SSGCID: Target Page

MypaA.01326.m

Putative uncharacterized protein

CENTER ID:	MypaA.01326.m
ORGANISM:	Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE:	Johne's disease
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01326.m.A1.GE29594	Full length( MypaA.01326.m )		1	279

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MypaA.01326.m.A1.PW29494	Full length( MypaA.01326.m )		1	279

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|262316.17.peg.700
RefSeq:	NP_959599.1
UniProt:	Q743C0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGGRVEGKVA FITGAARGQG RSHAVRLAQE GADIIAVDIC APISSNSQIP PSTPDDLAET ADLIKGLDRR IVTAEVDVRD YYALKAAVDS GVEQLGRLDI ICANAGIGNG GQTLDKTSED DWRDMIDVNL SGVWKTVKAG VPHMISQGHG GSIILTSSVG GLKAYPHTGH YIAAKHGVVG LMRTFAVELG QHFIRVNSVH PTNVNTPMFM NEGTMRLFRP DLKNPGPDDL KVAAQFMHVL PVGWVEPVDI SNAVLFLASD ESRYITGLPV TVDAGSMLK

NT Sequence

ATGGGCGGAA GAGTCGAGGG CAAGGTCGCA TTCATCACCG GGGCGGCGCG GGGCCAGGGC CGCAGTCACG CGGTACGGCT GGCGCAGGAG GGCGCCGACA TCATCGCCGT CGACATCTGC GCCCCGATCA GCAGCAACAG CCAGATCCCG CCCTCGACGC CGGACGACCT GGCCGAGACG GCCGACCTCA TCAAGGGCCT CGACCGGCGC ATCGTGACCG CCGAGGTCGA CGTCCGTGAC TACTACGCGC TGAAAGCGGC GGTGGACAGC GGCGTCGAAC AGCTCGGACG GCTGGACATC ATCTGCGCCA ACGCCGGAAT CGGCAACGGC GGCCAGACCC TGGACAAAAC CAGCGAAGAC GACTGGCGAG ACATGATCGA CGTCAACCTT TCCGGTGTCT GGAAGACCGT CAAAGCCGGT GTGCCGCATA TGATTTCGCA AGGGCACGGC GGATCGATCA TCTTGACCAG CTCGGTCGGC GGACTCAAGG CCTACCCGCA CACCGGCCAC TACATCGCCG CCAAACACGG TGTCGTCGGG TTGATGCGGA CGTTCGCCGT CGAGCTCGGC CAGCACTTCA TCCGGGTGAA TTCCGTGCAC CCCACCAACG TGAACACACC GATGTTCATG AACGAGGGGA CGATGAGGCT GTTCCGGCCG GACCTGAAGA ACCCCGGCCC GGACGACCTG AAGGTCGCCG CGCAGTTCAT GCATGTGCTG CCGGTCGGCT GGGTGGAGCC GGTGGACATC AGCAACGCCG TGCTGTTCCT GGCCTCCGAC GAATCGCGTT ACATCACAGG TCTTCCGGTC ACCGTCGACG CCGGCAGCAT GCTCAAG

Details for MypaA.01326.m.A1.GE29594

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ttattacttg agcatgctgc cggcgtcgac ggtgaccgga agacctgtga tgtaacgcga ttcgtcggag gccaggaaca gcacggcgtt gctgatgtcc accggctcca cccagccgac cggcagcaca tgcatgaact gcgcggcgac cttcaggtcg tccgggccgg ggttcttcag gtccggccgg aacagcctca tcgtcccctc gttcatgaac atcggtgtgt tcacgttggt ggggtgcacg gaattcaccc ggatgaagtg ctggccgagc tcgacggcga acgtccgcat caacccgacg acaccgtgtt tggcggcgat gtagtggccg gtgtgcgggt aggccttgag tccgccgacc gagctggtca agatgatcga tccgccgtgc ccttgcgaaa tcatatgcgg cacaccggct ttgacggtct tccagacacc ggaaaggttg acgtcgatca tgtctcgcca gtcgtcttcg ctggttttgt ccagggtctg gccgccgttg ccgattccgg cgttggcgca gatgatgtcc agccgtccga gctgttcgac gccgctgtcc accgccgctt tcagcgcgta gtagtcacgg acgtcgacct cggcggtcac gatgcgccgg tcgaggccct tgatgaggtc ggccgtctcg gccaggtcgt ccggcgtcga gggcgggatc tggctgttgc tgctgatcgg ggcgcagatg tcgacggcga tgatgtcggc gccctcctgc gccagccgta ccgcgtgact gcggccctgg ccccgcgccg ccccggtgat gaatgcgacc ttgccctcga ctcttccgcc catcgaacca ggaccctggg tctgagcttc cagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGGRVEGKV AFITGAARGQ GRSHAVRLAQ EGADIIAVDI CAPISSNSQI PPSTPDDLAE TADLIKGLDR RIVTAEVDVR DYYALKAAVD SGVEQLGRLD IICANAGIGN GGQTLDKTSE DDWRDMIDVN LSGVWKTVKA GVPHMISQGH GGSIILTSSV GGLKAYPHTG HYIAAKHGVV GLMRTFAVEL GQHFIRVNSV HPTNVNTPMF MNEGTMRLFR PDLKNPGPDD LKVAAQFMHV LPVGWVEPVD ISNAVLFLAS DESRYITGLP VTVDAGSMLK

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcggaaga gtcgagggca aggtcgcatt catcaccggg gcggcgcggg gccagggccg cagtcacgcg gtacggctgg cgcaggaggg cgccgacatc atcgccgtcg acatctgcgc cccgatcagc agcaacagcc agatcccgcc ctcgacgccg gacgacctgg ccgagacggc cgacctcatc aagggcctcg accggcgcat cgtgaccgcc gaggtcgacg tccgtgacta ctacgcgctg aaagcggcgg tggacagcgg cgtcgaacag ctcggacggc tggacatcat ctgcgccaac gccggaatcg gcaacggcgg ccagaccctg gacaaaacca gcgaagacga ctggcgagac atgatcgacg tcaacctttc cggtgtctgg aagaccgtca aagccggtgt gccgcatatg atttcgcaag ggcacggcgg atcgatcatc ttgaccagct cggtcggcgg actcaaggcc tacccgcaca ccggccacta catcgccgcc aaacacggtg tcgtcgggtt gatgcggacg ttcgccgtcg agctcggcca gcacttcatc cgggtgaatt ccgtgcaccc caccaacgtg aacacaccga tgttcatgaa cgaggggacg atgaggctgt tccggccgga cctgaagaac cccggcccgg acgacctgaa ggtcgccgcg cagttcatgc atgtgctgcc ggtcggctgg gtggagccgg tggacatcag caacgccgtg ctgttcctgg cctccgacga atcgcgttac atcacaggtc ttccggtcac cgtcgacgcc ggcagcatgc tcaagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MypaA.01326.m.A1.PW29494

PURIFICATION DATe:	8/30/2010
CONCENTRATION:	24.9mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	data unavailable
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGGAAGA GTCGAGGGCA AGGTCGCATT CATCACCGGG GCGGCGCGGG GCCAGGGCCG CAGTCACGCG GTACGGCTGG CGCAGGAGGG CGCCGACATC ATCGCCGTCG ACATCTGCGC CCCGATCAGC AGCAACAGCC AGATCCCGCC CTCGACGCCG GACGACCTGG CCGAGACGGC CGACCTCATC AAGGGCCTCG ACCGGCGCAT CGTGACCGCC GAGGTCGACG TCCGTGACTA CTACGCGCTG AAAGCGGCGG TGGACAGCGG CGTCGAACAG CTCGGACGGC TGGACATCAT CTGCGCCAAC GCCGGAATCG GCAACGGCGG CCAGACCCTG GACAAAACCA GCGAAGACGA CTGGCGAGAC ATGATCGACG TCAACCTTTC CGGTGTCTGG AAGACCGTCA AAGCCGGTGT GCCGCATATG ATTTCGCAAG GGCACGGCGG ATCGATCATC TTGACCAGCT CGGTCGGCGG ACTCAAGGCC TACCCGCACA CCGGCCACTA CATCGCCGCC AAACACGGTG TCGTCGGGTT GATGCGGACG TTCGCCGTCG AGCTCGGCCA GCACTTCATC CGGGTGAATT CCGTGCACCC CACCAACGTG AACACACCGA TGTTCATGAA CGAGGGGACG ATGAGGCTGT TCCGGCCGGA CCTGAAGAAC CCCGGCCCGG ACGACCTGAA GGTCGCCGCG CAGTTCATGC ATGTGCTGCC GGTCGGCTGG GTGGAGCCGG TGGACATCAG CAACGCCGTG CTGTTCCTGG CCTCCGACGA ATCGCGTTAC ATCACAGGTC TTCCGGTCAC CGTCGACGCC GGCAGCATGC TCAAGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA