MysmA.01326.g

Carveol dehydrogenase ((+)-trans-carveol dehydrogenase)

CENTER ID: MysmA.01326.g
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01326.g.A1.PS00653 Full length( MysmA.01326.g ) 1 273

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2171
RefSeq: YP_886558.1
UniProt: A0QUH0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAERLTGRVA FITGAARGQG RAHAVRMAKE GADIIAVDIA GPLPPSVPYD SATPEDLAET TRLVEATGRR ILAARADTRD LEALRDVVGK GVAEFGRLDV IVANAGITVP EPWNETTPES FRDVMDINVT GTWNTVMAGA QHIIDGGRGG SIILISSAAG IKMQPFMVHY TASKHAVAGM ARAFAAELGK HRIRVNSLHP GAVNTPMGTG DMLAALTRAN DTNPGLMQMV TPFLPDYIAE PEDIADAAVW LASDESRFVT ASRVAVDLGS TQF
NT Sequence
ATGGCCGAGC GGCTGACCGG ACGGGTCGCG TTCATCACCG GGGCCGCCCG CGGTCAGGGC CGCGCGCATG CGGTCCGCAT GGCCAAGGAG GGCGCCGACA TCATCGCGGT CGACATCGCC GGGCCTTTGC CACCCAGCGT GCCCTACGAT TCGGCGACGC CGGAGGATCT CGCCGAGACC ACCCGCCTGG TCGAGGCCAC GGGTCGGCGA ATCCTGGCCG CCAGGGCCGA CACTCGCGAC CTGGAGGCAC TGCGCGACGT CGTCGGCAAG GGTGTCGCCG AGTTCGGCAG GCTCGACGTC ATCGTCGCCA ACGCGGGTAT CACCGTCCCG GAACCGTGGA ACGAGACCAC GCCCGAGTCG TTCCGCGACG TCATGGACAT CAACGTCACC GGCACCTGGA ACACCGTGAT GGCCGGCGCC CAACACATCA TCGACGGGGG CCGCGGCGGT TCGATCATCC TGATCAGCTC CGCGGCGGGC ATCAAGATGC AGCCGTTCAT GGTGCACTAC ACCGCGAGCA AGCATGCTGT CGCAGGCATG GCCCGCGCGT TCGCCGCCGA GCTGGGCAAG CACCGGATCC GGGTCAACAG CCTGCATCCA GGTGCCGTCA ACACCCCGAT GGGTACCGGC GACATGCTGG CCGCCCTCAC GCGCGCCAAC GACACCAATC CGGGCCTGAT GCAGATGGTC ACGCCGTTCC TGCCGGACTA CATCGCCGAA CCCGAGGACA TCGCCGACGC CGCCGTGTGG CTGGCGAGCG ACGAATCGCG CTTCGTCACC GCGAGCCGGG TGGCTGTCGA CCTGGGTTCG ACGCAGTTC
Details for MysmA.01326.g.A1.PS00653
PURIFICATION DATe: 6/18/2010
CONCENTRATION: 73.2mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 96
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MGXXXSDPGX GSMAERLTGR VAFITGAARG QGRAHAVRMA KEGADIIAVD IAGPLPPSVP YDSATPEDLA ETTRLVEATG RRILAARADT RDLEALRDVV GKGVAEFGRL DVIVANAGIT VPEPWNETTP ESFRDVMDIN VTGTWNTVMA GAQHIIDGGR GGSIILISSA AGIKMQPFMV HYTASKHAVA GMARAFAAEL GKHRIRVNSL HPGAVNTPMG TGDMLAALTR ANDTNPGLMQ MVTPFLPDYI AEPEDIADAA VWLASDESRF VTASRVAVDL GSTQF
Validated NT Sequence
tcnttcgggc tttgttagca gccggatcct cgagaagctt ggctgcagaa cttgttcgtg ctgtttatta gaactgcgtc gaacccaggt cgacagccac ccggctcgcg gtgacgaagc gcgattcgtc gctcgccagc cacacggcgg cgtcggcgat gtcctcgggt tcggcgatgt agtccggcag gaacggcgtg accatctgca tcaggcccgg attggtgtcg ttggcgcgcg tgagggcggc cagcatgtcg ccggtaccca tcggggtgtt gacggcacct ggatgcaggc tgttgacccg gatccggtgc ttgcccagct cggcggcgaa cgcgcgggcc atgcctgcga cagcatgctt gctcgcggtg tagtgcacca tgaacggctg catcttgatg cccgccgcgg agctgatcag gatgatcgaa ccgccgcggc ccccgtcgat gatgtgttgg gcgccggcca tcacggtgtt ccaggtgccg gtgacgttga tgtccatgac gtcgcggaac gactcgggcg tggtctcgtt ccacggttcc gggacggtga tacccgcgtt ggcgacgatg acgtcgagcc tgccgaactc ggcgacaccc ttgccgacga cgtcgcgcag tgcctccagg tcgcgagtgt cggccctggc ggccaggatt cgccgacccg tggcctcgac caggcgggtg gtctcggcga gatcctccgg cgtcgccgaa tcgtagggca cgctgggtgg caaaggnccg gcgatgtcga ccgcgatgat gtcggcgccc tccttggcca tgcggaccgc atgcgcgcgg ccctgaccgc gggcggcccc ggtgatgaac gcgacccgtc cggtcagccg ctcggccatc gaaccnnncc ctgggtctga ncttcnngna cccatntgan gnnangtgat gancnngnnt atcncnntna gtnacaantn nntnntagnn ggnannnncn nnnnnnnnng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAERLTGRV AFITGAARGQ GRAHAVRMAK EGADIIAVDI AGPLPPSVPY DSATPEDLAE TTRLVEATGR RILAARADTR DLEALRDVVG KGVAEFGRLD VIVANAGITV PEPWNETTPE SFRDVMDINV TGTWNTVMAG AQHIIDGGRG GSIILISSAA GIKMQPFMVH YTASKHAVAG MARAFAAELG KHRIRVNSLH PGAVNTPMGT GDMLAALTRA NDTNPGLMQM VTPFLPDYIA EPEDIADAAV WLASDESRFV TASRVAVDLG STQF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCGAGCGG CTGACCGGAC GGGTCGCGTT CATCACCGGG GCCGCCCGCG GTCAGGGCCG CGCGCATGCG GTCCGCATGG CCAAGGAGGG CGCCGACATC ATCGCGGTCG ACATCGCCGG GCCTTTGCCA CCCAGCGTGC CCTACGATTC GGCGACGCCG GAGGATCTCG CCGAGACCAC CCGCCTGGTC GAGGCCACGG GTCGGCGAAT CCTGGCCGCC AGGGCCGACA CTCGCGACCT GGAGGCACTG CGCGACGTCG TCGGCAAGGG TGTCGCCGAG TTCGGCAGGC TCGACGTCAT CGTCGCCAAC GCGGGTATCA CCGTCCCGGA ACCGTGGAAC GAGACCACGC CCGAGTCGTT CCGCGACGTC ATGGACATCA ACGTCACCGG CACCTGGAAC ACCGTGATGG CCGGCGCCCA ACACATCATC GACGGGGGCC GCGGCGGTTC GATCATCCTG ATCAGCTCCG CGGCGGGCAT CAAGATGCAG CCGTTCATGG TGCACTACAC CGCGAGCAAG CATGCTGTCG CAGGCATGGC CCGCGCGTTC GCCGCCGAGC TGGGCAAGCA CCGGATCCGG GTCAACAGCC TGCATCCAGG TGCCGTCAAC ACCCCGATGG GTACCGGCGA CATGCTGGCC GCCCTCACGC GCGCCAACGA CACCAATCCG GGCCTGATGC AGATGGTCAC GCCGTTCCTG CCGGACTACA TCGCCGAACC CGAGGACATC GCCGACGCCG CCGTGTGGCT GGCGAGCGAC GAATCGCGCT TCGTCACCGC GAGCCGGGTG GCTGTCGACC TGGGTTCGAC GCAGTTCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA