MysmA.01326.f

Carveol dehydrogenase

CENTER ID: MysmA.01326.f
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01326.f.A1.PS00651 Full length( MysmA.01326.f ) 1 283

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.1397
RefSeq: YP_885793.1
UniProt: A0QSA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATVGKTLEG RVAFVTGAAR GQGRAHAVRL ADEGADIIAI DVCAPVDDTI TYPSATPEDL AETVAAVEAT GRKVLAREVD IRDLAAQQQV VADGVEQFGR LDIVVANAGV LSWGRIFEMS PEQWDTVVDV NLNGTWRTIR AAVPAMIEAG NGGSIIIVSS SAGLKATPGN GHYAASKHGL VALTNSLALE VGEYGIRVNS IHPYSIDTPM IEPEAMAQVF AKYPNYLHSF APMPYQPVGE GAEGLSTFMT PEEVSDVVAW LAGDGSATLS GSQIAVDRGV MKY
NT Sequence
ATGGCGACAG TTGGTAAAAC GCTGGAAGGA CGCGTGGCGT TCGTTACCGG GGCGGCTCGC GGCCAGGGAC GGGCGCATGC CGTGCGACTG GCCGACGAGG GCGCCGACAT CATCGCCATC GACGTGTGCG CACCCGTCGA CGACACCATC ACCTATCCGT CGGCGACACC GGAGGATCTC GCCGAGACCG TCGCCGCGGT CGAGGCGACC GGGCGCAAGG TCCTCGCGCG CGAGGTCGAC ATCCGTGACC TCGCGGCCCA GCAGCAGGTG GTCGCCGACG GTGTCGAGCA GTTCGGCCGG CTCGACATCG TGGTGGCCAA CGCGGGCGTG CTCAGCTGGG GACGCATCTT CGAGATGTCG CCCGAACAGT GGGACACCGT CGTCGACGTC AACCTCAACG GCACGTGGCG CACCATCCGC GCGGCCGTGC CCGCGATGAT CGAGGCGGGC AACGGCGGGT CGATCATCAT CGTCAGCTCG TCGGCCGGGC TCAAGGCCAC GCCCGGCAAC GGGCACTACG CGGCCTCCAA GCACGGGCTC GTCGCGCTCA CCAACTCGCT GGCTCTCGAG GTGGGGGAGT ACGGGATCCG CGTCAACTCC ATCCACCCCT ACTCGATCGA CACGCCCATG ATCGAGCCCG AGGCCATGGC GCAGGTGTTC GCGAAGTACC CGAATTACCT GCACAGCTTC GCGCCCATGC CGTACCAGCC GGTCGGCGAA GGCGCCGAAG GGCTTTCGAC GTTCATGACC CCCGAAGAGG TGTCCGACGT GGTGGCCTGG CTCGCCGGCG ACGGCTCGGC GACCTTGTCG GGCAGCCAGA TCGCCGTCGA CCGCGGCGTC ATGAAGTAC
Details for MysmA.01326.f.A1.PS00651
PURIFICATION DATe: 7/1/2010
CONCENTRATION: 58.18mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MATVGKTLEG RVAFVTGAAR GQGRAHAVRL ADEGADIIAI DVCAPVDDTI TYPSATPEDL AETVAAVEAT GRKVLAREVD IRDLAAQQQV VADGVEQFGR LDIVVANAGV LSWGRIFEMS PEQWDTVVDV NLNGTWRTIR AAVPAMIEAG NGGSIIIVSS SAGLKATPGN GHYAASKHGL VALTNSLALE VGEYGIRVNS IHPYSIDTPM IEPEAMAQVF AKYPNYLHSF APMPYQPVGE GAEGLSTFMT PEEVSDVVAW LAGDGSATLS GSQIAVDRGV MKY
Validated NT Sequence
ttcgngcttt gttagcagcc ggatcctcga gaagcttggc tgcagaactt gttcgtgctg tttattagta cttcatgacg ccgcggtcga cggcgatctg gctgcccgac aaggtcgccg agccgtcgcc ggcgagccag gccaccacgt cggacacctc ttcgggggtc atgaacgtcg aaagcccttc ggcgccttcg ccgaccggct ggtacggcat gggcgcgaag ctgtgcaggt aattcgggta cttcgcgaac acctgcgcca tggcctcggg ctcgatcatg ggcgtgtcga tcgagtaggg gtggatggag ttgacgcgga tcccgtactc ccccacctcg agagccagcg agttggtgag cgcgacgagc ccgtgcttgg aggccgcgta gtgcccgttg ccgggcgtgg ccttgagccc ggccgacgag ctgacgatga tgatcgaccc gccgttgccc gcctcgatca tcgcgggcac ggccgcgcgg atggtgcgcc acgtgccgtt gaggttgacg tcgacgacgg tgtcccactg ttcgggcgac atctcgaaga tgcgtcccca gctgagcacg cccgcgttgg ccaccacgat gtcgagccgg ccgaactgct cgacaccgtc ggcgaccacc tgctgctggg ccgcgaggtc acggatgtcg acctcgcgcg cgaggacctt gcgcccggtc gcctcgaccg cggcgacggt ctcggcgaga tcctccggtg tcgccgacgg ataggtgatg gtgtcgtcga cgggtgcgca cacgtcgatg gcgatgatgt cggcgccctc gtcggccagt cgcacggcat gcgcccgtcc ctggccgcga gccgccccgg taacgaacgc cacgcgtcct tccagcgttt taccaactgt cgccatcgaa ccngnaccct gggtctgagc ttccnggnac ccatatgatg gtgatggtga tgagccatgn ntntctcntc tnaagtnana aatnnttnct aganggnann cgtnnnnnnn nnnnngnngn nnnnnnnnnn nnnnnnnnnn a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATVGKTLE GRVAFVTGAA RGQGRAHAVR LADEGADIIA IDVCAPVDDT ITYPSATPED LAETVAAVEA TGRKVLAREV DIRDLAAQQQ VVADGVEQFG RLDIVVANAG VLSWGRIFEM SPEQWDTVVD VNLNGTWRTI RAAVPAMIEA GNGGSIIIVS SSAGLKATPG NGHYAASKHG LVALTNSLAL EVGEYGIRVN SIHPYSIDTP MIEPEAMAQV FAKYPNYLHS FAPMPYQPVG EGAEGLSTFM TPEEVSDVVA WLAGDGSATL SGSQIAVDRG VMKY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGACAGTT GGTAAAACGC TGGAAGGACG CGTGGCGTTC GTTACCGGGG CGGCTCGCGG CCAGGGACGG GCGCATGCCG TGCGACTGGC CGACGAGGGC GCCGACATCA TCGCCATCGA CGTGTGCGCA CCCGTCGACG ACACCATCAC CTATCCGTCG GCGACACCGG AGGATCTCGC CGAGACCGTC GCCGCGGTCG AGGCGACCGG GCGCAAGGTC CTCGCGCGCG AGGTCGACAT CCGTGACCTC GCGGCCCAGC AGCAGGTGGT CGCCGACGGT GTCGAGCAGT TCGGCCGGCT CGACATCGTG GTGGCCAACG CGGGCGTGCT CAGCTGGGGA CGCATCTTCG AGATGTCGCC CGAACAGTGG GACACCGTCG TCGACGTCAA CCTCAACGGC ACGTGGCGCA CCATCCGCGC GGCCGTGCCC GCGATGATCG AGGCGGGCAA CGGCGGGTCG ATCATCATCG TCAGCTCGTC GGCCGGGCTC AAGGCCACGC CCGGCAACGG GCACTACGCG GCCTCCAAGC ACGGGCTCGT CGCGCTCACC AACTCGCTGG CTCTCGAGGT GGGGGAGTAC GGGATCCGCG TCAACTCCAT CCACCCCTAC TCGATCGACA CGCCCATGAT CGAGCCCGAG GCCATGGCGC AGGTGTTCGC GAAGTACCCG AATTACCTGC ACAGCTTCGC GCCCATGCCG TACCAGCCGG TCGGCGAAGG CGCCGAAGGG CTTTCGACGT TCATGACCCC CGAAGAGGTG TCCGACGTGG TGGCCTGGCT CGCCGGCGAC GGCTCGGCGA CCTTGTCGGG CAGCCAGATC GCCGTCGACC GCGGCGTCAT GAAGTACAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA