MymaA.01026.b
4-aminobutyrate aminotransferase GabT
| CENTER ID: | MymaA.01026.b |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01026.b.A1.GE30096 | Full length( MymaA.01026.b ) | 1 | 446 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.01026.b.A1.PW29968 | Full length( MymaA.01026.b ) | 1 | 446 |
Structures
3R4T
DEPOSITED: 3/17/2011
DETERMINATION: XRay
CLONE: MymaA.01026.b.A1.GE30096
PROTEIN: MymaA.01026.b.A1.PW29968
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216594.6.peg.2262 |
| RefSeq: | YP_001850423.1 |
| UniProt: | B2HN70 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASLDQSRLL VTEIPGPASL ELNKRRAAAV SGGVGVTLPV FVVRAGGGIV EDADGNRLID LGSGIAVTTI GNSAPRVVDA VRDQVEQFTH TCFMVTPYEQ YVAVAEQLNR ITPGSGEKRT VLFNSGAEAV ENSIKVARAH TRKQAVVAFD YAYHGRTNLT MALTAKSMPY KSGFGPFAPE IYRAPVSYPY RDNLLDKDIA TDGELAAERA INLIDKQIGA ANLAAVIIEP IAGEGGFIVP ADGFLPALQR WCRDNDVVFI ADEVQTGFAR TGAMFACDHE NVEPDLIVTA KGIADGFPLS AVTGRAEIMD APHTSGLGGT FGGNPVACAA ALATIETIER DGMVERARQI ERLVMDRLLR LQAADDRLGD VRGRGAMIAM ELVKSGTAEP DAALTQKLAA AAHAAGVIVL TCGMFGNVIR LLPPLTISDE LLSEGLDILC QILADL
NT Sequence
GTGGCCAGCC TCGATCAAAG TCGTCTCTTG GTCACCGAAA TCCCCGGCCC CGCGTCGTTG GAGCTCAACA AGCGCCGGGC CGCGGCGGTG TCGGGTGGCG TCGGGGTCAC CCTGCCGGTG TTCGTGGTTC GCGCGGGTGG CGGAATCGTG GAGGACGCCG ACGGCAACCG GCTGATCGAC CTCGGCTCCG GCATCGCCGT CACGACTATC GGCAATTCGG CGCCCCGCGT GGTGGACGCG GTACGGGACC AGGTGGAGCA GTTCACCCAC ACCTGCTTCA TGGTGACCCC CTACGAGCAA TACGTGGCCG TCGCCGAACA GCTCAATCGG ATCACCCCGG GCTCCGGCGA GAAGCGGACG GTGCTGTTCA ACTCCGGCGC CGAGGCGGTG GAGAACTCCA TCAAGGTCGC CCGCGCGCAC ACCCGTAAGC AGGCCGTGGT CGCGTTCGAC TACGCCTACC ACGGCCGCAC CAACCTGACG ATGGCGCTGA CCGCCAAGTC CATGCCGTAC AAGAGCGGCT TCGGCCCCTT TGCGCCGGAG ATCTACCGCG CACCGGTGTC CTACCCCTAC CGGGACAACC TGCTCGACAA AGACATCGCC ACCGACGGTG AGCTTGCCGC CGAACGCGCC ATCAACCTCA TCGACAAGCA GATCGGCGCC GCCAACCTGG CCGCGGTCAT CATCGAACCG ATCGCGGGCG AGGGCGGCTT CATCGTTCCG GCCGACGGTT TCCTGCCCGC GCTGCAGCGG TGGTGCCGCG ACAACGACGT GGTGTTCATC GCCGACGAGG TGCAGACCGG ATTTGCCCGC ACCGGCGCCA TGTTCGCGTG CGACCACGAG AACGTCGAGC CCGACCTGAT CGTCACCGCC AAAGGCATCG CCGACGGATT CCCCTTGTCG GCGGTCACCG GCCGGGCCGA GATCATGGAC GCCCCGCACA CCAGCGGCTT GGGCGGCACC TTCGGCGGCA ACCCGGTCGC GTGTGCGGCG GCCCTGGCCA CCATCGAAAC GATCGAACGC GACGGCATGG TCGAACGGGC CCGCCAGATC GAACGACTGG TGATGGACCG ATTACTGCGC CTGCAAGCCG CCGATGACCG TCTCGGCGAT GTGCGCGGCC GCGGCGCCAT GATCGCCATG GAGCTGGTGA AATCGGGCAC TGCCGAACCC GACGCTGCGC TCACCCAAAA GCTGGCCGCC GCCGCCCATG CCGCCGGCGT AATTGTCTTG ACGTGCGGAA TGTTCGGCAA CGTGATCCGG CTGCTGCCAC CACTGACGAT CAGCGACGAG CTGCTGTCCG AAGGCCTCGA CATCCTGTGC CAGATCCTGG CCGACCTC
Details for MymaA.01026.b.A1.GE30096
| HARVESTED ON: | 8/10/2010 |
| SEQUENCED ON: | 8/16/2010 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 86 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVASLDQSR LLVTEIPGPA SLELNKRRAA AVSGGVGVTL PVFVVRAGGG IVEDADGNRL IDLGSGIAVT TIGNSAPRVV DAVRDQVEQF THTCFMVTPY EQYVAVAEQL NRITPGSGEK RTVLFNSGAE AVENSIKVAR AHTRKQAVVA FDYAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPVSY PYRDNLLDKX IATDGELAAE RAINLIDKQI GAANLAAVII EPIAGEGGFI VPADGFLPAL QRWCRDNDVV FIADEVQTGF ARTGAMFACD HENVEPDLIV TAKGIADGFP LSAVTGRAEI MDAPHTSGLG GTFGGNPVAC AAALATIETI ERDGMVERAR QIERLVMDRL LRLQAADDRL GDVRGRGAMI AMELVK
Validated NT Sequence
atttcaccag ctccatggcg atcatggcgc cgcggccgcg cacatcgccg agacggtcat cggcggcttg caggcgcagt aatcggtcca tcaccagtcg ttcgatctgg cgggcccgtt cgaccatgcc gtcgcgttcg atcgtttcga tggtggccag ggccgccgca cacgcgaccg ggttgccgcc gaaggtgccg cccaagccgc tggtgtgcgg ggcgtccatg atctcggccc ggccggtgac cgccgacaag gggaatccgt cggcgatgcc tttggcggtg acgatcaggt cgggctcgac gttctcgtgg tcgcacgcga acatggcgcc ggtgcgggca aatccggtct gcacctcgtc ggcgatgaac accacgtcgt tgtcgcggca ccaccgctgc agcgcgggca ggaaaccgtc ggccggaacg atgaagccgc cctcgcccgc gatcggttcg atgatgaccg cggccaggtt ggcggcgccg atctgcttgt cgatgaggtt gatggcgcgt tcggcggcaa gctcaccgtc ggtggcgatg tntttgtcga gcaggttgtc ccggtagggg taggacaccg gtgcgcggta gatctccggc gcaaaggggc cgaagccgct cttgtacggc atggacttgg cggtcagcgc catcgtcagg ttggtgcggc cgtggtaggc gtagtcgaac gcgaccacgg cctgcttacg ggtgtgcgcg cgggcgacct tgatggagtt ctccaccgcc tcggcgccgg agttgaacag caccgtccgc ttctcgccgg agcccggggt gatccgattg agctgttcgg cgacggccac gtattgctcg tagggggtca ccatgaagca ggtgtgggtg aactgctcca cctggtcccg taccgcgtcc accacgcggg gcgccgaatt gccgatagtc gtgacggcga tgccggagcc gaggtcgatc agccggttgc cgtcggcgtc ctccacgatt ccgccacccg cgcgaaccac gaacaccggc agggtgaccc cgacgccacc cgacaccgcc gcggcccggc gcttgttgag ctccaacgac gcggggccgg ggatttcggt gaccaagaga cgactttgat cgaggctggc caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVASLDQSR LLVTEIPGPA SLELNKRRAA AVSGGVGVTL PVFVVRAGGG IVEDADGNRL IDLGSGIAVT TIGNSAPRVV DAVRDQVEQF THTCFMVTPY EQYVAVAEQL NRITPGSGEK RTVLFNSGAE AVENSIKVAR AHTRKQAVVA FDYAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPVSY PYRDNLLDKD IATDGELAAE RAINLIDKQI GAANLAAVII EPIAGEGGFI VPADGFLPAL QRWCRDNDVV FIADEVQTGF ARTGAMFACD HENVEPDLIV TAKGIADGFP LSAVTGRAEI MDAPHTSGLG GTFGGNPVAC AAALATIETI ERDGMVERAR QIERLVMDRL LRLQAADDRL GDVRGRGAMI AMELVKSGTA EPDAALTQKL AAAAHAAGVI VLTCGMFGNV IRLLPPLTIS DELLSEGLDI LCQILAD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccagc ctcgatcaaa gtcgtctctt ggtcaccgaa atccccggcc ccgcgtcgtt ggagctcaac aagcgccggg ccgcggcggt gtcgggtggc gtcggggtca ccctgccggt gttcgtggtt cgcgcgggtg gcggaatcgt ggaggacgcc gacggcaacc ggctgatcga cctcggctcc ggcatcgccg tcacgactat cggcaattcg gcgccccgcg tggtggacgc ggtacgggac caggtggagc agttcaccca cacctgcttc atggtgaccc cctacgagca atacgtggcc gtcgccgaac agctcaatcg gatcaccccg ggctccggcg agaagcggac ggtgctgttc aactccggcg ccgaggcggt ggagaactcc atcaaggtcg cccgcgcgca cacccgtaag caggccgtgg tcgcgttcga ctacgcctac cacggccgca ccaacctgac gatggcgctg accgccaagt ccatgccgta caagagcggc ttcggcccct ttgcgccgga gatctaccgc gcaccggtgt cctaccccta ccgggacaac ctgctcgaca aagacatcgc caccgacggt gagcttgccg ccgaacgcgc catcaacctc atcgacaagc agatcggcgc cgccaacctg gccgcggtca tcatcgaacc gatcgcgggc gagggcggct tcatcgttcc ggccgacggt ttcctgcccg cgctgcagcg gtggtgccgc gacaacgacg tggtgttcat cgccgacgag gtgcagaccg gatttgcccg caccggcgcc atgttcgcgt gcgaccacga gaacgtcgag cccgacctga tcgtcaccgc caaaggcatc gccgacggat tccccttgtc ggcggtcacc ggccgggccg agatcatgga cgccccgcac accagcggct tgggcggcac cttcggcggc aacccggtcg cgtgtgcggc ggccctggcc accatcgaaa cgatcgaacg cgacggcatg gtcgaacggg cccgccagat cgaacgactg gtgatggacc gattactgcg cctgcaagcc gccgatgacc gtctcggcga tgtgcgcggc cgcggcgcca tgatcgccat ggagctggtg aaatcgggca ctgccgaacc cgacgctgcg ctcacccaaa agctggccgc cgccgcccat gccgccggcg taattgtctt gacgtgcgga atgttcggca acgtgatccg gctgctgcca ccactgacga tcagcgacga gctgctgtcc gaaggcctcg acatcctgtg ccagatcctg gccgacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MymaA.01026.b.A1.PW29968
| PURIFICATION DATe: | 1/14/2011 |
| CONCENTRATION: | 27.8mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 86 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVASLDQSR LLVTEIPGPA SLELNKRRAA AVSGGVGVTL PVFVVRAGGG IVEDADGNRL IDLGSGIAVT TIGNSAPRVV DAVRDQVEQF THTCFMVTPY EQYVAVAEQL NRITPGSGEK RTVLFNSGAE AVENSIKVAR AHTRKQAVVA FDYAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPVSY PYRDNLLDKX IATDGELAAE RAINLIDKQI GAANLAAVII EPIAGEGGFI VPADGFLPAL QRWCRDNDVV FIADEVQTGF ARTGAMFACD HENVEPDLIV TAKGIADGFP LSAVTGRAEI MDAPHTSGLG GTFGGNPVAC AAALATIETI ERDGMVERAR QIERLVMDRL LRLQAADDRL GDVRGRGAMI AMELVK
Validated NT Sequence
atttcaccag ctccatggcg atcatggcgc cgcggccgcg cacatcgccg agacggtcat cggcggcttg caggcgcagt aatcggtcca tcaccagtcg ttcgatctgg cgggcccgtt cgaccatgcc gtcgcgttcg atcgtttcga tggtggccag ggccgccgca cacgcgaccg ggttgccgcc gaaggtgccg cccaagccgc tggtgtgcgg ggcgtccatg atctcggccc ggccggtgac cgccgacaag gggaatccgt cggcgatgcc tttggcggtg acgatcaggt cgggctcgac gttctcgtgg tcgcacgcga acatggcgcc ggtgcgggca aatccggtct gcacctcgtc ggcgatgaac accacgtcgt tgtcgcggca ccaccgctgc agcgcgggca ggaaaccgtc ggccggaacg atgaagccgc cctcgcccgc gatcggttcg atgatgaccg cggccaggtt ggcggcgccg atctgcttgt cgatgaggtt gatggcgcgt tcggcggcaa gctcaccgtc ggtggcgatg tntttgtcga gcaggttgtc ccggtagggg taggacaccg gtgcgcggta gatctccggc gcaaaggggc cgaagccgct cttgtacggc atggacttgg cggtcagcgc catcgtcagg ttggtgcggc cgtggtaggc gtagtcgaac gcgaccacgg cctgcttacg ggtgtgcgcg cgggcgacct tgatggagtt ctccaccgcc tcggcgccgg agttgaacag caccgtccgc ttctcgccgg agcccggggt gatccgattg agctgttcgg cgacggccac gtattgctcg tagggggtca ccatgaagca ggtgtgggtg aactgctcca cctggtcccg taccgcgtcc accacgcggg gcgccgaatt gccgatagtc gtgacggcga tgccggagcc gaggtcgatc agccggttgc cgtcggcgtc ctccacgatt ccgccacccg cgcgaaccac gaacaccggc agggtgaccc cgacgccacc cgacaccgcc gcggcccggc gcttgttgag ctccaacgac gcggggccgg ggatttcggt gaccaagaga cgactttgat cgaggctggc caccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVASLDQSR LLVTEIPGPA SLELNKRRAA AVSGGVGVTL PVFVVRAGGG IVEDADGNRL IDLGSGIAVT TIGNSAPRVV DAVRDQVEQF THTCFMVTPY EQYVAVAEQL NRITPGSGEK RTVLFNSGAE AVENSIKVAR AHTRKQAVVA FDYAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPVSY PYRDNLLDKD IATDGELAAE RAINLIDKQI GAANLAAVII EPIAGEGGFI VPADGFLPAL QRWCRDNDVV FIADEVQTGF ARTGAMFACD HENVEPDLIV TAKGIADGFP LSAVTGRAEI MDAPHTSGLG GTFGGNPVAC AAALATIETI ERDGMVERAR QIERLVMDRL LRLQAADDRL GDVRGRGAMI AMELVKSGTA EPDAALTQKL AAAAHAAGVI VLTCGMFGNV IRLLPPLTIS DELLSEGLDI LCQILAD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCCAGC CTCGATCAAA GTCGTCTCTT GGTCACCGAA ATCCCCGGCC CCGCGTCGTT GGAGCTCAAC AAGCGCCGGG CCGCGGCGGT GTCGGGTGGC GTCGGGGTCA CCCTGCCGGT GTTCGTGGTT CGCGCGGGTG GCGGAATCGT GGAGGACGCC GACGGCAACC GGCTGATCGA CCTCGGCTCC GGCATCGCCG TCACGACTAT CGGCAATTCG GCGCCCCGCG TGGTGGACGC GGTACGGGAC CAGGTGGAGC AGTTCACCCA CACCTGCTTC ATGGTGACCC CCTACGAGCA ATACGTGGCC GTCGCCGAAC AGCTCAATCG GATCACCCCG GGCTCCGGCG AGAAGCGGAC GGTGCTGTTC AACTCCGGCG CCGAGGCGGT GGAGAACTCC ATCAAGGTCG CCCGCGCGCA CACCCGTAAG CAGGCCGTGG TCGCGTTCGA CTACGCCTAC CACGGCCGCA CCAACCTGAC GATGGCGCTG ACCGCCAAGT CCATGCCGTA CAAGAGCGGC TTCGGCCCCT TTGCGCCGGA GATCTACCGC GCACCGGTGT CCTACCCCTA CCGGGACAAC CTGCTCGACA AAGACATCGC CACCGACGGT GAGCTTGCCG CCGAACGCGC CATCAACCTC ATCGACAAGC AGATCGGCGC CGCCAACCTG GCCGCGGTCA TCATCGAACC GATCGCGGGC GAGGGCGGCT TCATCGTTCC GGCCGACGGT TTCCTGCCCG CGCTGCAGCG GTGGTGCCGC GACAACGACG TGGTGTTCAT CGCCGACGAG GTGCAGACCG GATTTGCCCG CACCGGCGCC ATGTTCGCGT GCGACCACGA GAACGTCGAG CCCGACCTGA TCGTCACCGC CAAAGGCATC GCCGACGGAT TCCCCTTGTC GGCGGTCACC GGCCGGGCCG AGATCATGGA CGCCCCGCAC ACCAGCGGCT TGGGCGGCAC CTTCGGCGGC AACCCGGTCG CGTGTGCGGC GGCCCTGGCC ACCATCGAAA CGATCGAACG CGACGGCATG GTCGAACGGG CCCGCCAGAT CGAACGACTG GTGATGGACC GATTACTGCG CCTGCAAGCC GCCGATGACC GTCTCGGCGA TGTGCGCGGC CGCGGCGCCA TGATCGCCAT GGAGCTGGTG AAATCGGGCA CTGCCGAACC CGACGCTGCG CTCACCCAAA AGCTGGCCGC CGCCGCCCAT GCCGCCGGCG TAATTGTCTT GACGTGCGGA ATGTTCGGCA ACGTGATCCG GCTGCTGCCA CCACTGACGA TCAGCGACGA GCTGCTGTCC GAAGGCCTCG ACATCCTGTG CCAGATCCTG GCCGACAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA