MypaA.01326.b
Putative carveol dehydrogenase
| CENTER ID: | MypaA.01326.b |
| ORGANISM: | Mycobacterium paratuberculosis ATCC BAA-968 / K-10 |
| ASSOCIATED DISEASE: | Johne's disease |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MypaA.01326.b.A1.GE29573 | Full length( MypaA.01326.b ) | 1 | 275 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3PGX
DEPOSITED: 11/2/2010
DETERMINATION: XRay
CLONE: MypaA.01326.b.A1.GE29573
PROTEIN: MypaA.01326.b.A1.PW29471
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|262316.17.peg.4418 |
| RefSeq: | NP_963080.1 |
| UniProt: | Q73SC8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGQAGSLQG RVAFITGAAR GQGRSHAVRL AAEGADIIAC DICAPVSASV TYAPASPEDL DETARLVEDQ GRKALTRVLD VRDDAALREL VADGMEQFGR LDVVVANAGV LSWGRVWELT DEQWDTVIGV NLTGTWRTLR ATVPAMIEAG NGGSIVVVSS SAGLKATPGN GHYSASKHGL TALTNTLAIE LGEYGIRVNS IHPYSVETPM IEPEAMMEIF ARHPSFVHSF PPMPVQPNGF MTADEVADVV AWLAGDGSGT LTGTQIPVDK GALKY
NT Sequence
GTGGCGGGAC AGGCCGGATC ACTGCAGGGA CGGGTGGCAT TCATCACCGG GGCGGCCCGC GGGCAGGGGC GCTCGCATGC GGTGCGGCTG GCCGCCGAGG GCGCCGACAT CATCGCGTGC GACATCTGCG CGCCGGTGTC GGCCAGCGTC ACCTACGCCC CGGCCAGCCC CGAGGACCTC GACGAGACCG CCCGGCTGGT GGAAGATCAG GGCCGCAAGG CCTTGACCCG CGTCCTCGAC GTCCGCGACG ACGCCGCCCT GCGGGAGCTG GTCGCCGACG GGATGGAGCA GTTCGGCCGG CTGGACGTGG TGGTGGCCAA CGCCGGCGTG CTGAGCTGGG GCCGCGTCTG GGAACTCACC GACGAGCAGT GGGACACCGT CATCGGCGTC AACCTGACCG GCACCTGGCG CACCCTGCGC GCCACCGTGC CGGCGATGAT CGAGGCGGGC AACGGCGGCT CCATCGTGGT GGTCAGCTCC TCGGCCGGGC TCAAGGCCAC CCCGGGCAAC GGCCACTACT CGGCCAGCAA ACACGGGTTG ACCGCGCTCA CCAACACGCT GGCCATCGAG CTCGGCGAAT ACGGTATCCG GGTCAACTCC ATCCACCCGT ACTCGGTCGA AACCCCGATG ATCGAGCCCG AGGCGATGAT GGAGATCTTC GCCCGCCATC CCAGCTTCGT GCACAGCTTC CCGCCAATGC CGGTGCAGCC CAACGGTTTC ATGACCGCCG ACGAGGTCGC CGACGTGGTG GCCTGGCTGG CCGGCGACGG CTCGGGCACG CTGACCGGCA CGCAGATCCC CGTTGATAAG GGCGCCCTAA AGTAC
Details for MypaA.01326.b.A1.GE29573
| HARVESTED ON: | 6/15/2010 |
| SEQUENCED ON: | 6/27/2010 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 34kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGQAGSL QGRVAFITGA ARGQGRSHAV RLAAEGADII ACDICAPVSA SVTYAPASPE DLDETARLVE DQGRKALTRV LDVRDDAALR ELVADGMEQF GRLDVVVANA GVLSWGRVWE LTDEQWDTVI GVNLTGTWRT LRATVPAMIE AGNGGSIVVV SSSAGLKATP GNGHYSASKH GLTALTNTLA IELGEYGIRV NSIHPYSVET PMIEPEAMME IFARHPSFVH SFPPMPVQPN GFMTADEVAD VVAWLAGDGS GTLTGTQIPV DKGALKY
Validated NT Sequence
ttagggcgcc cttatcaacg gggatctgcg tgccggtcag cgtgcccgag ccgtcgccgg ccagccaggc caccacgtcg gcgacctcgt cggcggtcat gaaaccgttg ggctgcaccg gcattggcgg gaagctgtgc acgaagctgg gatggcgggc gaagatctcc atcatcgcct cgggctcgat catcggggtt tcgaccgagt acgggtggat ggagttgacc cggataccgt attcgccgag ctcgatggcc agcgtgttgg tgagcgcggt caacccgtgt ttgctggccg agtagtggcc gttgcccggg gtggccttga gcccggccga ggagctgacc accacgatgg agccgccgtt gcccgcctcg atcatcgccg gcacggtggc gcgcagggtg cgccaggtgc cggtcaggtt gacgccgatg acggtgtccc actgctcgtc ggtgagttcc cagacgcggc cccagctcag cacgccggcg ttggccacca ccacgtccag ccggccgaac tgctccatcc cgtcggcgac cagctcccgc agggcggcgt cgtcgcggac gtcgaggacg cgggtcaagg ccttgcggcc ctgatcttcc accagccggg cggtctcgtc gaggtcctcg gggctggccg gggcgtaggt gacgctggcc gacaccggcg cgcagatgtc gcacgcgatg atgtcggcgc cctcggcggc cagccgcacc gcatgcgagc gcccctgccc gcgggccgcc ccggtgatga atgccacccg tccctgcagt gatccggcct gtcccgccac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGQAGSL QGRVAFITGA ARGQGRSHAV RLAAEGADII ACDICAPVSA SVTYAPASPE DLDETARLVE DQGRKALTRV LDVRDDAALR ELVADGMEQF GRLDVVVANA GVLSWGRVWE LTDEQWDTVI GVNLTGTWRT LRATVPAMIE AGNGGSIVVV SSSAGLKATP GNGHYSASKH GLTALTNTLA IELGEYGIRV NSIHPYSVET PMIEPEAMME IFARHPSFVH SFPPMPVQPN GFMTADEVAD VVAWLAGDGS GTLTGTQIPV DKGALKY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcggga caggccggat cactgcaggg acgggtggca ttcatcaccg gggcggcccg cgggcagggg cgctcgcatg cggtgcggct ggccgccgag ggcgccgaca tcatcgcgtg cgacatctgc gcgccggtgt cggccagcgt cacctacgcc ccggccagcc ccgaggacct cgacgagacc gcccggctgg tggaagatca gggccgcaag gccttgaccc gcgtcctcga cgtccgcgac gacgccgccc tgcgggagct ggtcgccgac gggatggagc agttcggccg gctggacgtg gtggtggcca acgccggcgt gctgagctgg ggccgcgtct gggaactcac cgacgagcag tgggacaccg tcatcggcgt caacctgacc ggcacctggc gcaccctgcg cgccaccgtg ccggcgatga tcgaggcggg caacggcggc tccatcgtgg tggtcagctc ctcggccggg ctcaaggcca ccccgggcaa cggccactac tcggccagca aacacgggtt gaccgcgctc accaacacgc tggccatcga gctcggcgaa tacggtatcc gggtcaactc catccacccg tactcggtcg aaaccccgat gatcgagccc gaggcgatga tggagatctt cgcccgccat cccagcttcg tgcacagctt cccgccaatg ccggtgcagc ccaacggttt catgaccgcc gacgaggtcg ccgacgtggt ggcctggctg gccggcgacg gctcgggcac gctgaccggc acgcagatcc ccgttgataa gggcgcccta aagtacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa