgtg cgggtgccgg tggccaacgt gctgggggag gagggcgagg cgttcagcta cctcggccac aacctggcgc aggagcgcct cacggtcgcc gtcggttcgg tggcccaggc ccgctcggcg atcgacgcgg cgatcgatta caccaagacc cgcaaggcat tcggcacgcc cgtggcgtct ttccagaaca ccaaattcga gctggcggcg tgttcggcgg aggtcgaggc agcccaggcc atgctcgacc gcgcggtggc gctgcacgtc gacggcgagc tctccggggc cgacgcggcg cgcgtcaaac tgttctgcac cgagatgcag gcgcgcgtgg tcgatcgttg cctgcagctg ttcggcgggt acggctacat gatggagtac cccatcgcca ggctgtacac cgacgcccgc gtcgcccgga tctacgcggg taccagcgag gtcatgaaag tcatcatcgc gaagtcgctg ggcctgta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRNLFTED HEAFRTLARD FIEKEVVSAY PEWEKAGRMP RDVFKQMGEL GMLGMAIPEE YGGAGIPDYR YNVVLQEEAA RALVTLSTVR TQLEVILPYF LHYADTEQRQ RWFPGLAAGT LLTAIAMTEP GTGSDLAGVR TTAVRDGDHY VVNGAKTFIT GGIQADLVVV VARTSTDPDN RRKGLTLLVV EDGMAGFSRG RELEKMGCKV QDTAELSFTD VRVPVANVLG EEGEAFSYLG HNLAQERLTV AVGSVAQARS AIDAAIDYTK TRKAFGTPVA SFQNTKFELA ACSAEVEAAQ AMLDRAVALH VDGELSGADA ARVKLFCTEM QARVVDRCLQ LFGGYGYMME YPIARLYTDA RVARIYAGTS EVMKVIIAKS LGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagcggaat ctgttcaccg aggatcacga ggcatttcgc acgctggcgc gggatttcat cgaaaaggaa gtcgtctcgg cctatcccga atgggagaag gccggacgca tgccgcgcga cgtgttcaag cagatgggtg aactgggcat gctcggcatg gccatccccg aggagtacgg cggcgcaggc attcccgatt accgctacaa cgtcgtgctg caggaggagg ccgccagggc cctggtgacc ctgtccacgg tccgcacaca actcgaggtg atcctgccgt acttcctgca ctacgccgac accgagcagc ggcagcggtg gttccccggt ctggcggccg gcacgctgct gaccgccatc gcgatgaccg aaccggggac cggatcggat ctcgcgggcg tgcgcaccac ggccgtgcgg gacggggacc actacgtcgt caacggcgcc aagaccttca ttaccggtgg tatccaggcg gatctggttg tcgtggtggc ccgcacgtcc accgatccgg ataaccgccg caagggcctg acactgctgg tggtcgagga cggcatggcg ggcttcagcc gcggccgcga gctggagaag atgggctgca aggtgcagga caccgcggaa ctgtcgttca ccgacgtgcg ggtgccggtg gccaacgtgc tgggggagga gggcgaggcg ttcagctacc tcggccacaa cctggcgcag gagcgcctca cggtcgccgt cggttcggtg gcccaggccc gctcggcgat cgacgcggcg atcgattaca ccaagacccg caaggcattc ggcacgcccg tggcgtcttt ccagaacacc aaattcgagc tggcggcgtg ttcggcggag gtcgaggcag cccaggccat gctcgaccgc gcggtggcgc tgcacgtcga cggcgagctc tccggggccg acgcggcgcg cgtcaaactg ttctgcaccg agatgcaggc gcgcgtggtc gatcgttgcc tgcagctgtt cggcgggtac ggctacatga tggagtaccc catcgccagg ctgtacaccg acgcccgcgt cgcccggatc tacgcgggta ccagcgaggt catgaaagtc atcatcgcga agtcgctggg cctgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa