MythA.10611.b

Alcohol dehydrogenase B (A0QNZ4 homolog)

CENTER ID:	MythA.10611.b
ORGANISM:	Mycobacterium thermoresistibile ATCC 19527 / NCTC 10409
ASSOCIATED DISEASE:	Respiratory Infection
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MythA.10611.b.A1.GE29592	Full length( MythA.10611.b )		1	374

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1078020.3.peg.1044
RefSeq:	WP_003924529.1
UniProt:	G7CDJ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTKGALLWE LNSPFRVDEI DVGDPVEDEV QIRLHAAGMC HSDYHLTTGA TPIGLPALGG HEGAGVITKV GKNVTGLQEG DHVILAFIPS CGQCQPCLRG HRSLCDRGAV LLGGKAIADG TSRVRAGSTE VSPMNLLGTF APYMTVHKDS VVKIDPEVPF ETAAIMGCAV PTGFGSATNV AQVQPGETVV IVGVGGIGLS ALQGAVISGA KHVIAIDPVE FKRDTALKFG ATHVFSSMAE AINPVLELTH GFMAEKTIIA VGEMRGEYIE EAMTLTAKTG TCVVTGMGAM TDVDVKLNLF LFTMLQKTLK GNIFGGGSSH VETPRLVALY KSGLLKIDEM VTNTYRLEDI NQGYQDMLDG KNIRGVIVYD ESDW

NT Sequence

ATGAAGACCA AAGGCGCACT GCTGTGGGAG CTGAACTCGC CGTTCCGGGT CGACGAGATC GACGTCGGTG ACCCCGTCGA GGACGAGGTG CAGATCCGGC TGCATGCCGC GGGCATGTGC CACTCCGACT ACCACCTGAC CACCGGCGCC ACCCCGATCG GCCTGCCGGC GTTGGGCGGG CACGAGGGCG CCGGGGTCAT CACCAAGGTC GGCAAGAACG TCACCGGCCT ACAGGAGGGC GACCACGTCA TCCTGGCGTT CATCCCGTCC TGCGGCCAGT GCCAGCCGTG TCTGCGCGGC CACCGCTCGT TGTGCGACCG GGGCGCGGTG CTGCTGGGCG GCAAGGCGAT CGCCGACGGC ACCTCCCGGG TGCGTGCCGG CAGCACCGAG GTGTCGCCGA TGAACCTGCT CGGCACCTTC GCCCCCTACA TGACCGTGCA CAAGGACTCG GTCGTCAAGA TCGACCCGGA GGTGCCGTTC GAGACCGCGG CGATCATGGG CTGCGCGGTG CCGACGGGCT TCGGGTCGGC CACCAACGTC GCGCAGGTCC AGCCGGGTGA GACGGTCGTC ATCGTCGGTG TCGGCGGGAT CGGCCTGAGC GCGCTGCAGG GCGCGGTGAT CTCCGGCGCC AAGCACGTCA TCGCGATCGA CCCGGTGGAG TTCAAGCGCG ACACCGCGCT GAAATTCGGT GCCACCCATG TCTTCTCGTC AATGGCCGAG GCGATCAACC CGGTTCTGGA GCTGACCCAC GGGTTCATGG CCGAGAAGAC CATCATCGCG GTCGGCGAGA TGCGCGGCGA GTACATCGAA GAGGCGATGA CGCTGACCGC CAAGACCGGC ACCTGCGTGG TCACCGGCAT GGGCGCGATG ACCGACGTCG ACGTCAAGCT CAACCTGTTC CTGTTCACCA TGCTGCAGAA GACGTTGAAG GGCAACATCT TCGGTGGCGG CAGCTCGCAC GTGGAGACGC CGCGGCTGGT GGCGCTGTAC AAGTCCGGGT TGCTCAAGAT CGACGAGATG GTGACCAACA CCTACCGGCT TGAGGACATC AACCAGGGCT ACCAGGACAT GCTCGACGGG AAGAACATCC GCGGCGTCAT CGTCTACGAC GAATCGGACT GG

Details for MythA.10611.b.A1.GE29592

HARVESTED ON:	6/22/2010
SEQUENCED ON:	7/1/2010
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

ccgattcgtc gtagacgatg acgccgcgga tgttcttccc gtcgagcatg tcctggtagc cctggttgat gtcctcaagc cggtaggtgt tggtcaccat ctcgtcgatc ttgagcaacc cggacttgta cagcgccacc agccgcggcg tctccacgtg cgagctgccg ccaccgaaga tgttgccctt caacgtcttc tgcagcatgg tgaacaggaa caggttgagc ttgacgtcga cgtcggtcat cgcgcccatg ccggtgacca cgcaggtgcc ggtcttggcg gtcagcgtca tcgcctcttc gatgtactcg ccgcgcatct cgccgaccgc gatgatggtc ttctcggcca tgaacccgtg ggtcagctcc agaaccgggt tgatcgcctc ggccattgac gagaagacat gggtggcacc gaatttcagc gcggtgtcgc gcttgaactc caccgggtcg atcgcgatga cgtgcttggc gccggagatc accgcgccct gcagcgcgct caggccgatc ccgccgacac cgacgatgac gaccgtctca cccggctgga cctgcgcgac gttggtggcc gacccgaagc ccgtcggcac cgcgcagccc atgatcgccg cggtctcgaa cggcacctcc gggtcgatct tgacgaccga gtccttgtgc acggtcatgt agggggcgaa ggtgccgagc aggttcatcg gcgacacctc ggtgctgccg gcacgcaccc gggaggtgcc gtcggcgatc gccttgccgc ccagcagcac cgcgccccgg tcgcacaacg agcggtggcc gcgcagacac ggctggcact ggccgcagga cgggatgaac gccaggatga cgtggtcgcc ctcctgtagg ccggtgacgt tcttgccgac cttggtgatg accccggcgc cctcgtgccc gcccaacgcc ggcaggccga tcggggtggc gccggtggtc aggtggtagt cggagtggca catgcccgcg gcatgcagcc ggatctgcac ctcgtcctcg acggggtcac cgacgtcgat ctcgtcgacc cggaacggcg agttcagctc ccacagcagt gcgcctttgg tcttcatcga accaggaccc tgggtctgag cttccagggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMKTKGALLW ELNSPFRVDE IDVGDPVEDE VQIRLHAAGM CHSDYHLTTG ATPIGLPALG GHEGAGVITK VGKNVTGLQE GDHVILAFIP SCGQCQPCLR GHRSLCDRGA VLLGGKAIAD GTSRVRAGST EVSPMNLLGT FAPYMTVHKD SVVKIDPEVP FETAAIMGCA VPTGFGSATN VAQVQPGETV VIVGVGGIGL SALQGAVISG AKHVIAIDPV EFKRDTALKF GATHVFSSMA EAINPVLELT HGFMAEKTII AVGEMRGEYI EEAMTLTAKT GTCVVTGMGA MTDVDVKLNL FLFTMLQKTL KGNIFGGGSS HVETPRLVAL YKSGLLKIDE MVTNTYRLED INQGYQDMLD GKNIRGVIVY DESDW

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagaccaaa ggcgcactgc tgtgggagct gaactcgccg ttccgggtcg acgagatcga cgtcggtgac cccgtcgagg acgaggtgca gatccggctg catgccgcgg gcatgtgcca ctccgactac cacctgacca ccggcgccac cccgatcggc ctgccggcgt tgggcgggca cgagggcgcc ggggtcatca ccaaggtcgg caagaacgtc accggcctac aggagggcga ccacgtcatc ctggcgttca tcccgtcctg cggccagtgc cagccgtgtc tgcgcggcca ccgctcgttg tgcgaccggg gcgcggtgct gctgggcggc aaggcgatcg ccgacggcac ctcccgggtg cgtgccggca gcaccgaggt gtcgccgatg aacctgctcg gcaccttcgc cccctacatg accgtgcaca aggactcggt cgtcaagatc gacccggagg tgccgttcga gaccgcggcg atcatgggct gcgcggtgcc gacgggcttc gggtcggcca ccaacgtcgc gcaggtccag ccgggtgaga cggtcgtcat cgtcggtgtc ggcgggatcg gcctgagcgc gctgcagggc gcggtgatct ccggcgccaa gcacgtcatc gcgatcgacc cggtggagtt caagcgcgac accgcgctga aattcggtgc cacccatgtc ttctcgtcaa tggccgaggc gatcaacccg gttctggagc tgacccacgg gttcatggcc gagaagacca tcatcgcggt cggcgagatg cgcggcgagt acatcgaaga ggcgatgacg ctgaccgcca agaccggcac ctgcgtggtc accggcatgg gcgcgatgac cgacgtcgac gtcaagctca acctgttcct gttcaccatg ctgcagaaga cgttgaaggg caacatcttc ggtggcggca gctcgcacgt ggagacgccg cggctggtgg cgctgtacaa gtccgggttg ctcaagatcg acgagatggt gaccaacacc taccggcttg aggacatcaa ccagggctac caggacatgc tcgacgggaa gaacatccgc ggcgtcatcg tctacgacga atcggactgg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa