MypaA.00247.b

FadE20_3

CENTER ID: MypaA.00247.b
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.00247.b.A1.GE29279 Full length( MypaA.00247.b ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4128
RefSeq: NP_962811.1
UniProt: Q73T42

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAAHLQYTSE HHQFRELVRD FVQRTVVPNH EKWERDGQWD RSLFVEAGKL GLLGFSVPER LGGPGVDDFR YNAIVVDELQ RAGAAAEAIA FSLQNDIVLP YLVELTTPEQ QRRWLPGVVT GETVLGIAMT EPGTGSDLAG IRTTAVRDGD HYLVNGAKTF ISNGQVGDLF VVAVRTAPDR HKGLSLLVVD ADTPGFGRGR NLEKIGLHAQ DTSELSFTDM RVPAENLLEA EGRGFYQLMK NLPQERLSLA VGAVAAAEAV FAETLDYVKQ WHAFGAPIAA FQNSQFVLAE LATEIDVART YLDDCLAAHL AGELTAARAA RLKWWTTDLQ VRTADRCLQL HGGYGYMREY GVARAFVDAR IQTIYGGTNE IMKTIIAKDL GL
NT Sequence
ATGGCCGCAC ACCTGCAGTA CACCTCCGAG CATCACCAGT TCCGGGAGCT GGTCCGCGAT TTCGTGCAAC GGACGGTCGT GCCCAATCAC GAGAAGTGGG AACGCGACGG CCAGTGGGAC CGGTCGCTGT TCGTCGAGGC AGGCAAACTG GGGCTGCTGG GCTTTTCGGT GCCCGAGCGG TTGGGCGGCC CGGGGGTCGA CGACTTTCGC TACAACGCGA TCGTGGTCGA CGAGTTGCAG CGGGCCGGCG CGGCCGCCGA GGCCATCGCC TTCTCGCTGC AGAACGACAT CGTGCTGCCC TACCTCGTCG AGCTGACCAC ACCCGAGCAA CAACGGCGCT GGCTGCCCGG CGTGGTGACC GGCGAGACGG TGCTCGGGAT CGCCATGACC GAGCCCGGCA CCGGCAGCGA CCTGGCCGGG ATCCGCACCA CCGCGGTGCG AGACGGCGAT CACTACCTGG TCAACGGCGC CAAGACGTTC ATCTCCAACG GGCAGGTGGG CGACCTGTTC GTGGTCGCGG TGCGCACCGC ACCGGACCGG CACAAGGGGC TTTCGCTGCT GGTCGTGGAC GCGGACACGC CGGGCTTCGG CCGGGGCCGC AACCTGGAGA AGATCGGCCT GCACGCCCAG GACACCAGCG AGCTGAGCTT CACCGACATG CGGGTGCCGG CCGAGAACCT GCTCGAGGCG GAAGGCCGGG GCTTCTACCA GCTGATGAAA AACCTTCCCC AGGAACGGCT TTCGCTGGCC GTGGGCGCGG TCGCCGCCGC CGAGGCAGTA TTCGCCGAGA CGCTGGACTA CGTCAAGCAG TGGCACGCCT TCGGCGCGCC CATCGCCGCC TTCCAGAACA GTCAATTCGT GCTCGCCGAA CTGGCCACCG AAATCGACGT TGCCCGCACT TATCTCGACG ACTGCCTGGC CGCGCACCTG GCCGGCGAGC TGACCGCGGC GCGGGCCGCC CGGCTGAAAT GGTGGACCAC CGATCTGCAG GTCCGCACCG CCGACCGCTG CCTGCAGTTG CACGGCGGAT ACGGCTACAT GCGCGAGTAC GGCGTGGCGC GGGCGTTCGT CGACGCCCGC ATCCAGACCA TCTACGGCGG CACCAACGAG ATCATGAAGA CCATCATCGC CAAGGACCTG GGCCTC
Details for MypaA.00247.b.A1.GE29279
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 44kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAAHLQYTSE HHQFRELVRD FVQRTVVPNH EKWERDGQWD RSLFVEAGKL GLLGFSVPER LGGPGVDDFR YNAIVVDELQ RAGAAAEAIA FSLQNDIVLP YLVELTTPEQ QRRWLPGVVT GETVLGIAMT EPGTGSDLAG IRTTAVRDGD HYLVNGAKTF ISNGQVGDLF VVAVRTAPDR HKGLSLLVVD ADTPGFGRGR NLEKIGLHAQ DTSELSFTDM RVPAENLLEA EGRGFYQLMK NLPQERLSLA VGAVAAAEAV FAETLDYVKQ WHAFGAPIAA FQNSQFVLAE LATEIDVART YLDDCLAAHL AGELTAARAA RLKWWTTDLQ VRTADRCLQL HGGYGYMREY GVARAFVDAR IQTIYGGTNE IMKTIIAKDL GL
Validated NT Sequence
ttattagagg cccaggtcct tggcgatgat ggtcttcatg atctcgttgg tgccgccgta gatggtctgg atgcgggcgt cgacgaacgc ccgcgccacg ccgtactcgc gcatgtagcc gtatccgccg tgcaactgca ggcagcggtc ggcggtgcgg acctgcagat cggtggtcca ccatttcagc cgggcggccc gcgccgcggt cagctcgccg gccaggtgcg cggccaggca gtcgtcgaga taagtgcggg caacgtcgat ttcggtggcc agttcggcga gcacgaattg actgttctgg aaggcggcga tgggcgcgcc gaaggcgtgc cactgcttga cgtagtccag cgtctcggcg aatactgcct cggcggcggc gaccgcgccc acggccagcg aaagccgttc ctggggaagg tttttcatca gctggtagaa gccccggcct tccgcctcga gcaggttctc ggccggcacc cgcatgtcgg tgaagctcag ctcgctggtg tcctgggcgt gcaggccgat cttctccagg ttgcggcccc ggccgaagcc cggcgtgtcc gcgtccacga ccagcagcga aagccccttg tgccggtccg gtgcggtgcg caccgcgacc acgaacaggt cgcccacctg cccgttggag atgaacgtct tggcgccgtt gaccaggtag tgatcgccgt ctcgcaccgc ggtggtgcgg atcccggcca ggtcgctgcc ggtgccgggc tcggtcatgg cgatcccgag caccgtctcg ccggtcacca cgccgggcag ccagcgccgt tgttgctcgg gtgtggtcag ctcgacgagg tagggcagca cgatgtcgtt ctgcagcgag aaggcgatgg cctcggcggc cgcgccggcc cgctgcaact cgtcgaccac gatcgcgttg tagcgaaagt cgtcgacccc cgggccgccc aaccgctcgg gcaccgaaaa gcccagcagc cccagtttgc ctgcctcgac gaacagcgac cggtcccact ggccgtcgcg ttcccacttc tcgtgattgg gcacgaccgt ccgttgcacg aaatcgcgga ccagctcccg gaactggtga tgctcggagg tgtactgcag gtgtgcggcc atcgaaccag gaccctgggt ctgagcttcc ag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAAHLQYTS EHHQFRELVR DFVQRTVVPN HEKWERDGQW DRSLFVEAGK LGLLGFSVPE RLGGPGVDDF RYNAIVVDEL QRAGAAAEAI AFSLQNDIVL PYLVELTTPE QQRRWLPGVV TGETVLGIAM TEPGTGSDLA GIRTTAVRDG DHYLVNGAKT FISNGQVGDL FVVAVRTAPD RHKGLSLLVV DADTPGFGRG RNLEKIGLHA QDTSELSFTD MRVPAENLLE AEGRGFYQLM KNLPQERLSL AVGAVAAAEA VFAETLDYVK QWHAFGAPIA AFQNSQFVLA ELATEIDVAR TYLDDCLAAH LAGELTAARA ARLKWWTTDL QVRTADRCLQ LHGGYGYMRE YGVARAFVDA RIQTIYGGTN EIMKTIIAKD LGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgcacac ctgcagtaca cctccgagca tcaccagttc cgggagctgg tccgcgattt cgtgcaacgg acggtcgtgc ccaatcacga gaagtgggaa cgcgacggcc agtgggaccg gtcgctgttc gtcgaggcag gcaaactggg gctgctgggc ttttcggtgc ccgagcggtt gggcggcccg ggggtcgacg actttcgcta caacgcgatc gtggtcgacg agttgcagcg ggccggcgcg gccgccgagg ccatcgcctt ctcgctgcag aacgacatcg tgctgcccta cctcgtcgag ctgaccacac ccgagcaaca acggcgctgg ctgcccggcg tggtgaccgg cgagacggtg ctcgggatcg ccatgaccga gcccggcacc ggcagcgacc tggccgggat ccgcaccacc gcggtgcgag acggcgatca ctacctggtc aacggcgcca agacgttcat ctccaacggg caggtgggcg acctgttcgt ggtcgcggtg cgcaccgcac cggaccggca caaggggctt tcgctgctgg tcgtggacgc ggacacgccg ggcttcggcc ggggccgcaa cctggagaag atcggcctgc acgcccagga caccagcgag ctgagcttca ccgacatgcg ggtgccggcc gagaacctgc tcgaggcgga aggccggggc ttctaccagc tgatgaaaaa ccttccccag gaacggcttt cgctggccgt gggcgcggtc gccgccgccg aggcagtatt cgccgagacg ctggactacg tcaagcagtg gcacgccttc ggcgcgccca tcgccgcctt ccagaacagt caattcgtgc tcgccgaact ggccaccgaa atcgacgttg cccgcactta tctcgacgac tgcctggccg cgcacctggc cggcgagctg accgcggcgc gggccgcccg gctgaaatgg tggaccaccg atctgcaggt ccgcaccgcc gaccgctgcc tgcagttgca cggcggatac ggctacatgc gcgagtacgg cgtggcgcgg gcgttcgtcg acgcccgcat ccagaccatc tacggcggca ccaacgagat catgaagacc atcatcgcca aggacctggg cctcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa