MysmA.00247.a

Long-chain specific acyl-CoA dehydrogenase

CENTER ID: MysmA.00247.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00247.a.A1.GE28586 Full length( MysmA.00247.a ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00247.a.A1.PS00525 Full length( MysmA.00247.a ) 1 380

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5070
RefSeq: YP_889443.1
UniProt: A0R2Q5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRLIYNEEH EAFRQTVKEY IERELVPHSE KWEAERIVDR SAYTAAGKYG LIGFNMPEEF GGGGSDDFRF NAIIDEEIAK SGVHGPALSL HNDVVGPYFK ELANDEQRRR WLPGITSGEL IIAIAMTEPG AGSDLAGIRT SAVRDGDDWI INGSKTFISS GINCDLCVVV ARTDPEAGHK GFSLFVVERG MEGFTRGRKL DKMGLHSQDT SELHFENVRV PHANLLGKEG RGFYHLMTNL PSERLSIAIS AIAGARAVFD ETLQYCKDRK AFGQPIGSFQ HNRFLLAEME TELEVTENYI DRCLQGVVDG ELTAVEAAKA KWWATETAKK IVDNCVQLHG GYGYMMEYRV ARAYVDGRIQ TIFGGTTEIM KEIIGRDLGV
NT Sequence
GTGAAGCGAC TCATCTACAA CGAAGAGCAC GAGGCGTTCC GCCAGACCGT CAAGGAGTAC ATCGAGCGTG AGCTCGTCCC GCACTCCGAG AAGTGGGAGG CCGAGCGCAT CGTCGACCGG TCCGCGTACA CGGCGGCCGG TAAGTACGGC CTCATCGGGT TCAACATGCC CGAGGAGTTC GGCGGCGGCG GGTCCGACGA CTTCCGGTTC AACGCGATCA TCGACGAGGA GATCGCCAAG TCCGGTGTGC ACGGTCCGGC GCTGAGCCTG CACAACGACG TCGTGGGCCC GTACTTCAAG GAACTGGCCA ACGACGAGCA GCGGCGGCGC TGGCTGCCCG GCATCACCAG CGGCGAGCTC ATCATCGCGA TCGCGATGAC CGAGCCCGGC GCGGGCAGCG ACCTGGCGGG CATCCGCACC TCGGCGGTGC GCGACGGTGA CGACTGGATC ATCAACGGCT CCAAGACGTT CATCTCGTCG GGCATCAACT GCGACCTGTG CGTCGTGGTG GCCCGCACCG ATCCCGAGGC CGGCCACAAG GGCTTCTCGC TGTTCGTGGT CGAGCGGGGC ATGGAGGGCT TCACCCGCGG TCGCAAGCTC GACAAGATGG GCCTGCACAG CCAGGACACC TCGGAGCTGC ACTTCGAGAA TGTCCGTGTG CCGCATGCCA ACCTGCTGGG CAAGGAAGGT CGCGGGTTCT ACCACCTGAT GACCAACCTG CCGTCGGAGC GCCTCTCGAT CGCCATCTCG GCCATCGCCG GTGCGCGCGC GGTGTTCGAC GAGACGCTGC AGTACTGCAA GGACCGCAAG GCCTTCGGCC AGCCGATCGG CAGCTTCCAG CACAACCGGT TCCTGCTCGC CGAGATGGAG ACCGAGCTCG AGGTCACCGA GAACTACATC GACCGCTGCC TGCAGGGTGT CGTCGACGGT GAGCTCACCG CGGTCGAGGC CGCCAAGGCC AAATGGTGGG CGACCGAGAC CGCCAAGAAG ATCGTCGACA ACTGCGTGCA GCTGCACGGC GGTTACGGCT ACATGATGGA GTACCGCGTG GCCCGCGCCT ACGTCGACGG ACGCATCCAG ACGATCTTCG GTGGCACCAC CGAGATCATG AAGGAGATCA TCGGCCGCGA CCTGGGTGTC
Details for MysmA.00247.a.A1.GE28586
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRLIYNE EHEAFRQTVK EYIERELVPH SEKWEAERIV DRSAYTAAGK YGLIGFNMPE EFGGGGSDDF RFNAIIDEEI AKSGVHGPAL SLHNDVVGPY FKELANDEQR RRWLPGITSG ELIIAIAMTE PGAGSDLAGI RTSAVRDGDD WIINGSKTFI SSGINCDLCV VVARTDPEAG HKGFSLFVVE RGMEGFTRGR KLDKMGLHSQ DTSELHFENV RVPHANLLGK EGRGFYHLMT NLPSERLSIA ISAIAGARAV FDETLQYCKD RKAFGQPIGS FQHNRFLLAE METELEVTEN YIDRCLQGVV DGELTAVEAA KAKWWATETA KKIVDNCVQL HGGYGYMMEY RVARAYVDGR IQTIFGGTTE IMKEIIGRDL GV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga agcgactcat ctacaacgaa gagcacgagg cgttccgcca gaccgtcaag gagtacatcg agcgtgagct cgtcccgcac tccgagaagt gggaggccga gcgcatcgtc gaccggtccg cgtacacggc ggccggtaag tacggcctca tcgggttcaa catgcccgag gagttcggcg gcggcgggtc cgacgacttc cggttcaacg cgatcatcga cgaggagatc gccaagtccg gtgtgcacgg tccggcgctg agcctgcaca acgacgtcgt gggcccgtac ttcaaggaac tggccaacga cgagcagcgg cggcgctggc tgcccggcat caccagcggc gagctcatca tcgcgatcgc gatgaccgag cccggcgcgg gcagcgacct ggcgggcatc cgcacctcgg cggtgcgcga cggtgacgac tggatcatca acggctccaa gacgttcatc tcgtcgggca tcaactgcga cctgtgcgtc gtggtggccc gcaccgatcc cgaggccggc cacaagggct tctcgctgtt cgtggtcgag cggggcatgg agggcttcac ccgcggtcgc aagctcgaca agatgggcct gcacagccag gacacctcgg agctgcactt cgagaatgtc cgtgtgccgc atgccaacct gctgggcaag gaaggtcgcg ggttctacca cctgatgacc aacctgccgt cggagcgcct ctcgatcgcc atctcggcca tcgccggtgc gcgcgcggtg ttcgacgaga cgctgcagta ctgcaaggac cgcaaggcct tcggccagcc gatcggcagc ttccagcaca accggttcct gctcgccgag atggagaccg agctcgaggt caccgagaac tacatcgacc gctgcctgca gggtgtcgtc gacggtgagc tcaccgcggt cgaggccgcc aaggccaaat ggtgggcgac cgagaccgcc aagaagatcg tcgacaactg cgtgcagctg cacggcggtt acggctacat gatggagtac cgcgtggccc gcgcctacgt cgacggacgc atccagacga tcttcggtgg caccaccgag atcatgaagg agatcatcgg ccgcgacctg ggtgtctaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRLIYNE EHEAFRQTVK EYIERELVPH SEKWEAERIV DRSAYTAAGK YGLIGFNMPE EFGGGGSDDF RFNAIIDEEI AKSGVHGPAL SLHNDVVGPY FKELANDEQR RRWLPGITSG ELIIAIAMTE PGAGSDLAGI RTSAVRDGDD WIINGSKTFI SSGINCDLCV VVARTDPEAG HKGFSLFVVE RGMEGFTRGR KLDKMGLHSQ DTSELHFENV RVPHANLLGK EGRGFYHLMT NLPSERLSIA ISAIAGARAV FDETLQYCKD RKAFGQPIGS FQHNRFLLAE METELEVTEN YIDRCLQGVV DGELTAVEAA KAKWWATETA KKIVDNCVQL HGGYGYMMEY RVARAYVDGR IQTIFGGTTE IMKEIIGRDL GV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagcga ctcatctaca acgaagagca cgaggcgttc cgccagaccg tcaaggagta catcgagcgt gagctcgtcc cgcactccga gaagtgggag gccgagcgca tcgtcgaccg gtccgcgtac acggcggccg gtaagtacgg cctcatcggg ttcaacatgc ccgaggagtt cggcggcggc gggtccgacg acttccggtt caacgcgatc atcgacgagg agatcgccaa gtccggtgtg cacggtccgg cgctgagcct gcacaacgac gtcgtgggcc cgtacttcaa ggaactggcc aacgacgagc agcggcggcg ctggctgccc ggcatcacca gcggcgagct catcatcgcg atcgcgatga ccgagcccgg cgcgggcagc gacctggcgg gcatccgcac ctcggcggtg cgcgacggtg acgactggat catcaacggc tccaagacgt tcatctcgtc gggcatcaac tgcgacctgt gcgtcgtggt ggcccgcacc gatcccgagg ccggccacaa gggcttctcg ctgttcgtgg tcgagcgggg catggagggc ttcacccgcg gtcgcaagct cgacaagatg ggcctgcaca gccaggacac ctcggagctg cacttcgaga atgtccgtgt gccgcatgcc aacctgctgg gcaaggaagg tcgcgggttc taccacctga tgaccaacct gccgtcggag cgcctctcga tcgccatctc ggccatcgcc ggtgcgcgcg cggtgttcga cgagacgctg cagtactgca aggaccgcaa ggccttcggc cagccgatcg gcagcttcca gcacaaccgg ttcctgctcg ccgagatgga gaccgagctc gaggtcaccg agaactacat cgaccgctgc ctgcagggtg tcgtcgacgg tgagctcacc gcggtcgagg ccgccaaggc caaatggtgg gcgaccgaga ccgccaagaa gatcgtcgac aactgcgtgc agctgcacgg cggttacggc tacatgatgg agtaccgcgt ggcccgcgcc tacgtcgacg gacgcatcca gacgatcttc ggtggcacca ccgagatcat gaaggagatc atcggccgcg acctgggtgt caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MysmA.00247.a.A1.PS00525
PURIFICATION DATe: 3/18/2010
CONCENTRATION: 42.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRLIYNE EHEAFRQTVK EYIERELVPH SEKWEAERIV DRSAYTAAGK YGLIGFNMPE EFGGGGSDDF RFNAIIDEEI AKSGVHGPAL SLHNDVVGPY FKELANDEQR RRWLPGITSG ELIIAIAMTE PGAGSDLAGI RTSAVRDGDD WIINGSKTFI SSGINCDLCV VVARTDPEAG HKGFSLFVVE RGMEGFTRGR KLDKMGLHSQ DTSELHFENV RVPHANLLGK EGRGFYHLMT NLPSERLSIA ISAIAGARAV FDETLQYCKD RKAFGQPIGS FQHNRFLLAE METELEVTEN YIDRCLQGVV DGELTAVEAA KAKWWATETA KKIVDNCVQL HGGYGYMMEY RVARAYVDGR IQTIFGGTTE IMKEIIGRDL GV
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga agcgactcat ctacaacgaa gagcacgagg cgttccgcca gaccgtcaag gagtacatcg agcgtgagct cgtcccgcac tccgagaagt gggaggccga gcgcatcgtc gaccggtccg cgtacacggc ggccggtaag tacggcctca tcgggttcaa catgcccgag gagttcggcg gcggcgggtc cgacgacttc cggttcaacg cgatcatcga cgaggagatc gccaagtccg gtgtgcacgg tccggcgctg agcctgcaca acgacgtcgt gggcccgtac ttcaaggaac tggccaacga cgagcagcgg cggcgctggc tgcccggcat caccagcggc gagctcatca tcgcgatcgc gatgaccgag cccggcgcgg gcagcgacct ggcgggcatc cgcacctcgg cggtgcgcga cggtgacgac tggatcatca acggctccaa gacgttcatc tcgtcgggca tcaactgcga cctgtgcgtc gtggtggccc gcaccgatcc cgaggccggc cacaagggct tctcgctgtt cgtggtcgag cggggcatgg agggcttcac ccgcggtcgc aagctcgaca agatgggcct gcacagccag gacacctcgg agctgcactt cgagaatgtc cgtgtgccgc atgccaacct gctgggcaag gaaggtcgcg ggttctacca cctgatgacc aacctgccgt cggagcgcct ctcgatcgcc atctcggcca tcgccggtgc gcgcgcggtg ttcgacgaga cgctgcagta ctgcaaggac cgcaaggcct tcggccagcc gatcggcagc ttccagcaca accggttcct gctcgccgag atggagaccg agctcgaggt caccgagaac tacatcgacc gctgcctgca gggtgtcgtc gacggtgagc tcaccgcggt cgaggccgcc aaggccaaat ggtgggcgac cgagaccgcc aagaagatcg tcgacaactg cgtgcagctg cacggcggtt acggctacat gatggagtac cgcgtggccc gcgcctacgt cgacggacgc atccagacga tcttcggtgg caccaccgag atcatgaagg agatcatcgg ccgcgacctg ggtgtctaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKRLIYNE EHEAFRQTVK EYIERELVPH SEKWEAERIV DRSAYTAAGK YGLIGFNMPE EFGGGGSDDF RFNAIIDEEI AKSGVHGPAL SLHNDVVGPY FKELANDEQR RRWLPGITSG ELIIAIAMTE PGAGSDLAGI RTSAVRDGDD WIINGSKTFI SSGINCDLCV VVARTDPEAG HKGFSLFVVE RGMEGFTRGR KLDKMGLHSQ DTSELHFENV RVPHANLLGK EGRGFYHLMT NLPSERLSIA ISAIAGARAV FDETLQYCKD RKAFGQPIGS FQHNRFLLAE METELEVTEN YIDRCLQGVV DGELTAVEAA KAKWWATETA KKIVDNCVQL HGGYGYMMEY RVARAYVDGR IQTIFGGTTE IMKEIIGRDL GV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAAGCGA CTCATCTACA ACGAAGAGCA CGAGGCGTTC CGCCAGACCG TCAAGGAGTA CATCGAGCGT GAGCTCGTCC CGCACTCCGA GAAGTGGGAG GCCGAGCGCA TCGTCGACCG GTCCGCGTAC ACGGCGGCCG GTAAGTACGG CCTCATCGGG TTCAACATGC CCGAGGAGTT CGGCGGCGGC GGGTCCGACG ACTTCCGGTT CAACGCGATC ATCGACGAGG AGATCGCCAA GTCCGGTGTG CACGGTCCGG CGCTGAGCCT GCACAACGAC GTCGTGGGCC CGTACTTCAA GGAACTGGCC AACGACGAGC AGCGGCGGCG CTGGCTGCCC GGCATCACCA GCGGCGAGCT CATCATCGCG ATCGCGATGA CCGAGCCCGG CGCGGGCAGC GACCTGGCGG GCATCCGCAC CTCGGCGGTG CGCGACGGTG ACGACTGGAT CATCAACGGC TCCAAGACGT TCATCTCGTC GGGCATCAAC TGCGACCTGT GCGTCGTGGT GGCCCGCACC GATCCCGAGG CCGGCCACAA GGGCTTCTCG CTGTTCGTGG TCGAGCGGGG CATGGAGGGC TTCACCCGCG GTCGCAAGCT CGACAAGATG GGCCTGCACA GCCAGGACAC CTCGGAGCTG CACTTCGAGA ATGTCCGTGT GCCGCATGCC AACCTGCTGG GCAAGGAAGG TCGCGGGTTC TACCACCTGA TGACCAACCT GCCGTCGGAG CGCCTCTCGA TCGCCATCTC GGCCATCGCC GGTGCGCGCG CGGTGTTCGA CGAGACGCTG CAGTACTGCA AGGACCGCAA GGCCTTCGGC CAGCCGATCG GCAGCTTCCA GCACAACCGG TTCCTGCTCG CCGAGATGGA GACCGAGCTC GAGGTCACCG AGAACTACAT CGACCGCTGC CTGCAGGGTG TCGTCGACGG TGAGCTCACC GCGGTCGAGG CCGCCAAGGC CAAATGGTGG GCGACCGAGA CCGCCAAGAA GATCGTCGAC AACTGCGTGC AGCTGCACGG CGGTTACGGC TACATGATGG AGTACCGCGT GGCCCGCGCC TACGTCGACG GACGCATCCA GACGATCTTC GGTGGCACCA CCGAGATCAT GAAGGAGATC ATCGGCCGCG ACCTGGGTGT CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA