MymaA.00247.d

Acyl-CoA dehydrogenase FadE20

CENTER ID: MymaA.00247.d
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00247.d.A1.GE29974 Full length( MymaA.00247.d ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00247.d.A1.PW29842 Full length( MymaA.00247.d ) 1 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2123
RefSeq: YP_001850294.1
UniProt: B2HL18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSAIKYQRT LFEPEHDLFR ESYRGFLERH VAPYHDEWEK AKIVDRGVWL EAGKQGFLGM AVPEEYGGGG NPDFRYNTII TEETSAGRYS GIGFGLHNDI VAPYLLALAT EEQKQRWLPK FCTGEFITAI AMTEPGTGSD LQGIKTRAVK QGDHYLLNGS KTFITNGINA DLVIVVAQTD PEKGAQGFSL LVVERGMEGF ERGRHLDKIG LDAQDTAELS FSDVQVPVEN LLGQEGMGFI YLMQNLPQER ISIAIMAAAG MESVLDQTLQ YTKERKAFGR SIGNFQNSRF VLAELATEAT MVRIMVDEFI ELHLDGKLTA EQAAMAKWYS TEKQVSLNDR CLQLHGGYGY MREYPVARAY LDSRVQTIYG GTTEIMKEII GRSLGI
NT Sequence
ATGGGCAGTG CCATCAAGTA CCAGCGCACT CTATTCGAAC CTGAGCACGA TCTATTCCGC GAGTCTTACC GCGGCTTTCT GGAGCGCCAT GTAGCGCCAT ATCACGACGA ATGGGAAAAG GCCAAGATCG TCGATCGAGG CGTGTGGCTT GAAGCCGGCA AACAAGGTTT CCTCGGCATG GCGGTACCCG AGGAGTACGG CGGCGGGGGC AACCCCGACT TCCGGTACAA CACGATCATC ACCGAGGAGA CCAGCGCCGG GCGCTATAGC GGCATCGGTT TCGGGTTGCA CAACGACATC GTGGCCCCCT ACCTGCTGGC GTTGGCCACC GAAGAGCAAA AGCAGCGTTG GCTGCCGAAG TTCTGTACCG GGGAGTTCAT TACGGCGATC GCGATGACTG AGCCGGGAAC CGGCAGTGAC CTGCAGGGCA TCAAGACCCG CGCCGTCAAG CAGGGTGATC ATTACCTGCT GAACGGTTCA AAAACCTTCA TCACCAACGG GATCAACGCG GACTTGGTCA TCGTGGTAGC TCAGACCGAT CCCGAGAAAG GCGCCCAGGG CTTTTCGCTG CTGGTGGTCG AACGCGGGAT GGAGGGCTTC GAACGGGGCC GTCATCTCGA CAAGATCGGC CTGGATGCCC AGGACACCGC GGAACTGTCC TTCTCCGACG TGCAGGTGCC GGTCGAAAAC TTGCTTGGCC AAGAGGGAAT GGGCTTCATC TACCTGATGC AGAACCTGCC CCAGGAGCGC ATCTCCATCG CCATCATGGC CGCCGCCGGG ATGGAATCGG TGCTAGACCA GACGCTGCAA TACACCAAGG AACGTAAGGC ATTTGGCAGG TCCATCGGCA ATTTTCAGAA CAGTCGCTTT GTCCTGGCTG AGCTGGCCAC CGAGGCCACC ATGGTCCGCA TCATGGTCGA CGAGTTCATC GAGTTGCACC TTGACGGCAA GTTGACGGCC GAGCAGGCCG CCATGGCCAA GTGGTACTCC ACCGAGAAGC AGGTGTCCTT GAACGACCGC TGCCTGCAGC TCCACGGGGG CTACGGGTAC ATGCGCGAAT ATCCAGTTGC CCGAGCCTAT CTGGATTCAC GGGTGCAGAC GATTTACGGT GGCACAACCG AAATCATGAA AGAGATCATC GGCCGCAGCC TGGGTATC
Details for MymaA.00247.d.A1.GE29974
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MGSAIKYQRT LFEPEHDLFR ESYRGFLERH VAPYHDEWEK AKIVDRGVWL EAGKQGFLGM AVPEEYGGGG NPDFRYNTII TEETSAGRYS GIGFGLHNDI VAPYLLALAT EEQKQRWLPK FCTGEFITAI AMTEPGTGSD LQGIKTRAVK QGDHYLLNGS KTFITNGINA DLVIVVAQTD PEKGAQGFSL LVVERGMEGF ERGRHLDKIG LDAQDTAELS FSDVQVPVEN LLGQEGMGFI YLMQNLPQER ISIAIMAAAG MESVLDQTLQ YTKERKAFGR SIGNFQNSRF VLAELATEAT MVRIMVDEFI ELHLDGKLTA EQAAMAKWYS TEKQVSLNDR CLQLHGGYGY MREYPVARAY LDSRVQTIYG GTTEIMKEII GRSLG
Validated NT Sequence
ttattaaccc aggctgcggc cgatgatctc tttcatgatt tcggttgtgc caccgtaaat cgtctgcacc cgtgaatcca gataggctcg ggcaactgga tattcgcgca tgtacccgta gcccccgtgg agctgcaggc agcggtcgtt caaggacacc tgcttctcgg tggagtacca cttggccatg gcggcctgct cggccgtcaa cttgccgtca aggtgcaact cgatgaactc gtcgaccatg atgcggacca tggtggcctc ggtggccagc tcagccagga caaagcgact gttctgaaaa ttgccgatgg acctgccaaa tgccttacgt tccttggtgt attgcagcgt ctggtctagc accgattcca tcccggcggc ggccatgatg gcgatggaga tgcgctcctg gggcaggttc tgcatcaggt agatgaagcc cattccctct tggccaagca agttttcgac cggcacctgc acgtcggaga aggacagttc cgcggtgtcc tgggcatcca ggccgatctt gtcgagatga cggccccgtt cgaagccctc catcccgcgt tcgaccacca gcagcgaaaa gccctgggcg cctttctcgg gatcggtctg agctaccacg atgaccaagt ccgcgttgat cccgttggtg atgaaggttt ttgaaccgtt cagcaggtaa tgatcaccct gcttgacggc gcgggtcttg atgccctgca ggtcactgcc ggttcccggc tcagtcatcg cgatcgccgt aatgaactcc ccggtacaga acttcggcag ccaacgctgc ttttgctctt cggtggccaa cgccagcagg tagggggcca cgatgtcgtt gtgcaacccg aaaccgatgc cgctatagcg cccggcgctg gtctcctcgg tgatgatcgt gttgtaccgg aagtcggggt tgcccccgcc gccgtactcc tcgggtaccg ccatgccgag gaaaccttgt ttgccggctt caagccacac gcctcgatcg acgatcttgg ccttttccca ttcgtcgtga tatggcgcta catggcgctc cagaaagccg cggtaagact cgcggaatag atcgtgctca ggttcgaata gagtgcgctg gtacttgatg gcactgccca tcgaaccagg accctgggtc tgagcttcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAIKYQR TLFEPEHDLF RESYRGFLER HVAPYHDEWE KAKIVDRGVW LEAGKQGFLG MAVPEEYGGG GNPDFRYNTI ITEETSAGRY SGIGFGLHND IVAPYLLALA TEEQKQRWLP KFCTGEFITA IAMTEPGTGS DLQGIKTRAV KQGDHYLLNG SKTFITNGIN ADLVIVVAQT DPEKGAQGFS LLVVERGMEG FERGRHLDKI GLDAQDTAEL SFSDVQVPVE NLLGQEGMGF IYLMQNLPQE RISIAIMAAA GMESVLDQTL QYTKERKAFG RSIGNFQNSR FVLAELATEA TMVRIMVDEF IELHLDGKLT AEQAAMAKWY STEKQVSLND RCLQLHGGYG YMREYPVARA YLDSRVQTIY GGTTEIMKEI IGRSLG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcagtgcc atcaagtacc agcgcactct attcgaacct gagcacgatc tattccgcga gtcttaccgc ggctttctgg agcgccatgt agcgccatat cacgacgaat gggaaaaggc caagatcgtc gatcgaggcg tgtggcttga agccggcaaa caaggtttcc tcggcatggc ggtacccgag gagtacggcg gcgggggcaa ccccgacttc cggtacaaca cgatcatcac cgaggagacc agcgccgggc gctatagcgg catcggtttc gggttgcaca acgacatcgt ggccccctac ctgctggcgt tggccaccga agagcaaaag cagcgttggc tgccgaagtt ctgtaccggg gagttcatta cggcgatcgc gatgactgag ccgggaaccg gcagtgacct gcagggcatc aagacccgcg ccgtcaagca gggtgatcat tacctgctga acggttcaaa aaccttcatc accaacggga tcaacgcgga cttggtcatc gtggtagctc agaccgatcc cgagaaaggc gcccagggct tttcgctgct ggtggtcgaa cgcgggatgg agggcttcga acggggccgt catctcgaca agatcggcct ggatgcccag gacaccgcgg aactgtcctt ctccgacgtg caggtgccgg tcgaaaactt gcttggccaa gagggaatgg gcttcatcta cctgatgcag aacctgcccc aggagcgcat ctccatcgcc atcatggccg ccgccgggat ggaatcggtg ctagaccaga cgctgcaata caccaaggaa cgtaaggcat ttggcaggtc catcggcaat tttcagaaca gtcgctttgt cctggctgag ctggccaccg aggccaccat ggtccgcatc atggtcgacg agttcatcga gttgcacctt gacggcaagt tgacggccga gcaggccgcc atggccaagt ggtactccac cgagaagcag gtgtccttga acgaccgctg cctgcagctc cacgggggct acgggtacat gcgcgaatat ccagttgccc gagcctatct ggattcacgg gtgcagacga tttacggtgg cacaaccgaa atcatgaaag agatcatcgg ccgcagcctg ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MymaA.00247.d.A1.PW29842
PURIFICATION DATe: 10/18/2010
CONCENTRATION: 25.01mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MGSAIKYQRT LFEPEHDLFR ESYRGFLERH VAPYHDEWEK AKIVDRGVWL EAGKQGFLGM AVPEEYGGGG NPDFRYNTII TEETSAGRYS GIGFGLHNDI VAPYLLALAT EEQKQRWLPK FCTGEFITAI AMTEPGTGSD LQGIKTRAVK QGDHYLLNGS KTFITNGINA DLVIVVAQTD PEKGAQGFSL LVVERGMEGF ERGRHLDKIG LDAQDTAELS FSDVQVPVEN LLGQEGMGFI YLMQNLPQER ISIAIMAAAG MESVLDQTLQ YTKERKAFGR SIGNFQNSRF VLAELATEAT MVRIMVDEFI ELHLDGKLTA EQAAMAKWYS TEKQVSLNDR CLQLHGGYGY MREYPVARAY LDSRVQTIYG GTTEIMKEII GRSLG
Validated NT Sequence
ttattaaccc aggctgcggc cgatgatctc tttcatgatt tcggttgtgc caccgtaaat cgtctgcacc cgtgaatcca gataggctcg ggcaactgga tattcgcgca tgtacccgta gcccccgtgg agctgcaggc agcggtcgtt caaggacacc tgcttctcgg tggagtacca cttggccatg gcggcctgct cggccgtcaa cttgccgtca aggtgcaact cgatgaactc gtcgaccatg atgcggacca tggtggcctc ggtggccagc tcagccagga caaagcgact gttctgaaaa ttgccgatgg acctgccaaa tgccttacgt tccttggtgt attgcagcgt ctggtctagc accgattcca tcccggcggc ggccatgatg gcgatggaga tgcgctcctg gggcaggttc tgcatcaggt agatgaagcc cattccctct tggccaagca agttttcgac cggcacctgc acgtcggaga aggacagttc cgcggtgtcc tgggcatcca ggccgatctt gtcgagatga cggccccgtt cgaagccctc catcccgcgt tcgaccacca gcagcgaaaa gccctgggcg cctttctcgg gatcggtctg agctaccacg atgaccaagt ccgcgttgat cccgttggtg atgaaggttt ttgaaccgtt cagcaggtaa tgatcaccct gcttgacggc gcgggtcttg atgccctgca ggtcactgcc ggttcccggc tcagtcatcg cgatcgccgt aatgaactcc ccggtacaga acttcggcag ccaacgctgc ttttgctctt cggtggccaa cgccagcagg tagggggcca cgatgtcgtt gtgcaacccg aaaccgatgc cgctatagcg cccggcgctg gtctcctcgg tgatgatcgt gttgtaccgg aagtcggggt tgcccccgcc gccgtactcc tcgggtaccg ccatgccgag gaaaccttgt ttgccggctt caagccacac gcctcgatcg acgatcttgg ccttttccca ttcgtcgtga tatggcgcta catggcgctc cagaaagccg cggtaagact cgcggaatag atcgtgctca ggttcgaata gagtgcgctg gtacttgatg gcactgccca tcgaaccagg accctgggtc tgagcttcc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAIKYQR TLFEPEHDLF RESYRGFLER HVAPYHDEWE KAKIVDRGVW LEAGKQGFLG MAVPEEYGGG GNPDFRYNTI ITEETSAGRY SGIGFGLHND IVAPYLLALA TEEQKQRWLP KFCTGEFITA IAMTEPGTGS DLQGIKTRAV KQGDHYLLNG SKTFITNGIN ADLVIVVAQT DPEKGAQGFS LLVVERGMEG FERGRHLDKI GLDAQDTAEL SFSDVQVPVE NLLGQEGMGF IYLMQNLPQE RISIAIMAAA GMESVLDQTL QYTKERKAFG RSIGNFQNSR FVLAELATEA TMVRIMVDEF IELHLDGKLT AEQAAMAKWY STEKQVSLND RCLQLHGGYG YMREYPVARA YLDSRVQTIY GGTTEIMKEI IGRSLG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCAGTGCC ATCAAGTACC AGCGCACTCT ATTCGAACCT GAGCACGATC TATTCCGCGA GTCTTACCGC GGCTTTCTGG AGCGCCATGT AGCGCCATAT CACGACGAAT GGGAAAAGGC CAAGATCGTC GATCGAGGCG TGTGGCTTGA AGCCGGCAAA CAAGGTTTCC TCGGCATGGC GGTACCCGAG GAGTACGGCG GCGGGGGCAA CCCCGACTTC CGGTACAACA CGATCATCAC CGAGGAGACC AGCGCCGGGC GCTATAGCGG CATCGGTTTC GGGTTGCACA ACGACATCGT GGCCCCCTAC CTGCTGGCGT TGGCCACCGA AGAGCAAAAG CAGCGTTGGC TGCCGAAGTT CTGTACCGGG GAGTTCATTA CGGCGATCGC GATGACTGAG CCGGGAACCG GCAGTGACCT GCAGGGCATC AAGACCCGCG CCGTCAAGCA GGGTGATCAT TACCTGCTGA ACGGTTCAAA AACCTTCATC ACCAACGGGA TCAACGCGGA CTTGGTCATC GTGGTAGCTC AGACCGATCC CGAGAAAGGC GCCCAGGGCT TTTCGCTGCT GGTGGTCGAA CGCGGGATGG AGGGCTTCGA ACGGGGCCGT CATCTCGACA AGATCGGCCT GGATGCCCAG GACACCGCGG AACTGTCCTT CTCCGACGTG CAGGTGCCGG TCGAAAACTT GCTTGGCCAA GAGGGAATGG GCTTCATCTA CCTGATGCAG AACCTGCCCC AGGAGCGCAT CTCCATCGCC ATCATGGCCG CCGCCGGGAT GGAATCGGTG CTAGACCAGA CGCTGCAATA CACCAAGGAA CGTAAGGCAT TTGGCAGGTC CATCGGCAAT TTTCAGAACA GTCGCTTTGT CCTGGCTGAG CTGGCCACCG AGGCCACCAT GGTCCGCATC ATGGTCGACG AGTTCATCGA GTTGCACCTT GACGGCAAGT TGACGGCCGA GCAGGCCGCC ATGGCCAAGT GGTACTCCAC CGAGAAGCAG GTGTCCTTGA ACGACCGCTG CCTGCAGCTC CACGGGGGCT ACGGGTACAT GCGCGAATAT CCAGTTGCCC GAGCCTATCT GGATTCACGG GTGCAGACGA TTTACGGTGG CACAACCGAA ATCATGAAAG AGATCATCGG CCGCAGCCTG GGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA