MyabA.00247.a

Probable acyl-CoA dehydrogenase

CENTER ID: MyabA.00247.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00247.a.A1.GE30266 Full length( MyabA.00247.a ) 1 384
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00247.a.A1.PS00909 Full length( MyabA.00247.a ) 1 384
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_137903
PATRIC ID: fig|36809.5.peg.3090
RefSeq: YP_001703772.1
UniProt: B1MCZ7
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGAVKFKRT IFEPEHELFR ESFKTFLDRH AEPYKEEWEK NKIVDRALWV EAGKQGFLGT DMPEEFGGGG LIDFRYNTII TEEVTKGRHT GVGFSLHNDV AGPYFRDLAN DEQKARWFPG FCSGELISAI AMTEPGTGSD LQGIKTRAVK DGDHYILNGA KTFITNGINA DLVIVVAQTD PEKGALGFSL IVVERGMEGF ERGRKLDKVG LDAQDTAELS FTDVRVPAAN LLGEEGQGFI YLMKNLPQER MSIAVMAAAA MESVLEETIA YTTERKAFGK PIKSFQNSRF LLAELATDAT VIRVMVDEFI SLLNKEELSV EQAAMAKWYS TEKQVHLIDR CLQLHGGYGF MREYPVARAY MDSRIQTIYG GTTEIMKEII GRSL
NT Sequence
ATGGCCGGCG CCGTTAAGTT CAAACGCACC ATTTTCGAAC CCGAGCACGA ACTGTTCCGC GAGTCGTTCA AGACCTTCCT GGATCGGCAC GCTGAGCCGT ACAAGGAGGA GTGGGAGAAG AACAAGATCG TCGACCGTGC ACTGTGGGTC GAGGCCGGCA AACAGGGCTT CCTGGGCACC GACATGCCCG AGGAGTTCGG TGGCGGTGGT CTTATCGATT TCCGCTACAA CACGATCATC ACCGAAGAGG TGACCAAGGG ACGCCACACC GGCGTCGGAT TCAGCCTGCA TAACGATGTC GCCGGCCCGT ACTTCCGGGA CCTGGCCAAT GACGAGCAGA AGGCCCGCTG GTTCCCCGGC TTCTGTTCCG GCGAGCTGAT TTCGGCCATC GCCATGACCG AGCCCGGCAC CGGAAGCGAT CTGCAGGGCA TCAAGACCCG CGCCGTCAAG GACGGCGATC ATTACATTCT CAATGGCGCA AAGACCTTCA TCACCAACGG CATCAACGCC GACCTGGTGA TCGTGGTCGC ACAGACCGAT CCGGAAAAGG GCGCGCTGGG TTTCTCGCTG ATCGTTGTGG AGCGCGGCAT GGAGGGCTTC GAACGCGGCC GCAAGCTCGA CAAGGTCGGT CTGGATGCTC AGGACACCGC CGAACTGTCC TTCACCGACG TCCGGGTGCC TGCGGCCAAC CTGCTGGGTG AAGAGGGTCA GGGCTTCATC TACCTGATGA AGAACCTGCC GCAGGAGCGT ATGTCGATCG CCGTCATGGC GGCCGCCGCG ATGGAGTCGG TGCTCGAAGA GACCATTGCG TACACGACCG AGCGCAAGGC ATTCGGCAAG CCCATCAAGA GCTTCCAGAA CAGTCGTTTC CTGCTCGCCG AGCTGGCCAC CGACGCAACG GTCATCCGGG TCATGGTCGA CGAGTTCATC TCGTTGCTCA ACAAAGAAGA GCTGAGTGTC GAGCAGGCCG CGATGGCCAA GTGGTACAGC ACCGAGAAGC AGGTCCACCT GATCGACCGC TGCCTGCAGC TGCACGGCGG GTACGGCTTC ATGCGCGAGT ACCCGGTGGC CCGGGCATAC ATGGACTCCC GTATCCAGAC GATCTACGGC GGCACTACCG AGATCATGAA GGAGATCATC GGGCGTTCGC TC
Details for MyabA.00247.a.A1.GE30266
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MAGAVKFKRT IFEPEHELFR ESFKTFLDRH AEPYKEEWEK NKIVDRALWV EAGKQGFLGT DMPEEFGGGG LIDFRYNTII TEEVTKGRHT GVGFSLHNDV AGPYFRDLAN DEQKARWFPG FCSGELISAI AMTEPGTGSD LQGIKTRAVK DGDHYILNGA KTFITNGINA DLVIVVAQTD PEKGALGFSL IVVERGMEGF ERGRKLDKVG LDAQDTAELS FTDVRVPAAN LLGEEGQGFI YLMKNLPQER MSIAVMAAAA MESVLEETIA YTTERKAFGK PIKSFQNSRF LLAELATDAT VIRVMVDEFI SLLNKEELSV EQAAMAKWYS TEKQVHLIDR CLQLHGGYGF MREYPVARAY MDSRIQTIYG GTTEIMKEII GRSL
Validated NT Sequence
ttattagagc gaacgcccga tgatctcctt catgatctcg gtagtgccgc cgtagatcgt ctggatacgg gagtccatgt atgcccgggc caccgggtac tcgcgcatga agccgtaccc gccgtgcagc tgcaggcagc ggtcgatcag gtggacctgc ttctcggtgc tgtaccactt ggccatcgcg gcctgctcga cactcagctc ttctttgttg agcaacgaga tgaactcgtc gaccatgacc cggatgaccg ttgcgtcggt ggccagctcg gcgagcagga aacgactgtt ctggaagctc ttgatgggct tgccgaatgc cttgcgctcg gtcgtgtacg caatggtctc ttcgagcacc gactccatcg cggcggccgc catgacggcg atcgacatac gctcctgcgg caggttcttc atcaggtaga tgaagccctg accctcttca cccagcaggt tggccgcagg cacccggacg tcggtgaagg acagttcggc ggtgtcctga gcatccagac cgaccttgtc gagcttgcgg ccgcgttcga agccctccat gccgcgctcc acaacgatca gcgagaaacc cagcgcgccc ttttccggat cggtctgtgc gaccacgatc accaggtcgg cgttgatgcc gttggtgatg aaggtctttg cgccattgag aatgtaatga tcgccgtcct tgacggcgcg ggtcttgatg ccctgcagat cgcttccggt gccgggctcg gtcatggcga tggccgaaat cagctcgccg gaacagaagc cggggaacca gcgggccttc tgctcgtcat tggccaggtc ccggaagtac gggccggcga catcgttatg caggctgaat ccgacgccgg tgtggcgtcc cttggtcacc tcttcggtga tgatcgtgtt gtagcggaaa tcgataagac caccgccacc gaactcctcg ggcatgtcgg tgcccaggaa gccctgtttg ccggcctcga cccacagtgc acggtcgacg atcttgttct tctcccactc ctccttgtac ggctcagcgt gccgatccag gaaggtcttg aacgactcgc ggaacagttc gtgctcgggt tcgaaaatgg tgcgtttgaa cttaacggcg ccggccatcg aaccaggacc ctgggtctga gcttccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGAVKFKR TIFEPEHELF RESFKTFLDR HAEPYKEEWE KNKIVDRALW VEAGKQGFLG TDMPEEFGGG GLIDFRYNTI ITEEVTKGRH TGVGFSLHND VAGPYFRDLA NDEQKARWFP GFCSGELISA IAMTEPGTGS DLQGIKTRAV KDGDHYILNG AKTFITNGIN ADLVIVVAQT DPEKGALGFS LIVVERGMEG FERGRKLDKV GLDAQDTAEL SFTDVRVPAA NLLGEEGQGF IYLMKNLPQE RMSIAVMAAA AMESVLEETI AYTTERKAFG KPIKSFQNSR FLLAELATDA TVIRVMVDEF ISLLNKEELS VEQAAMAKWY STEKQVHLID RCLQLHGGYG FMREYPVARA YMDSRIQTIY GGTTEIMKEI IGRSL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccggcgcc gttaagttca aacgcaccat tttcgaaccc gagcacgaac tgttccgcga gtcgttcaag accttcctgg atcggcacgc tgagccgtac aaggaggagt gggagaagaa caagatcgtc gaccgtgcac tgtgggtcga ggccggcaaa cagggcttcc tgggcaccga catgcccgag gagttcggtg gcggtggtct tatcgatttc cgctacaaca cgatcatcac cgaagaggtg accaagggac gccacaccgg cgtcggattc agcctgcata acgatgtcgc cggcccgtac ttccgggacc tggccaatga cgagcagaag gcccgctggt tccccggctt ctgttccggc gagctgattt cggccatcgc catgaccgag cccggcaccg gaagcgatct gcagggcatc aagacccgcg ccgtcaagga cggcgatcat tacattctca atggcgcaaa gaccttcatc accaacggca tcaacgccga cctggtgatc gtggtcgcac agaccgatcc ggaaaagggc gcgctgggtt tctcgctgat cgttgtggag cgcggcatgg agggcttcga acgcggccgc aagctcgaca aggtcggtct ggatgctcag gacaccgccg aactgtcctt caccgacgtc cgggtgcctg cggccaacct gctgggtgaa gagggtcagg gcttcatcta cctgatgaag aacctgccgc aggagcgtat gtcgatcgcc gtcatggcgg ccgccgcgat ggagtcggtg ctcgaagaga ccattgcgta cacgaccgag cgcaaggcat tcggcaagcc catcaagagc ttccagaaca gtcgtttcct gctcgccgag ctggccaccg acgcaacggt catccgggtc atggtcgacg agttcatctc gttgctcaac aaagaagagc tgagtgtcga gcaggccgcg atggccaagt ggtacagcac cgagaagcag gtccacctga tcgaccgctg cctgcagctg cacggcgggt acggcttcat gcgcgagtac ccggtggccc gggcatacat ggactcccgt atccagacga tctacggcgg cactaccgag atcatgaagg agatcatcgg gcgttcgctc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MyabA.00247.a.A1.PS00909
PURIFICATION DATe: 12/20/2010
CONCENTRATION: 76.47mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MAGAVKFKRT IFEPEHELFR ESFKTFLDRH AEPYKEEWEK NKIVDRALWV EAGKQGFLGT DMPEEFGGGG LIDFRYNTII TEEVTKGRHT GVGFSLHNDV AGPYFRDLAN DEQKARWFPG FCSGELISAI AMTEPGTGSD LQGIKTRAVK DGDHYILNGA KTFITNGINA DLVIVVAQTD PEKGALGFSL IVVERGMEGF ERGRKLDKVG LDAQDTAELS FTDVRVPAAN LLGEEGQGFI YLMKNLPQER MSIAVMAAAA MESVLEETIA YTTERKAFGK PIKSFQNSRF LLAELATDAT VIRVMVDEFI SLLNKEELSV EQAAMAKWYS TEKQVHLIDR CLQLHGGYGF MREYPVARAY MDSRIQTIYG GTTEIMKEII GRSL
Validated NT Sequence
ttattagagc gaacgcccga tgatctcctt catgatctcg gtagtgccgc cgtagatcgt ctggatacgg gagtccatgt atgcccgggc caccgggtac tcgcgcatga agccgtaccc gccgtgcagc tgcaggcagc ggtcgatcag gtggacctgc ttctcggtgc tgtaccactt ggccatcgcg gcctgctcga cactcagctc ttctttgttg agcaacgaga tgaactcgtc gaccatgacc cggatgaccg ttgcgtcggt ggccagctcg gcgagcagga aacgactgtt ctggaagctc ttgatgggct tgccgaatgc cttgcgctcg gtcgtgtacg caatggtctc ttcgagcacc gactccatcg cggcggccgc catgacggcg atcgacatac gctcctgcgg caggttcttc atcaggtaga tgaagccctg accctcttca cccagcaggt tggccgcagg cacccggacg tcggtgaagg acagttcggc ggtgtcctga gcatccagac cgaccttgtc gagcttgcgg ccgcgttcga agccctccat gccgcgctcc acaacgatca gcgagaaacc cagcgcgccc ttttccggat cggtctgtgc gaccacgatc accaggtcgg cgttgatgcc gttggtgatg aaggtctttg cgccattgag aatgtaatga tcgccgtcct tgacggcgcg ggtcttgatg ccctgcagat cgcttccggt gccgggctcg gtcatggcga tggccgaaat cagctcgccg gaacagaagc cggggaacca gcgggccttc tgctcgtcat tggccaggtc ccggaagtac gggccggcga catcgttatg caggctgaat ccgacgccgg tgtggcgtcc cttggtcacc tcttcggtga tgatcgtgtt gtagcggaaa tcgataagac caccgccacc gaactcctcg ggcatgtcgg tgcccaggaa gccctgtttg ccggcctcga cccacagtgc acggtcgacg atcttgttct tctcccactc ctccttgtac ggctcagcgt gccgatccag gaaggtcttg aacgactcgc ggaacagttc gtgctcgggt tcgaaaatgg tgcgtttgaa cttaacggcg ccggccatcg aaccaggacc ctgggtctga gcttccagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGAVKFKR TIFEPEHELF RESFKTFLDR HAEPYKEEWE KNKIVDRALW VEAGKQGFLG TDMPEEFGGG GLIDFRYNTI ITEEVTKGRH TGVGFSLHND VAGPYFRDLA NDEQKARWFP GFCSGELISA IAMTEPGTGS DLQGIKTRAV KDGDHYILNG AKTFITNGIN ADLVIVVAQT DPEKGALGFS LIVVERGMEG FERGRKLDKV GLDAQDTAEL SFTDVRVPAA NLLGEEGQGF IYLMKNLPQE RMSIAVMAAA AMESVLEETI AYTTERKAFG KPIKSFQNSR FLLAELATDA TVIRVMVDEF ISLLNKEELS VEQAAMAKWY STEKQVHLID RCLQLHGGYG FMREYPVARA YMDSRIQTIY GGTTEIMKEI IGRSL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGGCGCC GTTAAGTTCA AACGCACCAT TTTCGAACCC GAGCACGAAC TGTTCCGCGA GTCGTTCAAG ACCTTCCTGG ATCGGCACGC TGAGCCGTAC AAGGAGGAGT GGGAGAAGAA CAAGATCGTC GACCGTGCAC TGTGGGTCGA GGCCGGCAAA CAGGGCTTCC TGGGCACCGA CATGCCCGAG GAGTTCGGTG GCGGTGGTCT TATCGATTTC CGCTACAACA CGATCATCAC CGAAGAGGTG ACCAAGGGAC GCCACACCGG CGTCGGATTC AGCCTGCATA ACGATGTCGC CGGCCCGTAC TTCCGGGACC TGGCCAATGA CGAGCAGAAG GCCCGCTGGT TCCCCGGCTT CTGTTCCGGC GAGCTGATTT CGGCCATCGC CATGACCGAG CCCGGCACCG GAAGCGATCT GCAGGGCATC AAGACCCGCG CCGTCAAGGA CGGCGATCAT TACATTCTCA ATGGCGCAAA GACCTTCATC ACCAACGGCA TCAACGCCGA CCTGGTGATC GTGGTCGCAC AGACCGATCC GGAAAAGGGC GCGCTGGGTT TCTCGCTGAT CGTTGTGGAG CGCGGCATGG AGGGCTTCGA ACGCGGCCGC AAGCTCGACA AGGTCGGTCT GGATGCTCAG GACACCGCCG AACTGTCCTT CACCGACGTC CGGGTGCCTG CGGCCAACCT GCTGGGTGAA GAGGGTCAGG GCTTCATCTA CCTGATGAAG AACCTGCCGC AGGAGCGTAT GTCGATCGCC GTCATGGCGG CCGCCGCGAT GGAGTCGGTG CTCGAAGAGA CCATTGCGTA CACGACCGAG CGCAAGGCAT TCGGCAAGCC CATCAAGAGC TTCCAGAACA GTCGTTTCCT GCTCGCCGAG CTGGCCACCG ACGCAACGGT CATCCGGGTC ATGGTCGACG AGTTCATCTC GTTGCTCAAC AAAGAAGAGC TGAGTGTCGA GCAGGCCGCG ATGGCCAAGT GGTACAGCAC CGAGAAGCAG GTCCACCTGA TCGACCGCTG CCTGCAGCTG CACGGCGGGT ACGGCTTCAT GCGCGAGTAC CCGGTGGCCC GGGCATACAT GGACTCCCGT ATCCAGACGA TCTACGGCGG CACTACCGAG ATCATGAAGG AGATCATCGG GCGTTCGCTC AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA