MyabA.01026.b
4-aminobutyrate aminotransferase (GabT)
| CENTER ID: | MyabA.01026.b |
| ORGANISM: | Mycobacterium abscessus ATCC 19977 / DSM 44196 |
| ASSOCIATED DISEASE: | Skin abscess |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01026.b.A1.GE30384 | Full length( MyabA.01026.b ) | 1 | 449 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyabA.01026.b.A1.PS00899 | Full length( MyabA.01026.b ) | 1 | 449 |
Structures
4FFC
DEPOSITED: 5/31/2012
DETERMINATION: XRay
CLONE: MyabA.01026.b.A1.GE30384
PROTEIN: MyabA.01026.b.A1.PS00899
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128761 |
| PATRIC ID: | fig|36809.5.peg.4285 |
| RefSeq: | YP_001704934.1 |
| UniProt: | B1MIQ9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDITYRLAQ KRTIVTPLPG PRSGALAERR RAAVSAGVGS TAPVYAVDAD GGVIVDADGN SFIDLGAGIA VTTVGASHPA VAAAIADQAT HFTHTCFMVT PYEGYVQVAE LLNALTPGDH DKRTALFNSG AEAVENAIKV ARLATGRPAV VAFDNAYHGR TNLTMALTAK SMPYKSQFGP FAPEVYRMPA SYPLRDEPGL TGEEAARRAI SRIETQIGAQ SLAAIIIEPI QGEGGFIVPA PGFLATLTAW ASENGVVFIA DEVQTGFART GAWFASEHEG IVPDIVTMAK GIAGGMPLSA VTGRAELMDA VYAGGLGGTY GGNPVTCAAA VAALGVMREL DLPARARAIE ASVTSRLSAL AEEVDIIGEV RGRGAMLAIE IVKPGTLEPD AALTKSIAAE ALSQGVLILT CGTFGNVIRL LPPLVIGDDL LDEGITALSD IIRAKASHQ
NT Sequence
ATGACCGACA TCACCTATCG CCTCGCCCAG AAGCGCACCA TCGTGACGCC GCTACCGGGA CCCCGGTCCG GTGCACTGGC CGAGCGCCGC CGCGCCGCGG TGTCGGCGGG TGTGGGCTCC ACCGCGCCGG TCTATGCCGT CGACGCCGAT GGCGGCGTGA TCGTCGACGC CGACGGCAAC TCCTTCATCG ACCTCGGAGC AGGCATCGCG GTGACCACCG TCGGCGCCTC CCACCCGGCC GTTGCCGCCG CAATCGCCGA CCAGGCAACC CATTTCACTC ATACCTGTTT CATGGTGACG CCCTACGAGG GGTACGTACA GGTCGCCGAG CTGCTCAACG CCCTGACCCC CGGCGATCAT GACAAGCGCA CCGCACTGTT CAACTCGGGT GCCGAGGCCG TCGAGAACGC CATCAAGGTG GCACGGCTGG CCACCGGGCG ACCCGCCGTG GTCGCATTCG ACAACGCCTA TCACGGACGC ACCAACCTGA CCATGGCACT GACCGCCAAG TCGATGCCGT ACAAGTCGCA GTTCGGGCCC TTCGCGCCCG AGGTCTACCG GATGCCCGCC TCCTACCCGT TGCGCGACGA GCCGGGGCTG ACCGGCGAGG AAGCCGCACG GCGGGCGATC TCCCGCATCG AGACCCAGAT CGGCGCGCAG TCACTGGCCG CCATCATCAT CGAACCCATC CAGGGTGAGG GCGGATTCAT CGTGCCCGCA CCGGGATTCC TCGCCACGCT TACCGCATGG GCCAGCGAAA ACGGCGTCGT CTTCATTGCC GACGAAGTGC AGACCGGATT CGCCCGTACC GGCGCGTGGT TTGCCTCCGA GCACGAGGGC ATCGTCCCGG ACATCGTCAC CATGGCCAAG GGCATCGCCG GCGGTATGCC GCTGTCGGCC GTCACCGGGC GCGCCGAGCT GATGGACGCC GTGTACGCCG GCGGCCTCGG CGGCACCTAT GGCGGCAATC CGGTGACCTG CGCGGCCGCC GTCGCCGCGC TGGGGGTCAT GCGTGAACTC GATCTGCCCG CACGTGCGCG AGCCATCGAA GCCTCGGTGA CGTCCCGGCT GAGTGCACTC GCCGAAGAGG TGGACATCAT CGGCGAGGTA CGCGGGCGCG GCGCCATGCT GGCCATCGAG ATCGTCAAAC CAGGAACGCT GGAACCCGAT GCCGCCCTCA CCAAATCCAT TGCCGCCGAA GCGCTCTCCC AGGGCGTGCT GATCCTCACC TGCGGAACCT TCGGCAACGT CATCCGCTTG CTGCCACCGC TGGTTATCGG GGACGATCTA CTCGACGAGG GCATCACCGC CCTCTCCGAC ATCATTCGTG CGAAGGCGAG CCACCAG
Details for MyabA.01026.b.A1.GE30384
| HARVESTED ON: | 9/8/2010 |
| SEQUENCED ON: | 9/17/2010 |
| EXPECTED MW: | 48kDa |
| OBSERVED MW: | 49kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 58 |
Validated AA Sequence
MPYKSQFGPF APEVYRMPAS YPLRDEPGLT GEEAARRAIS RIETQIGAQS LAAIIIEPIQ GEGGFIVPAP GFLATLTAWA SENGVVFIAD EVQTGFARTG AWFASEHEGI VPDIVTMAKG IAGGMPLSAV TGRAELMDAV YAGGLGGTYG GNPVTCAAAV AALGVMRELD LPARARAIEA SVTSRLSALA EEVDIIGEVR GRGAMLAIEI VKPGTLEPDA ALTKSIAAEA LSQGVLILTC GTFGNVIRLL PPLVIGDDLL DEGITALSDI IRAKA
Validated NT Sequence
ctcgccttcg cacgaatgat gtcggagagg gcggtgatgc cctcgtcgag tagatcgtcc ccgataacca gcggtggcag caagcggatg acgttgccga aggttccgca ggtgaggatc agcacgccct gggagagcgc ttcggcggca atggatttgg tgagggcggc atcgggttcc agcgttcctg gtttgacgat ctcgatggcc agcatggcgc cgcgcccgcg tacctcgccg atgatgtcca cctcttcggc gagtgcactc agccgggacg tcaccgaggc ttcgatggct cgcgcacgtg cgggcagatc gagttcacgc atgaccccca gcgcggcgac ggcggccgcg caggtcaccg gattgccgcc ataggtgccg ccgaggccgc cggcgtacac ggcgtccatc agctcggcgc gcccggtgac ggccgacagc ggcataccgc cggcgatgcc cttggccatg gtgacgatgt ccgggacgat gccctcgtgc tcggaggcaa accacgcgcc ggtacgggcg aatccggtct gcacttcgtc ggcaatgaag acgacgccgt tttcgctggc ccatgcggta agcgtggcga ggaatcccgg tgcgggcacg atgaatccgc cctcaccctg gatgggttcg atgatgatgg cggccagtga ctgcgcgccg atctgggtct cgatgcggga gatcgcccgc cgtgcggctt cctcgccggt cagccccggc tcgtcgcgca acgggtagga ggcgggcatc cggtagacct cgggcgcgaa gggcccgaac tgcgacttgt acggcatcga c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDITYRLA QKRTIVTPLP GPRSGALAER RRAAVSAGVG STAPVYAVDA DGGVIVDADG NSFIDLGAGI AVTTVGASHP AVAAAIADQA THFTHTCFMV TPYEGYVQVA ELLNALTPGD HDKRTALFNS GAEAVENAIK VARLATGRPA VVAFDNAYHG RTNLTMALTA KSMPYKSQFG PFAPEVYRMP ASYPLRDEPG LTGEEAARRA ISRIETQIGA QSLAAIIIEP IQGEGGFIVP APGFLATLTA WASENGVVFI ADEVQTGFAR TGAWFASEHE GIVPDIVTMA KGIAGGMPLS AVTGRAELMD AVYAGGLGGT YGGNPVTCAA AVAALGVMRE LDLPARARAI EASVTSRLSA LAEEVDIIGE VRGRGAMLAI EIVKPGTLEP DAALTKSIAA EALSQGVLIL TCGTFGNVIR LLPPLVIGDD LLDEGITALS DIIRAKASHQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgacatc acctatcgcc tcgcccagaa gcgcaccatc gtgacgccgc taccgggacc ccggtccggt gcactggccg agcgccgccg cgccgcggtg tcggcgggtg tgggctccac cgcgccggtc tatgccgtcg acgccgatgg cggcgtgatc gtcgacgccg acggcaactc cttcatcgac ctcggagcag gcatcgcggt gaccaccgtc ggcgcctccc acccggccgt tgccgccgca atcgccgacc aggcaaccca tttcactcat acctgtttca tggtgacgcc ctacgagggg tacgtacagg tcgccgagct gctcaacgcc ctgacccccg gcgatcatga caagcgcacc gcactgttca actcgggtgc cgaggccgtc gagaacgcca tcaaggtggc acggctggcc accgggcgac ccgccgtggt cgcattcgac aacgcctatc acggacgcac caacctgacc atggcactga ccgccaagtc gatgccgtac aagtcgcagt tcgggccctt cgcgcccgag gtctaccgga tgcccgcctc ctacccgttg cgcgacgagc cggggctgac cggcgaggaa gccgcacggc gggcgatctc ccgcatcgag acccagatcg gcgcgcagtc actggccgcc atcatcatcg aacccatcca gggtgagggc ggattcatcg tgcccgcacc gggattcctc gccacgctta ccgcatgggc cagcgaaaac ggcgtcgtct tcattgccga cgaagtgcag accggattcg cccgtaccgg cgcgtggttt gcctccgagc acgagggcat cgtcccggac atcgtcacca tggccaaggg catcgccggc ggtatgccgc tgtcggccgt caccgggcgc gccgagctga tggacgccgt gtacgccggc ggcctcggcg gcacctatgg cggcaatccg gtgacctgcg cggccgccgt cgccgcgctg ggggtcatgc gtgaactcga tctgcccgca cgtgcgcgag ccatcgaagc ctcggtgacg tcccggctga gtgcactcgc cgaagaggtg gacatcatcg gcgaggtacg cgggcgcggc gccatgctgg ccatcgagat cgtcaaacca ggaacgctgg aacccgatgc cgccctcacc aaatccattg ccgccgaagc gctctcccag ggcgtgctga tcctcacctg cggaaccttc ggcaacgtca tccgcttgct gccaccgctg gttatcgggg acgatctact cgacgagggc atcaccgccc tctccgacat cattcgtgcg aaggcgagcc accagtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MyabA.01026.b.A1.PS00899
| PURIFICATION DATe: | 12/9/2010 |
| CONCENTRATION: | 79.94mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 58 |
Protocol Notes
notes unavailable
Validated AA Sequence
MPYKSQFGPF APEVYRMPAS YPLRDEPGLT GEEAARRAIS RIETQIGAQS LAAIIIEPIQ GEGGFIVPAP GFLATLTAWA SENGVVFIAD EVQTGFARTG AWFASEHEGI VPDIVTMAKG IAGGMPLSAV TGRAELMDAV YAGGLGGTYG GNPVTCAAAV AALGVMRELD LPARARAIEA SVTSRLSALA EEVDIIGEVR GRGAMLAIEI VKPGTLEPDA ALTKSIAAEA LSQGVLILTC GTFGNVIRLL PPLVIGDDLL DEGITALSDI IRAKA
Validated NT Sequence
ctcgccttcg cacgaatgat gtcggagagg gcggtgatgc cctcgtcgag tagatcgtcc ccgataacca gcggtggcag caagcggatg acgttgccga aggttccgca ggtgaggatc agcacgccct gggagagcgc ttcggcggca atggatttgg tgagggcggc atcgggttcc agcgttcctg gtttgacgat ctcgatggcc agcatggcgc cgcgcccgcg tacctcgccg atgatgtcca cctcttcggc gagtgcactc agccgggacg tcaccgaggc ttcgatggct cgcgcacgtg cgggcagatc gagttcacgc atgaccccca gcgcggcgac ggcggccgcg caggtcaccg gattgccgcc ataggtgccg ccgaggccgc cggcgtacac ggcgtccatc agctcggcgc gcccggtgac ggccgacagc ggcataccgc cggcgatgcc cttggccatg gtgacgatgt ccgggacgat gccctcgtgc tcggaggcaa accacgcgcc ggtacgggcg aatccggtct gcacttcgtc ggcaatgaag acgacgccgt tttcgctggc ccatgcggta agcgtggcga ggaatcccgg tgcgggcacg atgaatccgc cctcaccctg gatgggttcg atgatgatgg cggccagtga ctgcgcgccg atctgggtct cgatgcggga gatcgcccgc cgtgcggctt cctcgccggt cagccccggc tcgtcgcgca acgggtagga ggcgggcatc cggtagacct cgggcgcgaa gggcccgaac tgcgacttgt acggcatcga c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDITYRLA QKRTIVTPLP GPRSGALAER RRAAVSAGVG STAPVYAVDA DGGVIVDADG NSFIDLGAGI AVTTVGASHP AVAAAIADQA THFTHTCFMV TPYEGYVQVA ELLNALTPGD HDKRTALFNS GAEAVENAIK VARLATGRPA VVAFDNAYHG RTNLTMALTA KSMPYKSQFG PFAPEVYRMP ASYPLRDEPG LTGEEAARRA ISRIETQIGA QSLAAIIIEP IQGEGGFIVP APGFLATLTA WASENGVVFI ADEVQTGFAR TGAWFASEHE GIVPDIVTMA KGIAGGMPLS AVTGRAELMD AVYAGGLGGT YGGNPVTCAA AVAALGVMRE LDLPARARAI EASVTSRLSA LAEEVDIIGE VRGRGAMLAI EIVKPGTLEP DAALTKSIAA EALSQGVLIL TCGTFGNVIR LLPPLVIGDD LLDEGITALS DIIRAKASHQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGACATC ACCTATCGCC TCGCCCAGAA GCGCACCATC GTGACGCCGC TACCGGGACC CCGGTCCGGT GCACTGGCCG AGCGCCGCCG CGCCGCGGTG TCGGCGGGTG TGGGCTCCAC CGCGCCGGTC TATGCCGTCG ACGCCGATGG CGGCGTGATC GTCGACGCCG ACGGCAACTC CTTCATCGAC CTCGGAGCAG GCATCGCGGT GACCACCGTC GGCGCCTCCC ACCCGGCCGT TGCCGCCGCA ATCGCCGACC AGGCAACCCA TTTCACTCAT ACCTGTTTCA TGGTGACGCC CTACGAGGGG TACGTACAGG TCGCCGAGCT GCTCAACGCC CTGACCCCCG GCGATCATGA CAAGCGCACC GCACTGTTCA ACTCGGGTGC CGAGGCCGTC GAGAACGCCA TCAAGGTGGC ACGGCTGGCC ACCGGGCGAC CCGCCGTGGT CGCATTCGAC AACGCCTATC ACGGACGCAC CAACCTGACC ATGGCACTGA CCGCCAAGTC GATGCCGTAC AAGTCGCAGT TCGGGCCCTT CGCGCCCGAG GTCTACCGGA TGCCCGCCTC CTACCCGTTG CGCGACGAGC CGGGGCTGAC CGGCGAGGAA GCCGCACGGC GGGCGATCTC CCGCATCGAG ACCCAGATCG GCGCGCAGTC ACTGGCCGCC ATCATCATCG AACCCATCCA GGGTGAGGGC GGATTCATCG TGCCCGCACC GGGATTCCTC GCCACGCTTA CCGCATGGGC CAGCGAAAAC GGCGTCGTCT TCATTGCCGA CGAAGTGCAG ACCGGATTCG CCCGTACCGG CGCGTGGTTT GCCTCCGAGC ACGAGGGCAT CGTCCCGGAC ATCGTCACCA TGGCCAAGGG CATCGCCGGC GGTATGCCGC TGTCGGCCGT CACCGGGCGC GCCGAGCTGA TGGACGCCGT GTACGCCGGC GGCCTCGGCG GCACCTATGG CGGCAATCCG GTGACCTGCG CGGCCGCCGT CGCCGCGCTG GGGGTCATGC GTGAACTCGA TCTGCCCGCA CGTGCGCGAG CCATCGAAGC CTCGGTGACG TCCCGGCTGA GTGCACTCGC CGAAGAGGTG GACATCATCG GCGAGGTACG CGGGCGCGGC GCCATGCTGG CCATCGAGAT CGTCAAACCA GGAACGCTGG AACCCGATGC CGCCCTCACC AAATCCATTG CCGCCGAAGC GCTCTCCCAG GGCGTGCTGA TCCTCACCTG CGGAACCTTC GGCAACGTCA TCCGCTTGCT GCCACCGCTG GTTATCGGGG ACGATCTACT CGACGAGGGC ATCACCGCCC TCTCCGACAT CATTCGTGCG AAGGCGAGCC ACCAGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA