SSGCID: Target Page

MypaA.00247.c

FadE20_1

CENTER ID:	MypaA.00247.c
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00247.c.A1.GE29280	Full length( MypaA.00247.c )		1	387

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.00247.c.A1.PS01004	Full length( MypaA.00247.c )		1	387

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.1818
RefSeq:	NP_960647.1
UniProt:	Q73Z90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSELFPDYRP RWETDQHREL RKHAAEFFRK EATPNQERWA AQHAVDREFW NKAGDAGLLG LDLPEEYGGA GGDFAMNVVV QEELAYAYDQ GFGFAVHSPI VAHYIWAYGS DEQRRRWLPK IISGEAVLAI AMTEPGTGSD LQSVKTTARR DGDFYVINGS KTFISNGTHC DLLVIVAKTD PSKGAAGVSL IVAETDENLA GFERGRVLAK MGQHAQDTRE LFFSDMRVPV ANLLGAQEGL GFYQLMEQLA RERLIIATIC EALAEVAVLE AIKYSREREA FGRPIGKFQH TKFVLAECKT EALAIKTMVD YAVGQYLDGK NDPATASMAK LFAAEKSDQI VDKCLQIFGG YGYMTEYPIA NMYTASRVNR IYGGTSEIMK EIISRSL

NT Sequence

ATGTCCGAAT TGTTTCCCGA TTACCGTCCG CGTTGGGAGA CCGACCAGCA CCGCGAGCTG CGCAAGCACG CCGCGGAGTT CTTCCGCAAG GAGGCCACCC CGAACCAGGA ACGCTGGGCC GCCCAACACG CGGTCGATCG CGAGTTCTGG AACAAAGCCG GGGATGCCGG TTTGCTGGGC CTGGACCTGC CCGAGGAATA CGGCGGCGCG GGCGGCGACT TCGCGATGAA CGTCGTCGTG CAGGAGGAGC TGGCCTACGC CTACGACCAG GGCTTTGGAT TCGCCGTGCA CTCGCCGATC GTCGCGCACT ACATCTGGGC CTATGGCAGC GACGAGCAGC GCAGGCGGTG GCTGCCCAAG ATCATCAGCG GTGAGGCGGT GTTGGCCATC GCGATGACCG AACCAGGTAC CGGGTCGGAT CTGCAATCGG TGAAGACCAC CGCGCGGCGC GACGGCGACT TCTACGTCAT CAACGGGTCG AAGACGTTCA TCTCCAATGG AACTCACTGC GACCTGTTGG TGATCGTGGC CAAGACCGAC CCGTCGAAGG GGGCTGCCGG GGTGTCGTTG ATCGTGGCCG AAACCGACGA GAACCTGGCG GGTTTCGAGC GTGGCCGGGT GCTGGCCAAG ATGGGCCAGC ACGCGCAGGA CACCCGCGAG CTGTTCTTCT CCGACATGCG GGTGCCGGTG GCCAACCTGC TCGGCGCGCA GGAGGGTCTG GGCTTTTACC AATTGATGGA GCAGCTTGCC CGCGAACGGC TCATCATCGC GACAATCTGC GAGGCGCTCG CCGAGGTCGC GGTGCTCGAG GCGATCAAGT ACTCGCGAGA GCGGGAGGCG TTCGGCCGGC CGATCGGCAA GTTCCAGCAC ACCAAATTCG TACTGGCCGA ATGCAAAACG GAGGCGCTGG CGATCAAGAC GATGGTGGAC TACGCCGTCG GGCAGTACCT CGACGGCAAG AACGACCCGG CCACCGCGTC GATGGCCAAG CTGTTCGCCG CCGAGAAGAG CGACCAGATC GTCGACAAGT GCCTGCAGAT CTTCGGGGGC TACGGCTACA TGACCGAGTA TCCGATCGCC AACATGTACA CCGCGTCGCG GGTCAACCGG ATCTATGGCG GTACCAGCGA AATCATGAAG GAAATCATCA GCCGCTCGCT G

Details for MypaA.00247.c.A1.GE29280

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ttattacagc gagcggctga tgatttcctt catgatttcg ctggtaccgc catagatccg gttgacccgc gacgcggtgt acatgttggc gatcggatac tcggtcatgt agccgtagcc cccgaagatc tgcaggcact tgtcgacgat ctggtcgctc ttctcggcgg cgaacagctt ggccatcgac gcggtggccg ggtcgttctt gccgtcgagg tactgcccga cggcgtagtc caccatcgtc ttgatcgcca gcgcctccgt tttgcattcg gccagtacga atttggtgtg ctggaacttg ccgatcggcc ggccgaacgc ctcccgctct cgcgagtact tgatcgcctc gagcaccgcg acctcggcga gcgcctcgca gattgtcgcg atgatgagcc gttcgcgggc aagctgctcc atcaattggt aaaagcccag accctcctgc gcgccgagca ggttggccac cggcacccgc atgtcggaga agaacagctc gcgggtgtcc tgcgcgtgct ggcccatctt ggccagcacc cggccacgct cgaaacccgc caggttctcg tcggtttcgg ccacgatcaa cgacaccccg gcagccccct tcgacgggtc ggtcttggcc acgatcacca acaggtcgca gtgagttcca ttggagatga acgtcttcga cccgttgatg acgtagaagt cgccgtcgcg ccgcgcggtg gtcttcaccg attgcagatc cgacccggta cctggttcgg tcatcgcgat ggccaacacc gcctcaccgc tgatgatctt gggcagccac cgcctgcgct gctcgtcgct gccataggcc cagatgtagt gcgcgacgat cggcgagtgc acggcgaatc caaagccctg gtcgtaggcg taggccagct cctcctgcac gacgacgttc atcgcgaagt cgccgcccgc gccgccgtat tcctcgggca ggtccaggcc cagcaaaccg gcatccccgg ctttgttcca gaactcgcga tcgaccgcgt gttgggcggc ccagcgttcc tggttcgggg tggcctcctt gcggaagaac tccgcggcgt gcttgcgcag ctcgcggtgc tggtcggtct cccaacgcgg acggtaatcg ggaaacaatt cggacatcga accaggaccc tgggtctgag cttccagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSELFPDYR PRWETDQHRE LRKHAAEFFR KEATPNQERW AAQHAVDREF WNKAGDAGLL GLDLPEEYGG AGGDFAMNVV VQEELAYAYD QGFGFAVHSP IVAHYIWAYG SDEQRRRWLP KIISGEAVLA IAMTEPGTGS DLQSVKTTAR RDGDFYVING SKTFISNGTH CDLLVIVAKT DPSKGAAGVS LIVAETDENL AGFERGRVLA KMGQHAQDTR ELFFSDMRVP VANLLGAQEG LGFYQLMEQL ARERLIIATI CEALAEVAVL EAIKYSRERE AFGRPIGKFQ HTKFVLAECK TEALAIKTMV DYAVGQYLDG KNDPATASMA KLFAAEKSDQ IVDKCLQIFG GYGYMTEYPI ANMYTASRVN RIYGGTSEIM KEIISRSL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgaattg tttcccgatt accgtccgcg ttgggagacc gaccagcacc gcgagctgcg caagcacgcc gcggagttct tccgcaagga ggccaccccg aaccaggaac gctgggccgc ccaacacgcg gtcgatcgcg agttctggaa caaagccggg gatgccggtt tgctgggcct ggacctgccc gaggaatacg gcggcgcggg cggcgacttc gcgatgaacg tcgtcgtgca ggaggagctg gcctacgcct acgaccaggg ctttggattc gccgtgcact cgccgatcgt cgcgcactac atctgggcct atggcagcga cgagcagcgc aggcggtggc tgcccaagat catcagcggt gaggcggtgt tggccatcgc gatgaccgaa ccaggtaccg ggtcggatct gcaatcggtg aagaccaccg cgcggcgcga cggcgacttc tacgtcatca acgggtcgaa gacgttcatc tccaatggaa ctcactgcga cctgttggtg atcgtggcca agaccgaccc gtcgaagggg gctgccgggg tgtcgttgat cgtggccgaa accgacgaga acctggcggg tttcgagcgt ggccgggtgc tggccaagat gggccagcac gcgcaggaca cccgcgagct gttcttctcc gacatgcggg tgccggtggc caacctgctc ggcgcgcagg agggtctggg cttttaccaa ttgatggagc agcttgcccg cgaacggctc atcatcgcga caatctgcga ggcgctcgcc gaggtcgcgg tgctcgaggc gatcaagtac tcgcgagagc gggaggcgtt cggccggccg atcggcaagt tccagcacac caaattcgta ctggccgaat gcaaaacgga ggcgctggcg atcaagacga tggtggacta cgccgtcggg cagtacctcg acggcaagaa cgacccggcc accgcgtcga tggccaagct gttcgccgcc gagaagagcg accagatcgt cgacaagtgc ctgcagatct tcgggggcta cggctacatg accgagtatc cgatcgccaa catgtacacc gcgtcgcggg tcaaccggat ctatggcggt accagcgaaa tcatgaagga aatcatcagc cgctcgctga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MypaA.00247.c.A1.PS01004

PURIFICATION DATe:	3/14/2011
CONCENTRATION:	36.35mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCCGAATTG TTTCCCGATT ACCGTCCGCG TTGGGAGACC GACCAGCACC GCGAGCTGCG CAAGCACGCC GCGGAGTTCT TCCGCAAGGA GGCCACCCCG AACCAGGAAC GCTGGGCCGC CCAACACGCG GTCGATCGCG AGTTCTGGAA CAAAGCCGGG GATGCCGGTT TGCTGGGCCT GGACCTGCCC GAGGAATACG GCGGCGCGGG CGGCGACTTC GCGATGAACG TCGTCGTGCA GGAGGAGCTG GCCTACGCCT ACGACCAGGG CTTTGGATTC GCCGTGCACT CGCCGATCGT CGCGCACTAC ATCTGGGCCT ATGGCAGCGA CGAGCAGCGC AGGCGGTGGC TGCCCAAGAT CATCAGCGGT GAGGCGGTGT TGGCCATCGC GATGACCGAA CCAGGTACCG GGTCGGATCT GCAATCGGTG AAGACCACCG CGCGGCGCGA CGGCGACTTC TACGTCATCA ACGGGTCGAA GACGTTCATC TCCAATGGAA CTCACTGCGA CCTGTTGGTG ATCGTGGCCA AGACCGACCC GTCGAAGGGG GCTGCCGGGG TGTCGTTGAT CGTGGCCGAA ACCGACGAGA ACCTGGCGGG TTTCGAGCGT GGCCGGGTGC TGGCCAAGAT GGGCCAGCAC GCGCAGGACA CCCGCGAGCT GTTCTTCTCC GACATGCGGG TGCCGGTGGC CAACCTGCTC GGCGCGCAGG AGGGTCTGGG CTTTTACCAA TTGATGGAGC AGCTTGCCCG CGAACGGCTC ATCATCGCGA CAATCTGCGA GGCGCTCGCC GAGGTCGCGG TGCTCGAGGC GATCAAGTAC TCGCGAGAGC GGGAGGCGTT CGGCCGGCCG ATCGGCAAGT TCCAGCACAC CAAATTCGTA CTGGCCGAAT GCAAAACGGA GGCGCTGGCG ATCAAGACGA TGGTGGACTA CGCCGTCGGG CAGTACCTCG ACGGCAAGAA CGACCCGGCC ACCGCGTCGA TGGCCAAGCT GTTCGCCGCC GAGAAGAGCG ACCAGATCGT CGACAAGTGC CTGCAGATCT TCGGGGGCTA CGGCTACATG ACCGAGTATC CGATCGCCAA CATGTACACC GCGTCGCGGG TCAACCGGAT CTATGGCGGT ACCAGCGAAA TCATGAAGGA AATCATCAGC CGCTCGCTGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A