MymaA.10611.a

Zinc-containing alcohol dehydrogenase NAD-dependent

CENTER ID:	MymaA.10611.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.10611.a.A1.GE30236	Full length( MymaA.10611.a )		1	368

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127257
PATRIC ID:	fig\|216594.6.peg.3369
RefSeq:	YP_001851439.1
UniProt:	B2HG20

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTRAAVLWG LGEKWEVEDI ELDPPGPDEV LVQLTATGLC HSDEHLVTGD LPIPLPVVGG HEGAGTVVEV GAGVENVAEG DSVILTFLPS CGHCSYCARG MGNMCDLGAA LMMGPQIDGT YRFHARGEDV GQMCLLGTFS EYTVVPKASL VKIDQGTPLD KAALIGCGVT TGYGSAVRTG EVRAGDTVAV IGAGGIGMNA IQGARIAGAL NIVAVDPVAF KREQAGGFGA THAVATIDEA WSLISDITRG KLADVCVLTT DVAEGSYIGE ALSLVGKRGR VVVTAIGHPE DTSMSGSLLE LTLYEKQIRG ALYGSSNAAH DIPRLVELYN AGHLKLDELI TREYTLDQIN EGYADMRSGR NIRGLIRF

NT Sequence

ATGAAGACAC GTGCTGCAGT GCTGTGGGGC CTAGGAGAAA AGTGGGAAGT CGAGGACATC GAGTTGGATC CTCCCGGCCC CGATGAAGTG CTCGTCCAGC TGACCGCGAC CGGGTTGTGC CATTCCGACG AACACCTCGT GACCGGCGAC CTGCCCATTC CGCTGCCCGT CGTCGGTGGC CACGAGGGTG CCGGCACCGT GGTCGAAGTG GGTGCGGGTG TCGAGAACGT CGCCGAGGGC GATTCGGTGA TCCTGACGTT CCTGCCGTCG TGCGGGCACT GTTCCTATTG CGCGCGGGGG ATGGGGAACA TGTGCGACTT GGGTGCCGCG CTCATGATGG GACCCCAGAT CGACGGCACT TACCGTTTCC ATGCCAGGGG TGAGGACGTC GGCCAGATGT GCCTGCTGGG CACGTTCTCG GAATACACCG TGGTCCCCAA GGCGTCTCTG GTCAAAATCG ACCAGGGGAC ACCGCTGGAC AAGGCGGCGT TGATCGGGTG TGGTGTGACG ACCGGGTACG GGTCGGCGGT GCGCACCGGT GAGGTGCGTG CCGGGGATAC CGTGGCGGTG ATCGGAGCCG GTGGCATCGG CATGAATGCG ATTCAGGGTG CCCGCATCGC GGGCGCGTTG AACATCGTGG CTGTCGATCC GGTGGCGTTC AAGCGTGAAC AAGCCGGCGG TTTCGGTGCG ACGCATGCCG TTGCCACGAT CGACGAGGCC TGGTCACTGA TCAGTGACAT CACCCGCGGC AAACTCGCCG ATGTCTGCGT CTTGACCACC GATGTCGCCG AAGGGTCCTA CATCGGCGAG GCGCTGTCCT TGGTCGGTAA ACGCGGCCGT GTGGTCGTTA CCGCGATCGG GCACCCCGAA GACACCTCGA TGTCGGGTTC ACTGCTGGAA TTGACGCTGT ATGAAAAGCA GATCCGCGGC GCTCTGTACG GTTCTTCGAA CGCCGCCCAC GACATCCCGC GGCTCGTCGA GCTCTACAAC GCCGGACACC TCAAGCTGGA TGAGCTGATC ACCCGTGAGT ACACCCTCGA TCAGATCAAC GAAGGCTACG CGGATATGCG ATCGGGCCGC AACATCCGAG GACTCATCCG ATTC

Details for MymaA.10611.a.A1.GE30236

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	40kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	89
PERCENT COVERAGE:	82

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKTRAAVLW GLGEKWEVED IELDPPGPDE VLVQLTATGL CHSDEHLVTG DLPIPLPVVG GHEGAGTVVE VGAGVENVAE GDSVILTFLP SCGHCSYCAR GMGNMCDLGA ALMMGPQIDG TYRFHARGED VGQMCLLGTF SEYTVVPKAS LVKIDQGTPL DKAALIGCGV TTGYGSAVRT GEVRAGDTVA VIGAGGIGMN AIQGARIAGA LNIVAVDPVA FKREQAGGFG ATHAVATIDE AWSLISDITR GKLAXVCVLT TDVAXXPTSA RRXXWSXNAA VWSLPXSGTX XXPXCXXHCX X

Validated NT Sequence

annnnntttn tttactttaa gaaggagana taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg aagacacgtg ctgcagtgct gtggggccta ggagaaaagt gggaagtcga ggacatcgag ttggatcctc ccggccccga tgaagtgctc gtccagctga ccgcgaccgg gttgtgccat tccgacgaac acctcgtgac cggcgacctg cccattccgc tgcccgtcgt cggtggccac gagggtgccg gcaccgtggt cgaagtgggt gcgggtgtcg agaacgtcgc cgagggcgat tcggtgatcc tgacgttcct gccgtcgtgc gggcactgtt cctattgcgc gcgggggatg gggaacatgt gcgacttggg tgccgcgctc atgatgggac cccagatcga cggcacttac cgtttccatg ccaggggtga ggacgtcggc cagatgtgcc tgctgggcac gttctcggaa tacaccgtgg tccccaaggc gtctctggtc aaaatcgacc aggggacacc gctggacaag gcggcgttga tcgggtgtgg tgtgacgacc gggtacgggt cggcggtgcg caccggtgag gtgcgtgccg gggataccgt ggcggtgatc ggagccggtg gcatcggcat gaatgcgatt cagggtgccc gcatcgcggg cgcgttgaac atcgtggctg tcgatccggt ggcgttcaag cgtgaacaag ccggcggttt cggtgcgacg catgccgttg ccacgatcga cgaggcctgg tcactgatca gtgacatcac ccgcggcaaa ctcgccgang tctgcgtctt gaccaccgat gtcgccgann nncctacntc ggcnaggcgc tgnncttggt cggnaaacgc ngccgtgtgg tcgttaccgc natcgggnac ccngaannnn ccnnnatgtn ngnttcactg ctnnnattga ngctgtatga aaagcanann cncnncgctc ngnangtnnn nnaannnnnc cacnacntnc nnnnnntcgn cnnnctnnac annanncnnn ngnnnnngnn ntgatcnccn nnnnnnnnnn ccnnnnnncn nnnnnnnnnn nnnnnannnn nnnnngnnnn nnnnnncnnn nnnnnnnnnn nnnnannann tnnnnnnnnn nnnnnnnnnn tnnnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKTRAAVLW GLGEKWEVED IELDPPGPDE VLVQLTATGL CHSDEHLVTG DLPIPLPVVG GHEGAGTVVE VGAGVENVAE GDSVILTFLP SCGHCSYCAR GMGNMCDLGA ALMMGPQIDG TYRFHARGED VGQMCLLGTF SEYTVVPKAS LVKIDQGTPL DKAALIGCGV TTGYGSAVRT GEVRAGDTVA VIGAGGIGMN AIQGARIAGA LNIVAVDPVA FKREQAGGFG ATHAVATIDE AWSLISDITR GKLADVCVLT TDVAEGSYIG EALSLVGKRG RVVVTAIGHP EDTSMSGSLL ELTLYEKQIR GALYGSSNAA HDIPRLVELY NAGHLKLDEL ITREYTLDQI NEGYADMRSG RNIRGLIRF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagacacgt gctgcagtgc tgtggggcct aggagaaaag tgggaagtcg aggacatcga gttggatcct cccggccccg atgaagtgct cgtccagctg accgcgaccg ggttgtgcca ttccgacgaa cacctcgtga ccggcgacct gcccattccg ctgcccgtcg tcggtggcca cgagggtgcc ggcaccgtgg tcgaagtggg tgcgggtgtc gagaacgtcg ccgagggcga ttcggtgatc ctgacgttcc tgccgtcgtg cgggcactgt tcctattgcg cgcgggggat ggggaacatg tgcgacttgg gtgccgcgct catgatggga ccccagatcg acggcactta ccgtttccat gccaggggtg aggacgtcgg ccagatgtgc ctgctgggca cgttctcgga atacaccgtg gtccccaagg cgtctctggt caaaatcgac caggggacac cgctggacaa ggcggcgttg atcgggtgtg gtgtgacgac cgggtacggg tcggcggtgc gcaccggtga ggtgcgtgcc ggggataccg tggcggtgat cggagccggt ggcatcggca tgaatgcgat tcagggtgcc cgcatcgcgg gcgcgttgaa catcgtggct gtcgatccgg tggcgttcaa gcgtgaacaa gccggcggtt tcggtgcgac gcatgccgtt gccacgatcg acgaggcctg gtcactgatc agtgacatca cccgcggcaa actcgccgat gtctgcgtct tgaccaccga tgtcgccgaa gggtcctaca tcggcgaggc gctgtccttg gtcggtaaac gcggccgtgt ggtcgttacc gcgatcgggc accccgaaga cacctcgatg tcgggttcac tgctggaatt gacgctgtat gaaaagcaga tccgcggcgc tctgtacggt tcttcgaacg ccgcccacga catcccgcgg ctcgtcgagc tctacaacgc cggacacctc aagctggatg agctgatcac ccgtgagtac accctcgatc agatcaacga aggctacgcg gatatgcgat cgggccgcaa catccgagga ctcatccgat tcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa