MyavA.10611.a

Alcohol dehydrogenase B

CENTER ID:	MyavA.10611.a
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.10611.a.A1.GE29167	Full length( MyavA.10611.a )		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127257
PATRIC ID:	fig\|243243.7.peg.684
RefSeq:	YP_879981.1
UniProt:	A0A0H2ZXU2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTKGALIWE FNQPWSIEEI EIGDPQAHEV KIQMEAAGMC HSDHHLVTGG IPMAGFPVLG GHEGAGIVTE VGPGVEDIAP GDHVVLSFIP SCGSCPTCQA GMRNLCDLGA GLLGGEAVSD GTFRIQARGQ NVYPMTLLGT FSPYMVVHRS SVVKIDPSIP FEVAALVGCG VTTGYGSAVR TADIRPGQDV AIVGVGGVGM AALQGAVNAG ARYIFAIDPV EWKRDQALKF GATHVYPDIM AAMAGMAEVT YGLMAHKVVV TVGELQGADV DNYLNITQKG GTCVLTAIGS LLDTNVTLNL AMLTLMQKNL QGTIFGGGNP QYDIPQLLSM YKAGKLNLDD MITRQYRLEQ INDGYQDMLD GKNIRGVIRY TDADR

NT Sequence

GTGAAGACAA AAGGCGCACT GATCTGGGAG TTCAACCAGC CCTGGTCCAT CGAGGAAATC GAGATCGGCG ACCCGCAAGC GCATGAGGTC AAGATCCAGA TGGAAGCGGC GGGCATGTGC CACTCCGACC ATCACCTCGT CACCGGCGGC ATCCCGATGG CCGGCTTCCC GGTGCTCGGC GGGCACGAGG GCGCGGGCAT CGTCACCGAG GTCGGCCCCG GCGTCGAGGA CATCGCCCCG GGCGACCACG TGGTGCTGTC GTTCATCCCG TCCTGCGGCT CGTGTCCGAC CTGCCAGGCG GGCATGCGCA ACCTGTGTGA CCTGGGTGCG GGTCTGCTGG GCGGCGAAGC GGTGTCCGAC GGGACCTTCC GCATCCAGGC CCGCGGCCAG AACGTCTACC CCATGACGCT GCTGGGCACG TTTTCTCCGT ACATGGTGGT GCACCGCAGC TCGGTGGTGA AGATCGACCC GTCGATCCCG TTCGAGGTGG CCGCGCTGGT CGGCTGCGGC GTCACCACCG GTTACGGGTC GGCGGTCCGC ACCGCCGACA TCCGGCCGGG CCAGGACGTC GCCATCGTCG GCGTCGGCGG CGTGGGCATG GCGGCGCTGC AGGGCGCGGT CAACGCCGGC GCCCGCTACA TCTTCGCGAT CGACCCGGTG GAGTGGAAGC GCGACCAGGC ACTGAAGTTC GGCGCCACCC ACGTCTACCC CGACATCATG GCCGCCATGG CCGGCATGGC CGAGGTGACC TACGGCCTGA TGGCCCACAA GGTCGTCGTC ACCGTGGGCG AGCTGCAGGG CGCCGACGTC GACAACTACC TGAACATCAC CCAGAAGGGC GGCACCTGCG TGCTGACCGC CATCGGCAGC CTGCTGGACA CCAACGTGAC GCTGAACCTG GCGATGCTGA CCCTGATGCA GAAGAATTTG CAGGGCACCA TCTTTGGTGG CGGCAACCCG CAGTACGACA TCCCGCAGCT GCTGTCGATG TACAAGGCCG GCAAGCTCAA CCTGGACGAC ATGATCACCC GCCAGTACCG GCTCGAGCAG ATCAACGACG GCTATCAGGA CATGTTGGAC GGCAAGAACA TTCGCGGCGT CATCCGCTAC ACCGACGCCG ACCGG

Details for MyavA.10611.a.A1.GE29167

HARVESTED ON:	4/27/2010
SEQUENCED ON:	5/1/2010
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVKTKGALI WEFNQPWSIE EIEIGDPQAH EVKIQMEAAG MCHSDHHLVT GGIPMAGFPV LGGHEGAGIV TEVGPGVEDI APGDHVVLSF IPSCGSCPTC QAGMRNLCDL GAGLLGGEAV SDGTFRIQAR GQNVYPMTLL GTFSPYMVVH RSSVVKIDPS IPFEVAALVG CGVTTGYGSA VRTADIRPGQ DVAIVGVGGV GMAALQGAVN AGARYIFAID PVEWKRDQAL KFGATHVYPD IMAAMAGMAE VTYGLMAHKV VVTVGELQGA DVDNYLNITQ KGGTCVLTAI GSLLDTNVTL NLAMLTLMQK NLQGTIFGGG NPQYDIPQLL SMYKAGKLNL DDMITRQYRL EQINDGYQDM LDGKNIRGVI RYTDADR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagaca aaaggcgcac tgatctggga gttcaaccag ccctggtcca tcgaggaaat cgagatcggc gacccgcaag cgcatgaggt caagatccag atggaagcgg cgggcatgtg ccactccgac catcacctcg tcaccggcgg catcccgatg gccggcttcc cggtgctcgg cgggcacgag ggcgcgggca tcgtcaccga ggtcggcccc ggcgtcgagg acatcgcccc gggcgaccac gtggtgctgt cgttcatccc gtcctgcggc tcgtgtccga cctgccaggc gggcatgcgc aacctgtgtg acctgggtgc gggtctgctg ggcggcgaag cggtgtccga cgggaccttc cgcatccagg cccgcggcca gaacgtctac cccatgacgc tgctgggcac gttttctccg tacatggtgg tgcaccgcag ctcggtggtg aagatcgacc cgtcgatccc gttcgaggtg gccgcgctgg tcggctgcgg cgtcaccacc ggttacgggt cggcggtccg caccgccgac atccggccgg gccaggacgt cgccatcgtc ggcgtcggcg gcgtgggcat ggcggcgctg cagggcgcgg tcaacgccgg cgcccgctac atcttcgcga tcgacccggt ggagtggaag cgcgaccagg cactgaagtt cggcgccacc cacgtctacc ccgacatcat ggccgccatg gccggcatgg ccgaggtgac ctacggcctg atggcccaca aggtcgtcgt caccgtgggc gagctgcagg gcgccgacgt cgacaactac ctgaacatca cccagaaggg cggcacctgc gtgctgaccg ccatcggcag cctgctggac accaacgtga cgctgaacct ggcgatgctg accctgatgc agaagaattt gcagggcacc atctttggtg gcggcaaccc gcagtacgac atcccgcagc tgctgtcgat gtacaaggcc ggcaagctca acctggacga catgatcacc cgccagtacc ggctcgagca gatcaacgac ggctatcagg acatgttgga cggcaagaac attcgcggcg tcatccgcta caccgacgcc gaccggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa