MymaA.01326.a

Carveol-like dehydrogenase

CENTER ID: MymaA.01326.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01326.a.A1.GE30166 Full length( MymaA.01326.a ) 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.01326.a.A1.PW30039 Full length( MymaA.01326.a ) 1 270

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.988
RefSeq: YP_001849236.1
UniProt: B2HRR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGALDGRVAL ITGGARGQGR AHALALAGQG ADIALADAPG PMAELTYPLG SEEDLLATAE LVGQLGRRCL PMVVDVRDAA QVNTAVERTV RELGSLDIVL ANAGIVSTGR LEEVSDQVWQ QLMDTNLTGV FHTLRAAIPV MRQQRFGRIV ATSSMGGRMG IPELAAYNAT KWGIIGLIKS VALEVAKEGI TANVICPTTT QTPMVQPAGI GDDQEVPDDL VRRMMKANPI PQPWLQPEDV SRGVVYLVTD PGVITGSVLE VGLGGSARMH
NT Sequence
ATGGGTGCCT TGGACGGACG TGTCGCCTTG ATCACCGGCG GAGCCCGCGG GCAGGGCCGC GCGCACGCGC TGGCCCTGGC GGGCCAGGGG GCCGATATCG CCCTCGCCGA CGCGCCGGGC CCGATGGCGG AGCTGACCTA CCCGTTGGGC AGCGAGGAAG ACCTGCTCGC CACCGCCGAA CTCGTCGGCC AGCTGGGCCG GCGTTGCCTG CCGATGGTGG TCGACGTGCG CGACGCGGCC CAGGTGAACA CCGCGGTTGA GCGGACGGTG CGCGAGCTGG GCAGCCTCGA TATCGTGCTG GCCAATGCCG GCATCGTCAG CACCGGGCGC CTCGAAGAGG TAAGTGACCA GGTTTGGCAG CAGCTGATGG ACACCAACCT GACCGGCGTG TTCCACACCC TGCGCGCGGC CATTCCGGTG ATGCGCCAAC AACGCTTCGG CAGGATCGTG GCGACATCGT CGATGGGCGG CCGAATGGGC ATCCCGGAAT TGGCGGCCTA CAACGCCACC AAGTGGGGGA TCATCGGCCT GATCAAGTCC GTCGCCCTCG AGGTCGCCAA GGAAGGCATC ACGGCCAACG TCATCTGTCC GACCACGACG CAGACACCGA TGGTGCAGCC CGCCGGGATC GGAGATGATC AGGAAGTTCC CGACGATCTG GTGCGGCGCA TGATGAAGGC CAACCCCATC CCTCAACCGT GGCTGCAACC GGAGGACGTC AGCCGCGGGG TGGTCTATCT GGTCACCGAC CCGGGTGTCA TCACCGGCAG CGTGCTGGAA GTCGGGCTCG GCGGCAGCGC TCGCATGCAC
Details for MymaA.01326.a.A1.GE30166
HARVESTED ON: 8/15/2010
SEQUENCED ON: 8/25/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGALDGRVA LITGGARGQG RAHALALAGQ GADIALADAP GPMAELTYPL GSEEDLLATA ELVGQLGRRC LPMVVDVRDA AQVNTAVERT VRELGSLDIV LANAGIVSTG RLEEVSDQVW QQLMDTNLTG VFHTLRAAIP VMRQQRFGRI VATSSMGGRM GIPELAAYNA TKWGIIGLIK SVALEVAKEG ITANVICPTT TQTPMVQPAG IGDDQEVPDD LVRRMMKANP IPQPWLQPED VSRGVVYLVT DPGVITGSVL EVGLGGSARM H
Validated NT Sequence
ttattagtgc atgcgagcgc tgccgccgag cccgacttcc agcacgctgc cggtgatgac acccgggtcg gtgaccagat agaccacccc gcggctgacg tcctccggtt gcagccacgg ttgagggatg gggttggcct tcatcatgcg ccgcaccaga tcgtcgggaa cttcctgatc atctccgatc ccggcgggct gcaccatcgg tgtctgcgtc gtggtcggac agatgacgtt ggccgtgatg ccttccttgg cgacctcgag ggcgacggac ttgatcaggc cgatgatccc ccacttggtg gcgttgtagg ccgccaattc cgggatgccc attcggccgc ccatcgacga tgtcgccacg atcctgccga agcgttgttg gcgcatcacc ggaatggccg cgcgcagggt gtggaacacg ccggtcaggt tggtgtccat cagctgctgc caaacctggt cacttacctc ttcgaggcgc ccggtgctga cgatgccggc attggccagc acgatatcga ggctgcccag ctcgcgcacc gtccgctcaa ccgcggtgtt cacctgggcc gcgtcgcgca cgtcgaccac catcggcagg caacgccggc ccagctggcc gacgagttcg gcggtggcga gcaggtcttc ctcgctgccc aacgggtagg tcagctccgc catcgggccc ggcgcgtcgg cgagggcgat atcggccccc tggcccgcca gggccagcgc gtgcgcgcgg ccctgcccgc gggctccgcc ggtgatcaag gcgacacgtc cgtccaaggc acccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGALDGRVA LITGGARGQG RAHALALAGQ GADIALADAP GPMAELTYPL GSEEDLLATA ELVGQLGRRC LPMVVDVRDA AQVNTAVERT VRELGSLDIV LANAGIVSTG RLEEVSDQVW QQLMDTNLTG VFHTLRAAIP VMRQQRFGRI VATSSMGGRM GIPELAAYNA TKWGIIGLIK SVALEVAKEG ITANVICPTT TQTPMVQPAG IGDDQEVPDD LVRRMMKANP IPQPWLQPED VSRGVVYLVT DPGVITGSVL EVGLGGSARM H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgccttg gacggacgtg tcgccttgat caccggcgga gcccgcgggc agggccgcgc gcacgcgctg gccctggcgg gccagggggc cgatatcgcc ctcgccgacg cgccgggccc gatggcggag ctgacctacc cgttgggcag cgaggaagac ctgctcgcca ccgccgaact cgtcggccag ctgggccggc gttgcctgcc gatggtggtc gacgtgcgcg acgcggccca ggtgaacacc gcggttgagc ggacggtgcg cgagctgggc agcctcgata tcgtgctggc caatgccggc atcgtcagca ccgggcgcct cgaagaggta agtgaccagg tttggcagca gctgatggac accaacctga ccggcgtgtt ccacaccctg cgcgcggcca ttccggtgat gcgccaacaa cgcttcggca ggatcgtggc gacatcgtcg atgggcggcc gaatgggcat cccggaattg gcggcctaca acgccaccaa gtgggggatc atcggcctga tcaagtccgt cgccctcgag gtcgccaagg aaggcatcac ggccaacgtc atctgtccga ccacgacgca gacaccgatg gtgcagcccg ccgggatcgg agatgatcag gaagttcccg acgatctggt gcggcgcatg atgaaggcca accccatccc tcaaccgtgg ctgcaaccgg aggacgtcag ccgcggggtg gtctatctgg tcaccgaccc gggtgtcatc accggcagcg tgctggaagt cgggctcggc ggcagcgctc gcatgcacaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MymaA.01326.a.A1.PW30039
PURIFICATION DATe: 11/15/2010
CONCENTRATION: 29.35mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGALDGRVA LITGGARGQG RAHALALAGQ GADIALADAP GPMAELTYPL GSEEDLLATA ELVGQLGRRC LPMVVDVRDA AQVNTAVERT VRELGSLDIV LANAGIVSTG RLEEVSDQVW QQLMDTNLTG VFHTLRAAIP VMRQQRFGRI VATSSMGGRM GIPELAAYNA TKWGIIGLIK SVALEVAKEG ITANVICPTT TQTPMVQPAG IGDDQEVPDD LVRRMMKANP IPQPWLQPED VSRGVVYLVT DPGVITGSVL EVGLGGSARM H
Validated NT Sequence
ttattagtgc atgcgagcgc tgccgccgag cccgacttcc agcacgctgc cggtgatgac acccgggtcg gtgaccagat agaccacccc gcggctgacg tcctccggtt gcagccacgg ttgagggatg gggttggcct tcatcatgcg ccgcaccaga tcgtcgggaa cttcctgatc atctccgatc ccggcgggct gcaccatcgg tgtctgcgtc gtggtcggac agatgacgtt ggccgtgatg ccttccttgg cgacctcgag ggcgacggac ttgatcaggc cgatgatccc ccacttggtg gcgttgtagg ccgccaattc cgggatgccc attcggccgc ccatcgacga tgtcgccacg atcctgccga agcgttgttg gcgcatcacc ggaatggccg cgcgcagggt gtggaacacg ccggtcaggt tggtgtccat cagctgctgc caaacctggt cacttacctc ttcgaggcgc ccggtgctga cgatgccggc attggccagc acgatatcga ggctgcccag ctcgcgcacc gtccgctcaa ccgcggtgtt cacctgggcc gcgtcgcgca cgtcgaccac catcggcagg caacgccggc ccagctggcc gacgagttcg gcggtggcga gcaggtcttc ctcgctgccc aacgggtagg tcagctccgc catcgggccc ggcgcgtcgg cgagggcgat atcggccccc tggcccgcca gggccagcgc gtgcgcgcgg ccctgcccgc gggctccgcc ggtgatcaag gcgacacgtc cgtccaaggc acccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGALDGRVA LITGGARGQG RAHALALAGQ GADIALADAP GPMAELTYPL GSEEDLLATA ELVGQLGRRC LPMVVDVRDA AQVNTAVERT VRELGSLDIV LANAGIVSTG RLEEVSDQVW QQLMDTNLTG VFHTLRAAIP VMRQQRFGRI VATSSMGGRM GIPELAAYNA TKWGIIGLIK SVALEVAKEG ITANVICPTT TQTPMVQPAG IGDDQEVPDD LVRRMMKANP IPQPWLQPED VSRGVVYLVT DPGVITGSVL EVGLGGSARM H
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTGCCTTG GACGGACGTG TCGCCTTGAT CACCGGCGGA GCCCGCGGGC AGGGCCGCGC GCACGCGCTG GCCCTGGCGG GCCAGGGGGC CGATATCGCC CTCGCCGACG CGCCGGGCCC GATGGCGGAG CTGACCTACC CGTTGGGCAG CGAGGAAGAC CTGCTCGCCA CCGCCGAACT CGTCGGCCAG CTGGGCCGGC GTTGCCTGCC GATGGTGGTC GACGTGCGCG ACGCGGCCCA GGTGAACACC GCGGTTGAGC GGACGGTGCG CGAGCTGGGC AGCCTCGATA TCGTGCTGGC CAATGCCGGC ATCGTCAGCA CCGGGCGCCT CGAAGAGGTA AGTGACCAGG TTTGGCAGCA GCTGATGGAC ACCAACCTGA CCGGCGTGTT CCACACCCTG CGCGCGGCCA TTCCGGTGAT GCGCCAACAA CGCTTCGGCA GGATCGTGGC GACATCGTCG ATGGGCGGCC GAATGGGCAT CCCGGAATTG GCGGCCTACA ACGCCACCAA GTGGGGGATC ATCGGCCTGA TCAAGTCCGT CGCCCTCGAG GTCGCCAAGG AAGGCATCAC GGCCAACGTC ATCTGTCCGA CCACGACGCA GACACCGATG GTGCAGCCCG CCGGGATCGG AGATGATCAG GAAGTTCCCG ACGATCTGGT GCGGCGCATG ATGAAGGCCA ACCCCATCCC TCAACCGTGG CTGCAACCGG AGGACGTCAG CCGCGGGGTG GTCTATCTGG TCACCGACCC GGGTGTCATC ACCGGCAGCG TGCTGGAAGT CGGGCTCGGC GGCAGCGCTC GCATGCACAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA