MythA.01326.c
Carveol dehydrogenase (A0R518 homolog)
| CENTER ID: | MythA.01326.c |
| ORGANISM: | Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409 |
| ASSOCIATED DISEASE: | Respiratory Infection |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01326.c.A1.GE28783 | Full length( MythA.01326.c ) | 1 | 364 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MythA.01326.c.A1.PW28685 | Full length( MythA.01326.c ) | 1 | 364 |
Structures
3OEC
DEPOSITED: 8/12/2010
DETERMINATION: XRay
CLONE: MythA.01326.c.A1.GE28783
PROTEIN: MythA.01326.c.A1.PW28685
Publications by SSGCID
Mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable NAD cofactors
Abendroth J, Begley DW, Edwards TE, Gardberg AS, Lorimer DD, Myler PJ, Phan IQ, Pierce PG, Staker BL, Sullivan A, Marathias VM, Haft DH, Mayclin SJ
Sci Rep - 2016
volume 7, pages 41074
PMID: 28120876; PMCID: PMC5264612
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1078020.3.peg.3418 |
| RefSeq: | WP_003926949.1 |
| UniProt: | G7CJ87 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPVRRSTRV SARGQGARMN RLQGKVAFIT GAARGQGRTH AVRLAQDGAD IVAIDLCRQQ PNLDYAQGSP EELKETVRLV EEQGRRIIAR QADVRDLASL QAVVDEALAE FGHIDILVSN VGISNQGEVV SLTDQQWSDI LQTNLIGAWH ACRAVLPSMI ERGQGGSVIF VSSTVGLRGA PGQSHYAASK HGVQGLMLSL ANEVGRHNIR VNSVNPGAVN TEMALNEKLL KMFLPHLENP TREDAAELFS QLTLLPIPWV EPEDVSNAVA WLASDEARYX XXXXXXXXXX XSWPEHRRLE TPMKKISVDI EYCQAHGRCY ALFPDLFDAQ EDGTAVVKGD GELPDSIDPA DVTGSCPEAA ITVG
NT Sequence
GTGGATCCGG TACGACGAAG CACTCGAGTA TCCGCACGTG GCCAGGGAGC GCGCATGAAC CGATTGCAGG GCAAGGTCGC ATTCATCACC GGCGCCGCAC GCGGGCAGGG ACGGACCCAC GCGGTGCGAC TGGCCCAGGA CGGCGCCGAC ATCGTCGCCA TCGATCTCTG CCGCCAGCAG CCGAACCTCG ACTACGCCCA GGGGTCACCC GAGGAGCTGA AGGAGACGGT CCGGCTCGTC GAGGAGCAGG GCCGGCGGAT CATCGCCCGC CAGGCCGACG TCCGCGACCT GGCCTCGCTG CAGGCCGTGG TCGACGAGGC CCTGGCCGAA TTCGGCCACA TCGACATCCT GGTCTCCAAC GTCGGCATCT CGAACCAGGG TGAGGTCGTC AGCCTCACCG ATCAACAGTG GTCCGACATC CTGCAGACCA ACCTCATCGG TGCCTGGCAC GCCTGCCGCG CGGTGCTGCC GTCGATGATC GAACGCGGTC AGGGCGGCTC GGTGATCTTC GTGAGCTCCA CCGTCGGCCT ACGGGGAGCG CCCGGTCAGT CGCACTACGC GGCGTCCAAA CACGGTGTGC AGGGCCTGAT GCTGTCGCTG GCGAATGAAG TGGGGCGACA CAACATCCGG GTCAACTCGG TGAATCCGGG TGCGGTCAAC ACCGAGATGG CGCTCAACGA GAAGCTGCTC AAGATGTTCC TCCCGCACCT GGAGAACCCG ACGCGGGAAG ACGCCGCCGA ACTGTTCAGC CAGTTGACTC TGCTCCCCAT CCCGTGGGTC GAGCCCGAGG ACGTCAGCAA TGCCGTGGCC TGGCTGGCCT CGGACGAGGC CCGCTACATN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTCAGTTGGC CCGAGCATAG GAGGTTGGAG ACACCGATGA AGAAAATTTC CGTCGATATC GAGTACTGCC AGGCGCACGG CCGCTGCTAT GCGCTGTTCC CCGATCTGTT CGACGCCCAG GAGGACGGCA CCGCCGTCGT CAAAGGCGAT GGGGAACTGC CGGATTCGAT CGACCCGGCA GACGTGACGG GATCCTGTCC CGAGGCCGCG ATCACCGTTG GT
Details for MythA.01326.c.A1.GE28783
| HARVESTED ON: | 3/1/2010 |
| SEQUENCED ON: | 5/19/2010 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 82 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPVRRST RVSARGQGAR MNRLQGKVAF ITGAARGQGR THAVRLAQDG ADIVAIDLCR QQPNLDYAQG SPEELKETVR LVEEQGRRII ARQADVRDLA SLQAVVDEAL AEFGHIDILV SNVGISNQGE VVSLTDQQWS DILQTNLIGA WHACRAVLPS MIERGQGGSV IFVSSTVGLR GAPGQSHYAA SKHGVQGLML SLANEVGRHN IRVNSVNPGA VNTEMALNEK LLKMFLPHLE NPTREDAAEL FSQLTLLPIP WVEPEDVSNA VAWLASDEAR YIHGAAIPVD GGQLARA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg atccggtacg acgaagcact cgagtatccg cacgtggcca gggagcgcgc atgaaccgat tgcagggcaa ggtcgcattc atcaccggcg ccgcacgcgg gcagggacgg acccacgcgg tgcgactggc ccaggacggc gccgacatcg tcgccatcga tctctgccgc cagcagccga acctcgacta cgcccagggg tcacccgagg agctgaagga gacggtccgg ctcgtcgagg agcagggccg gcggatcatc gcccgccagg ccgacgtccg cgacctggcc tcgctgcagg ccgtggtcga cgaggccctg gccgaattcg gccacatcga catcctggtc tccaacgtcg gcatctcgaa ccagggtgag gtcgtcagcc tcaccgatca acagtggtcc gacatcctgc agaccaacct catcggtgcc tggcacgcct gccgcgcggt gctgccgtcg atgatcgaac gcggtcaggg cggctcggtg atcttcgtga gctccaccgt cggcctacgg ggagcgcccg gtcagtcgca ctacgcggcg tccaaacacg gtgtgcaggg cctgatgctg tcgctggcga atgaagtggg gcgacacaac atccgggtca actcggtgaa tccgggtgcg gtcaacaccg agatggcgct caacgagaag ctgctcaaga tgttcctccc gcacctggag aacccgacgc gggaagacgc cgccgaactg ttcagccagt tgactctgct ccccatcccg tgggtcgagc ccgaggacgt cagcaatgcc gtggcctggc tggcctcgga cgaggcccgc tacatccacg gggccgccat ccccgtcgac ggcggtcagt tggcccgagc ataggaggtt ggagacaccg atgaagaaaa tttccgtcga tatcgagtac tgccaggcgc acggccgctg ctatgcgctg ttccccgatc tgttcgacgc ccaggaggac ggcaccgccg tcgtcaaagg cgatggggaa ctgccggatt cgatcgaccc ggcagacgtg acgggatcct gtcccgaggc cgcgatcacc gttggttaat aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPVRRST RVSARGQGAR MNRLQGKVAF ITGAARGQGR THAVRLAQDG ADIVAIDLCR QQPNLDYAQG SPEELKETVR LVEEQGRRII ARQADVRDLA SLQAVVDEAL AEFGHIDILV SNVGISNQGE VVSLTDQQWS DILQTNLIGA WHACRAVLPS MIERGQGGSV IFVSSTVGLR GAPGQSHYAA SKHGVQGLML SLANEVGRHN IRVNSVNPGA VNTEMALNEK LLKMFLPHLE NPTREDAAEL FSQLTLLPIP WVEPEDVSNA VAWLASDEAR YXXXXXXXXX XXXSWPEHRR LETPMKKISV DIEYCQAHGR CYALFPDLFD AQEDGTAVVK GDGELPDSID PADVTGSCPE AAITVG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggatccg gtacgacgaa gcactcgagt atccgcacgt ggccagggag cgcgcatgaa ccgattgcag ggcaaggtcg cattcatcac cggcgccgca cgcgggcagg gacggaccca cgcggtgcga ctggcccagg acggcgccga catcgtcgcc atcgatctct gccgccagca gccgaacctc gactacgccc aggggtcacc cgaggagctg aaggagacgg tccggctcgt cgaggagcag ggccggcgga tcatcgcccg ccaggccgac gtccgcgacc tggcctcgct gcaggccgtg gtcgacgagg ccctggccga attcggccac atcgacatcc tggtctccaa cgtcggcatc tcgaaccagg gtgaggtcgt cagcctcacc gatcaacagt ggtccgacat cctgcagacc aacctcatcg gtgcctggca cgcctgccgc gcggtgctgc cgtcgatgat cgaacgcggt cagggcggct cggtgatctt cgtgagctcc accgtcggcc tacggggagc gcccggtcag tcgcactacg cggcgtccaa acacggtgtg cagggcctga tgctgtcgct ggcgaatgaa gtggggcgac acaacatccg ggtcaactcg gtgaatccgg gtgcggtcaa caccgagatg gcgctcaacg agaagctgct caagatgttc ctcccgcacc tggagaaccc gacgcgggaa gacgccgccg aactgttcag ccagttgact ctgctcccca tcccgtgggt cgagcccgag gacgtcagca atgccgtggc ctggctggcc tcggacgagg cccgctacat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcagttgg cccgagcata ggaggttgga gacaccgatg aagaaaattt ccgtcgatat cgagtactgc caggcgcacg gccgctgcta tgcgctgttc cccgatctgt tcgacgccca ggaggacggc accgccgtcg tcaaaggcga tggggaactg ccggattcga tcgacccggc agacgtgacg ggatcctgtc ccgaggccgc gatcaccgtt ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MythA.01326.c.A1.PW28685
| PURIFICATION DATe: | 6/14/2010 |
| CONCENTRATION: | 29.46mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 96 |
| PERCENT COVERAGE: | 82 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPVRRST RVSARGQGAR MNRLQGKVAF ITGAARGQGR THAVRLAQDG ADIVAIDLCR QQPNLDYAQG SPEELKETVR LVEEQGRRII ARQADVRDLA SLQAVVDEAL AEFGHIDILV SNVGISNQGE VVSLTDQQWS DILQTNLIGA WHACRAVLPS MIERGQGGSV IFVSSTVGLR GAPGQSHYAA SKHGVQGLML SLANEVGRHN IRVNSVNPGA VNTEMALNEK LLKMFLPHLE NPTREDAAEL FSQLTLLPIP WVEPEDVSNA VAWLASDEAR YIHGAAIPVD GGQLARA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg atccggtacg acgaagcact cgagtatccg cacgtggcca gggagcgcgc atgaaccgat tgcagggcaa ggtcgcattc atcaccggcg ccgcacgcgg gcagggacgg acccacgcgg tgcgactggc ccaggacggc gccgacatcg tcgccatcga tctctgccgc cagcagccga acctcgacta cgcccagggg tcacccgagg agctgaagga gacggtccgg ctcgtcgagg agcagggccg gcggatcatc gcccgccagg ccgacgtccg cgacctggcc tcgctgcagg ccgtggtcga cgaggccctg gccgaattcg gccacatcga catcctggtc tccaacgtcg gcatctcgaa ccagggtgag gtcgtcagcc tcaccgatca acagtggtcc gacatcctgc agaccaacct catcggtgcc tggcacgcct gccgcgcggt gctgccgtcg atgatcgaac gcggtcaggg cggctcggtg atcttcgtga gctccaccgt cggcctacgg ggagcgcccg gtcagtcgca ctacgcggcg tccaaacacg gtgtgcaggg cctgatgctg tcgctggcga atgaagtggg gcgacacaac atccgggtca actcggtgaa tccgggtgcg gtcaacaccg agatggcgct caacgagaag ctgctcaaga tgttcctccc gcacctggag aacccgacgc gggaagacgc cgccgaactg ttcagccagt tgactctgct ccccatcccg tgggtcgagc ccgaggacgt cagcaatgcc gtggcctggc tggcctcgga cgaggcccgc tacatccacg gggccgccat ccccgtcgac ggcggtcagt tggcccgagc ataggaggtt ggagacaccg atgaagaaaa tttccgtcga tatcgagtac tgccaggcgc acggccgctg ctatgcgctg ttccccgatc tgttcgacgc ccaggaggac ggcaccgccg tcgtcaaagg cgatggggaa ctgccggatt cgatcgaccc ggcagacgtg acgggatcct gtcccgaggc cgcgatcacc gttggttaat aa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVDPVRRST RVSARGQGAR MNRLQGKVAF ITGAARGQGR THAVRLAQDG ADIVAIDLCR QQPNLDYAQG SPEELKETVR LVEEQGRRII ARQADVRDLA SLQAVVDEAL AEFGHIDILV SNVGISNQGE VVSLTDQQWS DILQTNLIGA WHACRAVLPS MIERGQGGSV IFVSSTVGLR GAPGQSHYAA SKHGVQGLML SLANEVGRHN IRVNSVNPGA VNTEMALNEK LLKMFLPHLE NPTREDAAEL FSQLTLLPIP WVEPEDVSNA VAWLASDEAR YXXXXXXXXX XXXSWPEHRR LETPMKKISV DIEYCQAHGR CYALFPDLFD AQEDGTAVVK GDGELPDSID PADVTGSCPE AAITVG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGATCCG GTACGACGAA GCACTCGAGT ATCCGCACGT GGCCAGGGAG CGCGCATGAA CCGATTGCAG GGCAAGGTCG CATTCATCAC CGGCGCCGCA CGCGGGCAGG GACGGACCCA CGCGGTGCGA CTGGCCCAGG ACGGCGCCGA CATCGTCGCC ATCGATCTCT GCCGCCAGCA GCCGAACCTC GACTACGCCC AGGGGTCACC CGAGGAGCTG AAGGAGACGG TCCGGCTCGT CGAGGAGCAG GGCCGGCGGA TCATCGCCCG CCAGGCCGAC GTCCGCGACC TGGCCTCGCT GCAGGCCGTG GTCGACGAGG CCCTGGCCGA ATTCGGCCAC ATCGACATCC TGGTCTCCAA CGTCGGCATC TCGAACCAGG GTGAGGTCGT CAGCCTCACC GATCAACAGT GGTCCGACAT CCTGCAGACC AACCTCATCG GTGCCTGGCA CGCCTGCCGC GCGGTGCTGC CGTCGATGAT CGAACGCGGT CAGGGCGGCT CGGTGATCTT CGTGAGCTCC ACCGTCGGCC TACGGGGAGC GCCCGGTCAG TCGCACTACG CGGCGTCCAA ACACGGTGTG CAGGGCCTGA TGCTGTCGCT GGCGAATGAA GTGGGGCGAC ACAACATCCG GGTCAACTCG GTGAATCCGG GTGCGGTCAA CACCGAGATG GCGCTCAACG AGAAGCTGCT CAAGATGTTC CTCCCGCACC TGGAGAACCC GACGCGGGAA GACGCCGCCG AACTGTTCAG CCAGTTGACT CTGCTCCCCA TCCCGTGGGT CGAGCCCGAG GACGTCAGCA ATGCCGTGGC CTGGCTGGCC TCGGACGAGG CCCGCTACAT NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTCAGTTGG CCCGAGCATA GGAGGTTGGA GACACCGATG AAGAAAATTT CCGTCGATAT CGAGTACTGC CAGGCGCACG GCCGCTGCTA TGCGCTGTTC CCCGATCTGT TCGACGCCCA GGAGGACGGC ACCGCCGTCG TCAAAGGCGA TGGGGAACTG CCGGATTCGA TCGACCCGGC AGACGTGACG GGATCCTGTC CCGAGGCCGC GATCACCGTT GGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA