MymaA.00247.a

Acyl-CoA dehydrogenase

CENTER ID: MymaA.00247.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00247.a.A1.GE29971 Full length( MymaA.00247.a ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.748
RefSeq: YP_001849011.1
UniProt: B2HPX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSRPEYTPE HHQFRQLVRD FVHRIVVPAH EPGERRGQWD RALFTEAARL GLLGFSVPER FGGPGVNDFR YNAIVIDELQ RAGAASEAIA FTLQNDVVLP YLTDLTTLEQ QQRWLPGVVT GDTVLGIAMT EPGAGSDLAG IHTTAVRDGS HYVVNGAKTF ISNGQSGDLF VIAVRTSPER HQGLSLLVVD ATTPGFHRGR SLEKIGLHAQ DTSELSFTDM RVPVENLLQN EGMGFYQLMR NLPQERLALA VGAVAAAEGI LAETLDYVRQ RKAFGSPIAS FQHSQFLLAE LATEIDIART YLDDCLAQHL AGELTAARAA RLKWWTTELQ VRTADRCLQL HGGYGYMREY RVSQAFVDAR IQTIYGGTNE IMKTIIAKDL GI
NT Sequence
ATGACCTCCC GGCCCGAGTA CACACCCGAG CATCACCAGT TCCGGCAATT GGTCCGCGAT TTCGTCCACC GCATCGTCGT CCCGGCCCAC GAACCCGGGG AACGCCGGGG GCAGTGGGAT CGGGCACTGT TCACCGAAGC CGCAAGGCTG GGGCTGCTGG GATTCTCGGT TCCGGAACGG TTCGGCGGAC CGGGAGTGAA CGACTTCCGC TACAACGCGA TCGTCATCGA CGAACTGCAG CGAGCCGGCG CCGCATCCGA AGCCATCGCC TTCACCCTGC AAAACGATGT CGTGCTGCCC TATCTCACCG ACCTGACCAC ACTCGAACAG CAACAACGCT GGCTACCCGG CGTGGTGACC GGTGACACCG TGCTCGGCAT CGCGATGACC GAACCCGGCG CCGGCAGCGA CCTGGCCGGC ATCCACACGA CAGCGGTACG AGACGGTTCG CACTACGTTG TCAACGGCGC CAAGACATTC ATCTCCAACG GTCAAAGCGG CGACCTGTTC GTCATCGCGG TGCGCACCTC ACCGGAACGC CACCAGGGGC TCTCGCTGCT GGTGGTGGAC GCAACCACAC CGGGCTTTCA CCGCGGCCGC AGTCTGGAGA AGATCGGCCT GCACGCCCAG GACACCAGCG AACTCAGTTT CACCGATATG CGGGTTCCCG TGGAGAACCT GTTGCAGAAC GAGGGCATGG GGTTCTACCA GCTCATGCGG AACCTGCCGC AGGAGCGACT GGCCTTGGCA GTGGGCGCGG TCGCCGCCGC CGAAGGCATC TTGGCCGAGA CTCTGGACTA CGTGCGGCAG CGCAAAGCCT TCGGTTCGCC GATCGCCAGC TTTCAGCACA GCCAGTTCCT GCTGGCCGAA CTAGCCACTG AGATCGACAT CGCCCGAACC TATCTCGATG ACTGCCTGGC CCAACACCTC GCCGGCGAGC TGACCGCCGC CCGCGCGGCA CGGCTGAAAT GGTGGACCAC CGAACTTCAG GTGCGTACCG CAGACCGCTG CCTACAACTG CATGGCGGCT ACGGCTATAT GCGGGAATAC CGGGTGTCGC AAGCATTTGT CGATGCCCGC ATCCAGACCA TCTACGGCGG CACCAACGAA ATCATGAAGA CCATCATCGC CAAGGACTTG GGTATC
Details for MymaA.00247.a.A1.GE29971
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MTSRPEYTPE HHQFRQLVRD FVHRIVVPAH EPGERRGQWD RALFTEAARL GLLGFSVPER FGGPGVNDFR YNAIVIDELQ RAGAASEAIA FTLQNDVVLP YLTDLTTLEQ QQRWLPGVVT GDTVLGIAMT EPGAGSDLAG IHTTAVRDGS HYVVNGAKTF ISNGQSGDLF VIAVRTSPER HQGLSLLVVD ATTPGFHRGR SLEKIGLHAQ DTSELSFTDM RVPVENLLQN EGMGFYQLMR NLPQERLALA VGAVAAAEGI LAETLDYVRQ RKAFGSPIAS FQHSQFLLAE LATEIDIART YLDDCLAQHL AGELTAARAA RLKWWTTELQ VRTADRCLQL HGGYGYMREY RVSQAFVDAR IQTIYGGTNE IMKTIIAKDL G
Validated NT Sequence
accctggaag ctcagaccca gggtcctggt tcgatgacct cccggcccga gtacacaccc gagcatcacc agttccggca attggtccgc gatttcgtcc accgcatcgt cgtcccggcc cacgaacccg gggaacgccg ggggcagtgg gatcgggcac tgttcaccga agccgcaagg ctggggctgc tgggattctc ggttccggaa cggttcggcg gaccgggagt gaacgacttc cgctacaacg cgatcgtcat cgacgaactg cagcgagccg gcgccgcatc cgaagccatc gccttcaccc tgcaaaacga tgtcgtgctg ccctatctca ccgacctgac cacactcgaa cagcaacaac gctggctacc cggcgtggtg accggtgaca ccgtgctcgg catcgcgatg accgaacccg gcgccggcag cgacctggcc ggcatccaca cgacagcggt acgagacggt tcgcactacg ttgtcaacgg cgccaagaca ttcatctcca acggtcaaag cggcgacctg ttcgtcatcg cggtgcgcac ctcaccggaa cgccaccagg ggctctcgct gctggtggtg gacgcaacca caccgggctt tcaccgcggc cgcagtctgg agaagatcgg cctgcacgcc caggacacca gcgaactcag tttcaccgat atgcgggttc ccgtggagaa cctgttgcag aacgagggca tggggttcta ccagctcatg cggaacctgc cgcaggagcg actggccttg gcagtgggcg cggtcgccgc cgccgaaggc atcttggccg agactctgga ctacgtgcgg cagcgcaaag ccttcggttc gccgatcgcc agctttcagc acagccagtt cctgctggcc gaactagcca ctgagatcga catcgcccga acctatctcg atgactgcct ggcccaacac ctcgccggcg agctgaccgc cgcccgcgcg gcacggctga aatggtggac caccgaactt caggtgcgta ccgcagaccg ctgcctacaa ctgcatggcg gctacggcta tatgcgggaa taccgggtgt cgcaagcatt tgtcgatgcc cgcatccaga ccatctacgg cggcaccaac gaaatcatga agaccatcat cgccaaggac ttgggttaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSRPEYTP EHHQFRQLVR DFVHRIVVPA HEPGERRGQW DRALFTEAAR LGLLGFSVPE RFGGPGVNDF RYNAIVIDEL QRAGAASEAI AFTLQNDVVL PYLTDLTTLE QQQRWLPGVV TGDTVLGIAM TEPGAGSDLA GIHTTAVRDG SHYVVNGAKT FISNGQSGDL FVIAVRTSPE RHQGLSLLVV DATTPGFHRG RSLEKIGLHA QDTSELSFTD MRVPVENLLQ NEGMGFYQLM RNLPQERLAL AVGAVAAAEG ILAETLDYVR QRKAFGSPIA SFQHSQFLLA ELATEIDIAR TYLDDCLAQH LAGELTAARA ARLKWWTTEL QVRTADRCLQ LHGGYGYMRE YRVSQAFVDA RIQTIYGGTN EIMKTIIAKD LG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacctcccgg cccgagtaca cacccgagca tcaccagttc cggcaattgg tccgcgattt cgtccaccgc atcgtcgtcc cggcccacga acccggggaa cgccgggggc agtgggatcg ggcactgttc accgaagccg caaggctggg gctgctggga ttctcggttc cggaacggtt cggcggaccg ggagtgaacg acttccgcta caacgcgatc gtcatcgacg aactgcagcg agccggcgcc gcatccgaag ccatcgcctt caccctgcaa aacgatgtcg tgctgcccta tctcaccgac ctgaccacac tcgaacagca acaacgctgg ctacccggcg tggtgaccgg tgacaccgtg ctcggcatcg cgatgaccga acccggcgcc ggcagcgacc tggccggcat ccacacgaca gcggtacgag acggttcgca ctacgttgtc aacggcgcca agacattcat ctccaacggt caaagcggcg acctgttcgt catcgcggtg cgcacctcac cggaacgcca ccaggggctc tcgctgctgg tggtggacgc aaccacaccg ggctttcacc gcggccgcag tctggagaag atcggcctgc acgcccagga caccagcgaa ctcagtttca ccgatatgcg ggttcccgtg gagaacctgt tgcagaacga gggcatgggg ttctaccagc tcatgcggaa cctgccgcag gagcgactgg ccttggcagt gggcgcggtc gccgccgccg aaggcatctt ggccgagact ctggactacg tgcggcagcg caaagccttc ggttcgccga tcgccagctt tcagcacagc cagttcctgc tggccgaact agccactgag atcgacatcg cccgaaccta tctcgatgac tgcctggccc aacacctcgc cggcgagctg accgccgccc gcgcggcacg gctgaaatgg tggaccaccg aacttcaggt gcgtaccgca gaccgctgcc tacaactgca tggcggctac ggctatatgc gggaataccg ggtgtcgcaa gcatttgtcg atgcccgcat ccagaccatc tacggcggca ccaacgaaat catgaagacc atcatcgcca aggacttggg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa