MypaA.01326.a

Putative uncharacterized protein

CENTER ID: MypaA.01326.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01326.a.A1.PW29470 Full length( MypaA.01326.a ) 1 273

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.4557
RefSeq: NP_963216.1
UniProt: Q73RZ6

Orthologues for Rv0687

Species % Identity % Coverage Clones Proteins Structures
bovis 100 100 1 1 0
marinum 82 100 1 1 0
avium 82 96 0 0 0
paratuberculosis 81 100 1 0 1
ulcerans 80 90 1 0 0
smegmatis 74 100 0 1 0
abscessus 63 97 1 1 0
avium 56 98 0 0 0
abscessus 55 97 1 1 0
thermoresistibile 51 77 0 0 0
avium 49 98 1 1 0
paratuberculosis 48 98 1 1 1
paratuberculosis 48 98 1 1 0
smegmatis 48 97 0 2 0
avium 47 98 1 0 0
smegmatis 47 97 0 1 0
avium 44 95 0 0 0
thermoresistibile 44 92 1 1 1
paratuberculosis 43 96 0 1 0
paratuberculosis 42 95 1 1 0
avium 42 90 0 0 0
abscessus 39 96 1 1 1
marinum 38 97 1 1 0
ulcerans 38 97 0 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQSLTDKVA LITGAARGQG RAHAARLAAE GADIIAVDLA GPLPPSVPYD SSTPEDLAET AELVRAAGRR VVTAQTDVRD LDALTSSVDT AVGELGRLDV IVANAGICSP APWNRITAQA FRDTIDTNVV GTWNTVMAGA QHIIDGGRGG SIILIGSAAG INMQSFMVHY TASKHAVVGM ARAFAAELGR YNIRVNSLNP GAVATPMGTG RMRDALRAAA DDYPHLRGLH KPLLPEGIAQ PEDIADAVAW LASDQSRLVT ASQVSVDLGV GYV
NT Sequence
ATGAGCCAGT CACTTACGGA CAAGGTCGCG CTGATCACCG GGGCGGCGCG CGGGCAGGGC CGCGCGCACG CGGCGCGGCT GGCCGCCGAG GGCGCCGACA TCATCGCCGT CGACCTCGCC GGGCCGCTGC CGCCGAGCGT TCCCTACGAC TCGTCGACGC CCGAGGACCT GGCCGAGACC GCGGAACTGG TGCGTGCCGC CGGACGGCGG GTCGTCACCG CACAGACCGA CGTGCGCGAC CTGGACGCCC TGACCTCCTC GGTGGACACG GCGGTGGGCG AGCTCGGCCG CCTGGACGTC ATCGTGGCCA ACGCCGGAAT CTGCAGCCCC GCCCCGTGGA ATCGGATCAC CGCGCAGGCG TTCCGGGACA CCATCGACAC CAACGTCGTC GGCACCTGGA ACACCGTGAT GGCGGGGGCG CAGCACATCA TCGACGGCGG CCGCGGCGGG TCGATCATCC TGATCGGCTC GGCGGCCGGC ATCAACATGC AGTCGTTCAT GGTCCACTAC ACCGCCAGCA AGCACGCCGT GGTGGGCATG GCCCGCGCGT TCGCCGCCGA ACTCGGCCGC TACAACATTC GGGTCAACAG CCTCAACCCC GGGGCGGTCG CCACCCCGAT GGGCACCGGC CGGATGCGCG ACGCGCTGCG CGCGGCCGCC GACGACTACC CGCATCTGCG GGGCCTGCAC AAACCGCTGC TGCCCGAGGG CATCGCGCAA CCGGAGGACA TCGCCGACGC GGTGGCCTGG CTGGCCTCCG ACCAATCCAG ATTGGTGACC GCCAGCCAGG TTTCGGTCGA TCTCGGCGTC GGCTACGTG
Details for MypaA.01326.a.A1.PW29470
PURIFICATION DATe: 2/4/2011
CONCENTRATION: 20.85mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 50µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMSQSLTD KVALITGAAR GQGRAHAARL AAEGADIIAV DLAGPLPPSV PYDSSTPEDL AETAELVRAA GRRVVTAQTD VRDLDALTSS VDTAVGELGR LDVIVANAGI CSPAPWNRIT AQAFRDTIDT NVVGTWNTVM AGAQHIIDGG RGGSIILIGS AAGINMQSFM VHYTASKHAV VGMARAFAAE LGRYNIRVNS LNPGAVATPM GTGRMRDALR AAADDYPHLR GLHKPLLPEG IAQPEDIADA VAWLASDQSR LVTASQVSVD LGVG
Validated NT Sequence
agccgacgcc gagatcgacc gaaacctggc tggcggtcac caatctggat tggtcggagg ccagccaggc caccgcgtcg gcgatgtcct ccggttgcgc gatgccctcg ggcagcagcg gtttgtgcag gccccgcaga tgcgggtagt cgtcggcggc cgcgcgcagc gcgtcgcgca tccggccggt gcccatcggg gtggcgaccg ccccggggtt gaggctgttg acccgaatgt tgtagcggcc gagttcggcg gcgaacgcgc gggccatgcc caccacggcg tgcttgctgg cggtgtagtg gaccatgaac gactgcatgt tgatgccggc cgccgagccg atcaggatga tcgacccgcc gcggccgccg tcgatgatgt gctgcgcccc cgccatcacg gtgttccagg tgccgacgac gttggtgtcg atggtgtccc ggaacgcctg cgcggtgatc cgattccacg gggcggggct gcagattccg gcgttggcca cgatgacgtc caggcggccg agctcgccca ccgccgtgtc caccgaggag gtcagggcgt ccaggtcgcg cacgtcggtc tgtgcggtga cgacccgccg tccggcggca cgcaccagtt ccgcggtctc ggccaggtcc tcgggcgtcg acgagtcgta gggaacgctc ggcggcagcg gcccggcgag gtcgacggcg atgatgtcgg cgccctcggc ggccagccgc gccgcgtgcg cgcggccctg cccgcgcgcc gccccggtga tcagcgcgac cttgtccgta agtgactggc tcatcgaacc aggaccctgg gtctgagctt ccagggtacc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQSLTDKV ALITGAARGQ GRAHAARLAA EGADIIAVDL AGPLPPSVPY DSSTPEDLAE TAELVRAAGR RVVTAQTDVR DLDALTSSVD TAVGELGRLD VIVANAGICS PAPWNRITAQ AFRDTIDTNV VGTWNTVMAG AQHIIDGGRG GSIILIGSAA GINMQSFMVH YTASKHAVVG MARAFAAELG RYNIRVNSLN PGAVATPMGT GRMRDALRAA ADDYPHLRGL HKPLLPEGIA QPEDIADAVA WLASDQSRLV TASQVSVDLG VGYV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCCAGTCA CTTACGGACA AGGTCGCGCT GATCACCGGG GCGGCGCGCG GGCAGGGCCG CGCGCACGCG GCGCGGCTGG CCGCCGAGGG CGCCGACATC ATCGCCGTCG ACCTCGCCGG GCCGCTGCCG CCGAGCGTTC CCTACGACTC GTCGACGCCC GAGGACCTGG CCGAGACCGC GGAACTGGTG CGTGCCGCCG GACGGCGGGT CGTCACCGCA CAGACCGACG TGCGCGACCT GGACGCCCTG ACCTCCTCGG TGGACACGGC GGTGGGCGAG CTCGGCCGCC TGGACGTCAT CGTGGCCAAC GCCGGAATCT GCAGCCCCGC CCCGTGGAAT CGGATCACCG CGCAGGCGTT CCGGGACACC ATCGACACCA ACGTCGTCGG CACCTGGAAC ACCGTGATGG CGGGGGCGCA GCACATCATC GACGGCGGCC GCGGCGGGTC GATCATCCTG ATCGGCTCGG CGGCCGGCAT CAACATGCAG TCGTTCATGG TCCACTACAC CGCCAGCAAG CACGCCGTGG TGGGCATGGC CCGCGCGTTC GCCGCCGAAC TCGGCCGCTA CAACATTCGG GTCAACAGCC TCAACCCCGG GGCGGTCGCC ACCCCGATGG GCACCGGCCG GATGCGCGAC GCGCTGCGCG CGGCCGCCGA CGACTACCCG CATCTGCGGG GCCTGCACAA ACCGCTGCTG CCCGAGGGCA TCGCGCAACC GGAGGACATC GCCGACGCGG TGGCCTGGCT GGCCTCCGAC CAATCCAGAT TGGTGACCGC CAGCCAGGTT TCGGTCGATC TCGGCGTCGG CTACGTGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA