MyabA.01326.f
Putative short-chain dehydrogenase/reductase
| CENTER ID: | MyabA.01326.f |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01326.f.A1.GE30501 | Full length( MyabA.01326.f ) | 1 | 277 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01326.f.A1.PW30378 | Full length( MyabA.01326.f ) | 1 | 277 |
Structures
3S55
DEPOSITED: 5/20/2011
DETERMINATION: XRay
CLONE: MyabA.01326.f.A1.GE30501
PROTEIN: MyabA.01326.f.A1.PW30378
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|36809.5.peg.1446 |
| RefSeq: | YP_001702148.1 |
| UniProt: | B1MLR7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADFEGKTAL ITGGARGMGR SHAVALAEAG ADIAICDRCE NSDVVGYPLA TADDLAETVA LVEKTGRRCI SAKVDVKDRA ALESFVAEAE DTLGGIDIAI TNAGISTIAL LPEVESAQWD EVIGTNLTGT FNTIAAVAPG MIKRNYGRIV TVSSMLGHSA NFAQASYVSS KWGVIGLTKC AAHDLVGYGI TVNAVAPGNI ETPMTHNDFV FGTMRPDLEK PTLKDVESVF ASLHLQYAPF LKPEEVTRAV LFLVDEASSH ITGTVLPIDA GATARMI
NT Sequence
GTGGCTGATT TCGAGGGAAA AACTGCACTG ATCACGGGCG GCGCGCGCGG TATGGGCCGG TCGCACGCGG TGGCGCTGGC CGAGGCCGGC GCCGATATCG CGATCTGTGA TCGCTGTGAG AACAGCGATG TCGTGGGATA TCCGCTGGCA ACCGCAGACG ACCTCGCCGA GACGGTCGCA TTGGTCGAAA AGACCGGACG CCGGTGCATC TCGGCCAAGG TGGACGTGAA GGACCGGGCC GCACTGGAAT CGTTTGTGGC CGAGGCCGAG GACACGCTCG GCGGTATCGA CATCGCCATC ACCAACGCCG GGATCAGCAC CATCGCGCTG CTGCCCGAGG TCGAATCCGC GCAGTGGGAC GAGGTTATCG GCACTAACCT CACCGGAACC TTCAATACCA TCGCGGCCGT CGCGCCAGGA ATGATCAAAC GAAACTACGG CCGCATCGTG ACCGTGTCCT CGATGCTCGG CCACAGCGCG AACTTTGCGC AGGCCTCCTA CGTGTCGTCC AAATGGGGCG TGATCGGCCT CACCAAATGC GCGGCCCACG ATCTCGTCGG CTACGGGATC ACTGTCAACG CCGTCGCTCC GGGCAATATC GAAACCCCGA TGACGCACAA TGATTTCGTA TTCGGCACGA TGCGGCCCGA TCTGGAGAAA CCGACGCTCA AGGACGTGGA GTCCGTGTTC GCGTCCCTGC ATCTGCAATA TGCCCCCTTC CTCAAACCCG AAGAGGTCAC CCGCGCCGTG CTGTTCCTGG TCGACGAGGC GAGCTCACAT ATCACCGGTA CCGTGTTGCC GATCGACGCC GGCGCCACGG CGCGAATGAT A
Details for MyabA.01326.f.A1.GE30501
| HARVESTED ON: | 9/21/2010 |
| SEQUENCED ON: | 7/12/2017 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVADFEGKT ALITGGARGM GRSHAVALAE AGADIAICDR CENSDVVGYP LATADDLAET VALVEKTGRR CISAKVDVKD RAALESFVAE AEDTLGGIDI AITNAGISTI ALLPEVESAQ WDEVIGTNLT GTFNTIAAVA PGMIKRNYGR IVTVSSMLGH SANFAQASYV SSKWGVIGLT KCAAHDLVGY GITVNAVAPG NIETPMTHND FVFGTMRPDL EKPTLKDVES VFASLHLQYA PFLKPEEVTR AVLFLVDEAS SHITGTVLPI DAGATARMI
Validated NT Sequence
ttattatatc attcgcgccg tggcgccggc gtcgatcggc aacacggtac cggtgatatg tgagctcgcc tcgtcgacca ggaacagcac ggcgcgggtg acctcttcgg gtttgaggaa gggggcatat tgcagatgca gggacgcgaa cacggactcc acgtccttga gcgtcggttt ctccagatcg ggccgcatcg tgccgaatac gaaatcattg tgcgtcatcg gggtttcgat attgcccgga gcgacggcgt tgacagtgat cccgtagccg acgagatcgt gggccgcgca tttggtgagg ccgatcacgc cccatttgga cgacacgtag gaggcctgcg caaagttcgc gctgtggccg agcatcgagg acacggtcac gatgcggccg tagtttcgtt tgatcattcc tggcgcgacg gccgcgatgg tattgaaggt tccggtgagg ttagtgccga taacctcgtc ccactgcgcg gattcgacct cgggcagcag cgcgatggtg ctgatcccgg cgttggtgat ggcgatgtcg ataccgccga gcgtgtcctc ggcctcggcc acaaacgatt ccagtgcggc ccggtccttc acgtccacct tggccgagat gcaccggcgt ccggtctttt cgaccaatgc gaccgtctcg gcgaggtcgt ctgcggttgc cagcggatat cccacgacat cgctgttctc acagcgatca cagatcgcga tatcggcgcc ggcctcggcc agcgccaccg cgtgcgaccg gcccataccg cgcgcgccgc ccgtgatcag tgcagttttt ccctcgaaat cagccaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVADFEGKT ALITGGARGM GRSHAVALAE AGADIAICDR CENSDVVGYP LATADDLAET VALVEKTGRR CISAKVDVKD RAALESFVAE AEDTLGGIDI AITNAGISTI ALLPEVESAQ WDEVIGTNLT GTFNTIAAVA PGMIKRNYGR IVTVSSMLGH SANFAQASYV SSKWGVIGLT KCAAHDLVGY GITVNAVAPG NIETPMTHND FVFGTMRPDL EKPTLKDVES VFASLHLQYA PFLKPEEVTR AVLFLVDEAS SHITGTVLPI DAGATARMI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggctgat ttcgagggaa aaactgcact gatcacgggc ggcgcgcgcg gtatgggccg gtcgcacgcg gtggcgctgg ccgaggccgg cgccgatatc gcgatctgtg atcgctgtga gaacagcgat gtcgtgggat atccgctggc aaccgcagac gacctcgccg agacggtcgc attggtcgaa aagaccggac gccggtgcat ctcggccaag gtggacgtga aggaccgggc cgcactggaa tcgtttgtgg ccgaggccga ggacacgctc ggcggtatcg acatcgccat caccaacgcc gggatcagca ccatcgcgct gctgcccgag gtcgaatccg cgcagtggga cgaggttatc ggcactaacc tcaccggaac cttcaatacc atcgcggccg tcgcgccagg aatgatcaaa cgaaactacg gccgcatcgt gaccgtgtcc tcgatgctcg gccacagcgc gaactttgcg caggcctcct acgtgtcgtc caaatggggc gtgatcggcc tcaccaaatg cgcggcccac gatctcgtcg gctacgggat cactgtcaac gccgtcgctc cgggcaatat cgaaaccccg atgacgcaca atgatttcgt attcggcacg atgcggcccg atctggagaa accgacgctc aaggacgtgg agtccgtgtt cgcgtccctg catctgcaat atgccccctt cctcaaaccc gaagaggtca cccgcgccgt gctgttcctg gtcgacgagg cgagctcaca tatcaccggt accgtgttgc cgatcgacgc cggcgccacg gcgcgaatga tataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyabA.01326.f.A1.PW30378
| PURIFICATION DATe: | 12/6/2010 |
| CONCENTRATION: | 27.32mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVADFEGKT ALITGGARGM GRSHAVALAE AGADIAICDR CENSDVVGYP LATADDLAET VALVEKTGRR CISAKVDVKD RAALESFVAE AEDTLGGIDI AITNAGISTI ALLPEVESAQ WDEVIGTNLT GTFNTIAAVA PGMIKRNYGR IVTVSSMLGH SANFAQASYV SSKWGVIGLT KCAAHDLVGY GITVNAVAPG NIETPMTHND FVFGTMRPDL EKPTLKDVES VFASLHLQYA PFLKPEEVTR AVLFLVDEAS SHITGTVLPI DAGATARMI
Validated NT Sequence
ttattatatc attcgcgccg tggcgccggc gtcgatcggc aacacggtac cggtgatatg tgagctcgcc tcgtcgacca ggaacagcac ggcgcgggtg acctcttcgg gtttgaggaa gggggcatat tgcagatgca gggacgcgaa cacggactcc acgtccttga gcgtcggttt ctccagatcg ggccgcatcg tgccgaatac gaaatcattg tgcgtcatcg gggtttcgat attgcccgga gcgacggcgt tgacagtgat cccgtagccg acgagatcgt gggccgcgca tttggtgagg ccgatcacgc cccatttgga cgacacgtag gaggcctgcg caaagttcgc gctgtggccg agcatcgagg acacggtcac gatgcggccg tagtttcgtt tgatcattcc tggcgcgacg gccgcgatgg tattgaaggt tccggtgagg ttagtgccga taacctcgtc ccactgcgcg gattcgacct cgggcagcag cgcgatggtg ctgatcccgg cgttggtgat ggcgatgtcg ataccgccga gcgtgtcctc ggcctcggcc acaaacgatt ccagtgcggc ccggtccttc acgtccacct tggccgagat gcaccggcgt ccggtctttt cgaccaatgc gaccgtctcg gcgaggtcgt ctgcggttgc cagcggatat cccacgacat cgctgttctc acagcgatca cagatcgcga tatcggcgcc ggcctcggcc agcgccaccg cgtgcgaccg gcccataccg cgcgcgccgc ccgtgatcag tgcagttttt ccctcgaaat cagccaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVADFEGKT ALITGGARGM GRSHAVALAE AGADIAICDR CENSDVVGYP LATADDLAET VALVEKTGRR CISAKVDVKD RAALESFVAE AEDTLGGIDI AITNAGISTI ALLPEVESAQ WDEVIGTNLT GTFNTIAAVA PGMIKRNYGR IVTVSSMLGH SANFAQASYV SSKWGVIGLT KCAAHDLVGY GITVNAVAPG NIETPMTHND FVFGTMRPDL EKPTLKDVES VFASLHLQYA PFLKPEEVTR AVLFLVDEAS SHITGTVLPI DAGATARMI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCTGAT TTCGAGGGAA AAACTGCACT GATCACGGGC GGCGCGCGCG GTATGGGCCG GTCGCACGCG GTGGCGCTGG CCGAGGCCGG CGCCGATATC GCGATCTGTG ATCGCTGTGA GAACAGCGAT GTCGTGGGAT ATCCGCTGGC AACCGCAGAC GACCTCGCCG AGACGGTCGC ATTGGTCGAA AAGACCGGAC GCCGGTGCAT CTCGGCCAAG GTGGACGTGA AGGACCGGGC CGCACTGGAA TCGTTTGTGG CCGAGGCCGA GGACACGCTC GGCGGTATCG ACATCGCCAT CACCAACGCC GGGATCAGCA CCATCGCGCT GCTGCCCGAG GTCGAATCCG CGCAGTGGGA CGAGGTTATC GGCACTAACC TCACCGGAAC CTTCAATACC ATCGCGGCCG TCGCGCCAGG AATGATCAAA CGAAACTACG GCCGCATCGT GACCGTGTCC TCGATGCTCG GCCACAGCGC GAACTTTGCG CAGGCCTCCT ACGTGTCGTC CAAATGGGGC GTGATCGGCC TCACCAAATG CGCGGCCCAC GATCTCGTCG GCTACGGGAT CACTGTCAAC GCCGTCGCTC CGGGCAATAT CGAAACCCCG ATGACGCACA ATGATTTCGT ATTCGGCACG ATGCGGCCCG ATCTGGAGAA ACCGACGCTC AAGGACGTGG AGTCCGTGTT CGCGTCCCTG CATCTGCAAT ATGCCCCCTT CCTCAAACCC GAAGAGGTCA CCCGCGCCGT GCTGTTCCTG GTCGACGAGG CGAGCTCACA TATCACCGGT ACCGTGTTGC CGATCGACGC CGGCGCCACG GCGCGAATGA TATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA