MytuD.10611.b

POSSIBLE ZINC-CONTAINING ALCOHOL DEHYDROGENASE NAD DEPENDENT ADHB

CENTER ID: MytuD.10611.b
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.10611.b.A1.GE27896 Full length( MytuD.10611.b ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127257
PATRIC ID: fig|83332.12.peg.851
RefSeq: YP_177754.1
TubercuList: Rv0761c
MTB Network Portal: Rv0761c
UniProt: P9WQC7
Orthologues for Rv0761c
Species % Identity % Coverage Clones Proteins Structures
avium 87 100 1 0 0
marinum 87 100 1 0 0
ulcerans 87 100 1 0 0
thermoresistibile 84 100 2 1 0
smegmatis 81 100 0 0 0
abscessus 59 100 1 0 0
smegmatis 58 100 0 0 0
thermoresistibile 57 100 1 0 0
marinum 51 99 1 0 0
paratuberculosis 46 99 1 1 0
avium 45 99 1 0 0
avium 45 97 1 0 0
marinum 44 99 1 0 0
ulcerans 43 99 0 0 0
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTKGALIWE FNQPWSVEEI EIGDPRKDEV KIQMEAAGMC RSDHHLVTGD IPMAGFPVLG GHEGAGIVTE VGPGVDDFAP GDHVVLAFIP SCGKCPSCQA GMRNLCDLGA GLLAGESVTD GSFRIQARGQ NVYPMTLLGT FSPYMVVHRS SVVKIDPSVP FEVACLVGCG VTTGYGSAVR TADVRPGDDV AIVGLGGVGM AALQGAVSAG ARYVFAVEPV EWKRDQALKF GATHVYPDIN AALMGIAEVT YGLMAQKVII TVGKLDGADV DSYLTITAKG GTCVLTAIGS LVDTQVTLNL AMLTLLQKNI QGTIFGGGNP HYDIPKLLSM YKAGKLNLDD MVTTAYKLEQ INDGYQDMLN GKNIRGVIRY TDDDR
NT Sequence
GTGAAGACCA AGGGCGCACT GATCTGGGAG TTCAACCAGC CATGGTCCGT CGAAGAGATC GAAATCGGCG ACCCGCGCAA GGACGAAGTC AAGATCCAGA TGGAAGCGGC TGGGATGTGC CGCTCCGACC ATCACCTGGT GACGGGCGAC ATCCCGATGG CGGGCTTTCC CGTTCTGGGC GGACACGAGG GCGCGGGCAT CGTCACCGAG GTCGGCCCGG GAGTCGACGA CTTCGCCCCG GGCGATCACG TGGTGTTGGC ATTCATCCCG TCCTGCGGCA AGTGTCCGTC CTGCCAGGCT GGAATGCGGA ATCTGTGCGA CCTGGGGGCG GGGCTGCTCG CCGGGGAATC TGTGACGGAC GGCTCCTTCC GGATTCAGGC TCGCGGCCAG AACGTCTACC CGATGACCCT GCTCGGAACG TTTTCACCGT ACATGGTGGT GCACCGCAGC TCGGTGGTGA AGATCGACCC GTCGGTGCCC TTCGAAGTCG CCTGCCTGGT TGGTTGCGGC GTCACCACCG GCTATGGTTC GGCGGTCCGC ACGGCCGACG TCCGGCCGGG CGACGACGTG GCCATCGTCG GCTTGGGTGG GGTCGGCATG GCGGCGTTGC AGGGCGCGGT CAGCGCGGGC GCCCGCTACG TCTTCGCGGT GGAGCCGGTG GAATGGAAAC GTGATCAGGC TCTGAAATTC GGTGCCACCC ACGTCTACCC GGACATCAAC GCCGCGCTGA TGGGCATTGC CGAGGTCACC TACGGCCTGA TGGCGCAGAA GGTGATCATC ACCGTCGGCA AGCTCGATGG CGCCGACGTC GACAGCTATC TGACCATCAC GGCCAAGGGC GGCACCTGCG TGCTGACGGC CATCGGCAGC CTGGTCGACA CCCAGGTGAC GCTGAACCTC GCGATGTTGA CCCTGCTGCA AAAGAACATC CAGGGCACCA TCTTCGGCGG CGGCAACCCG CACTACGACA TTCCGAAGCT GTTGTCGATG TATAAGGCCG GCAAACTCAA CCTCGACGAC ATGGTGACCA CTGCGTACAA GCTGGAGCAG ATCAACGACG GATACCAGGA CATGCTGAAC GGCAAGAACA TTCGCGGCGT GATCCGGTAC ACGGACGACG ACCGC
Details for MytuD.10611.b.A1.GE27896
HARVESTED ON: 11/3/2009
SEQUENCED ON: 11/10/2009
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKTKGALI WEFNQPWSVE EIEIGDPRKD EVKIQMEAAG MCRSDHHLVT GDIPMAGFPV LGGHEGAGIV TEVGPGVDDF APGDHVVLAF IPSCGKCPSC QAGMRNLCDL GAGLLAGESV TDGSFRIQAR GQNVYPMTLL GTFSPYMVVH RSSVVKIDPS VPFEVACLVG CGVTTGYGSA VRTADVRPGD DVAIVGLGGV GMAALQGAVS AGARYVFAVE PVEWKRDQAL KFGATHVYPD INAALMGIAE VTYGLMAQKV IITVGKLDGA DVDSYLTITA KGGTCVLTAI GSLVDTQVTL NLAMLTLLQK NIQGTIFGGG NPHYDIPKLL SMYKAGKLNL DDMVTTAYKL EQINDGYQDM LNGKNIRGVI RYTDD
Validated NT Sequence
cgtcgtccgt gtaccggatc acgccgcgaa tgttcttgcc gttcagcatg tcctggtatc cgtcgttgat ctgctccagc ttgtacgcag tggtcaccat gtcgtcgagg ttgagtttgc cggccttata catcgacaac agcttcggaa tgtcgtagtg cgggttgccg ccgccgaaga tggtgccctg gatgttcttt tgcagcaggg tcaacatcgc gaggttcagc gtcacctggg tgtcgaccag gctgccgatg gccgtcagca cgcaggtgcc gcccttggcc gtgatggtca gatagctgtc gacgtcggcg ccatcgagct tgccgacggt gatgatcacc ttctgcgcca tcaggccgta ggtgacctcg gcaatgccca tcagcgcggc gttgatgtcc gggtagacgt gggtggcacc gaatttcaga gcctgatcac gtttccattc caccggctcc accgcgaaga cgtagcgggc gcccgcgctg accgcgccct gcaacgccgc catgccgacc ccacccaagc cgacgatggc cacgtcgtcg cccggccgga cgtcggccgt gcggaccgcc gaaccatagc cggtggtgac gccgcaacca accaggcagg cgacttcgaa gggcaccgac gggtcgatct tcaccaccga gctgcggtgc accaccatgt acggtgaaaa cgttccgagc agggtcatcg ggtagacgtt ctggccgcga gcctgaatcc ggaaggagcc gtccgtcaca gattccccgg cgagcagccc cgcccccagg tcgcacagat tccgcattcc agcctggcag gacggacact tgccgcagga cgggatgaat gccaacacca cgtgatcgcc cggggcgaag tcgtcgactc ccgggccgac ctcggtgacg atgcccgcgc cctcgtgtcc gcccagaacg ggaaagcccg ccatcgggat gtcgcccgtc accaggtgat ggtcggagcg gcacatccca gccgcttcca tctggatctt gacttcgtcc ttgcgcgggt cgccgatttc gatctcttcg acggaccatg gctggttgaa ctcccagatc agtgcgccct tggtcttcac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta tntctcct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKTKGALI WEFNQPWSVE EIEIGDPRKD EVKIQMEAAG MCRSDHHLVT GDIPMAGFPV LGGHEGAGIV TEVGPGVDDF APGDHVVLAF IPSCGKCPSC QAGMRNLCDL GAGLLAGESV TDGSFRIQAR GQNVYPMTLL GTFSPYMVVH RSSVVKIDPS VPFEVACLVG CGVTTGYGSA VRTADVRPGD DVAIVGLGGV GMAALQGAVS AGARYVFAVE PVEWKRDQAL KFGATHVYPD INAALMGIAE VTYGLMAQKV IITVGKLDGA DVDSYLTITA KGGTCVLTAI GSLVDTQVTL NLAMLTLLQK NIQGTIFGGG NPHYDIPKLL SMYKAGKLNL DDMVTTAYKL EQINDGYQDM LNGKNIRGVI RYTDDDR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagacc aagggcgcac tgatctggga gttcaaccag ccatggtccg tcgaagagat cgaaatcggc gacccgcgca aggacgaagt caagatccag atggaagcgg ctgggatgtg ccgctccgac catcacctgg tgacgggcga catcccgatg gcgggctttc ccgttctggg cggacacgag ggcgcgggca tcgtcaccga ggtcggcccg ggagtcgacg acttcgcccc gggcgatcac gtggtgttgg cattcatccc gtcctgcggc aagtgtccgt cctgccaggc tggaatgcgg aatctgtgcg acctgggggc ggggctgctc gccggggaat ctgtgacgga cggctccttc cggattcagg ctcgcggcca gaacgtctac ccgatgaccc tgctcggaac gttttcaccg tacatggtgg tgcaccgcag ctcggtggtg aagatcgacc cgtcggtgcc cttcgaagtc gcctgcctgg ttggttgcgg cgtcaccacc ggctatggtt cggcggtccg cacggccgac gtccggccgg gcgacgacgt ggccatcgtc ggcttgggtg gggtcggcat ggcggcgttg cagggcgcgg tcagcgcggg cgcccgctac gtcttcgcgg tggagccggt ggaatggaaa cgtgatcagg ctctgaaatt cggtgccacc cacgtctacc cggacatcaa cgccgcgctg atgggcattg ccgaggtcac ctacggcctg atggcgcaga aggtgatcat caccgtcggc aagctcgatg gcgccgacgt cgacagctat ctgaccatca cggccaaggg cggcacctgc gtgctgacgg ccatcggcag cctggtcgac acccaggtga cgctgaacct cgcgatgttg accctgctgc aaaagaacat ccagggcacc atcttcggcg gcggcaaccc gcactacgac attccgaagc tgttgtcgat gtataaggcc ggcaaactca acctcgacga catggtgacc actgcgtaca agctggagca gatcaacgac ggataccagg acatgctgaa cggcaagaac attcgcggcg tgatccggta cacggacgac gaccgcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa