MymaA.10611.f

Zinc-containing alcohol dehydrogenase NAD dependent AdhB_1

CENTER ID:	MymaA.10611.f
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.10611.f.A1.GE30241	Full length( MymaA.10611.f )		1	384

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127257
PATRIC ID:	fig\|216594.6.peg.4283
RefSeq:	YP_001852260.1
UniProt:	B2HPQ3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRSRAAILHD VGGPWSVEEF ELDTPRTGEV LVEMSAAGLC HTDDHILKGD MSAPNEVLRS RGLPTMFPTI GGHEGSGIVR EIGPGVTDFA PGDHVVMSFI AVCGQCRWCA SGMEYLCDAG IGTLTPGMPT DGTFRHHTAD GTPLGHLAKV GAFAEHTVVS RDSLVKIDPD LPLAPSALLS CAIPTGYGSA ANRAGVRGGD TVVVLGAGGI GTGAIQGARI NGAAQIVAVD PVEFKQKSAH LFGATHGAGS ISEAMALVRE LTHGVMADSV LVSPSLITAD DVRDALRLTR KGGTCVLAGM TSQLTRSVNI DLQEFILMNK TLAGTIFGSC NPRADIARLA GLYQSGQLQL DDMITRRYRL DDINAAYTDL LNGDIVRGII DFTI

NT Sequence

ATGAGAAGCC GGGCGGCCAT CCTGCATGAC GTTGGCGGGC CGTGGTCGGT CGAGGAATTC GAGTTGGACA CACCCCGCAC CGGGGAAGTT CTCGTCGAAA TGTCGGCCGC CGGCTTGTGT CACACTGACG ACCACATCCT CAAGGGTGAC ATGTCCGCCC CGAACGAGGT GCTGCGGTCC CGGGGGCTGC CCACCATGTT CCCAACAATC GGTGGTCACG AAGGCTCGGG CATCGTGCGC GAAATTGGTC CCGGTGTAAC TGATTTCGCG CCCGGCGATC ACGTGGTGAT GTCATTCATC GCGGTATGCG GGCAATGCCG ATGGTGCGCC AGCGGCATGG AGTACCTATG CGATGCCGGC ATCGGCACCC TGACGCCCGG GATGCCCACC GATGGCACGT TCCGGCATCA CACCGCCGAC GGCACGCCAC TGGGCCATCT GGCGAAGGTT GGCGCGTTCG CCGAGCACAC CGTGGTGTCT CGGGATTCAC TGGTCAAGAT CGATCCGGAC CTGCCGCTGG CCCCCAGCGC GTTGCTGTCC TGCGCGATCC CCACCGGGTA CGGGTCGGCA GCGAATCGAG CCGGCGTGCG CGGCGGTGAC ACCGTCGTCG TCCTGGGCGC CGGCGGGATC GGCACGGGGG CCATTCAGGG AGCTCGGATC AATGGCGCCG CGCAGATCGT GGCCGTTGAT CCGGTGGAGT TCAAGCAGAA ATCCGCCCAC CTCTTCGGCG CCACCCACGG CGCCGGATCG ATCAGTGAGG CAATGGCTTT GGTTCGTGAA CTGACTCACG GGGTGATGGC CGACTCGGTA CTGGTCTCGC CGTCGCTGAT CACCGCCGAT GACGTGCGCG ACGCCCTACG GCTCACCCGC AAGGGTGGCA CCTGCGTGCT GGCCGGGATG ACCTCACAAC TGACCCGCTC GGTCAACATC GACCTACAGG AATTCATCTT GATGAACAAG ACCCTGGCCG GCACCATCTT CGGCTCGTGC AACCCCAGGG CCGACATCGC GCGTCTTGCC GGCTTGTATC AGAGCGGACA ACTGCAACTC GACGATATGA TCACCCGCCG CTATCGCCTC GATGACATCA ACGCCGCCTA CACCGACCTG CTCAACGGTG ACATCGTCAG AGGCATAATC GATTTCACGA TC

Details for MymaA.10611.f.A1.GE30241

HARVESTED ON:	8/31/2010
SEQUENCED ON:	9/7/2010
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	67

Validated AA Sequence

Validated NT Sequence

attttnnnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagaa gccgggcggc catcctgcat gacgttggcg ggccgtggtc ggtcgaggaa ttcgagttgg acacaccccg caccggggaa gttctcgtcg aaatgtcggc cgccggcttg tgtcacactg acgaccacat cctcaagggt gacatgtccg ccccgaacga ggtgctgcgg tcccgggggc tgcccaccat gttcccaaca atcggtggtc acgaaggctc gggcatcgtg cgcgaaattg gtcccggtgt aactgatttc gcgcccggcg atcacgtggt gatgtcattc atcgcggtat gcgggcaatg ccgatggtgc gccagcggca tggagtacct atgcgatgcc ggcatcggca ccctgacgcc cgggatgccc accgatggca cgttccggca tcacaccgcc gacggcacgc cactgggcca tctggcgaag gttggcgcgt tcgccgagca caccgtggtg tctcgggatt cactggtcaa gatcgatccg gacctgccgc tggcccccag cgcgttgctg tcctgcgcga tccccaccgg gtacgggtcg gcagcgaatc gagccggcgt gcgcggcggt gacaccgtcg tcgtcctggg cgccggcggg atcggcacgg gggccattca gggagctcgg atcaatggcg ccgcgcanat cgtggccgtt gatccggtgg agttcaagca gaaatccgcc cacctcttcg ncgccaccca cggcgcccgn atcgatcagt gaggcaatgg ctttngttcg tgaactgact cacggggtga tggccgactc ggtactggtc tcnccgtcgc tgatcancgc cgatgacgtg cgcgangncc tannnctnac ccnnnngggt ggcanntgcg tgctggnnnn nnngannnnn nactgacccn ntnnnnnann atcgnnnnac nnnnnatctn ntgaacanna ncnnnnnnnn cntnnnngnt ngnnnnannc cnnnnnnnnn nnnnnngnnn nnnnntnnnt nnnnngnnnn nngcnannnn nnnannnnnn nnnnnnnncn nnnnnnnnnn nnnnccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnannn nnnnnantnn nnnnnnaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRSRAAILH DVGGPWSVEE FELDTPRTGE VLVEMSAAGL CHTDDHILKG DMSAPNEVLR SRGLPTMFPT IGGHEGSGIV REIGPGVTDF APGDHVVMSF IAVCGQCRWC ASGMEYLCDA GIGTLTPGMP TDGTFRHHTA DGTPLGHLAK VGAFAEHTVV SRDSLVKIDP DLPLAPSALL SCAIPTGYGS AANRAGVRGG DTVVVLGAGG IGTGAIQGAR INGAAQIVAV DPVEFKQKSA HLFGATHGAG SISEAMALVR ELTHGVMADS VLVSPSLITA DDVRDALRLT RKGGTCVLAG MTSQLTRSVN IDLQEFILMN KTLAGTIFGS CNPRADIARL AGLYQSGQLQ LDDMITRRYR LDDINAAYTD LLNGDIVRGI IDFTI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagaagccgg gcggccatcc tgcatgacgt tggcgggccg tggtcggtcg aggaattcga gttggacaca ccccgcaccg gggaagttct cgtcgaaatg tcggccgccg gcttgtgtca cactgacgac cacatcctca agggtgacat gtccgccccg aacgaggtgc tgcggtcccg ggggctgccc accatgttcc caacaatcgg tggtcacgaa ggctcgggca tcgtgcgcga aattggtccc ggtgtaactg atttcgcgcc cggcgatcac gtggtgatgt cattcatcgc ggtatgcggg caatgccgat ggtgcgccag cggcatggag tacctatgcg atgccggcat cggcaccctg acgcccggga tgcccaccga tggcacgttc cggcatcaca ccgccgacgg cacgccactg ggccatctgg cgaaggttgg cgcgttcgcc gagcacaccg tggtgtctcg ggattcactg gtcaagatcg atccggacct gccgctggcc cccagcgcgt tgctgtcctg cgcgatcccc accgggtacg ggtcggcagc gaatcgagcc ggcgtgcgcg gcggtgacac cgtcgtcgtc ctgggcgccg gcgggatcgg cacgggggcc attcagggag ctcggatcaa tggcgccgcg cagatcgtgg ccgttgatcc ggtggagttc aagcagaaat ccgcccacct cttcggcgcc acccacggcg ccggatcgat cagtgaggca atggctttgg ttcgtgaact gactcacggg gtgatggccg actcggtact ggtctcgccg tcgctgatca ccgccgatga cgtgcgcgac gccctacggc tcacccgcaa gggtggcacc tgcgtgctgg ccgggatgac ctcacaactg acccgctcgg tcaacatcga cctacaggaa ttcatcttga tgaacaagac cctggccggc accatcttcg gctcgtgcaa ccccagggcc gacatcgcgc gtcttgccgg cttgtatcag agcggacaac tgcaactcga cgatatgatc acccgccgct atcgcctcga tgacatcaac gccgcctaca ccgacctgct caacggtgac atcgtcagag gcataatcga tttcacgatc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa