MyulA.10611.a

Zinc-containing alcohol dehydrogenase NAD-dependent AdhB

CENTER ID: MyulA.10611.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.10611.a.A1.GE29964 Full length( MyulA.10611.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_127257
PATRIC ID: fig|362242.7.peg.628
RefSeq: YP_904644.1
UniProt: A0PLF4
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTKGALIWE FNQPWSVEDI EIGDPVKDEV KIQMEAAGMC RSDNHLVTGG IPMAGFPVLG GHEGAGVVTE VGPGVDDIAP GDHVVLSFIP SCGRCPTCQA GLRNLCDLGA GLLAGAAVSD GTFRIQARGQ NVYPMTLLGT FSPYMVVHRS SVVKIDPAIP FEVAALVGCG VTTGYGSAVR TADVRPGDDV AIVGLGGVGM AALQGAVTAG ARYIFAVEPV EWKRDQALKF GATHVYPDME AALMGIAEVT YGLMAKKVMI TVGELQGSDI DTYLAITSKG GTCVVTAIGS LLDTQVNLNL AMLTLMQKNL QGTIFGGGNP QYDIPHLLSM YKARNLNIDD MITRQYRLEQ INEGYQDMID GKNIRGVIRF TDADR
NT Sequence
GTGAAGACAA AAGGCGCTCT GATCTGGGAG TTCAACCAGC CGTGGTCGGT CGAGGACATC GAAATCGGCG ATCCGGTCAA AGACGAGGTC AAGATTCAGA TGGAAGCGGC GGGCATGTGC CGCTCCGACA ACCACCTGGT CACCGGCGGC ATTCCGATGG CGGGTTTCCC GGTCCTGGGC GGACATGAGG GCGCCGGTGT CGTGACCGAG GTCGGCCCCG GTGTGGACGA CATCGCCCCC GGCGACCACG TGGTGTTGTC CTTCATCCCG TCCTGCGGCA GATGCCCGAC CTGTCAGGCC GGCCTGCGCA ATCTCTGCGA CCTGGGGGCG GGGTTGCTGG CCGGCGCCGC GGTGTCCGAC GGCACCTTCC GCATCCAGGC CCGCGGCCAG AACGTCTACC CCATGACCCT GTTGGGCACC TTCTCGCCGT ACATGGTGGT ACACCGCAGC TCGGTGGTGA AGATCGACCC GGCCATTCCT TTCGAGGTCG CCGCCCTGGT GGGTTGCGGC GTCACCACCG GCTATGGCTC GGCAGTTCGT ACCGCCGACG TCCGCCCCGG CGACGACGTC GCCATCGTCG GTCTTGGCGG GGTCGGCATG GCGGCGCTGC AGGGCGCGGT GACCGCCGGC GCCCGCTACA TCTTCGCCGT CGAGCCGGTG GAGTGGAAAC GCGACCAGGC GCTCAAGTTC GGCGCCACCC ATGTCTATCC CGACATGGAG GCGGCGCTCA TGGGGATCGC CGAAGTCACC TACGGGCTGA TGGCCAAGAA AGTGATGATC ACCGTCGGCG AGTTGCAGGG CTCCGACATC GACACCTACT TGGCGATCAC CTCAAAGGGC GGTACCTGCG TGGTCACCGC GATCGGCAGC CTGCTGGACA CTCAGGTCAA CCTGAACCTG GCAATGCTGA CCCTGATGCA GAAGAACTTG CAGGGCACCA TCTTCGGCGG CGGTAACCCG CAATACGACA TTCCGCACCT GCTGTCGATG TACAAGGCGC GCAACCTCAA CATCGACGAC ATGATCACCC GCCAGTACCG GCTGGAACAG ATCAACGAGG GCTACCAGGA CATGATTGAC GGCAAGAACA TTCGCGGCGT CATCCGCTTC ACCGACGCCG ACCGG
Details for MyulA.10611.a.A1.GE29964
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVKTKGALIW EFNQPWSVED IEIGDPVKDE VKIQMEAAGM CRSDNHLVTG GIPMAGFPVL GGHEGAGVVT EVGPGVDDIA PGDHVVLSFI PSCGRCPTCQ AGLRNLCDLG AGLLAGAAVS DGTFRIQARG QNVYPMTLLG TFSPYMVVHR SSVVKIDPAI PFEVAALVGC GVTTGYGSAV RTADVRPGDD VAIVGLGGVG MAALQGAVTA GARYIFAVEP VEWKRDQALK FGATHVYPDM EAALMGIAEV TYGLMAKKVM ITVGELQGSD IDTYLAITSK GGTCVVTAIG SLLDTQVNLN LAMLTLMQKN LQGTIFGGGN PQYDIPHLLS MYKARNLNID DMITRQYRLE QINEGYQDMI DGKNIRGVIR FTDAD
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatggtgaa gacaaaaggc gctctgatct gggagttcaa ccagccgtgg tcggtcgagg acatcgaaat cggcgatccg gtcaaagacg aggtcaagat tcagatggaa gcggcgggca tgtgccgctc cgacaaccac ctggtcaccg gcggcattcc gatggcgggt ttcccggtcc tgggcggaca tgagggcgcc ggtgtcgtga ccgaggtcgg ccccggtgtg gacgacatcg cccccggcga ccacgtggtg ttgtccttca tcccgtcctg cggcagatgc ccgacctgtc aggccggcct gcgcaatctc tgcgacctgg gggcggggtt gctggccggc gccgcggtgt ccgacggcac cttccgcatc caggcccgcg gccagaacgt ctaccccatg accctgttgg gcaccttctc gccgtacatg gtggtacacc gcagctcggt ggtgaagatc gacccggcca ttcctttcga ggtcgccgcc ctggtgggtt gcggcgtcac caccggctat ggctcggcag ttcgtaccgc cgacgtccgc cccggcgacg acgtcgccat cgtcggtctt ggcggggtcg gcatggcggc gctgcagggc gcggtgaccg ccggcgcccg ctacatcttc gccgtcgagc cggtggagtg gaaacgcgac caggcgctca agttcggcgc cacccatgtc tatcccgaca tggaggcggc gctcatgggg atcgccgaag tcacctacgg gctgatggcc aagaaagtga tgatcaccgt cggcgagttg cagggctccg acatcgacac ctacttggcg atcacctcaa agggcggtac ctgcgtggtc accgcgatcg gcagcctgct ggacactcag gtcaacctga acctggcaat gctgaccctg atgcagaaga acttgcaggg caccatcttc ggcggcggta acccgcaata cgacattccg cacctgctgt cgatgtacaa ggcgcgcaac ctcaacatcg acgacatgat cacccgccag taccggctgg aacagatcaa cgagggctac caggacatga ttgacggcaa gaacattcgc ggcgtcatcc gcttcaccga cgccgact
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKTKGALI WEFNQPWSVE DIEIGDPVKD EVKIQMEAAG MCRSDNHLVT GGIPMAGFPV LGGHEGAGVV TEVGPGVDDI APGDHVVLSF IPSCGRCPTC QAGLRNLCDL GAGLLAGAAV SDGTFRIQAR GQNVYPMTLL GTFSPYMVVH RSSVVKIDPA IPFEVAALVG CGVTTGYGSA VRTADVRPGD DVAIVGLGGV GMAALQGAVT AGARYIFAVE PVEWKRDQAL KFGATHVYPD MEAALMGIAE VTYGLMAKKV MITVGELQGS DIDTYLAITS KGGTCVVTAI GSLLDTQVNL NLAMLTLMQK NLQGTIFGGG NPQYDIPHLL SMYKARNLNI DDMITRQYRL EQINEGYQDM IDGKNIRGVI RFTDAD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaagaca aaaggcgctc tgatctggga gttcaaccag ccgtggtcgg tcgaggacat cgaaatcggc gatccggtca aagacgaggt caagattcag atggaagcgg cgggcatgtg ccgctccgac aaccacctgg tcaccggcgg cattccgatg gcgggtttcc cggtcctggg cggacatgag ggcgccggtg tcgtgaccga ggtcggcccc ggtgtggacg acatcgcccc cggcgaccac gtggtgttgt ccttcatccc gtcctgcggc agatgcccga cctgtcaggc cggcctgcgc aatctctgcg acctgggggc ggggttgctg gccggcgccg cggtgtccga cggcaccttc cgcatccagg cccgcggcca gaacgtctac cccatgaccc tgttgggcac cttctcgccg tacatggtgg tacaccgcag ctcggtggtg aagatcgacc cggccattcc tttcgaggtc gccgccctgg tgggttgcgg cgtcaccacc ggctatggct cggcagttcg taccgccgac gtccgccccg gcgacgacgt cgccatcgtc ggtcttggcg gggtcggcat ggcggcgctg cagggcgcgg tgaccgccgg cgcccgctac atcttcgccg tcgagccggt ggagtggaaa cgcgaccagg cgctcaagtt cggcgccacc catgtctatc ccgacatgga ggcggcgctc atggggatcg ccgaagtcac ctacgggctg atggccaaga aagtgatgat caccgtcggc gagttgcagg gctccgacat cgacacctac ttggcgatca cctcaaaggg cggtacctgc gtggtcaccg cgatcggcag cctgctggac actcaggtca acctgaacct ggcaatgctg accctgatgc agaagaactt gcagggcacc atcttcggcg gcggtaaccc gcaatacgac attccgcacc tgctgtcgat gtacaaggcg cgcaacctca acatcgacga catgatcacc cgccagtacc ggctggaaca gatcaacgag ggctaccagg acatgattga cggcaagaac attcgcggcg tcatccgctt caccgacgcc gacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa