MyabA.10611.a

Alcohol dehydrogenase B (AdhB)

CENTER ID:	MyabA.10611.a
ORGANISM:	Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE:	Skin abscess
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyabA.10611.a.A1.GE30546	Full length( MyabA.10611.a )		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127257
PATRIC ID:	fig\|36809.5.peg.4634
RefSeq:	YP_001705283.1
UniProt:	B1MKY4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MYLEGLAESM KTKGALIREL NKPIVVEEIT FGDPAEGEVQ VQLHAAGMCH SDYHVLTGAT PMELPVLPGH EGAGVVTKVG KGVTSVQEGD HVIFAFIPAC GKCPPCMRGY RSLCDRGAIL LGGKAIADGT NRIHAGNTPV SAMNLLGTFS PYAVVHEDSV VKIDDDVPFE SAALLGCAVP TGFGSSTNIA EVKPGEDVII VGIGGIGMSA LQGAVVSGAR RVIAIDPVPW KREQALKFGA THAFPTMAEA LLPVMDLTWG IMAQKTIITV GEMRGEMIEE AMLLTAKTGT CVVTGMGAMV DVDVKFNLFL FTMLQKTLKG NIFGGGTTHT ETPRLVGLYK QGLLKIDEMI TSTYSLEQIN EGYQDMLDGK NIRGVIKYTE ADW

NT Sequence

GTGTATCTGG AAGGACTAGC GGAGTCAATG AAGACAAAAG GCGCGCTGAT TCGCGAGCTC AACAAGCCGA TCGTCGTCGA AGAGATCACG TTCGGTGATC CGGCCGAAGG CGAGGTCCAG GTCCAGCTGC ACGCCGCAGG CATGTGCCAC TCCGATTACC ACGTCCTGAC CGGCGCCACC CCGATGGAGC TGCCGGTGCT GCCCGGCCAC GAGGGCGCGG GCGTGGTCAC CAAGGTGGGC AAGGGTGTCA CTTCCGTCCA GGAAGGCGAC CACGTGATCT TCGCCTTCAT CCCGGCCTGC GGTAAGTGCC CGCCGTGTAT GCGCGGCTAC CGATCGCTAT GCGATCGCGG TGCGATTCTG CTGGGCGGCA AGGCTATTGC CGATGGCACC AACCGCATCC ACGCGGGCAA CACTCCCGTC TCCGCGATGA ACCTGCTCGG CACCTTCTCG CCCTACGCCG TGGTGCATGA GGACTCCGTG GTCAAGATCG ATGACGACGT GCCCTTCGAA TCGGCCGCGC TCCTCGGCTG CGCCGTGCCC ACCGGCTTCG GCTCGTCCAC CAACATCGCC GAGGTCAAAC CGGGCGAAGA CGTGATCATC GTCGGCATCG GCGGCATCGG CATGAGCGCC CTACAGGGCG CCGTCGTCTC CGGCGCCCGC CGTGTCATCG CGATCGACCC GGTGCCGTGG AAGCGCGAAC AGGCCCTCAA GTTCGGCGCC ACCCACGCCT TCCCCACCAT GGCCGAGGCA CTGCTGCCCG TCATGGACCT GACCTGGGGC ATCATGGCCC AGAAGACCAT CATCACCGTC GGCGAGATGC GCGGCGAGAT GATCGAAGAG GCCATGCTGC TGACCGCCAA GACCGGCACG TGCGTGGTGA CGGGCATGGG CGCCATGGTC GACGTGGACG TCAAGTTCAA CCTGTTCCTC TTCACCATGC TGCAGAAGAC CTTGAAGGGC AACATCTTTG GCGGCGGCAC CACGCACACC GAGACCCCGC GCCTGGTCGG CCTGTACAAA CAGGGCCTGC TCAAGATCGA CGAGATGATC ACCAGCACCT ACAGCCTGGA GCAGATCAAC GAGGGATACC AGGACATGCT CGACGGCAAG AACATCCGCG GCGTCATCAA GTACACCGAA GCCGACTGG

Details for MyabA.10611.a.A1.GE30546

HARVESTED ON:	9/21/2010
SEQUENCED ON:	9/28/2010
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	79
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVYLEGLAE SMKTKGALIR ELNKPIVVEE ITFGDPAEGE VQVQLHAAGM CHSDYHVLTG ATPMELPVLP GHEGAGVVTK VGKGVTSVQE GDHVIFAFIP ACGKCPPCMR GYRSLCDRGA ILLGGKAIAD GTNRIHAGNT PVSAMNLLGT FSPYAVVHED SVVKIDDDVP FESAALLGCA VPTGFGSSTN IAEVKPGEDV IIVGIGGIGM SALQGAVVSG ARRVIAIDPV PWKREQXLKF GATHAFPTMX XALLPVMDLT WGIMAXKTII TVGXMRGEMI EXXMLLTAXT GTCVXTGGRH GRXGXQXQXX XLXHAAXXXX RATXXXXGXX XXXXXXXXXX XXXXXXXXIX XXXXXXXXXX XXXXXXXXXX

Validated NT Sequence

attttgttta ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt atctggaagg actagcggag tcaatgaaga caaaaggcgc gctgattcgc gagctcaaca agccgatcgt cgtcgaagag atcacgttcg gtgatccggc cgaaggcgag gtccaggtcc agctgcacgc cgcaggcatg tgccactccg attaccacgt cctgaccggc gccaccccga tggagctgcc ggtgctgccc ggccacgagg gcgcgggcgt ggtcaccaag gtgggcaagg gtgtcacttc cgtccaggaa ggcgaccacg tgatcttcgc cttcatcccg gcctgcggta agtgcccgcc gtgtatgcgc ggctaccgat cgctatgcga tcgcggtgcg attctgctgg gcggcaaggc tattgccgat ggcaccaacc gcatccacgc gggcaacact cccgtctccg cgatgaacct gctcggcacc ttctcgccct acgccgtggt gcatgaggac tccgtggtca agatcgatga cgacgtgccc ttcgaatcgg ccgcgctcct cggctgcgcc gtgcccaccg gcttcggctc gtccaccaac atcgccgagg tcaaaccggg cgaagacgtg atcatcgtcg gcatcggcgg catcggcatg agcgccctac agggcgccgt cgtctccggc gcccgccgtg tcatcgcgat cgacccggtg ccgtggaagc gcgaacaggn cctcaagttc ggcgccaccc acgccttccc caccatggnc gangcactgc tgcccgtcat ggacctgacc tggggcatca tggcccanaa gaccatcatc accgtcggcn anatgcgcgg cgagatgatc gaagangnca tgctgctgac cgccaanacc ggnacgtgcg tgnngacngg tgggcgccat ggncgacnng gnngtcaant ncaanctnnn cntcttnncc atgctgcnnn angannntnn agggcaacat nntnngnngn cggnncncnn nnnncncnnn nnnnggnnnn nnnnnnnaan nngnnnnnnn naannnnnnn nnnnatcncn nnnncnnnnn nnnnnnnnnn nnannnngnn nnnnnnnnnn ngnnnnnnnn nnnnnnnnnn ngg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVYLEGLAE SMKTKGALIR ELNKPIVVEE ITFGDPAEGE VQVQLHAAGM CHSDYHVLTG ATPMELPVLP GHEGAGVVTK VGKGVTSVQE GDHVIFAFIP ACGKCPPCMR GYRSLCDRGA ILLGGKAIAD GTNRIHAGNT PVSAMNLLGT FSPYAVVHED SVVKIDDDVP FESAALLGCA VPTGFGSSTN IAEVKPGEDV IIVGIGGIGM SALQGAVVSG ARRVIAIDPV PWKREQALKF GATHAFPTMA EALLPVMDLT WGIMAQKTII TVGEMRGEMI EEAMLLTAKT GTCVVTGMGA MVDVDVKFNL FLFTMLQKTL KGNIFGGGTT HTETPRLVGL YKQGLLKIDE MITSTYSLEQ INEGYQDMLD GKNIRGVIKY TEADW

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtatctg gaaggactag cggagtcaat gaagacaaaa ggcgcgctga ttcgcgagct caacaagccg atcgtcgtcg aagagatcac gttcggtgat ccggccgaag gcgaggtcca ggtccagctg cacgccgcag gcatgtgcca ctccgattac cacgtcctga ccggcgccac cccgatggag ctgccggtgc tgcccggcca cgagggcgcg ggcgtggtca ccaaggtggg caagggtgtc acttccgtcc aggaaggcga ccacgtgatc ttcgccttca tcccggcctg cggtaagtgc ccgccgtgta tgcgcggcta ccgatcgcta tgcgatcgcg gtgcgattct gctgggcggc aaggctattg ccgatggcac caaccgcatc cacgcgggca acactcccgt ctccgcgatg aacctgctcg gcaccttctc gccctacgcc gtggtgcatg aggactccgt ggtcaagatc gatgacgacg tgcccttcga atcggccgcg ctcctcggct gcgccgtgcc caccggcttc ggctcgtcca ccaacatcgc cgaggtcaaa ccgggcgaag acgtgatcat cgtcggcatc ggcggcatcg gcatgagcgc cctacagggc gccgtcgtct ccggcgcccg ccgtgtcatc gcgatcgacc cggtgccgtg gaagcgcgaa caggccctca agttcggcgc cacccacgcc ttccccacca tggccgaggc actgctgccc gtcatggacc tgacctgggg catcatggcc cagaagacca tcatcaccgt cggcgagatg cgcggcgaga tgatcgaaga ggccatgctg ctgaccgcca agaccggcac gtgcgtggtg acgggcatgg gcgccatggt cgacgtggac gtcaagttca acctgttcct cttcaccatg ctgcagaaga ccttgaaggg caacatcttt ggcggcggca ccacgcacac cgagaccccg cgcctggtcg gcctgtacaa acagggcctg ctcaagatcg acgagatgat caccagcacc tacagcctgg agcagatcaa cgagggatac caggacatgc tcgacggcaa gaacatccgc ggcgtcatca agtacaccga agccgactgg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa