MysmA.01326.d

Carveol dehydrogenase

CENTER ID: MysmA.01326.d
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01326.d.A1.PS00645 Full length( MysmA.01326.d ) 1 279

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5869
RefSeq: YP_890256.1
UniProt: A0R518

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGRVEGKVA FITGAARGQG RSHAVRLAEE GADIIAVDVC RRISSNEDIP ASTPEDLAET VELVKGLNRR IVAEEVDVRD YDALKAVVDS GVEQLGGLDI VVANAGIGNG GATLDKTSEA DWDDMIGVNL SGVWKTVKAA VPHLISGGNG GSIILTSSVG GLKAYPHTGH YIAAKHGVVG LMRTFAVELG QHSIRVNSVH PTNVNTPLFM NEGTMKLFRP DLENPGPDDM AVVAQMMHVL PVGWVEPRDI SNAVLFLASD EARYVTGLPM TVDAGSMLK
NT Sequence
ATGGCAGGCA GGGTCGAGGG CAAGGTCGCT TTCATCACGG GTGCGGCGCG CGGGCAGGGC CGCAGTCACG CCGTGCGGCT GGCTGAGGAG GGCGCCGACA TCATCGCGGT CGACGTCTGC CGACGGATCA GCTCCAATGA GGACATCCCG GCGTCGACAC CCGAAGACCT GGCGGAAACC GTCGAACTGG TCAAAGGCCT CAATCGCCGG ATCGTCGCCG AGGAAGTCGA CGTGCGTGAC TACGACGCGC TCAAGGCCGT CGTGGACAGC GGCGTCGAAC AGCTCGGCGG CCTCGACATC GTGGTCGCCA ACGCCGGCAT CGGCAACGGC GGTGCGACGC TGGACAAGAC CAGCGAGGCC GATTGGGACG ACATGATCGG CGTGAACCTG TCCGGCGTCT GGAAGACCGT GAAAGCCGCT GTGCCGCACC TGATCTCCGG CGGCAACGGC GGGTCGATCA TCCTCACCAG CTCGGTGGGC GGACTCAAGG CCTATCCGCA CACGGGCCAC TACATCGCCG CCAAGCACGG CGTGGTGGGC CTGATGCGGA CCTTCGCGGT CGAACTGGGG CAGCACTCGA TCCGGGTGAA CTCGGTGCAC CCCACGAACG TCAACACGCC GCTGTTCATG AACGAGGGCA CCATGAAACT GTTCCGTCCG GATCTGGAGA ACCCGGGTCC CGACGACATG GCCGTCGTGG CGCAGATGAT GCACGTCCTG CCGGTCGGCT GGGTCGAGCC GCGCGACATC AGCAACGCGG TGCTGTTCCT GGCATCCGAC GAGGCGCGCT ATGTGACGGG TCTGCCGATG ACGGTCGACG CGGGGAGCAT GCTCAAG
Details for MysmA.01326.d.A1.PS00645
PURIFICATION DATe: 6/24/2010
CONCENTRATION: 64.16mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG GSSDPGXGSM AGRVEGKVAF ITGAARGQGR SHAVRLAEEG ADIIAVDVCR RISSNEDIPA STPEDLAETV ELVKGLNRRI VAEEVDVRDY DALKAVVDSG VEQLGGLDIV VANAGIGNGG ATLDKTSEAD WDDMIGVNLS GVWKTVKAAV PHLISGGNGG SIILTSSVGG LKAYPHTGHY IAAKHGVVGL MRTFAVELGQ HSIRVNSVHP TNVNTPLFMN EGTMKLFRPD LENPGPDDMA VVAQMMHVLP VGWVEPRDIS NAVLFLASDE ARYVTGLPMT VDAGSMLK
Validated NT Sequence
cnnnnttcgg gctttgttag cagccggatc ctcgagaagc ttggctgcag aacttgttcg tgctgtttat tacttgagca tgctccccgc gtcgaccgtc atcggcagac ccgtcacata gcgcgcctcg tcggatgcca ggaacagcac cgcgttgctg atgtcgcgcg gctcgaccca gccgaccggc aggacgtgca tcatctgcgc cacgacggcc atgtcgtcgg gacccgggtt ctccagatcc ggacggaaca gtttcatggt gccctcgttc atgaacagcg gcgtgttgac gttcgtgggg tgcaccgagt tcacccggat cgagtgctgc cccagttcga ccgcgaaggt ccgcatcagg cccaccacgc cgtgcttggc ggcgatgtag tggcccgtgt gcggataggc cttgagtccg cccaccgagc tggtgaggat gatcgacccg ccgttgccgc cggagatcag gtgcggcaca gcggctttca cggtcttcca gacgccggac aggttcacgc cgatcatgtc gtcccaatcg gcctcgctgg tcttgtccag cgtcgcaccg ccgttgccga tgccggcgtt ggcgaccacg atgtcgaggc cgccgagctg ttcgacgccg ctgtccacga cggccttgag cgcgtcgtag tcacgcacgt cgacttcctc ggcgacgatc cggcgattga ggcctttgac cagttcgacg gtttccgcca ggtcttcggg tgtcgacgcc gggatgtcct cattggagct gatccgtcgg cagacgtcga ccgcgatgat gtcggcgccc tcctcagcca gccgcacggc gtgactgcgg ccctgcccgc gcgccgcacc cgtgatgaaa gcgaccttgc cctcgaccct gcctgccatc gaaccnnncc ctgggtctga gcttccaccc atatgatggt gatggtgatg agccatgnnt atctnnnnna nantnatttn tagagggaan ngtnng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAGRVEGKV AFITGAARGQ GRSHAVRLAE EGADIIAVDV CRRISSNEDI PASTPEDLAE TVELVKGLNR RIVAEEVDVR DYDALKAVVD SGVEQLGGLD IVVANAGIGN GGATLDKTSE ADWDDMIGVN LSGVWKTVKA AVPHLISGGN GGSIILTSSV GGLKAYPHTG HYIAAKHGVV GLMRTFAVEL GQHSIRVNSV HPTNVNTPLF MNEGTMKLFR PDLENPGPDD MAVVAQMMHV LPVGWVEPRD ISNAVLFLAS DEARYVTGLP MTVDAGSMLK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCAGGCAGG GTCGAGGGCA AGGTCGCTTT CATCACGGGT GCGGCGCGCG GGCAGGGCCG CAGTCACGCC GTGCGGCTGG CTGAGGAGGG CGCCGACATC ATCGCGGTCG ACGTCTGCCG ACGGATCAGC TCCAATGAGG ACATCCCGGC GTCGACACCC GAAGACCTGG CGGAAACCGT CGAACTGGTC AAAGGCCTCA ATCGCCGGAT CGTCGCCGAG GAAGTCGACG TGCGTGACTA CGACGCGCTC AAGGCCGTCG TGGACAGCGG CGTCGAACAG CTCGGCGGCC TCGACATCGT GGTCGCCAAC GCCGGCATCG GCAACGGCGG TGCGACGCTG GACAAGACCA GCGAGGCCGA TTGGGACGAC ATGATCGGCG TGAACCTGTC CGGCGTCTGG AAGACCGTGA AAGCCGCTGT GCCGCACCTG ATCTCCGGCG GCAACGGCGG GTCGATCATC CTCACCAGCT CGGTGGGCGG ACTCAAGGCC TATCCGCACA CGGGCCACTA CATCGCCGCC AAGCACGGCG TGGTGGGCCT GATGCGGACC TTCGCGGTCG AACTGGGGCA GCACTCGATC CGGGTGAACT CGGTGCACCC CACGAACGTC AACACGCCGC TGTTCATGAA CGAGGGCACC ATGAAACTGT TCCGTCCGGA TCTGGAGAAC CCGGGTCCCG ACGACATGGC CGTCGTGGCG CAGATGATGC ACGTCCTGCC GGTCGGCTGG GTCGAGCCGC GCGACATCAG CAACGCGGTG CTGTTCCTGG CATCCGACGA GGCGCGCTAT GTGACGGGTC TGCCGATGAC GGTCGACGCG GGGAGCATGC TCAAGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA