MypaA.10611.a

AdhD

CENTER ID: MypaA.10611.a
ORGANISM: Mycobacterium paratuberculosis ATCC BAA-968 / K-10
ASSOCIATED DISEASE: Johne's disease
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.10611.a.A1.GE29638 Full length( MypaA.10611.a ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MypaA.10611.a.A1.PS00778 Full length( MypaA.10611.a ) 1 386
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127257
PATRIC ID: fig|262316.17.peg.3727
RefSeq: NP_962436.1
UniProt: Q73U66
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKCRAAVIRG VGRDWEIAEI ELDPPRSGEV LVRMAVAGIC HSDDHLFTGD VVPTPEVVAA SGQPAPDWFP LLGGHEGAGV VAEVGPNVTS VRPGDHVALS FIPACGSCRF CVNGQSYICD IGASLFQREM PTDGTCRRHL GAENLLAYGQ LGTFAEYAVL SERSVIKIDE AIPFHAASLV SCGVSTGWGS ATVSAGTEPG DTVVVIGAGG VGMNALQGAR AVGAKYVVAV DPVAAKRDSA KIFGATHTAA SARDAIPLVR EITAGLMADR VVVCPGVVHV DLIPLALALL RKGGVCVLTG ITPFTEPPVP LVLQEMTLSA KQLRGALYGG MNPRTSVPML LSLYQAGDLK LDELVTRHYR LDEINEAFAD LREGRNIRGV IDFASR
NT Sequence
ATGAAGTGTC GTGCCGCCGT GATCCGCGGG GTCGGCCGGG ACTGGGAGAT CGCCGAGATC GAGCTCGACC CGCCGCGCAG CGGTGAGGTG CTGGTGCGGA TGGCCGTCGC CGGGATCTGC CACTCCGACG ACCATCTGTT CACCGGTGAC GTGGTGCCGA CGCCCGAGGT GGTCGCCGCG AGCGGTCAGC CCGCGCCGGA CTGGTTCCCG CTGCTCGGCG GTCACGAGGG CGCCGGTGTC GTCGCGGAAG TCGGGCCGAA CGTGACGTCG GTGCGCCCGG GCGACCACGT GGCGCTGTCG TTCATCCCGG CGTGCGGCAG CTGCCGGTTC TGCGTGAACG GCCAGAGCTA CATCTGCGAC ATCGGCGCCA GCCTGTTTCA GCGCGAGATG CCCACCGACG GGACCTGCCG CCGCCATCTC GGTGCGGAAA ACCTGTTGGC CTACGGGCAA CTCGGCACCT TCGCCGAGTA CGCGGTGCTC TCCGAACGGT CCGTCATCAA GATCGACGAG GCGATTCCGT TCCATGCCGC CTCCCTGGTG TCGTGCGGAG TCAGCACCGG ATGGGGCTCG GCGACGGTGT CGGCGGGCAC CGAACCGGGC GACACCGTGG TGGTGATCGG CGCCGGCGGT GTCGGGATGA ACGCGTTGCA GGGCGCCCGC GCGGTGGGCG CGAAGTACGT CGTCGCGGTC GATCCCGTTG CGGCCAAACG GGATTCGGCC AAGATCTTCG GCGCCACGCA CACCGCCGCG TCCGCGCGGG ACGCCATACC GCTGGTCAGG GAGATCACCG CCGGCCTGAT GGCCGACCGG GTGGTGGTCT GTCCCGGCGT GGTGCACGTG GACTTGATCC CGTTGGCGCT GGCGCTGCTG CGCAAGGGCG GGGTCTGCGT GCTGACCGGC ATCACCCCGT TCACCGAGCC CCCGGTTCCG CTGGTGCTGC AGGAGATGAC GCTGTCGGCC AAACAACTGC GGGGCGCCCT CTACGGCGGG ATGAATCCGC GCACCAGCGT GCCGATGCTG TTGTCCCTGT ATCAGGCGGG CGATTTGAAA CTCGACGAGT TGGTCACCCG CCACTACCGG CTCGACGAGA TCAACGAGGC ATTCGCCGAC CTGCGCGAGG GCCGCAACAT CCGCGGCGTC ATCGACTTCG CCAGCCGG
Details for MypaA.10611.a.A1.GE29638
HARVESTED ON: 6/22/2010
SEQUENCED ON: 7/1/2010
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKCRAAVIRG VGRDWEIAEI ELDPPRSGEV LVRMAVAGIC HSDDHLFTGD VVPTPEVVAA SGQPAPDWFP LLGGHEGAGV VAEVGPNVTS VRPGDHVALS FIPACGSCRF CVNGQSYICD IGASLFQREM PTDGTCRRHL GAENLLAYGQ LGTFAEYAVL SERSVIKIDE AIPFHAASLV SCGVSTGWGS ATVSAGTEPG DTVVVIGAGG VGMNALQGAR AVGAKYVVAV DPVAAKRDSA KIFGATHTAA SARDAIPLVR EITAGLMADR VVVCPGVVHV DLIPLALALL RKGGVCVLTG ITPFTEPPVP LVLQEMTLSA KQLRGALYGG MNPRTSVPML LSLYQAGDLK LDELVTRHYR LDEINEAFAD LREGRNIRGV IDFAS
Validated NT Sequence
cggctggcga agtcgatgac gccgcggatg ttgcggccct cgcgcaggtc ggcgaatgcc tcgttgatct cgtcgagccg gtagtggcgg gtgaccaact cgtcgagttt caaatcgccc gcctgataca gggacaacag catcggcacg ctggtgcgcg gattcatccc gccgtagagg gcgccccgca gttgtttggc cgacagcgtc atctcctgca gcaccagcgg aaccgggggc tcggtgaacg gggtgatgcc ggtcagcacg cagaccccgc ccttgcgcag cagcgccagc gccaacggga tcaagtccac gtgcaccacg ccgggacaga ccaccacccg gtcggccatc aggccggcgg tgatctccct gaccagcggt atggcgtccc gcgcggacgc ggcggtgtgc gtggcgccga agatcttggc cgaatcccgt ttggccgcaa cgggatcgac cgcgacgacg tacttcgcgc ccaccgcgcg ggcgccctgc aacgcgttca tcccgacacc gccggcgccg atcaccacca cggtgtcgcc cggttcggtg cccgccgaca ccgtcgccga gccccatccg gtgctgactc cgcacgacac cagggaggcg gcatggaacg gaatcgcctc gtcgatcttg atgacggacc gttcggagag caccgcgtac tcggcgaagg tgccgagttg cccgtaggcc aacaggtttt ccgcaccgag atggcggcgg caggtcccgt cggtgggcat ctcgcgctga aacaggctgg cgccgatgtc gcagatgtag ctctggccgt tcacgcagaa ccggcagctg ccgcacgccg ggatgaacga cagcgccacg tggtcgcccg ggcgcaccga cgtcacgttc ggcccgactt ccgcgacgac accggcgccc tcgtgaccgc cgagcagcgg gaaccagtcc ggcgcgggct gaccgctcgc ggcgaccacc tcgggcgtcg gcaccacgtc accggtgaac agatggtcgt cggagtggca gatcccggcg acggccatcc gcaccagcac ctcaccgctg cgcggcgggt cgagctcgat ctcggcgatc tcccagtccc ggccgacccc gcggatcacg gcggcacgac acttcatcga accaggaccc tgggtctgag cttccagggt acc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKCRAAVIR GVGRDWEIAE IELDPPRSGE VLVRMAVAGI CHSDDHLFTG DVVPTPEVVA ASGQPAPDWF PLLGGHEGAG VVAEVGPNVT SVRPGDHVAL SFIPACGSCR FCVNGQSYIC DIGASLFQRE MPTDGTCRRH LGAENLLAYG QLGTFAEYAV LSERSVIKID EAIPFHAASL VSCGVSTGWG SATVSAGTEP GDTVVVIGAG GVGMNALQGA RAVGAKYVVA VDPVAAKRDS AKIFGATHTA ASARDAIPLV REITAGLMAD RVVVCPGVVH VDLIPLALAL LRKGGVCVLT GITPFTEPPV PLVLQEMTLS AKQLRGALYG GMNPRTSVPM LLSLYQAGDL KLDELVTRHY RLDEINEAFA DLREGRNIRG VIDFASR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagtgtcgt gccgccgtga tccgcggggt cggccgggac tgggagatcg ccgagatcga gctcgacccg ccgcgcagcg gtgaggtgct ggtgcggatg gccgtcgccg ggatctgcca ctccgacgac catctgttca ccggtgacgt ggtgccgacg cccgaggtgg tcgccgcgag cggtcagccc gcgccggact ggttcccgct gctcggcggt cacgagggcg ccggtgtcgt cgcggaagtc gggccgaacg tgacgtcggt gcgcccgggc gaccacgtgg cgctgtcgtt catcccggcg tgcggcagct gccggttctg cgtgaacggc cagagctaca tctgcgacat cggcgccagc ctgtttcagc gcgagatgcc caccgacggg acctgccgcc gccatctcgg tgcggaaaac ctgttggcct acgggcaact cggcaccttc gccgagtacg cggtgctctc cgaacggtcc gtcatcaaga tcgacgaggc gattccgttc catgccgcct ccctggtgtc gtgcggagtc agcaccggat ggggctcggc gacggtgtcg gcgggcaccg aaccgggcga caccgtggtg gtgatcggcg ccggcggtgt cgggatgaac gcgttgcagg gcgcccgcgc ggtgggcgcg aagtacgtcg tcgcggtcga tcccgttgcg gccaaacggg attcggccaa gatcttcggc gccacgcaca ccgccgcgtc cgcgcgggac gccataccgc tggtcaggga gatcaccgcc ggcctgatgg ccgaccgggt ggtggtctgt cccggcgtgg tgcacgtgga cttgatcccg ttggcgctgg cgctgctgcg caagggcggg gtctgcgtgc tgaccggcat caccccgttc accgagcccc cggttccgct ggtgctgcag gagatgacgc tgtcggccaa acaactgcgg ggcgccctct acggcgggat gaatccgcgc accagcgtgc cgatgctgtt gtccctgtat caggcgggcg atttgaaact cgacgagttg gtcacccgcc actaccggct cgacgagatc aacgaggcat tcgccgacct gcgcgagggc cgcaacatcc gcggcgtcat cgacttcgcc agccggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MypaA.10611.a.A1.PS00778
PURIFICATION DATe: 9/10/2010
CONCENTRATION: 14.76mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MKCRAAVIRG VGRDWEIAEI ELDPPRSGEV LVRMAVAGIC HSDDHLFTGD VVPTPEVVAA SGQPAPDWFP LLGGHEGAGV VAEVGPNVTS VRPGDHVALS FIPACGSCRF CVNGQSYICD IGASLFQREM PTDGTCRRHL GAENLLAYGQ LGTFAEYAVL SERSVIKIDE AIPFHAASLV SCGVSTGWGS ATVSAGTEPG DTVVVIGAGG VGMNALQGAR AVGAKYVVAV DPVAAKRDSA KIFGATHTAA SARDAIPLVR EITAGLMADR VVVCPGVVHV DLIPLALALL RKGGVCVLTG ITPFTEPPVP LVLQEMTLSA KQLRGALYGG MNPRTSVPML LSLYQAGDLK LDELVTRHYR LDEINEAFAD LREGRNIRGV IDFAS
Validated NT Sequence
cggctggcga agtcgatgac gccgcggatg ttgcggccct cgcgcaggtc ggcgaatgcc tcgttgatct cgtcgagccg gtagtggcgg gtgaccaact cgtcgagttt caaatcgccc gcctgataca gggacaacag catcggcacg ctggtgcgcg gattcatccc gccgtagagg gcgccccgca gttgtttggc cgacagcgtc atctcctgca gcaccagcgg aaccgggggc tcggtgaacg gggtgatgcc ggtcagcacg cagaccccgc ccttgcgcag cagcgccagc gccaacggga tcaagtccac gtgcaccacg ccgggacaga ccaccacccg gtcggccatc aggccggcgg tgatctccct gaccagcggt atggcgtccc gcgcggacgc ggcggtgtgc gtggcgccga agatcttggc cgaatcccgt ttggccgcaa cgggatcgac cgcgacgacg tacttcgcgc ccaccgcgcg ggcgccctgc aacgcgttca tcccgacacc gccggcgccg atcaccacca cggtgtcgcc cggttcggtg cccgccgaca ccgtcgccga gccccatccg gtgctgactc cgcacgacac cagggaggcg gcatggaacg gaatcgcctc gtcgatcttg atgacggacc gttcggagag caccgcgtac tcggcgaagg tgccgagttg cccgtaggcc aacaggtttt ccgcaccgag atggcggcgg caggtcccgt cggtgggcat ctcgcgctga aacaggctgg cgccgatgtc gcagatgtag ctctggccgt tcacgcagaa ccggcagctg ccgcacgccg ggatgaacga cagcgccacg tggtcgcccg ggcgcaccga cgtcacgttc ggcccgactt ccgcgacgac accggcgccc tcgtgaccgc cgagcagcgg gaaccagtcc ggcgcgggct gaccgctcgc ggcgaccacc tcgggcgtcg gcaccacgtc accggtgaac agatggtcgt cggagtggca gatcccggcg acggccatcc gcaccagcac ctcaccgctg cgcggcgggt cgagctcgat ctcggcgatc tcccagtccc ggccgacccc gcggatcacg gcggcacgac acttcatcga accaggaccc tgggtctgag cttccagggt acc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKCRAAVIR GVGRDWEIAE IELDPPRSGE VLVRMAVAGI CHSDDHLFTG DVVPTPEVVA ASGQPAPDWF PLLGGHEGAG VVAEVGPNVT SVRPGDHVAL SFIPACGSCR FCVNGQSYIC DIGASLFQRE MPTDGTCRRH LGAENLLAYG QLGTFAEYAV LSERSVIKID EAIPFHAASL VSCGVSTGWG SATVSAGTEP GDTVVVIGAG GVGMNALQGA RAVGAKYVVA VDPVAAKRDS AKIFGATHTA ASARDAIPLV REITAGLMAD RVVVCPGVVH VDLIPLALAL LRKGGVCVLT GITPFTEPPV PLVLQEMTLS AKQLRGALYG GMNPRTSVPM LLSLYQAGDL KLDELVTRHY RLDEINEAFA DLREGRNIRG VIDFASR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGTGTCGT GCCGCCGTGA TCCGCGGGGT CGGCCGGGAC TGGGAGATCG CCGAGATCGA GCTCGACCCG CCGCGCAGCG GTGAGGTGCT GGTGCGGATG GCCGTCGCCG GGATCTGCCA CTCCGACGAC CATCTGTTCA CCGGTGACGT GGTGCCGACG CCCGAGGTGG TCGCCGCGAG CGGTCAGCCC GCGCCGGACT GGTTCCCGCT GCTCGGCGGT CACGAGGGCG CCGGTGTCGT CGCGGAAGTC GGGCCGAACG TGACGTCGGT GCGCCCGGGC GACCACGTGG CGCTGTCGTT CATCCCGGCG TGCGGCAGCT GCCGGTTCTG CGTGAACGGC CAGAGCTACA TCTGCGACAT CGGCGCCAGC CTGTTTCAGC GCGAGATGCC CACCGACGGG ACCTGCCGCC GCCATCTCGG TGCGGAAAAC CTGTTGGCCT ACGGGCAACT CGGCACCTTC GCCGAGTACG CGGTGCTCTC CGAACGGTCC GTCATCAAGA TCGACGAGGC GATTCCGTTC CATGCCGCCT CCCTGGTGTC GTGCGGAGTC AGCACCGGAT GGGGCTCGGC GACGGTGTCG GCGGGCACCG AACCGGGCGA CACCGTGGTG GTGATCGGCG CCGGCGGTGT CGGGATGAAC GCGTTGCAGG GCGCCCGCGC GGTGGGCGCG AAGTACGTCG TCGCGGTCGA TCCCGTTGCG GCCAAACGGG ATTCGGCCAA GATCTTCGGC GCCACGCACA CCGCCGCGTC CGCGCGGGAC GCCATACCGC TGGTCAGGGA GATCACCGCC GGCCTGATGG CCGACCGGGT GGTGGTCTGT CCCGGCGTGG TGCACGTGGA CTTGATCCCG TTGGCGCTGG CGCTGCTGCG CAAGGGCGGG GTCTGCGTGC TGACCGGCAT CACCCCGTTC ACCGAGCCCC CGGTTCCGCT GGTGCTGCAG GAGATGACGC TGTCGGCCAA ACAACTGCGG GGCGCCCTCT ACGGCGGGAT GAATCCGCGC ACCAGCGTGC CGATGCTGTT GTCCCTGTAT CAGGCGGGCG ATTTGAAACT CGACGAGTTG GTCACCCGCC ACTACCGGCT CGACGAGATC AACGAGGCAT TCGCCGACCT GCGCGAGGGC CGCAACATCC GCGGCGTCAT CGACTTCGCC AGCCGGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA