MyulA.00247.b

Acyl-CoA dehydrogenase FadE20

CENTER ID: MyulA.00247.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00247.b.A1.GE29664 Full length( MyulA.00247.b ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3920
RefSeq: YP_907018.1
UniProt: A0PT78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSAIKYQRT LFEPEHDLFR ESYRGFLERH VALYHDEWEK AKIVDRGVWL EAGKQGFLGM AVPEEYGGGG NPDFRYNTII TEETSAGRYS GIGFGLHNEI VAPYLLALAT EEQKQRRLPK FCTGEFITAI AMTEPGTGSD LQGIKTRAVK QGDHYLLNGS KTFITNGINA DLVIVVAQTD PEKGAQGFSL LVVERGMEGF ERGRHLDKIG LDAQDTAELS FSDVQVPVEN LLGQEGMGFI YLMQNLPQER ISIAIMAAAG MESVLDQTLQ YTKERKAFGR SIGNFQNSRF VLAELATEAT MVRIMVDESI ELHLDGKLTA EQAAMAKWYS TEKQVSLNDR CLQLHGGYGY MREYPVARAY LDSRVQTIYG GTTEIMKEII GRSLGI
NT Sequence
ATGGGCAGTG CCATCAAGTA CCAGCGCACT TTATTCGAAC CTGAGCACGA TCTATTCCGC GAGTCTTACC GCGGCTTTCT GGAGCGCCAT GTAGCGCTAT ATCACGACGA ATGGGAAAAG GCCAAGATCG TCGATCGAGG CGTGTGGCTT GAAGCCGGCA AACAAGGTTT CCTCGGCATG GCGGTACCCG AGGAGTACGG CGGCGGGGGC AACCCCGACT TCCGGTACAA CACGATCATC ACCGAGGAGA CCAGCGCCGG GCGCTATAGC GGCATCGGTT TCGGGTTGCA CAACGAGATC GTGGCCCCCT ACCTGCTGGC GTTGGCAACC GAAGAGCAAA AGCAGCGTCG GCTGCCGAAG TTCTGTACCG GGGAGTTCAT TACGGCGATC GCGATGACTG AGCCGGGAAC CGGCAGTGAC CTGCAGGGCA TCAAGACCCG CGCCGTCAAG CAGGGCGATC ATTACCTGCT GAACGGTTCA AAAACCTTCA TCACCAACGG GATCAACGCG GACTTGGTCA TCGTGGTAGC TCAGACCGAT CCCGAGAAAG GCGCCCAGGG CTTTTCGCTG CTGGTGGTCG AACGCGGGAT GGAGGGCTTC GAACGGGGCC GTCATCTCGA CAAGATCGGC CTGGATGCCC AGGACACCGC GGAACTGTCC TTCTCCGACG TGCAGGTGCC GGTCGAAAAC TTGCTTGGCC AAGAGGGAAT GGGCTTCATC TACCTGATGC AGAACCTGCC CCAGGAGCGC ATCTCCATCG CCATCATGGC CGCCGCCGGG ATGGAATCGG TGCTAGACCA GACGCTGCAA TACACCAAGG AACGTAAGGC ATTTGGCAGG TCCATCGGCA ATTTTCAGAA CAGTCGCTTT GTCCTGGCTG AGCTGGCCAC CGAGGCCACC ATGGTCCGCA TCATGGTCGA CGAGTCCATC GAGTTGCACC TTGACGGCAA GTTGACGGCC GAGCAGGCCG CCATGGCCAA GTGGTACTCC ACCGAGAAGC AGGTGTCCTT GAACGACCGG TGCCTGCAGC TCCACGGGGG CTACGGGTAC ATGCGCGAAT ATCCAGTTGC CCGAGCCTAT CTGGATTCAC GGGTGCAGAC GATTTACGGT GGCACAACCG AAATCATGAA AGAGATCATC GGCCGCAGCC TGGGTATC
Details for MyulA.00247.b.A1.GE29664
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 45kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 62
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAIKYQR TLFEPEHDLF RESYRGFLER HVALYHDEWE KAKIVDRGVW LEAGKQGFLG MAVPEEYGGG GNPDFRYNTI ITEETSAGRY SGIGFGLHNE IVAPYLLALA TEEQKQRXXX KFCTGEFITA IAMTEPGTGS DLQGIKTRAV KQGDHYLLNG SKTFITNGIN ADLVIVVAQT DPEKGAQGFS LLXVERGMEG FERGRHLDXS AXXPRTPRNC XXPTCXCRSK LAWXXGMGFI YLMXXXPXXX XXXXSXXXXX XXAXXXXAXX XXRXXXXXPX XXXXXXXXGX XXXPXXXXXX XXXXXXXRXX XXXXXXXXXX XXXGXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
tttgtttnnc tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggcag tgccatcaag taccagcgca ctttattcga acctgagcac gatctattcc gcgagtctta ccgcggcttt ctggagcgcc atgtagcgct atatcacgac gaatgggaaa aggccaagat cgtcgatcga ggcgtgtggc ttgaagccgg caaacaaggt ttcctcggca tggcggtacc cgaggagtac ggcggcgggg gcaaccccga cttccggtac aacacgatca tcaccgagga gaccagcgcc gggcgctata gcggcatcgg tttcgggttg cacaacgaga tcgtggcccc ctacctgctg gcgttggcaa ccgaagagca aaagcagcgt cnnnnnncga agttctgtac cggggagttc attacggcga tcgcgatgac tgagccggga accggcagtg acctgcaggg catcaagacc cgcgccgtca agcagggcga tcattacctg ctgaacggtt caaaaacctt catcaccaac gggatcaacg cggacttggt catcgtggta gctcagaccg atcccgagaa aggcgcccag ggcttttcgc tgctggnggt cgaacgcggg atggagggct tcgaacgggg ccgtcatctc gacanatcgg ccnggntgcc caggacaccg cgnaactgtc ntnnnccnac gtgcagntgc cggtcgaaac ttgcttggnn aanaggnatg ggcttcatct acctgatgca nanctgnccc ngnannnnca tnncatnnnc ntcannnnnc cncngnngna ncgnngctnn nnnnnnnnct gcannnnnnn nngnncgnaa ngnattngna ngnccnnnnn ntttnnnaan antnnnttnn ncnggntnnn nnnnnnnncc nnnnncnnnn nnnnnnnnna nnnnncntnn nnnnncntnn cgnnnnnacg nnnnnnnnnn nnnnnnnnnn nngnnnnnnn nnnnnnnngg nnntnannnn nnntgnnnnn nnngnnnnnn nnnnnnnnnn nnnnnnnnnn cntnngnntn nnngnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSAIKYQR TLFEPEHDLF RESYRGFLER HVALYHDEWE KAKIVDRGVW LEAGKQGFLG MAVPEEYGGG GNPDFRYNTI ITEETSAGRY SGIGFGLHNE IVAPYLLALA TEEQKQRRLP KFCTGEFITA IAMTEPGTGS DLQGIKTRAV KQGDHYLLNG SKTFITNGIN ADLVIVVAQT DPEKGAQGFS LLVVERGMEG FERGRHLDKI GLDAQDTAEL SFSDVQVPVE NLLGQEGMGF IYLMQNLPQE RISIAIMAAA GMESVLDQTL QYTKERKAFG RSIGNFQNSR FVLAELATEA TMVRIMVDES IELHLDGKLT AEQAAMAKWY STEKQVSLND RCLQLHGGYG YMREYPVARA YLDSRVQTIY GGTTEIMKEI IGRSLGI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcagtgcc atcaagtacc agcgcacttt attcgaacct gagcacgatc tattccgcga gtcttaccgc ggctttctgg agcgccatgt agcgctatat cacgacgaat gggaaaaggc caagatcgtc gatcgaggcg tgtggcttga agccggcaaa caaggtttcc tcggcatggc ggtacccgag gagtacggcg gcgggggcaa ccccgacttc cggtacaaca cgatcatcac cgaggagacc agcgccgggc gctatagcgg catcggtttc gggttgcaca acgagatcgt ggccccctac ctgctggcgt tggcaaccga agagcaaaag cagcgtcggc tgccgaagtt ctgtaccggg gagttcatta cggcgatcgc gatgactgag ccgggaaccg gcagtgacct gcagggcatc aagacccgcg ccgtcaagca gggcgatcat tacctgctga acggttcaaa aaccttcatc accaacggga tcaacgcgga cttggtcatc gtggtagctc agaccgatcc cgagaaaggc gcccagggct tttcgctgct ggtggtcgaa cgcgggatgg agggcttcga acggggccgt catctcgaca agatcggcct ggatgcccag gacaccgcgg aactgtcctt ctccgacgtg caggtgccgg tcgaaaactt gcttggccaa gagggaatgg gcttcatcta cctgatgcag aacctgcccc aggagcgcat ctccatcgcc atcatggccg ccgccgggat ggaatcggtg ctagaccaga cgctgcaata caccaaggaa cgtaaggcat ttggcaggtc catcggcaat tttcagaaca gtcgctttgt cctggctgag ctggccaccg aggccaccat ggtccgcatc atggtcgacg agtccatcga gttgcacctt gacggcaagt tgacggccga gcaggccgcc atggccaagt ggtactccac cgagaagcag gtgtccttga acgaccggtg cctgcagctc cacgggggct acgggtacat gcgcgaatat ccagttgccc gagcctatct ggattcacgg gtgcagacga tttacggtgg cacaaccgaa atcatgaaag agatcatcgg ccgcagcctg ggtatcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa