SSGCID: Target Page

MysmA.01026.a

4-aminobutyrate transaminase

CENTER ID:	MysmA.01026.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01026.a.A1.GE28859	Full length( MysmA.01026.a )		1	450

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01026.a.A1.PW28764	Full length( MysmA.01026.a )		1	450

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.6508
RefSeq:	YP_890893.1
UniProt:	A0R6V5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTATLTGGPD LQQARRILTE IPGPRSRDLA ERRNAALPAG LASGAGVYIA AAGGGVVVDV DGNSFIDLGS GIAVTTVGNA APAVVSRAGS QLAQYTHTCF LNVPYEPYIE VAERLNALTP GDHQKRTALF STGAEAVENA VKYARAYTGR DAVVVFDHAF HGRSLLTMTM TAKNQPYKHG FGPFAPEVYR APMAYPYRWP SGPENCAAEA FDLFTTLIDA QIGADQVACV VVEPIQGEGG FIVPAEGFLQ KVADFCRERG ILVVADEVQT GIARTGAWFA CEHEDLVPDL ITTAKGLGGG LPLAAVTGRA EIMDAAHAGG IGGTYAGNPV ACAAALGVFD EIEQNGLIER ARAIGEVIVA ELRSIAATTG LIGEIRGRGA MIAVELIKPG TREPNKEAVA ALAKHCHDNG VLTLTAGTFG NVLRFLPPLT ITDELLVDAF GVIRDGFAAL

NT Sequence

ATGACAGCCA CGTTGACGGG CGGACCCGAT CTGCAGCAGG CCCGCCGGAT CCTGACTGAA ATCCCCGGCC CACGGTCCCG TGACCTGGCC GAACGCCGCA ACGCCGCGCT TCCGGCCGGC CTGGCGAGCG GGGCGGGCGT CTACATCGCC GCCGCAGGCG GCGGCGTGGT CGTGGACGTC GACGGCAATT CGTTCATCGA TCTGGGCAGC GGCATCGCGG TCACCACGGT CGGCAACGCC GCACCTGCGG TCGTCTCGCG CGCGGGCAGC CAACTCGCGC AGTACACGCA CACGTGCTTC CTGAACGTGC CCTACGAGCC GTACATCGAG GTCGCCGAGC GTCTCAACGC CCTGACCCCG GGCGATCACC AGAAGCGCAC CGCGCTGTTC AGCACGGGCG CCGAGGCCGT GGAGAACGCC GTCAAGTACG CACGCGCCTA CACCGGTCGC GACGCGGTCG TGGTGTTCGA CCACGCCTTC CACGGCCGGT CGCTGCTCAC CATGACCATG ACGGCCAAGA ACCAGCCCTA CAAGCACGGG TTCGGTCCGT TCGCCCCCGA GGTCTACCGC GCGCCCATGG CCTATCCCTA CCGGTGGCCG TCGGGGCCCG AAAACTGCGC GGCCGAGGCG TTCGACCTGT TCACCACCCT GATCGACGCG CAGATCGGCG CCGATCAGGT GGCCTGCGTC GTGGTCGAAC CCATCCAGGG CGAGGGCGGC TTCATCGTTC CCGCCGAGGG GTTCCTGCAG AAGGTCGCCG ACTTCTGCCG TGAGCGCGGC ATCCTGGTGG TGGCCGACGA GGTGCAGACC GGCATCGCGC GCACCGGCGC GTGGTTCGCG TGCGAGCACG AGGACCTGGT CCCCGACCTC ATCACCACCG CCAAGGGCCT GGGCGGTGGC CTGCCGCTGG CGGCGGTCAC CGGCCGCGCC GAGATCATGG ACGCCGCGCA CGCGGGCGGC ATCGGCGGCA CCTACGCGGG CAACCCCGTC GCGTGCGCCG CAGCACTCGG CGTGTTCGAC GAGATCGAGC AGAACGGCCT GATCGAGCGC GCCCGCGCCA TCGGCGAGGT GATCGTGGCA GAACTGCGCA GCATCGCCGC CACCACCGGC CTCATCGGCG AGATCCGCGG CCGCGGCGCC ATGATCGCCG TCGAGCTGAT CAAGCCCGGC ACGCGCGAAC CCAACAAGGA AGCCGTCGCC GCGCTGGCCA AGCACTGCCA CGACAACGGT GTACTGACCT TGACGGCAGG CACTTTCGGC AATGTCCTGC GTTTCCTGCC GCCGCTGACC ATCACCGACG AACTGCTCGT CGACGCCTTC GGCGTCATCC GCGACGGCTT CGCCGCCCTC

Details for MysmA.01026.a.A1.GE28859

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MGTLEAQTQG PGSMTATLTG GPDLQQARRI LTEIPGPRSR DLAERRNAAL PAGLASGAGV YIAAAGGGVV VDVDGNSFID LGSGIAVTTV GNAAPAVVSR AGSQLAQYTH TCFLNVPYEP YIEVAERLNA LTPGDHQKRT ALFSTGAEAV ENAVKYARAY TGRDAVVVFD HAFHGRSLLT MTMTAKNQPY KHGFGPFAPE VYRAPMAYPY RWPSGPENCA AEAFDLFTTL IDAQIGADQV ACVVVEPIQG EGGFIVPAEG FLQKVADFCR ERGILVVADE VQTGIARTGA WFACEHEDLV PDLITTAKGL GGGLPLAAVT GRAEIMDAAH AGGIGGTYAG NPVACAAALG VFDEIEQNGL IERARAIGEV IVAELRSIAA TTGLIGEIRG RGAMIAVELI KPGTREPNKE AVAALAKHCH DNGVLTLTAG TFGNVLRFLP PLTITDELLV DAFGVIRDGF AAL

Validated NT Sequence

ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacagcc acgttgacgg gcggacccga tctgcagcag gcccgccgga tcctgactga aatccccggc ccacggtccc gtgacctggc cgaacgccgc aacgccgcgc ttccggccgg cctggcgagc ggggcgggcg tctacatcgc cgccgcaggc ggcggcgtgg tcgtggacgt cgacggcaat tcgttcatcg atctgggcag cggcatcgcg gtcaccacgg tcggcaacgc cgcacctgcg gtcgtctcgc gcgcgggcag ccaactcgcg cagtacacgc acacgtgctt cctgaacgtg ccctacgagc cgtacatcga ggtcgccgag cgtctcaacg ccctgacccc gggcgatcac cagaagcgca ccgcgctgtt cagcacgggc gccgaggccg tggagaacgc cgtcaagtac gcacgcgcct acaccggtcg cgacgcggtc gtggtgttcg accacgcctt ccacggccgg tcgctgctca ccatgaccat gacggccaag aaccagccct acaagcacgg gttcggtccg ttcgcccccg aggtctaccg cgcgcccatg gcctatccct accggtggcc gtcggggccc gaaaactgcg cggccgaggc gttcgacctg ttcaccaccc tgatcgacgc gcagatcggc gccgatcagg tggcctgcgt cgtggtcgaa cccatccagg gcgagggcgg cttcatcgtt cccgccgagg ggttcctgca gaaggtcgcc gacttctgcc gtgagcgcgg catcctggtg gtggccgacg aggtgcagac cggcatcgcg cgcaccggcg cgtggttcgc gtgcgagcac gaggacctgg tccccgacct catcaccacc gccaagggcc tgggcggtgg cctgccgctg gcggcggtca ccggccgcgc cgagatcatg gacgccgcgc acgcgggcgg catcggcggc acctacgcgg gcaaccccgt cgcgtgcgcc gcagcactcg gcgtgttcga cgagatcgag cagaacggcc tgatcgagcg cgcccgcgcc atcggcgagg tgatcgtggc agaactgcgc agcatcgccg ccaccaccgg cctcatcggc gagatccgcg gccgcggcgc catgatcgcc gtcgagctga tcaagcccgg cacgcgcgaa cccaacaagg aagccgtcgc cgcgctggcc aagcactgcc acgacaacgg tgtactgacc ttgacggcag gcactttcgg caatgtcctg cgtttcctgc cgccgctgac catcaccgac gaactgctcg tcgacgcctt cggcgtcatc cgcgacggct tcgccgccct ctaat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTATLTGGP DLQQARRILT EIPGPRSRDL AERRNAALPA GLASGAGVYI AAAGGGVVVD VDGNSFIDLG SGIAVTTVGN AAPAVVSRAG SQLAQYTHTC FLNVPYEPYI EVAERLNALT PGDHQKRTAL FSTGAEAVEN AVKYARAYTG RDAVVVFDHA FHGRSLLTMT MTAKNQPYKH GFGPFAPEVY RAPMAYPYRW PSGPENCAAE AFDLFTTLID AQIGADQVAC VVVEPIQGEG GFIVPAEGFL QKVADFCRER GILVVADEVQ TGIARTGAWF ACEHEDLVPD LITTAKGLGG GLPLAAVTGR AEIMDAAHAG GIGGTYAGNP VACAAALGVF DEIEQNGLIE RARAIGEVIV AELRSIAATT GLIGEIRGRG AMIAVELIKP GTREPNKEAV AALAKHCHDN GVLTLTAGTF GNVLRFLPPL TITDELLVDA FGVIRDGFAA L

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagccacg ttgacgggcg gacccgatct gcagcaggcc cgccggatcc tgactgaaat ccccggccca cggtcccgtg acctggccga acgccgcaac gccgcgcttc cggccggcct ggcgagcggg gcgggcgtct acatcgccgc cgcaggcggc ggcgtggtcg tggacgtcga cggcaattcg ttcatcgatc tgggcagcgg catcgcggtc accacggtcg gcaacgccgc acctgcggtc gtctcgcgcg cgggcagcca actcgcgcag tacacgcaca cgtgcttcct gaacgtgccc tacgagccgt acatcgaggt cgccgagcgt ctcaacgccc tgaccccggg cgatcaccag aagcgcaccg cgctgttcag cacgggcgcc gaggccgtgg agaacgccgt caagtacgca cgcgcctaca ccggtcgcga cgcggtcgtg gtgttcgacc acgccttcca cggccggtcg ctgctcacca tgaccatgac ggccaagaac cagccctaca agcacgggtt cggtccgttc gcccccgagg tctaccgcgc gcccatggcc tatccctacc ggtggccgtc ggggcccgaa aactgcgcgg ccgaggcgtt cgacctgttc accaccctga tcgacgcgca gatcggcgcc gatcaggtgg cctgcgtcgt ggtcgaaccc atccagggcg agggcggctt catcgttccc gccgaggggt tcctgcagaa ggtcgccgac ttctgccgtg agcgcggcat cctggtggtg gccgacgagg tgcagaccgg catcgcgcgc accggcgcgt ggttcgcgtg cgagcacgag gacctggtcc ccgacctcat caccaccgcc aagggcctgg gcggtggcct gccgctggcg gcggtcaccg gccgcgccga gatcatggac gccgcgcacg cgggcggcat cggcggcacc tacgcgggca accccgtcgc gtgcgccgca gcactcggcg tgttcgacga gatcgagcag aacggcctga tcgagcgcgc ccgcgccatc ggcgaggtga tcgtggcaga actgcgcagc atcgccgcca ccaccggcct catcggcgag atccgcggcc gcggcgccat gatcgccgtc gagctgatca agcccggcac gcgcgaaccc aacaaggaag ccgtcgccgc gctggccaag cactgccacg acaacggtgt actgaccttg acggcaggca ctttcggcaa tgtcctgcgt ttcctgccgc cgctgaccat caccgacgaa ctgctcgtcg acgccttcgg cgtcatccgc gacggcttcg ccgccctcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for MysmA.01026.a.A1.PW28764

PURIFICATION DATe:	5/24/2010
CONCENTRATION:	23.69mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	16
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGCCACG TTGACGGGCG GACCCGATCT GCAGCAGGCC CGCCGGATCC TGACTGAAAT CCCCGGCCCA CGGTCCCGTG ACCTGGCCGA ACGCCGCAAC GCCGCGCTTC CGGCCGGCCT GGCGAGCGGG GCGGGCGTCT ACATCGCCGC CGCAGGCGGC GGCGTGGTCG TGGACGTCGA CGGCAATTCG TTCATCGATC TGGGCAGCGG CATCGCGGTC ACCACGGTCG GCAACGCCGC ACCTGCGGTC GTCTCGCGCG CGGGCAGCCA ACTCGCGCAG TACACGCACA CGTGCTTCCT GAACGTGCCC TACGAGCCGT ACATCGAGGT CGCCGAGCGT CTCAACGCCC TGACCCCGGG CGATCACCAG AAGCGCACCG CGCTGTTCAG CACGGGCGCC GAGGCCGTGG AGAACGCCGT CAAGTACGCA CGCGCCTACA CCGGTCGCGA CGCGGTCGTG GTGTTCGACC ACGCCTTCCA CGGCCGGTCG CTGCTCACCA TGACCATGAC GGCCAAGAAC CAGCCCTACA AGCACGGGTT CGGTCCGTTC GCCCCCGAGG TCTACCGCGC GCCCATGGCC TATCCCTACC GGTGGCCGTC GGGGCCCGAA AACTGCGCGG CCGAGGCGTT CGACCTGTTC ACCACCCTGA TCGACGCGCA GATCGGCGCC GATCAGGTGG CCTGCGTCGT GGTCGAACCC ATCCAGGGCG AGGGCGGCTT CATCGTTCCC GCCGAGGGGT TCCTGCAGAA GGTCGCCGAC TTCTGCCGTG AGCGCGGCAT CCTGGTGGTG GCCGACGAGG TGCAGACCGG CATCGCGCGC ACCGGCGCGT GGTTCGCGTG CGAGCACGAG GACCTGGTCC CCGACCTCAT CACCACCGCC AAGGGCCTGG GCGGTGGCCT GCCGCTGGCG GCGGTCACCG GCCGCGCCGA GATCATGGAC GCCGCGCACG CGGGCGGCAT CGGCGGCACC TACGCGGGCA ACCCCGTCGC GTGCGCCGCA GCACTCGGCG TGTTCGACGA GATCGAGCAG AACGGCCTGA TCGAGCGCGC CCGCGCCATC GGCGAGGTGA TCGTGGCAGA ACTGCGCAGC ATCGCCGCCA CCACCGGCCT CATCGGCGAG ATCCGCGGCC GCGGCGCCAT GATCGCCGTC GAGCTGATCA AGCCCGGCAC GCGCGAACCC AACAAGGAAG CCGTCGCCGC GCTGGCCAAG CACTGCCACG ACAACGGTGT ACTGACCTTG ACGGCAGGCA CTTTCGGCAA TGTCCTGCGT TTCCTGCCGC CGCTGACCAT CACCGACGAA CTGCTCGTCG ACGCCTTCGG CGTCATCCGC GACGGCTTCG CCGCCCTCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA