MyabA.00247.b

Probable acyl-CoA dehydrogenase (FadE)

CENTER ID: MyabA.00247.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00247.b.A1.GE30267 Full length( MyabA.00247.b ) 1 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00247.b.A1.PS00930 Full length( MyabA.00247.b ) 1 381

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.96
RefSeq: YP_001700819.1
UniProt: B1MEA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRTIYTEDH EAFRESVKAF VQRHVDPRAE TFIEQRYIDR ELWEEAGKQG YLGLDVPEEF GGSSAGDYRY NAVLQEELAR SSAALASSMS IHFDIVAPYL VELTTDEQKQ RWLPKFCSGE MITAIGMTEP SGGSDLAALK TTAVKDGDDW VINGSKTFIT NGARADLVVV AARTAPELKA KGITLFAVEE GMPGFERGRK LDKVGQPEAD TAELFFTDVR VPAENVIGEV NQGFISMMQR LPQERMGCAV ANIAHAVAVL EETVEYAKER KAFGQQIGSL QYNKFLIAEL VTKLEAAQVY VDQAVLAHSK GELTAIDAAK AKWWSSQVQN EVIDACVQLH GGYGYMKEYR VARAWMDARV TKIWAGSNEI MKELIGRDLG F
NT Sequence
ATGCAACGGA CCATCTACAC CGAGGACCAC GAGGCATTCC GCGAGTCCGT GAAGGCGTTT GTGCAACGCC ACGTCGACCC GCGCGCCGAG ACATTCATCG AGCAGCGCTA CATTGACCGC GAGCTCTGGG AAGAGGCCGG TAAGCAGGGT TACCTGGGCC TCGACGTCCC CGAGGAGTTC GGCGGCAGCA GCGCCGGCGA CTACCGCTAC AACGCGGTAC TGCAGGAGGA ACTGGCCCGC TCCAGCGCGG CCCTGGCCTC CTCGATGTCC ATTCACTTCG ACATCGTCGC CCCGTACCTC GTCGAGCTGA CCACCGACGA GCAGAAGCAA CGCTGGCTGC CCAAGTTCTG TAGCGGCGAG ATGATCACCG CGATCGGCAT GACCGAGCCC TCGGGTGGCT CCGATCTGGC GGCCCTCAAG ACAACGGCCG TCAAGGACGG CGACGACTGG GTCATCAACG GCTCCAAGAC GTTCATCACC AACGGTGCGC GAGCCGATCT CGTCGTCGTC GCGGCACGCA CCGCGCCCGA GCTGAAGGCC AAGGGCATCA CCCTGTTCGC GGTCGAGGAG GGCATGCCCG GCTTCGAGCG CGGCCGCAAG CTGGACAAAG TGGGCCAGCC CGAGGCCGAT ACCGCCGAGT TGTTCTTCAC CGATGTGCGG GTGCCCGCCG AGAACGTCAT CGGCGAGGTC AACCAGGGAT TCATCTCGAT GATGCAGCGC CTGCCTCAGG AGCGGATGGG CTGTGCGGTC GCCAACATCG CACACGCGGT CGCCGTGCTG GAGGAGACCG TCGAATACGC CAAGGAGCGC AAGGCGTTCG GTCAGCAGAT CGGCAGCCTG CAGTACAACA AGTTCCTCAT CGCCGAGCTC GTCACCAAGC TGGAAGCGGC ACAGGTGTAC GTCGATCAGG CAGTACTGGC CCACAGCAAG GGCGAGCTCA CCGCGATTGA CGCGGCCAAG GCCAAGTGGT GGTCGTCGCA GGTCCAGAAC GAGGTCATCG ACGCCTGCGT ACAGCTACAC GGCGGGTACG GCTACATGAA GGAGTACCGG GTCGCACGCG CTTGGATGGA CGCGCGCGTC ACCAAGATCT GGGCAGGCTC CAACGAGATC ATGAAGGAAC TCATCGGCCG CGACCTGGGC TTC
Details for MyabA.00247.b.A1.GE30267
HARVESTED ON: 8/31/2010
SEQUENCED ON: 9/7/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MQRTIYTEDH EAFRESVKAF VQRHVDPRAE TFIEQRYIDR ELWEEAGKQG YLGLDVPEEF GGSSAGDYRY NAVLQEELAR SSAALASSMS IHFDIVAPYL VELTTDEQKQ RWLPKFCSGE MITAIGMTEP SGGSDLAALK TTAVKDGDDW VINGSKTFIT NGARADLVVV AARTAPELKA KGITLFAVEE GMPGFERGRK LDKVGQPEAD TAELFFTDVR VPAENVIGEV NQGFISMMQR LPQERMGCAV ANIAHAVAVL EETVEYAKER KAFGQQIGSL QYNKFLIAEL VTKLEAAQVY VDQAVLAHSK GELTAIDAAK AKWWSSQVQN EVIDACVQLH GGYGYMKEYR VARAWMDARV TKIWAGSNEI MKELIGRDLG F
Validated NT Sequence
ttattagaag cccaggtcgc ggccgatgag ttccttcatg atctcgttgg agcctgccca gatcttggtg acgcgcgcgt ccatccaagc gcgtgcgacc cggtactcct tcatgtagcc gtacccgccg tgtagctgta cgcaggcgtc gatgacctcg ttctggacct gcgacgacca ccacttggcc ttggccgcgt caatcgcggt gagctcgccc ttgctgtggg ccagtactgc ctgatcgacg tacacctgtg ccgcttccag cttggtgacg agctcggcga tgaggaactt gttgtactgc aggctgccga tctgctgacc gaacgccttg cgctccttgg cgtattcgac ggtctcctcc agcacggcga ccgcgtgtgc gatgttggcg accgcacagc ccatccgctc ctgaggcagg cgctgcatca tcgagatgaa tccctggttg acctcgccga tgacgttctc ggcgggcacc cgcacatcgg tgaagaacaa ctcggcggta tcggcctcgg gctggcccac tttgtccagc ttgcggccgc gctcgaagcc gggcatgccc tcctcgaccg cgaacagggt gatgcccttg gccttcagct cgggcgcggt gcgtgccgcg acgacgacga gatcggctcg cgcaccgttg gtgatgaacg tcttggagcc gttgatgacc cagtcgtcgc cgtccttgac ggccgttgtc ttgagggccg ccagatcgga gccacccgag ggctcggtca tgccgatcgc ggtgatcatc tcgccgctac agaacttggg cagccagcgt tgcttctgct cgtcggtggt cagctcgacg aggtacgggg cgacgatgtc gaagtgaatg gacatcgagg aggccagggc cgcgctggag cgggccagtt cctcctgcag taccgcgttg tagcggtagt cgccggcgct gctgccgccg aactcctcgg ggacgtcgag gcccaggtaa ccctgcttac cggcctcttc ccagagctcg cggtcaatgt agcgctgctc gatgaatgtc tcggcgcgcg ggtcgacgtg gcgttgcaca aacgccttca cggactcgcg gaatgcctcg tggtcctcgg tgtagatggt ccgttgcatc gaaccaggac cctgggtctg agcttccagg gtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRTIYTED HEAFRESVKA FVQRHVDPRA ETFIEQRYID RELWEEAGKQ GYLGLDVPEE FGGSSAGDYR YNAVLQEELA RSSAALASSM SIHFDIVAPY LVELTTDEQK QRWLPKFCSG EMITAIGMTE PSGGSDLAAL KTTAVKDGDD WVINGSKTFI TNGARADLVV VAARTAPELK AKGITLFAVE EGMPGFERGR KLDKVGQPEA DTAELFFTDV RVPAENVIGE VNQGFISMMQ RLPQERMGCA VANIAHAVAV LEETVEYAKE RKAFGQQIGS LQYNKFLIAE LVTKLEAAQV YVDQAVLAHS KGELTAIDAA KAKWWSSQVQ NEVIDACVQL HGGYGYMKEY RVARAWMDAR VTKIWAGSNE IMKELIGRDL GF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaacggacc atctacaccg aggaccacga ggcattccgc gagtccgtga aggcgtttgt gcaacgccac gtcgacccgc gcgccgagac attcatcgag cagcgctaca ttgaccgcga gctctgggaa gaggccggta agcagggtta cctgggcctc gacgtccccg aggagttcgg cggcagcagc gccggcgact accgctacaa cgcggtactg caggaggaac tggcccgctc cagcgcggcc ctggcctcct cgatgtccat tcacttcgac atcgtcgccc cgtacctcgt cgagctgacc accgacgagc agaagcaacg ctggctgccc aagttctgta gcggcgagat gatcaccgcg atcggcatga ccgagccctc gggtggctcc gatctggcgg ccctcaagac aacggccgtc aaggacggcg acgactgggt catcaacggc tccaagacgt tcatcaccaa cggtgcgcga gccgatctcg tcgtcgtcgc ggcacgcacc gcgcccgagc tgaaggccaa gggcatcacc ctgttcgcgg tcgaggaggg catgcccggc ttcgagcgcg gccgcaagct ggacaaagtg ggccagcccg aggccgatac cgccgagttg ttcttcaccg atgtgcgggt gcccgccgag aacgtcatcg gcgaggtcaa ccagggattc atctcgatga tgcagcgcct gcctcaggag cggatgggct gtgcggtcgc caacatcgca cacgcggtcg ccgtgctgga ggagaccgtc gaatacgcca aggagcgcaa ggcgttcggt cagcagatcg gcagcctgca gtacaacaag ttcctcatcg ccgagctcgt caccaagctg gaagcggcac aggtgtacgt cgatcaggca gtactggccc acagcaaggg cgagctcacc gcgattgacg cggccaaggc caagtggtgg tcgtcgcagg tccagaacga ggtcatcgac gcctgcgtac agctacacgg cgggtacggc tacatgaagg agtaccgggt cgcacgcgct tggatggacg cgcgcgtcac caagatctgg gcaggctcca acgagatcat gaaggaactc atcggccgcg acctgggctt caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MyabA.00247.b.A1.PS00930
PURIFICATION DATe: 1/5/2011
CONCENTRATION: 53.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MQRTIYTEDH EAFRESVKAF VQRHVDPRAE TFIEQRYIDR ELWEEAGKQG YLGLDVPEEF GGSSAGDYRY NAVLQEELAR SSAALASSMS IHFDIVAPYL VELTTDEQKQ RWLPKFCSGE MITAIGMTEP SGGSDLAALK TTAVKDGDDW VINGSKTFIT NGARADLVVV AARTAPELKA KGITLFAVEE GMPGFERGRK LDKVGQPEAD TAELFFTDVR VPAENVIGEV NQGFISMMQR LPQERMGCAV ANIAHAVAVL EETVEYAKER KAFGQQIGSL QYNKFLIAEL VTKLEAAQVY VDQAVLAHSK GELTAIDAAK AKWWSSQVQN EVIDACVQLH GGYGYMKEYR VARAWMDARV TKIWAGSNEI MKELIGRDLG F
Validated NT Sequence
ttattagaag cccaggtcgc ggccgatgag ttccttcatg atctcgttgg agcctgccca gatcttggtg acgcgcgcgt ccatccaagc gcgtgcgacc cggtactcct tcatgtagcc gtacccgccg tgtagctgta cgcaggcgtc gatgacctcg ttctggacct gcgacgacca ccacttggcc ttggccgcgt caatcgcggt gagctcgccc ttgctgtggg ccagtactgc ctgatcgacg tacacctgtg ccgcttccag cttggtgacg agctcggcga tgaggaactt gttgtactgc aggctgccga tctgctgacc gaacgccttg cgctccttgg cgtattcgac ggtctcctcc agcacggcga ccgcgtgtgc gatgttggcg accgcacagc ccatccgctc ctgaggcagg cgctgcatca tcgagatgaa tccctggttg acctcgccga tgacgttctc ggcgggcacc cgcacatcgg tgaagaacaa ctcggcggta tcggcctcgg gctggcccac tttgtccagc ttgcggccgc gctcgaagcc gggcatgccc tcctcgaccg cgaacagggt gatgcccttg gccttcagct cgggcgcggt gcgtgccgcg acgacgacga gatcggctcg cgcaccgttg gtgatgaacg tcttggagcc gttgatgacc cagtcgtcgc cgtccttgac ggccgttgtc ttgagggccg ccagatcgga gccacccgag ggctcggtca tgccgatcgc ggtgatcatc tcgccgctac agaacttggg cagccagcgt tgcttctgct cgtcggtggt cagctcgacg aggtacgggg cgacgatgtc gaagtgaatg gacatcgagg aggccagggc cgcgctggag cgggccagtt cctcctgcag taccgcgttg tagcggtagt cgccggcgct gctgccgccg aactcctcgg ggacgtcgag gcccaggtaa ccctgcttac cggcctcttc ccagagctcg cggtcaatgt agcgctgctc gatgaatgtc tcggcgcgcg ggtcgacgtg gcgttgcaca aacgccttca cggactcgcg gaatgcctcg tggtcctcgg tgtagatggt ccgttgcatc gaaccaggac cctgggtctg agcttccagg gtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRTIYTED HEAFRESVKA FVQRHVDPRA ETFIEQRYID RELWEEAGKQ GYLGLDVPEE FGGSSAGDYR YNAVLQEELA RSSAALASSM SIHFDIVAPY LVELTTDEQK QRWLPKFCSG EMITAIGMTE PSGGSDLAAL KTTAVKDGDD WVINGSKTFI TNGARADLVV VAARTAPELK AKGITLFAVE EGMPGFERGR KLDKVGQPEA DTAELFFTDV RVPAENVIGE VNQGFISMMQ RLPQERMGCA VANIAHAVAV LEETVEYAKE RKAFGQQIGS LQYNKFLIAE LVTKLEAAQV YVDQAVLAHS KGELTAIDAA KAKWWSSQVQ NEVIDACVQL HGGYGYMKEY RVARAWMDAR VTKIWAGSNE IMKELIGRDL GF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAACGGACC ATCTACACCG AGGACCACGA GGCATTCCGC GAGTCCGTGA AGGCGTTTGT GCAACGCCAC GTCGACCCGC GCGCCGAGAC ATTCATCGAG CAGCGCTACA TTGACCGCGA GCTCTGGGAA GAGGCCGGTA AGCAGGGTTA CCTGGGCCTC GACGTCCCCG AGGAGTTCGG CGGCAGCAGC GCCGGCGACT ACCGCTACAA CGCGGTACTG CAGGAGGAAC TGGCCCGCTC CAGCGCGGCC CTGGCCTCCT CGATGTCCAT TCACTTCGAC ATCGTCGCCC CGTACCTCGT CGAGCTGACC ACCGACGAGC AGAAGCAACG CTGGCTGCCC AAGTTCTGTA GCGGCGAGAT GATCACCGCG ATCGGCATGA CCGAGCCCTC GGGTGGCTCC GATCTGGCGG CCCTCAAGAC AACGGCCGTC AAGGACGGCG ACGACTGGGT CATCAACGGC TCCAAGACGT TCATCACCAA CGGTGCGCGA GCCGATCTCG TCGTCGTCGC GGCACGCACC GCGCCCGAGC TGAAGGCCAA GGGCATCACC CTGTTCGCGG TCGAGGAGGG CATGCCCGGC TTCGAGCGCG GCCGCAAGCT GGACAAAGTG GGCCAGCCCG AGGCCGATAC CGCCGAGTTG TTCTTCACCG ATGTGCGGGT GCCCGCCGAG AACGTCATCG GCGAGGTCAA CCAGGGATTC ATCTCGATGA TGCAGCGCCT GCCTCAGGAG CGGATGGGCT GTGCGGTCGC CAACATCGCA CACGCGGTCG CCGTGCTGGA GGAGACCGTC GAATACGCCA AGGAGCGCAA GGCGTTCGGT CAGCAGATCG GCAGCCTGCA GTACAACAAG TTCCTCATCG CCGAGCTCGT CACCAAGCTG GAAGCGGCAC AGGTGTACGT CGATCAGGCA GTACTGGCCC ACAGCAAGGG CGAGCTCACC GCGATTGACG CGGCCAAGGC CAAGTGGTGG TCGTCGCAGG TCCAGAACGA GGTCATCGAC GCCTGCGTAC AGCTACACGG CGGGTACGGC TACATGAAGG AGTACCGGGT CGCACGCGCT TGGATGGACG CGCGCGTCAC CAAGATCTGG GCAGGCTCCA ACGAGATCAT GAAGGAACTC ATCGGCCGCG ACCTGGGCTT CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA