SSGCID: Target Page

MymaA.00247.c

Acyl-CoA dehydrogenase

CENTER ID:	MymaA.00247.c
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00247.c.A1.GE29973	Full length( MymaA.00247.c )		1	381

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.3349
RefSeq:	YP_001851421.1
UniProt:	B2HG02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTASISPWMS AELLELRDLA AKFFSTELAP HAQRFADQHQ VDRELWHKAG ELGLLCMSIP EEYGGGGGTF AHEAVVLEEQ ARVGDSSWGA GLHSGIVAHY ILQYAAEELR RQWLPKMASG EMIGAIAMTE PGTGSDLQSV KTKAILDGDE YVITGAKTFI TNGQQADLII VVAKTDPSQG AAGISLIVAE ADRPGFRRGK VLDKIGQRGQ DTSELFFDDV RVPRSHLLGE TEGQGFIQLM TQLPQERLIV AVGAVAAMEL AVEQTLKYTR EREAFGRPVF GFQNTKFILA EAATETRIAR VFLDYCIDLH LAGQLDVQTV AMAKWWTTER AMKVLDDCLQ LHGGYGYMTE YPISRLWVDQ RVQKIYAGTN EVMKEIISRS L

NT Sequence

GTGACAGCAT CAATTTCTCC CTGGATGAGC GCCGAATTGC TCGAGCTTCG TGACCTTGCC GCGAAGTTCT TCTCGACCGA ATTGGCCCCA CATGCGCAAC GTTTCGCAGA CCAGCACCAA GTTGACCGAG AATTGTGGCA CAAAGCCGGC GAACTAGGCC TGCTCTGCAT GTCCATACCC GAAGAATACG GCGGGGGAGG CGGCACATTC GCGCACGAAG CCGTCGTACT CGAAGAGCAA GCCCGAGTCG GGGACAGCTC ATGGGGCGCA GGGCTGCACA GCGGAATCGT CGCGCACTAC ATCCTCCAGT ATGCGGCCGA AGAACTACGG AGGCAGTGGC TGCCCAAGAT GGCCTCAGGC GAAATGATCG GTGCTATCGC CATGACGGAG CCAGGGACCG GTTCTGATCT GCAGAGTGTC AAGACCAAAG CAATTCTTGA CGGTGACGAG TACGTGATCA CCGGCGCAAA GACCTTCATC ACCAACGGTC AGCAAGCCGA CCTGATCATC GTCGTCGCCA AGACAGATCC AAGTCAGGGC GCCGCCGGCA TCTCGCTGAT CGTCGCCGAA GCCGATCGAC CCGGATTCCG GCGCGGCAAG GTCCTCGACA AAATCGGCCA ACGCGGACAA GACACCTCCG AGTTGTTCTT CGACGACGTA AGAGTGCCCC GCTCGCACCT CCTGGGCGAG ACCGAGGGTC AGGGTTTCAT TCAGCTGATG ACGCAGCTAC CTCAAGAGCG GCTCATCGTC GCGGTTGGGG CGGTCGCGGC GATGGAACTT GCCGTGGAGC AGACACTCAA ATACACCCGT GAGCGGGAAG CGTTCGGACG GCCCGTCTTC GGCTTCCAGA ACACGAAATT CATCTTGGCC GAAGCTGCAA CCGAAACGCG CATCGCACGA GTATTTTTGG ACTACTGCAT CGACCTACAC CTCGCGGGCC AACTCGACGT CCAGACCGTC GCCATGGCGA AATGGTGGAC CACTGAGCGA GCGATGAAGG TGCTCGATGA CTGTTTGCAG CTCCACGGCG GATATGGGTA CATGACCGAG TACCCCATCT CGAGATTGTG GGTGGACCAG CGCGTGCAGA AAATCTACGC CGGCACAAAT GAGGTGATGA AGGAAATCAT CTCGCGTTCC CTA

Details for MymaA.00247.c.A1.GE29973

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/20/2010
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	94

Validated AA Sequence

MVTASISPWM SAELLELRDL AAKFFSTELA PHAQRFADQH QVDRELWHKA GELGLLCMSI PEEYGGGGGT FAHEAVVLEX QARVGDSSWG AGLHSGIVAH YILQYAAEEL RRQWLPKMAS GEMIGAIAMT XPGTGSDLQS VKTKAILDGD EYVITGAKTF ITNGQQADLI IVVAKTDPSQ GAAGISLIVA EADRPGFRRG KVLDKIGQRG QDTSELFFDD VRVPRSHLLG ETEGQGFIQL MTQLPQERLI VAVGAVAAME LAVEQTLKYT REREAFGRPV FGFQNTKFIL AEAATETRIA RVFLDYCIDL HLAGQLDVQT VAMAKWWTTE RAMKVLDDCL QLHGGYGYMT EYPISRLWVD QRVQKIYAGT NEVMKEIISR S

Validated NT Sequence

ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tgacagcatc aatttctccc tggatgagcg ccgaattgct cgagcttcgt gaccttgccg cgaagttctt ctcgaccgaa ttggccccac atgcgcaacg tttcgcagac cagcaccaag ttgaccgaga attgtggcac aaagccggcg aactaggcct gctctgcatg tccatacccg aagaatacgg cgggggaggc ggcacattcg cgcacgaagc cgtcgtactc gaanagcaag cccgagtcgg ggacagctca tggggcgcag ggctgcacag cggaatcgtc gcgcactaca tcctccagta tgcggccgaa gaactacgga ggcagtggct gcccaagatg gcntcaggcg aaatgatcgg tgctatcgcc atgacgnagc cagggaccgg ttctgatctg cagagtgtca agaccaaagc aattcttgac ggtgacgagt acgtgatcac cggcgcaaag accttcatca ccaacggtca gcaagccgac ctgatcatcg tcgtcgccaa gacagatcca agtcagggcg ccgccggcat ctcgctgatc gtcgccgaag ccgatcgacc cggattccgg cgcggcaagg tcctcgacaa aatcggccaa cgcggacaag acacctccga gttgttcttc gacgacgtaa gagtgccccg ctcgcacctc ctgggcgaga ccgagggtca gggtttcatt cagctgatga cgcagctacc tcaagagcgg ctcatcgtcg cggttggggc ggtcgcggcg atggaacttg ccgtggagca gacactcaaa tacacccgtg agcgggaagc gttcggacgg cccgtcttcg gcttccagaa cacgaaattc atcttggccg aagctgcaac cgaaacgcgc atcgcacgag tatttttgga ctactgcatc gacctacacc tcgcgggcca actcgacgtc cagaccgtcg ccatggcgaa atggtggacc actgagcgag cgatgaaggt gctcgatgac tgtttgcagc tccacggcgg atatgggtac atgaccgagt accccatctc gagattgtgg gtggaccagc gcgtgcagaa aatctacgcc ggcacaaatg aggtgatgaa ggaaatcatc tcgcgttcct

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTASISPW MSAELLELRD LAAKFFSTEL APHAQRFADQ HQVDRELWHK AGELGLLCMS IPEEYGGGGG TFAHEAVVLE EQARVGDSSW GAGLHSGIVA HYILQYAAEE LRRQWLPKMA SGEMIGAIAM TEPGTGSDLQ SVKTKAILDG DEYVITGAKT FITNGQQADL IIVVAKTDPS QGAAGISLIV AEADRPGFRR GKVLDKIGQR GQDTSELFFD DVRVPRSHLL GETEGQGFIQ LMTQLPQERL IVAVGAVAAM ELAVEQTLKY TREREAFGRP VFGFQNTKFI LAEAATETRI ARVFLDYCID LHLAGQLDVQ TVAMAKWWTT ERAMKVLDDC LQLHGGYGYM TEYPISRLWV DQRVQKIYAG TNEVMKEIIS RS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacagca tcaatttctc cctggatgag cgccgaattg ctcgagcttc gtgaccttgc cgcgaagttc ttctcgaccg aattggcccc acatgcgcaa cgtttcgcag accagcacca agttgaccga gaattgtggc acaaagccgg cgaactaggc ctgctctgca tgtccatacc cgaagaatac ggcgggggag gcggcacatt cgcgcacgaa gccgtcgtac tcgaagagca agcccgagtc ggggacagct catggggcgc agggctgcac agcggaatcg tcgcgcacta catcctccag tatgcggccg aagaactacg gaggcagtgg ctgcccaaga tggcctcagg cgaaatgatc ggtgctatcg ccatgacgga gccagggacc ggttctgatc tgcagagtgt caagaccaaa gcaattcttg acggtgacga gtacgtgatc accggcgcaa agaccttcat caccaacggt cagcaagccg acctgatcat cgtcgtcgcc aagacagatc caagtcaggg cgccgccggc atctcgctga tcgtcgccga agccgatcga cccggattcc ggcgcggcaa ggtcctcgac aaaatcggcc aacgcggaca agacacctcc gagttgttct tcgacgacgt aagagtgccc cgctcgcacc tcctgggcga gaccgagggt cagggtttca ttcagctgat gacgcagcta cctcaagagc ggctcatcgt cgcggttggg gcggtcgcgg cgatggaact tgccgtggag cagacactca aatacacccg tgagcgggaa gcgttcggac ggcccgtctt cggcttccag aacacgaaat tcatcttggc cgaagctgca accgaaacgc gcatcgcacg agtatttttg gactactgca tcgacctaca cctcgcgggc caactcgacg tccagaccgt cgccatggcg aaatggtgga ccactgagcg agcgatgaag gtgctcgatg actgtttgca gctccacggc ggatatgggt acatgaccga gtaccccatc tcgagattgt gggtggacca gcgcgtgcag aaaatctacg ccggcacaaa tgaggtgatg aaggaaatca tctcgcgttc caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa