MyabA.01326.a

Putative short chain dehydrogenase/reductase

CENTER ID: MyabA.01326.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01326.a.A1.GE34906 Full length( MyabA.01326.a ) 1 276
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01326.a.A1.PW35521 Full length( MyabA.01326.a ) 1 276

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.132
RefSeq: YP_001700854.1
UniProt: B1MED6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSQHDKPLA GRVAYITGAG RGQGRAHAIR LAADGADIVA IDTCDKPSPY NDYPATTSHE FDETISAVRD LGRDIITERV DVRDLAGHQK VIQEAITRFG RLDIVVANAG IASWARLWEI EADQWRDVID VNLTGVWNTI KAAVPAMIEA GNGGSIITVS SSAGIKAMPG CGHYVAAKFG VVGLTNSLAI ELGEYGIRVN SIHPYGTNTP LAGDQSVLRM FEMYPGYLQS FSQTLVDNDR LIEPAEIAEV VAWLAGDASG VMTGAQIPVD KGYLKR
NT Sequence
ATGACCAGTC AGCATGACAA GCCCCTTGCC GGGCGCGTCG CGTACATCAC CGGGGCCGGG CGCGGCCAGG GCCGGGCGCA CGCCATCCGG TTGGCGGCCG ACGGCGCCGA CATCGTGGCG ATCGACACCT GTGACAAGCC CTCCCCGTAC AACGACTACC CGGCGACTAC GTCACATGAG TTCGATGAGA CCATTTCCGC GGTAAGGGAT CTGGGCCGCG ACATCATCAC CGAACGCGTC GATGTACGCG ATCTTGCGGG GCATCAGAAG GTGATCCAGG AAGCGATCAC CCGGTTCGGA CGCCTGGACA TCGTGGTGGC CAACGCGGGT ATCGCCAGCT GGGCGCGGCT CTGGGAGATC GAGGCCGACC AATGGCGCGA TGTCATCGAC GTCAATCTGA CCGGAGTGTG GAACACCATC AAAGCCGCGG TCCCGGCGAT GATCGAGGCA GGCAACGGTG GCTCCATCAT CACGGTGAGT TCCTCGGCGG GTATCAAGGC CATGCCGGGG TGCGGGCACT ATGTAGCGGC CAAGTTCGGT GTGGTGGGTT TGACCAACTC ACTGGCGATC GAATTGGGCG AGTATGGGAT TCGAGTCAAT TCGATTCATC CTTATGGGAC CAACACACCC TTGGCGGGCG ATCAGTCGGT ATTGAGGATG TTCGAGATGT ATCCCGGATA TCTGCAGAGC TTTAGCCAGA CACTGGTCGA CAACGACAGG CTGATCGAGC CCGCCGAGAT TGCCGAGGTG GTGGCGTGGC TGGCCGGCGA TGCGTCCGGG GTCATGACCG GCGCACAGAT TCCCGTCGAC AAGGGATACC TCAAGCGA
Details for MyabA.01326.a.A1.GE34906
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTSQHDKPL AGRVAYITGA GRGQGRAHAI RLAADGADIV AIDTCDKPSP YNDYPATTSH EFDETISAVR DLGRDIITER VDVRDLAGHQ KVIQEAITRF GRLDIVVANA GIASWARLWE IEADQWRDVI DVNLTGVWNT IKAAVPAMIE AGNGGSIITV SSSAGIKAMP GCGHYVAAKF GVVGLTNSLA IELGEYGIRV NSIHPYGTNT PLAGDQSVLR MFEMYPGYLQ SFSQTLVDND RLIEPAEIAE VVAWLAGDAS GVMTGAQIPV DKGYL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca gtcagcatga caagcccctt gccgggcgcg tcgcgtacat caccggggcc gggcgcggcc agggccgggc gcacgccatc cggttggcgg ccgacggcgc cgacatcgtg gcgatcgaca cctgtgacaa gccctccccg tacaacgact acccggcgac tacgtcacat gagttcgatg agaccatttc cgcggtaagg gatctgggcc gcgacatcat caccgaacgc gtcgatgtac gcgatcttgc ggggcatcag aaggtgatcc aggaagcgat cacccggttc ggacgcctgg acatcgtggt ggccaacgcg ggtatcgcca gctgggcgcg gctctgggag atcgaggccg accaatggcg cgatgtcatc gacgtcaatc tgaccggagt gtggaacacc atcaaagccg cggtcccggc gatgatcgag gcaggcaacg gtggctccat catcacggtg agttcctcgg cgggtatcaa ggccatgccg gggtgcgggc actatgtagc ggccaagttc ggtgtggtgg gtttgaccaa ctcactggcg atcgaattgg gcgagtatgg gattcgagtc aattcgattc atccttatgg gaccaacaca cccttggcgg gcgatcagtc ggtattgagg atgttcgaga tgtatcccgg atatctgcag agctttagcc agacactggt cgacaacgac aggctgatcg agcccgccga gattgccgag gtggtggcgt ggctggccgg cgatgcgtcc ggggtcatga ccggcgcaca gattcccgtc gacaagggat acctca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSQHDKPL AGRVAYITGA GRGQGRAHAI RLAADGADIV AIDTCDKPSP YNDYPATTSH EFDETISAVR DLGRDIITER VDVRDLAGHQ KVIQEAITRF GRLDIVVANA GIASWARLWE IEADQWRDVI DVNLTGVWNT IKAAVPAMIE AGNGGSIITV SSSAGIKAMP GCGHYVAAKF GVVGLTNSLA IELGEYGIRV NSIHPYGTNT PLAGDQSVLR MFEMYPGYLQ SFSQTLVDND RLIEPAEIAE VVAWLAGDAS GVMTGAQIPV DKGYLKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccagtcag catgacaagc cccttgccgg gcgcgtcgcg tacatcaccg gggccgggcg cggccagggc cgggcgcacg ccatccggtt ggcggccgac ggcgccgaca tcgtggcgat cgacacctgt gacaagccct ccccgtacaa cgactacccg gcgactacgt cacatgagtt cgatgagacc atttccgcgg taagggatct gggccgcgac atcatcaccg aacgcgtcga tgtacgcgat cttgcggggc atcagaaggt gatccaggaa gcgatcaccc ggttcggacg cctggacatc gtggtggcca acgcgggtat cgccagctgg gcgcggctct gggagatcga ggccgaccaa tggcgcgatg tcatcgacgt caatctgacc ggagtgtgga acaccatcaa agccgcggtc ccggcgatga tcgaggcagg caacggtggc tccatcatca cggtgagttc ctcggcgggt atcaaggcca tgccggggtg cgggcactat gtagcggcca agttcggtgt ggtgggtttg accaactcac tggcgatcga attgggcgag tatgggattc gagtcaattc gattcatcct tatgggacca acacaccctt ggcgggcgat cagtcggtat tgaggatgtt cgagatgtat cccggatatc tgcagagctt tagccagaca ctggtcgaca acgacaggct gatcgagccc gccgagattg ccgaggtggt ggcgtggctg gccggcgatg cgtccggggt catgaccggc gcacagattc ccgtcgacaa gggatacctc aagcgataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MyabA.01326.a.A1.PW35521
PURIFICATION DATe: 9/4/2012
CONCENTRATION: 4.86mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTSQHDKPL AGRVAYITGA GRGQGRAHAI RLAADGADIV AIDTCDKPSP YNDYPATTSH EFDETISAVR DLGRDIITER VDVRDLAGHQ KVIQEAITRF GRLDIVVANA GIASWARLWE IEADQWRDVI DVNLTGVWNT IKAAVPAMIE AGNGGSIITV SSSAGIKAMP GCGHYVAAKF GVVGLTNSLA IELGEYGIRV NSIHPYGTNT PLAGDQSVLR MFEMYPGYLQ SFSQTLVDND RLIEPAEIAE VVAWLAGDAS GVMTGAQIPV DKGYL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca gtcagcatga caagcccctt gccgggcgcg tcgcgtacat caccggggcc gggcgcggcc agggccgggc gcacgccatc cggttggcgg ccgacggcgc cgacatcgtg gcgatcgaca cctgtgacaa gccctccccg tacaacgact acccggcgac tacgtcacat gagttcgatg agaccatttc cgcggtaagg gatctgggcc gcgacatcat caccgaacgc gtcgatgtac gcgatcttgc ggggcatcag aaggtgatcc aggaagcgat cacccggttc ggacgcctgg acatcgtggt ggccaacgcg ggtatcgcca gctgggcgcg gctctgggag atcgaggccg accaatggcg cgatgtcatc gacgtcaatc tgaccggagt gtggaacacc atcaaagccg cggtcccggc gatgatcgag gcaggcaacg gtggctccat catcacggtg agttcctcgg cgggtatcaa ggccatgccg gggtgcgggc actatgtagc ggccaagttc ggtgtggtgg gtttgaccaa ctcactggcg atcgaattgg gcgagtatgg gattcgagtc aattcgattc atccttatgg gaccaacaca cccttggcgg gcgatcagtc ggtattgagg atgttcgaga tgtatcccgg atatctgcag agctttagcc agacactggt cgacaacgac aggctgatcg agcccgccga gattgccgag gtggtggcgt ggctggccgg cgatgcgtcc ggggtcatga ccggcgcaca gattcccgtc gacaagggat acctca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSQHDKPL AGRVAYITGA GRGQGRAHAI RLAADGADIV AIDTCDKPSP YNDYPATTSH EFDETISAVR DLGRDIITER VDVRDLAGHQ KVIQEAITRF GRLDIVVANA GIASWARLWE IEADQWRDVI DVNLTGVWNT IKAAVPAMIE AGNGGSIITV SSSAGIKAMP GCGHYVAAKF GVVGLTNSLA IELGEYGIRV NSIHPYGTNT PLAGDQSVLR MFEMYPGYLQ SFSQTLVDND RLIEPAEIAE VVAWLAGDAS GVMTGAQIPV DKGYLKR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCAGTCAG CATGACAAGC CCCTTGCCGG GCGCGTCGCG TACATCACCG GGGCCGGGCG CGGCCAGGGC CGGGCGCACG CCATCCGGTT GGCGGCCGAC GGCGCCGACA TCGTGGCGAT CGACACCTGT GACAAGCCCT CCCCGTACAA CGACTACCCG GCGACTACGT CACATGAGTT CGATGAGACC ATTTCCGCGG TAAGGGATCT GGGCCGCGAC ATCATCACCG AACGCGTCGA TGTACGCGAT CTTGCGGGGC ATCAGAAGGT GATCCAGGAA GCGATCACCC GGTTCGGACG CCTGGACATC GTGGTGGCCA ACGCGGGTAT CGCCAGCTGG GCGCGGCTCT GGGAGATCGA GGCCGACCAA TGGCGCGATG TCATCGACGT CAATCTGACC GGAGTGTGGA ACACCATCAA AGCCGCGGTC CCGGCGATGA TCGAGGCAGG CAACGGTGGC TCCATCATCA CGGTGAGTTC CTCGGCGGGT ATCAAGGCCA TGCCGGGGTG CGGGCACTAT GTAGCGGCCA AGTTCGGTGT GGTGGGTTTG ACCAACTCAC TGGCGATCGA ATTGGGCGAG TATGGGATTC GAGTCAATTC GATTCATCCT TATGGGACCA ACACACCCTT GGCGGGCGAT CAGTCGGTAT TGAGGATGTT CGAGATGTAT CCCGGATATC TGCAGAGCTT TAGCCAGACA CTGGTCGACA ACGACAGGCT GATCGAGCCC GCCGAGATTG CCGAGGTGGT GGCGTGGCTG GCCGGCGATG CGTCCGGGGT CATGACCGGC GCACAGATTC CCGTCGACAA GGGATACCTC AAGCGATAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA