MyabA.00247.e

Probable acyl-CoA dehydrogenase FadE

CENTER ID: MyabA.00247.e
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00247.e.A1.GE30270 Full length( MyabA.00247.e ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1897
RefSeq: YP_001702600.1
UniProt: B1MNP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSELFPAYRA SWETDAHRDL RKHAAEFLRK ESTPNQERWS AQHQVDREFW NKMGDAGLLG LDLPEEYGGA GGDFGFSAVV GEELALAQDT STGWAVHSPI VAHYINTYGN EEQRARWMPG IISGDLVLAI AMTEPGTGSD LQAVRTTAIP DGDHYVINGS KTFISNGTHC DLLVIVAKTD PSQGAAGVSL IVAETKDLPG FERGRVLEKV GQHGQDTREL FFTDMRVPIA NRLGDKDGLG FYQLMEQLAR ERLIIAAVCS GMAEGALIEA IKYTKEREAF GKPLIKFQNT KFQLAELKAE VLSIKTTVDY CVQDYINGNN DPATASMAKL IAADKAVSVV DRCVQFFGGY GYMMEYPIAR AYAAARVNKI YGGTSEIMKE IISRSL
NT Sequence
ATGTCCGAAC TGTTCCCTGC CTACCGTGCG TCCTGGGAGA CCGACGCGCA CCGCGACCTG CGTAAGCATG CCGCCGAATT CCTGCGCAAG GAATCCACGC CCAACCAGGA ACGTTGGAGC GCCCAGCATC AGGTGGACCG TGAGTTCTGG AACAAGATGG GCGATGCGGG TCTGTTGGGC CTGGACCTTC CGGAGGAATA CGGTGGTGCC GGCGGTGATT TCGGATTCTC GGCGGTGGTG GGCGAAGAAC TGGCGCTCGC GCAGGACACC TCGACCGGAT GGGCCGTGCA CTCGCCGATC GTCGCGCACT ACATCAACAC CTACGGCAAT GAGGAGCAGC GTGCCCGCTG GATGCCGGGC ATCATCAGTG GCGATTTGGT CCTCGCCATC GCGATGACCG AGCCCGGCAC CGGCTCGGAC CTGCAGGCGG TGCGCACCAC CGCGATTCCC GACGGTGACC ACTACGTCAT CAACGGATCG AAAACCTTCA TCTCCAACGG AACTCACTGT GACCTGCTGG TCATTGTCGC CAAGACTGAC CCATCTCAGG GTGCCGCCGG CGTCTCACTC ATCGTCGCCG AAACCAAGGA TCTCCCCGGA TTCGAGCGCG GTCGGGTGCT GGAGAAGGTG GGTCAGCACG GTCAGGACAC GCGGGAGCTG TTCTTCACCG ACATGCGTGT GCCGATCGCA AACCGCCTCG GCGACAAGGA CGGCCTGGGC TTCTACCAGC TCATGGAACA GCTGGCCCGC GAGCGGCTGA TCATCGCCGC CGTCTGCTCC GGCATGGCCG AGGGCGCCTT GATCGAGGCC ATCAAGTACA CCAAGGAACG CGAGGCATTC GGCAAGCCGC TCATCAAATT CCAGAACACC AAATTCCAGC TGGCCGAGCT CAAGGCCGAG GTGCTGTCCA TCAAGACCAC GGTGGACTAC TGCGTTCAGG ACTACATCAA CGGGAACAAC GACCCGGCAA CGGCATCCAT GGCCAAGCTG ATCGCCGCGG ATAAGGCGGT CTCGGTGGTG GACCGTTGTG TGCAGTTCTT CGGCGGTTAC GGATACATGA TGGAGTACCC GATCGCACGG GCCTACGCCG CGGCGCGCGT CAACAAAATC TACGGCGGTA CAAGCGAAAT CATGAAGGAA ATCATCAGCC GGTCCCTG
Details for MyabA.00247.e.A1.GE30270
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMSELFPA YRASWETDAH RDLRKHAAEF LRKESTPNQE RWSAQHQVDR EFWNKMGDAG LLGLDLPEEY GGAGGDFGFS AVVGEELALA QDTSTGWAVH SPIVAHYINT YGNEEQRARW MPGIISGDLV LAIAMTEPGT GSDLQAVRTT AIPDGDHYVI NGSKTFISNG THCDLLVIVA KTDPSQGAAG VSLIVAETKD LPGFERGRVL EKVGQHGQDT RELFFTDMRV PIANRLGDKD GLGFYQLMEQ LARERLIIAA VCSGMAEGAL IEAIKYTKER EAFGKPLIKF QNTKFQLAEL KAEVLSIKTT VDYCVQDYIN GNNDPATASM AKLIAADKAV SVVDRCVQFF GGYGYMMEYP IARAYAAARV NKIYGGTSEI MKEIISRSL
Validated NT Sequence
acagggaccg gctgatgatt tccttcatga tttcgcttgt accgccgtag attttgttga cgcgcgccgc ggcgtaggcc cgtgcgatcg ggtactccat catgtatccg taaccgccga agaactgcac acaacggtcc accaccgaga ccgccttatc cgcggcgatc agcttggcca tggatgccgt tgccgggtcg ttgttcccgt tgatgtagtc ctgaacgcag tagtccaccg tggtcttgat ggacagcacc tcggccttga gctcggccag ctggaatttg gtgttctgga atttgatgag cggcttgccg aatgcctcgc gttccttggt gtacttgatg gcctcgatca aggcgccctc ggccatgccg gagcagacgg cggcgatgat cagccgctcg cgggccagct gttccatgag ctggtagaag cccaggccgt ccttgtcgcc gaggcggttt gcgatcggca cacgcatgtc ggtgaagaac agctcccgcg tgtcctgacc gtgctgaccc accttctcca gcacccgacc gcgctcgaat ccggggagat ccttggtttc ggcgacgatg agtgagacgc cggcggcacc ctgagatggg tcagtcttgg cgacaatgac cagcaggtca cagtgagttc cgttggagat gaaggttttc gatccgttga tgacgtagtg gtcaccgtcg ggaatcgcgg tggtgcgcac cgcctgcagg tccgagccgg tgccgggctc ggtcatcgcg atggcgagga ccaaatcgcc actgatgatg cccggcatcc agcgggcacg ctgctcctca ttgccgtagg tgttgatgta gtgcgcgacg atcggcgagt gcacggccca tccggtcgag gtgtcctgcg cgagcgccag ttcttcgccc accaccgccg agaatccgaa atcaccgccg gcaccaccgt attcctccgg aaggtccagg cccaacagac ccgcatcgcc catcttgttc cagaactcac ggtccacctg atgctgggcg ctccaacgtt cctggttggg cgtggattcc ttgcgcagga attcggcggc atgcttacgc aggtcgcggt gcgcgtcggt ctcccaggac gcacggtagg cagggaacag ttcggacatc gaaccaggac cctgggtctg agcttccagg gtacccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSELFPAYR ASWETDAHRD LRKHAAEFLR KESTPNQERW SAQHQVDREF WNKMGDAGLL GLDLPEEYGG AGGDFGFSAV VGEELALAQD TSTGWAVHSP IVAHYINTYG NEEQRARWMP GIISGDLVLA IAMTEPGTGS DLQAVRTTAI PDGDHYVING SKTFISNGTH CDLLVIVAKT DPSQGAAGVS LIVAETKDLP GFERGRVLEK VGQHGQDTRE LFFTDMRVPI ANRLGDKDGL GFYQLMEQLA RERLIIAAVC SGMAEGALIE AIKYTKEREA FGKPLIKFQN TKFQLAELKA EVLSIKTTVD YCVQDYINGN NDPATASMAK LIAADKAVSV VDRCVQFFGG YGYMMEYPIA RAYAAARVNK IYGGTSEIMK EIISRSL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaactg ttccctgcct accgtgcgtc ctgggagacc gacgcgcacc gcgacctgcg taagcatgcc gccgaattcc tgcgcaagga atccacgccc aaccaggaac gttggagcgc ccagcatcag gtggaccgtg agttctggaa caagatgggc gatgcgggtc tgttgggcct ggaccttccg gaggaatacg gtggtgccgg cggtgatttc ggattctcgg cggtggtggg cgaagaactg gcgctcgcgc aggacacctc gaccggatgg gccgtgcact cgccgatcgt cgcgcactac atcaacacct acggcaatga ggagcagcgt gcccgctgga tgccgggcat catcagtggc gatttggtcc tcgccatcgc gatgaccgag cccggcaccg gctcggacct gcaggcggtg cgcaccaccg cgattcccga cggtgaccac tacgtcatca acggatcgaa aaccttcatc tccaacggaa ctcactgtga cctgctggtc attgtcgcca agactgaccc atctcagggt gccgccggcg tctcactcat cgtcgccgaa accaaggatc tccccggatt cgagcgcggt cgggtgctgg agaaggtggg tcagcacggt caggacacgc gggagctgtt cttcaccgac atgcgtgtgc cgatcgcaaa ccgcctcggc gacaaggacg gcctgggctt ctaccagctc atggaacagc tggcccgcga gcggctgatc atcgccgccg tctgctccgg catggccgag ggcgccttga tcgaggccat caagtacacc aaggaacgcg aggcattcgg caagccgctc atcaaattcc agaacaccaa attccagctg gccgagctca aggccgaggt gctgtccatc aagaccacgg tggactactg cgttcaggac tacatcaacg ggaacaacga cccggcaacg gcatccatgg ccaagctgat cgccgcggat aaggcggtct cggtggtgga ccgttgtgtg cagttcttcg gcggttacgg atacatgatg gagtacccga tcgcacgggc ctacgccgcg gcgcgcgtca acaaaatcta cggcggtaca agcgaaatca tgaaggaaat catcagccgg tccctgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa