SSGCID: Target Page

MytuD.01026.a

4-aminobutyrate aminotransferase

CENTER ID:	MytuD.01026.a
ORGANISM:	Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE:	Tuberculosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.01026.a.A1.GE25925	Full length( MytuD.01026.a )		1	449

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MytuD.01026.a.A1.PS00203b	Full length( MytuD.01026.a )		1	449

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|83332.12.peg.2898
RefSeq:	NP_217105.1
TubercuList:	Rv2589
MTB Network Portal:	Rv2589
UniProt:	P9WQ79

Orthologues for Rv2589

Species	% Identity	% Coverage	Clones	Proteins	Structures
avium	85	100	1	0	0
paratuberculosis	85	100	0	0	0
leprae	84	100	1	0	0
marinum	84	100	1	1	1
ulcerans	83	100	1	0	0
smegmatis	78	99	0	1	1
smegmatis	69	100	0	1	1
abscessus	64	99	1	1	1
smegmatis	58	98	1	1	0
abscessus	54	98	1	0	0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASLQQSRRL VTEIPGPASQ ALTHRRAAAV SSGVGVTLPV FVARAGGGIV EDVDGNRLID LGSGIAVTTI GNSSPRVVDA VRTQVAEFTH TCFMVTPYEG YVAVAEQLNR ITPGSGPKRS VLFNSGAEAV ENAVKIARSY TGKPAVVAFD HAYHGRTNLT MALTAKSMPY KSGFGPFAPE IYRAPLSYPY RDGLLDKQLA TNGELAAARA IGVIDKQVGA NNLAALVIEP IQGEGGFIVP AEGFLPALLD WCRKNHVVFI ADEVQTGFAR TGAMFACEHE GPDGLEPDLI CTAKGIADGL PLSAVTGRAE IMNAPHVGGL GGTFGGNPVA CAAALATIAT IESDGLIERA RQIERLVTDR LTTLQAVDDR IGDVRGRGAM IAVELVKSGT TEPDAGLTER LATAAHAAGV IILTCGMFGN IIRLLPPLTI GDELLSEGLD IVCAILADL

NT Sequence

GTGGCCAGCC TGCAGCAGAG TCGGCGCCTG GTCACCGAAA TCCCCGGTCC CGCATCGCAG GCACTGACTC ACCGCCGGGC GGCGGCGGTG TCCAGCGGTG TTGGGGTCAC CCTGCCGGTG TTCGTAGCCC GCGCCGGCGG CGGCATCGTG GAAGACGTGG ACGGTAACCG GCTCATCGAC CTGGGTTCGG GCATCGCAGT GACGACGATC GGCAACTCGT CGCCACGCGT GGTGGATGCG GTGCGCACGC AGGTGGCCGA ATTTACCCAC ACCTGCTTCA TGGTGACGCC ATACGAGGGG TACGTGGCCG TCGCCGAGCA ACTCAACCGG ATTACCCCAG GTTCGGGCCC CAAGCGCTCG GTGTTGTTCA ATTCCGGCGC CGAGGCAGTC GAGAACGCCG TCAAGATCGC ACGCTCCTAC ACCGGCAAGC CCGCGGTGGT GGCGTTCGAC CACGCCTACC ACGGTCGCAC CAACCTAACG ATGGCGCTGA CCGCCAAGTC GATGCCCTAC AAGAGCGGCT TCGGTCCGTT CGCGCCGGAG ATCTACCGAG CGCCATTGTC TTACCCCTAT CGGGACGGCC TCCTCGATAA GCAACTGGCT ACCAATGGTG AGCTAGCCGC GGCCCGAGCC ATCGGCGTCA TCGACAAGCA GGTAGGCGCG AACAACCTGG CCGCCCTCGT CATCGAACCG ATCCAGGGCG AAGGCGGTTT CATCGTTCCG GCCGAAGGGT TCCTACCTGC CCTCCTCGAT TGGTGCCGCA AGAACCATGT GGTGTTCATC GCCGACGAGG TGCAAACCGG CTTTGCCCGT ACCGGGGCGA TGTTCGCCTG CGAGCACGAG GGCCCCGACG GTCTAGAGCC CGACCTGATC TGCACGGCCA AAGGCATCGC CGATGGATTG CCGCTGTCGG CGGTCACCGG CCGCGCCGAG ATCATGAACG CCCCGCACGT GGGCGGCCTG GGCGGCACGT TCGGCGGCAA CCCGGTGGCC TGTGCGGCCG CGCTGGCCAC CATCGCAACC ATCGAAAGCG ACGGGCTGAT CGAGCGGGCC CGCCAGATCG AACGCCTGGT GACCGACCGG TTGACGACGC TGCAGGCCGT CGACGACCGG ATCGGCGACG TGCGTGGTCG CGGCGCCATG ATCGCCGTAG AGCTGGTCAA ATCCGGAACC ACCGAGCCCG ACGCCGGGCT GACCGAGCGG CTGGCGACCG CGGCCCACGC CGCCGGCGTC ATCATTTTGA CCTGCGGCAT GTTCGGCAAC ATCATCCGGC TACTGCCGCC GCTGACCATC GGCGACGAGC TGCTGAGTGA GGGGCTGGAC ATCGTGTGCG CGATCTTGGC CGACCTC

Details for MytuD.01026.a.A1.GE25925

HARVESTED ON:	1/9/2009
SEQUENCED ON:	8/18/2010
EXPECTED MW:	49kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVASLQQSR RLVTEIPGPA SQALTHRRAA AVSSGVGVTL PVFVARAGGG IVEDVDGNRL IDLGSGIAVT TIGNSSPRVV DAVRTQVAEF THTCFMVTPY EGYVAVAEQL NRITPGSGPK RSVLFNSGAE AVENAVKIAR SYTGKPAVVA FDHAYHGRTN LTMALTAKSM PYKSGFXPFA PEIYRAPLSY PYRDGLLDKQ LATNGELAAA RAIGVIDKQV GANNLAALVI EPIQGEGGFI VPAEGFLPAL LDWCRKNHVV FIADEVQTGF ARTGAMFACE HEGPDGLEPD LICTAKGIAD GLPLSAVTGR AEIMNAPHVG GLGGTFGGNP VACAAALATI ATIESDGLIE RARQIERLVT DRLTTLQAVD DRIGDVRGRG AMIAVELVKS GTTEPDAGLT ERLATAAHAA GVIILTCGMF GNIIRLLPPL TIGDELLSEG LDIVCAILA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg ccagcctgca gcagagtcgg cgcctggtca ccgaaatccc cggtcccgca tcgcaggcac tgactcaccg ccgggcggcg gcggtgtcca gcggtgttgg ggtcaccctg ccggtgttcg tagcccgcgc cggcggcggc atcgtggaag acgtggacgg taaccggctc atcgacctgg gttcgggcat cgcagtgacg acgatcggca actcgtcgcc acgcgtggtg gatgcggtgc gcacgcaggt ggccgaattt acccacacct gcttcatggt gacgccatac gaggggtacg tggccgtcgc cgagcaactc aaccggatta ccccaggttc gggccccaag cgctcggtgt tgttcaattc cggcgccgag gcagtcgaga acgccgtcaa gatcgcacgc tcctacaccg gcaagcccgc ggtggtggcg ttcgaccacg cctaccacgg tcgcaccaac ctaacgatgg cgctgaccgc caagtcgatg ccctacaaga gcggcttcng tccgttcgcg ccggagatct accgagcgcc attgtcttac ccctatcggg acggcctcct cgataagcaa ctggctacca atggtgagct agccgcggcc cgagccatcg gcgtcatcga caagcaggta ggcgcgaaca acctggccgc cctcgtcatc gaaccgatcc agggcgaagg cggtttcatc gttccggccg aagggttcct acctgccctc ctcgattggt gccgcaagaa ccatgtggtg ttcatcgccg acgaggtgca aaccggcttt gcccgtaccg gggcgatgtt cgcctgcgag cacgagggcc ccgacggtct agagcccgac ctgatctgca cggccaaagg catcgccgat ggattgccgc tgtcggcggt caccggccgc gccgagatca tgaacgcccc gcacgtgggc ggcctgggcg gcacgttcgg cggcaacccg gtggcctgtg cggccgcgct ggccaccatc gcaaccatcg aaagcgacgg gctgatcgag cgggcccgcc agatcgaacg cctggtgacc gaccggttga cgacgctgca ggccgtcgac gaccggatcg gcgacgtgcg tggtcgcggc gccatgatcg ccgtagagct ggtcaaatcc ggaaccaccg agcccgacgc cgggctgacc gagcggctgg cgaccgcggc ccacgccgcc ggcgtcatca ttttgacctg cggcatgttc ggcaacatca tccggctact gccgccgctg accatcggcg acgagctgct gagtgagggg ctggacatcg tgtgcgcgat cttggccg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVASLQQSR RLVTEIPGPA SQALTHRRAA AVSSGVGVTL PVFVARAGGG IVEDVDGNRL IDLGSGIAVT TIGNSSPRVV DAVRTQVAEF THTCFMVTPY EGYVAVAEQL NRITPGSGPK RSVLFNSGAE AVENAVKIAR SYTGKPAVVA FDHAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPLSY PYRDGLLDKQ LATNGELAAA RAIGVIDKQV GANNLAALVI EPIQGEGGFI VPAEGFLPAL LDWCRKNHVV FIADEVQTGF ARTGAMFACE HEGPDGLEPD LICTAKGIAD GLPLSAVTGR AEIMNAPHVG GLGGTFGGNP VACAAALATI ATIESDGLIE RARQIERLVT DRLTTLQAVD DRIGDVRGRG AMIAVELVKS GTTEPDAGLT ERLATAAHAA GVIILTCGMF GNIIRLLPPL TIGDELLSEG LDIVCAILAD L

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccagc ctgcagcaga gtcggcgcct ggtcaccgaa atccccggtc ccgcatcgca ggcactgact caccgccggg cggcggcggt gtccagcggt gttggggtca ccctgccggt gttcgtagcc cgcgccggcg gcggcatcgt ggaagacgtg gacggtaacc ggctcatcga cctgggttcg ggcatcgcag tgacgacgat cggcaactcg tcgccacgcg tggtggatgc ggtgcgcacg caggtggccg aatttaccca cacctgcttc atggtgacgc catacgaggg gtacgtggcc gtcgccgagc aactcaaccg gattacccca ggttcgggcc ccaagcgctc ggtgttgttc aattccggcg ccgaggcagt cgagaacgcc gtcaagatcg cacgctccta caccggcaag cccgcggtgg tggcgttcga ccacgcctac cacggtcgca ccaacctaac gatggcgctg accgccaagt cgatgcccta caagagcggc ttcggtccgt tcgcgccgga gatctaccga gcgccattgt cttaccccta tcgggacggc ctcctcgata agcaactggc taccaatggt gagctagccg cggcccgagc catcggcgtc atcgacaagc aggtaggcgc gaacaacctg gccgccctcg tcatcgaacc gatccagggc gaaggcggtt tcatcgttcc ggccgaaggg ttcctacctg ccctcctcga ttggtgccgc aagaaccatg tggtgttcat cgccgacgag gtgcaaaccg gctttgcccg taccggggcg atgttcgcct gcgagcacga gggccccgac ggtctagagc ccgacctgat ctgcacggcc aaaggcatcg ccgatggatt gccgctgtcg gcggtcaccg gccgcgccga gatcatgaac gccccgcacg tgggcggcct gggcggcacg ttcggcggca acccggtggc ctgtgcggcc gcgctggcca ccatcgcaac catcgaaagc gacgggctga tcgagcgggc ccgccagatc gaacgcctgg tgaccgaccg gttgacgacg ctgcaggccg tcgacgaccg gatcggcgac gtgcgtggtc gcggcgccat gatcgccgta gagctggtca aatccggaac caccgagccc gacgccgggc tgaccgagcg gctggcgacc gcggcccacg ccgccggcgt catcattttg acctgcggca tgttcggcaa catcatccgg ctactgccgc cgctgaccat cggcgacgag ctgctgagtg aggggctgga catcgtgtgc gcgatcttgg ccgacctcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MytuD.01026.a.A1.PS00203b

PURIFICATION DATe:	4/9/2009
CONCENTRATION:	23.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVASLQQSR RLVTEIPGPA SQALTHRRAA AVSSGVGVTL PVFVARAGGG IVEDVDGNRL IDLGSGIAVT TIGNSSPRVV DAVRTQVAEF THTCFMVTPY EGYVAVAEQL NRITPGSGPK RSVLFNSGAE AVENAVKIAR SYTGKPAVVA FDHAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPLSY PYRDGLLDKQ LATNGELAAA RAIGVIDKQV GANNLAALVI EPIQGEGGFI VPAEGFLPAL LDWCRKNHVV FIADEVQTGF ARTGAMFACE HEGPDGLEPD LICTAKGIAD GLPLSAVTGR AEIMNAPHVG GLGGTFGGNP VACAAALATI ATIESDGLIE RARQIERLVT DRLTTLQAVD DRIGDVRGRG AMIAVELVKS GTTEPDAGLT ERLATAAHAA GVIILTCGMF GNIIRLLPPL TIGDELLSEG LDIVCAILAD L

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCCAGC CTGCAGCAGA GTCGGCGCCT GGTCACCGAA ATCCCCGGTC CCGCATCGCA GGCACTGACT CACCGCCGGG CGGCGGCGGT GTCCAGCGGT GTTGGGGTCA CCCTGCCGGT GTTCGTAGCC CGCGCCGGCG GCGGCATCGT GGAAGACGTG GACGGTAACC GGCTCATCGA CCTGGGTTCG GGCATCGCAG TGACGACGAT CGGCAACTCG TCGCCACGCG TGGTGGATGC GGTGCGCACG CAGGTGGCCG AATTTACCCA CACCTGCTTC ATGGTGACGC CATACGAGGG GTACGTGGCC GTCGCCGAGC AACTCAACCG GATTACCCCA GGTTCGGGCC CCAAGCGCTC GGTGTTGTTC AATTCCGGCG CCGAGGCAGT CGAGAACGCC GTCAAGATCG CACGCTCCTA CACCGGCAAG CCCGCGGTGG TGGCGTTCGA CCACGCCTAC CACGGTCGCA CCAACCTAAC GATGGCGCTG ACCGCCAAGT CGATGCCCTA CAAGAGCGGC TTCGGTCCGT TCGCGCCGGA GATCTACCGA GCGCCATTGT CTTACCCCTA TCGGGACGGC CTCCTCGATA AGCAACTGGC TACCAATGGT GAGCTAGCCG CGGCCCGAGC CATCGGCGTC ATCGACAAGC AGGTAGGCGC GAACAACCTG GCCGCCCTCG TCATCGAACC GATCCAGGGC GAAGGCGGTT TCATCGTTCC GGCCGAAGGG TTCCTACCTG CCCTCCTCGA TTGGTGCCGC AAGAACCATG TGGTGTTCAT CGCCGACGAG GTGCAAACCG GCTTTGCCCG TACCGGGGCG ATGTTCGCCT GCGAGCACGA GGGCCCCGAC GGTCTAGAGC CCGACCTGAT CTGCACGGCC AAAGGCATCG CCGATGGATT GCCGCTGTCG GCGGTCACCG GCCGCGCCGA GATCATGAAC GCCCCGCACG TGGGCGGCCT GGGCGGCACG TTCGGCGGCA ACCCGGTGGC CTGTGCGGCC GCGCTGGCCA CCATCGCAAC CATCGAAAGC GACGGGCTGA TCGAGCGGGC CCGCCAGATC GAACGCCTGG TGACCGACCG GTTGACGACG CTGCAGGCCG TCGACGACCG GATCGGCGAC GTGCGTGGTC GCGGCGCCAT GATCGCCGTA GAGCTGGTCA AATCCGGAAC CACCGAGCCC GACGCCGGGC TGACCGAGCG GCTGGCGACC GCGGCCCACG CCGCCGGCGT CATCATTTTG ACCTGCGGCA TGTTCGGCAA CATCATCCGG CTACTGCCGC CGCTGACCAT CGGCGACGAG CTGCTGAGTG AGGGGCTGGA CATCGTGTGC GCGATCTTGG CCGACCTCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA