MyavA.10611.c

Alcohol dehydrogenase B

CENTER ID: MyavA.10611.c
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.10611.c.A1.GE29169 Full length( MyavA.10611.c ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127257
PATRIC ID: fig|243243.7.peg.5118
RefSeq: YP_884330.1
UniProt: A0A0H3A4F3
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIRGVGRDWE IAEIELDPPR SGEVLVRMAV AGICHSDDHL FTGDVVPTPE VVAASGQPAP DWFPLLGGHE GAGVVAEVGP NVTSVRPGDH VALSFIPACG SCRFCVNGQS YICDVGASLF VREMPTDGTC RRHLGAENLL AYGQLGTFAE YAVLSERSVI KIDEAIPFHA ASLVSCGVST GWGSATVSAG TEPGDTVVVI GAGGVGMNAL QGARAVGAKY VVAVDPIAAK RDSAKIFGAT HTAASARDAI PLVREITAGL MADRVVVCPG VVHVDSIPLA LALLRKGGVC VLTGITPFTE PPVPLVLQEM TLSAKQLRGA LYGGMNPRTS VPMLLSLYQA GDLKLDELVT RHYRLDEINE AFADLREGRN IRGVIDFAGR
NT Sequence
GTGATCCGCG GGGTCGGCCG GGACTGGGAG ATCGCCGAGA TCGAGCTCGA CCCGCCGCGC AGCGGTGAGG TGCTGGTGCG GATGGCCGTC GCCGGGATCT GCCACTCCGA CGACCATCTG TTCACCGGTG ACGTGGTGCC GACGCCCGAG GTGGTCGCCG CGAGCGGTCA GCCCGCGCCG GACTGGTTCC CGCTGCTCGG CGGTCACGAG GGCGCCGGTG TCGTCGCGGA AGTCGGGCCC AACGTGACGT CGGTGCGCCC GGGCGACCAC GTGGCGCTGT CGTTCATCCC GGCGTGCGGC AGCTGCCGGT TCTGCGTGAA CGGCCAGAGC TACATCTGCG ACGTCGGCGC CAGCCTGTTC GTGCGCGAGA TGCCCACCGA CGGGACCTGC CGCCGCCATC TCGGTGCGGA AAACCTGTTG GCCTACGGGC AACTCGGCAC CTTCGCCGAG TACGCGGTGC TCTCGGAACG GTCCGTCATC AAGATCGACG AGGCGATTCC GTTCCATGCC GCCTCCCTGG TGTCGTGCGG GGTCAGCACC GGATGGGGCT CGGCGACGGT GTCGGCGGGC ACCGAACCGG GCGACACCGT GGTGGTGATC GGCGCCGGCG GTGTCGGGAT GAACGCGCTG CAGGGCGCCC GCGCGGTGGG CGCGAAGTAC GTCGTCGCGG TGGATCCCAT TGCAGCCAAA CGGGATTCGG CCAAGATCTT CGGCGCCACG CACACCGCCG CGTCCGCGCG GGACGCCATA CCGCTGGTCA GGGAGATCAC CGCCGGCCTG ATGGCCGACC GGGTGGTGGT CTGTCCCGGC GTGGTGCACG TGGACTCGAT CCCGTTGGCG CTGGCGCTGC TGCGCAAGGG CGGGGTCTGC GTGCTGACCG GCATCACCCC GTTCACCGAG CCCCCGGTTC CGCTGGTGCT GCAGGAGATG ACGCTGTCGG CCAAACAACT GCGGGGCGCC CTCTACGGCG GGATGAATCC GCGCACCAGC GTGCCGATGC TGTTGTCCCT GTATCAGGCG GGCGATTTGA AACTCGACGA GTTGGTCACC CGCCACTACC GGCTCGACGA GATCAACGAG GCATTCGCCG ACCTGCGCGA GGGCCGCAAC ATCCGCGGCG TCATCGACTT CGCCGGCCGG
Details for MyavA.10611.c.A1.GE29169
HARVESTED ON: 4/27/2010
SEQUENCED ON: 5/1/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Validated AA Sequence
MAVAGICHSD DHLFTGDVVP TPEVVAASGQ PAPDWFPLLG GHEGAGVVAE VGPNVTSVRP GDHVALSFIP ACGSCRFCVN GQSYICDVGA SLFVREMPTD GTCRRHLGAE NLLAYGQLGT FAEYAVLSER SVIKIDEAIP FHAASLVSCG VSTGWGSATV SAGTEPGDTV VVIGAGGVGM NALQGARAVG AKYVVAVDPI AAKRDSAKIF GATHTAASAR DAIPLVREIT AGLMADRVVV CPGVVHVDSI PLALALLRKG GVCVLTGITP FTEPPVPLVL QEMTLSAKQL RGALYGGMNP RTSVPMLLSL YQAGDLKLDE LVTRHYRLDE INEAFADLRE GRNIRGVIDF AGR
Validated NT Sequence
gggagatcgc cgagatcgag ctcgacccgc cgcgcagcgg tgaggtgctg gtgcggatgg ccgtcgccgg gatctgccac tccgacgacc atctgttcac cggtgacgtg gtgccgacgc ccgaggtggt cgccgcgagc ggtcagcccg cgccggactg gttcccgctg ctcggcggtc acgagggcgc cggtgtcgtc gcggaagtcg ggcccaacgt gacgtcggtg cgcccgggcg accacgtggc gctgtcgttc atcccggcgt gcggcagctg ccggttctgc gtgaacggcc agagctacat ctgcgacgtc ggcgccagcc tgttcgtgcg cgagatgccc accgacggga cctgccgccg ccatctcggt gcggaaaacc tgttggccta cgggcaactc ggcaccttcg ccgagtacgc ggtgctctcg gaacggtccg tcatcaagat cgacgaggcg attccgttcc atgccgcctc cctggtgtcg tgcggggtca gcaccggatg gggctcggcg acggtgtcgg cgggcaccga accgggcgac accgtggtgg tgatcggcgc cggcggtgtc gggatgaacg cgctgcaggg cgcccgcgcg gtgggcgcga agtacgtcgt cgcggtggat cccattgcag ccaaacggga ttcggccaag atcttcggcg ccacgcacac cgccgcgtcc gcgcgggacg ccataccgct ggtcagggag atcaccgccg gcctgatggc cgaccgggtg gtggtctgtc ccggcgtggt gcacgtggac tcgatcccgt tggcgctggc gctgctgcgc aagggcgggg tctgcgtgct gaccggcatc accccgttca ccgagccccc ggttccgctg gtgctgcagg agatgacgct gtcggccaaa caactgcggg gcgccctcta cggcgggatg aatccgcgca ccagcgtgcc gatgctgttg tccctgtatc aggcgggcga tttgaaactc gacgagttgg tcacccgcca ctaccggctc gacgagatca acgaggcatt cgccgacctg cgcgagggcc gcaacatccg cggcgtcatc gacttcgccg gccggtaat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIRGVGRD WEIAEIELDP PRSGEVLVRM AVAGICHSDD HLFTGDVVPT PEVVAASGQP APDWFPLLGG HEGAGVVAEV GPNVTSVRPG DHVALSFIPA CGSCRFCVNG QSYICDVGAS LFVREMPTDG TCRRHLGAEN LLAYGQLGTF AEYAVLSERS VIKIDEAIPF HAASLVSCGV STGWGSATVS AGTEPGDTVV VIGAGGVGMN ALQGARAVGA KYVVAVDPIA AKRDSAKIFG ATHTAASARD AIPLVREITA GLMADRVVVC PGVVHVDSIP LALALLRKGG VCVLTGITPF TEPPVPLVLQ EMTLSAKQLR GALYGGMNPR TSVPMLLSLY QAGDLKLDEL VTRHYRLDEI NEAFADLREG RNIRGVIDFA GR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatccgc ggggtcggcc gggactggga gatcgccgag atcgagctcg acccgccgcg cagcggtgag gtgctggtgc ggatggccgt cgccgggatc tgccactccg acgaccatct gttcaccggt gacgtggtgc cgacgcccga ggtggtcgcc gcgagcggtc agcccgcgcc ggactggttc ccgctgctcg gcggtcacga gggcgccggt gtcgtcgcgg aagtcgggcc caacgtgacg tcggtgcgcc cgggcgacca cgtggcgctg tcgttcatcc cggcgtgcgg cagctgccgg ttctgcgtga acggccagag ctacatctgc gacgtcggcg ccagcctgtt cgtgcgcgag atgcccaccg acgggacctg ccgccgccat ctcggtgcgg aaaacctgtt ggcctacggg caactcggca ccttcgccga gtacgcggtg ctctcggaac ggtccgtcat caagatcgac gaggcgattc cgttccatgc cgcctccctg gtgtcgtgcg gggtcagcac cggatggggc tcggcgacgg tgtcggcggg caccgaaccg ggcgacaccg tggtggtgat cggcgccggc ggtgtcggga tgaacgcgct gcagggcgcc cgcgcggtgg gcgcgaagta cgtcgtcgcg gtggatccca ttgcagccaa acgggattcg gccaagatct tcggcgccac gcacaccgcc gcgtccgcgc gggacgccat accgctggtc agggagatca ccgccggcct gatggccgac cgggtggtgg tctgtcccgg cgtggtgcac gtggactcga tcccgttggc gctggcgctg ctgcgcaagg gcggggtctg cgtgctgacc ggcatcaccc cgttcaccga gcccccggtt ccgctggtgc tgcaggagat gacgctgtcg gccaaacaac tgcggggcgc cctctacggc gggatgaatc cgcgcaccag cgtgccgatg ctgttgtccc tgtatcaggc gggcgatttg aaactcgacg agttggtcac ccgccactac cggctcgacg agatcaacga ggcattcgcc gacctgcgcg agggccgcaa catccgcggc gtcatcgact tcgccggccg gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa