MypaA.01326.k

Putative uncharacterized protein

CENTER ID: MypaA.01326.k
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01326.k.A1.GE29582 Full length( MypaA.01326.k ) 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.01326.k.A1.PW29480 Full length( MypaA.01326.k ) 1 270

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|262316.17.peg.1453
RefSeq: NP_960314.1
UniProt: Q740H1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADNKVALIT GAARGQGRAH AVRLSAGGAD IIAVDIAGRL PESVPYESPT RDDLAETARL VEANGRRAIT AAIDVRDAEK LSAAVGHAVA ELGRLDIIVA NAGICCPAPW DQITGQAFRD TIDTNVIGTW NTVMAGAHHI IAGRRGGSII LIGSAAGVKM QAFMVHYTAS KHAITGMARA FAAELGRYNI RVNSLHPGAV DTPMGTGRMR DALESAAATY PHLEGLHKPL LPDGIAQPED IADAVAWLAS DQSRFVTASG ISVDLGVAYC
NT Sequence
TTGGCGGACA ACAAAGTAGC TCTCATCACC GGAGCCGCGC GCGGGCAGGG ACGTGCCCAC GCGGTGCGGC TGAGCGCCGG CGGCGCCGAC ATAATCGCGG TCGACATCGC CGGGCGGCTA CCGGAAAGCG TCCCATATGA GTCGCCGACT CGCGATGATC TCGCCGAGAC CGCGCGGCTC GTTGAAGCCA ACGGCCGGCG CGCGATCACC GCGGCGATCG ATGTGCGCGA CGCCGAAAAG CTGAGCGCGG CCGTCGGCCA CGCGGTCGCC GAGCTCGGCC GCCTCGACAT CATCGTCGCC AACGCGGGAA TTTGTTGCCC GGCACCATGG GACCAAATCA CGGGCCAGGC GTTCCGCGAC ACCATCGACA CCAACGTGAT CGGCACGTGG AACACCGTCA TGGCAGGCGC TCACCACATC ATCGCAGGGC GACGGGGCGG GTCGATCATC CTGATCGGTT CCGCTGCAGG TGTGAAAATG CAAGCGTTCA TGGTGCATTA CACGGCGAGC AAGCACGCCA TCACCGGCAT GGCCCGGGCC TTCGCCGCCG AGCTGGGCCG CTACAACATC CGCGTTAACA GCCTTCACCC CGGAGCTGTC GACACACCCA TGGGAACCGG ACGTATGCGC GACGCGCTCG AGTCGGCGGC CGCGACGTAT CCCCATTTGG AAGGCCTGCA CAAGCCGCTG CTGCCCGACG GCATTGCGCA ACCCGAAGAC ATCGCCGACG CGGTGGCCTG GCTCGCATCG GATCAATCGA GATTCGTTAC CGCATCCGGT ATCTCGGTCG ACCTGGGTGT CGCCTACTGC
Details for MypaA.01326.k.A1.GE29582
HARVESTED ON: 6/15/2010
SEQUENCED ON: 6/27/2010
EXPECTED MW: 30kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLADNKVAL ITGAARGQGR AHAVRLSAGG ADIIAVDIAG RLPESVPYES PTRDDLAETA RLVEANGRRA ITAAIDVRDA EKLSAAVGHA VAELGRLDII VANAGICCPA PWDQITGQAF RDTIDTNVIG TWNTVMAGAH HIIAGRRGGS IILIGSAAGV KMQAFMVHYT ASKHAITGMA RAFAAELGRY NIRVNSLHPG AVDTPMGTGR MRDALESAAA TYPHLEGLHK PLLPDGIAQP EDIADAVAWL ASDQSRFVTA SGISVDLGVA
Validated NT Sequence
aggcgacacc caggtcgacc gagataccgg atgcggtaac gaatctcgat tgatccgatg cgagccaggc caccgcgtcg gcgatgtctt cgggttgcgc aatgccgtcg ggcagcagcg gcttgtgcag gccttccaaa tggggatacg tcgcggccgc cgactcgagc gcgtcgcgca tacgtccggt tcccatgggt gtgtcgacag ctccggggtg aaggctgtta acgcggatgt tgtagcggcc cagctcggcg gcgaaggccc gggccatgcc ggtgatggcg tgcttgctcg ccgtgtaatg caccatgaac gcttgcattt tcacacctgc agcggaaccg atcaggatga tcgacccgcc ccgtcgccct gcgatgatgt ggtgagcgcc tgccatgacg gtgttccacg tgccgatcac gttggtgtcg atggtgtcgc ggaacgcctg gcccgtgatt tggtcccatg gtgccgggca acaaattccc gcgttggcga cgatgatgtc gaggcggccg agctcggcga ccgcgtggcc gacggccgcg ctcagctttt cggcgtcgcg cacatcgatc gccgcggtga tcgcgcgccg gccgttggct tcaacgagcc gcgcggtctc ggcgagatca tcgcgagtcg gcgactcata tgggacgctt tccggtagcc gcccggcgat gtcgaccgcg attatgtcgg cgccgccggc gctcagccgc accgcgtggg cacgtccctg cccgcgcgcg gctccggtga tgagagctac tttgttgtcc gccaacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLADNKVAL ITGAARGQGR AHAVRLSAGG ADIIAVDIAG RLPESVPYES PTRDDLAETA RLVEANGRRA ITAAIDVRDA EKLSAAVGHA VAELGRLDII VANAGICCPA PWDQITGQAF RDTIDTNVIG TWNTVMAGAH HIIAGRRGGS IILIGSAAGV KMQAFMVHYT ASKHAITGMA RAFAAELGRY NIRVNSLHPG AVDTPMGTGR MRDALESAAA TYPHLEGLHK PLLPDGIAQP EDIADAVAWL ASDQSRFVTA SGISVDLGVA YC
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggcggac aacaaagtag ctctcatcac cggagccgcg cgcgggcagg gacgtgccca cgcggtgcgg ctgagcgccg gcggcgccga cataatcgcg gtcgacatcg ccgggcggct accggaaagc gtcccatatg agtcgccgac tcgcgatgat ctcgccgaga ccgcgcggct cgttgaagcc aacggccggc gcgcgatcac cgcggcgatc gatgtgcgcg acgccgaaaa gctgagcgcg gccgtcggcc acgcggtcgc cgagctcggc cgcctcgaca tcatcgtcgc caacgcggga atttgttgcc cggcaccatg ggaccaaatc acgggccagg cgttccgcga caccatcgac accaacgtga tcggcacgtg gaacaccgtc atggcaggcg ctcaccacat catcgcaggg cgacggggcg ggtcgatcat cctgatcggt tccgctgcag gtgtgaaaat gcaagcgttc atggtgcatt acacggcgag caagcacgcc atcaccggca tggcccgggc cttcgccgcc gagctgggcc gctacaacat ccgcgttaac agccttcacc ccggagctgt cgacacaccc atgggaaccg gacgtatgcg cgacgcgctc gagtcggcgg ccgcgacgta tccccatttg gaaggcctgc acaagccgct gctgcccgac ggcattgcgc aacccgaaga catcgccgac gcggtggcct ggctcgcatc ggatcaatcg agattcgtta ccgcatccgg tatctcggtc gacctgggtg tcgcctactg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MypaA.01326.k.A1.PW29480
PURIFICATION DATe: 8/30/2010
CONCENTRATION: 25.94mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLADNKVAL ITGAARGQGR AHAVRLSAGG ADIIAVDIAG RLPESVPYES PTRDDLAETA RLVEANGRRA ITAAIDVRDA EKLSAAVGHA VAELGRLDII VANAGICCPA PWDQITGQAF RDTIDTNVIG TWNTVMAGAH HIIAGRRGGS IILIGSAAGV KMQAFMVHYT ASKHAITGMA RAFAAELGRY NIRVNSLHPG AVDTPMGTGR MRDALESAAA TYPHLEGLHK PLLPDGIAQP EDIADAVAWL ASDQSRFVTA SGISVDLGVA
Validated NT Sequence
aggcgacacc caggtcgacc gagataccgg atgcggtaac gaatctcgat tgatccgatg cgagccaggc caccgcgtcg gcgatgtctt cgggttgcgc aatgccgtcg ggcagcagcg gcttgtgcag gccttccaaa tggggatacg tcgcggccgc cgactcgagc gcgtcgcgca tacgtccggt tcccatgggt gtgtcgacag ctccggggtg aaggctgtta acgcggatgt tgtagcggcc cagctcggcg gcgaaggccc gggccatgcc ggtgatggcg tgcttgctcg ccgtgtaatg caccatgaac gcttgcattt tcacacctgc agcggaaccg atcaggatga tcgacccgcc ccgtcgccct gcgatgatgt ggtgagcgcc tgccatgacg gtgttccacg tgccgatcac gttggtgtcg atggtgtcgc ggaacgcctg gcccgtgatt tggtcccatg gtgccgggca acaaattccc gcgttggcga cgatgatgtc gaggcggccg agctcggcga ccgcgtggcc gacggccgcg ctcagctttt cggcgtcgcg cacatcgatc gccgcggtga tcgcgcgccg gccgttggct tcaacgagcc gcgcggtctc ggcgagatca tcgcgagtcg gcgactcata tgggacgctt tccggtagcc gcccggcgat gtcgaccgcg attatgtcgg cgccgccggc gctcagccgc accgcgtggg cacgtccctg cccgcgcgcg gctccggtga tgagagctac tttgttgtcc gccaacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLADNKVAL ITGAARGQGR AHAVRLSAGG ADIIAVDIAG RLPESVPYES PTRDDLAETA RLVEANGRRA ITAAIDVRDA EKLSAAVGHA VAELGRLDII VANAGICCPA PWDQITGQAF RDTIDTNVIG TWNTVMAGAH HIIAGRRGGS IILIGSAAGV KMQAFMVHYT ASKHAITGMA RAFAAELGRY NIRVNSLHPG AVDTPMGTGR MRDALESAAA TYPHLEGLHK PLLPDGIAQP EDIADAVAWL ASDQSRFVTA SGISVDLGVA YC
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTTGGCGGAC AACAAAGTAG CTCTCATCAC CGGAGCCGCG CGCGGGCAGG GACGTGCCCA CGCGGTGCGG CTGAGCGCCG GCGGCGCCGA CATAATCGCG GTCGACATCG CCGGGCGGCT ACCGGAAAGC GTCCCATATG AGTCGCCGAC TCGCGATGAT CTCGCCGAGA CCGCGCGGCT CGTTGAAGCC AACGGCCGGC GCGCGATCAC CGCGGCGATC GATGTGCGCG ACGCCGAAAA GCTGAGCGCG GCCGTCGGCC ACGCGGTCGC CGAGCTCGGC CGCCTCGACA TCATCGTCGC CAACGCGGGA ATTTGTTGCC CGGCACCATG GGACCAAATC ACGGGCCAGG CGTTCCGCGA CACCATCGAC ACCAACGTGA TCGGCACGTG GAACACCGTC ATGGCAGGCG CTCACCACAT CATCGCAGGG CGACGGGGCG GGTCGATCAT CCTGATCGGT TCCGCTGCAG GTGTGAAAAT GCAAGCGTTC ATGGTGCATT ACACGGCGAG CAAGCACGCC ATCACCGGCA TGGCCCGGGC CTTCGCCGCC GAGCTGGGCC GCTACAACAT CCGCGTTAAC AGCCTTCACC CCGGAGCTGT CGACACACCC ATGGGAACCG GACGTATGCG CGACGCGCTC GAGTCGGCGG CCGCGACGTA TCCCCATTTG GAAGGCCTGC ACAAGCCGCT GCTGCCCGAC GGCATTGCGC AACCCGAAGA CATCGCCGAC GCGGTGGCCT GGCTCGCATC GGATCAATCG AGATTCGTTA CCGCATCCGG TATCTCGGTC GACCTGGGTG TCGCCTACTG CAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA