SSGCID: Target Page

MyleA.01026.a

Putative 4-aminobutyrate aminotransferase

CENTER ID:	MyleA.01026.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01026.a.A1.GE34936	Full length( MyleA.01026.a )		1	446

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.845
RefSeq:	YP_002503056.1
UniProt:	A0A0H3MPK5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSVEQSRQL VTEIPGPVSL ELAKRLNAAV PRGVGVTLPV FVTRAAGGVI EDVDGNRLID LGSGIAVTTI GNSSPRVVDA VRAQVADFTH TCFIITPYEE YVAVTEQLNR ITPGSGEKRS VLFNSGAEAV ENSIKVARSY TRKPAVVAFD HAYHGRTNLT MALTAKSMPY KSGFGPFAPE IYRAPLSYPY RDGLLNKDLA TDGKLAGARA INVIEKQVGA DDLAAVIIEP IQGEGGFIVP AEGFLATLLD WCRKNNVMFI ADEVQTGFAR TGAMFACEHD GIVPDLICTA KGIADGLPLA AVTGRAEIMN APHVSGLGGT FGGNPVACAA ALATITTIEN DGLIQRAQQI ERLITDRLLR LQDADDRIGD VRGRGAMIAV ELVKSGTAEP DPELTEKVAT AAHATGVIIL TCGMFGNIIR LLPPLTISDE LLAEGLDILS RILGDF

NT Sequence

GTGACCAGCG TCGAGCAGAG CCGTCAGCTG GTCACTGAAA TCCCCGGCCC CGTGTCACTT GAACTAGCCA AACGCCTCAA CGCGGCGGTG CCTCGTGGGG TGGGAGTCAC CCTGCCAGTG TTTGTAACGC GCGCCGCCGG CGGCGTTATC GAGGACGTCG ACGGGAACCG GCTTATCGAC CTGGGTTCTG GAATCGCCGT CACCACAATC GGCAACTCGT CGCCTCGCGT TGTGGACGCG GTGCGCGCCC AGGTGGCCGA CTTTACCCAC ACCTGTTTCA TAATCACACC TTACGAGGAG TACGTCGCCG TCACCGAGCA ACTCAATCGA ATTACCCCAG GATCCGGCGA GAAACGCTCG GTGCTGTTCA ATTCCGGCGC CGAGGCAGTG GAGAATTCTA TCAAGGTCGC ACGTTCGTAC ACCCGCAAGC CCGCAGTGGT CGCGTTCGAC CACGCCTACC ACGGTCGCAC CAACCTGACA ATGGCACTGA CCGCCAAGTC CATGCCGTAC AAAAGCGGCT TTGGTCCTTT TGCGCCGGAG ATCTACCGGG CGCCGCTGTC CTACCCCTAT CGGGACGGCC TGCTCAACAA AGACCTAGCC ACCGACGGCA AGTTGGCCGG CGCACGGGCC ATCAACGTCA TTGAGAAGCA GGTTGGCGCC GATGACCTGG CTGCCGTGAT CATTGAGCCG ATCCAGGGCG AAGGCGGTTT CATCGTCCCG GCCGAAGGGT TTTTAGCTAC TTTGCTGGAT TGGTGCCGCA AGAACAACGT GATGTTCATC GCCGATGAGG TGCAAACGGG GTTTGCGCGT ACCGGCGCCA TGTTCGCCTG CGAGCACGAT GGAATCGTGC CCGACCTGAT CTGCACCGCC AAGGGCATCG CCGACGGATT GCCGCTGGCG GCGGTAACCG GACGGGCTGA GATCATGAAC GCCCCGCACG TCAGCGGCCT CGGTGGCACT TTCGGCGGCA ACCCGGTCGC CTGCGCGGCG GCTTTGGCAA CCATCACGAC CATCGAGAAC GACGGCCTGA TCCAGCGAGC CCAACAGATC GAACGGCTGA TCACCGACCG GTTGTTGCGA CTTCAGGACG CCGACGATCG GATAGGTGAC GTACGTGGCC GGGGTGCCAT GATCGCCGTC GAATTGGTAA AATCCGGCAC GGCTGAGCCC GACCCCGAGC TGACCGAAAA AGTAGCCACC GCAGCACACG CTACCGGCGT CATCATCTTG ACGTGTGGCA TGTTCGGCAA CATCATCCGA CTGCTGCCGC CTCTGACCAT AAGCGACGAA CTGTTGGCCG AAGGCCTTGA CATCCTAAGC CGGATCCTGG GTGACTTC

Details for MyleA.01026.a.A1.GE34936

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	95
PERCENT COVERAGE:	69

Validated AA Sequence

Validated NT Sequence

tnnnnnnnnn ntttgtttnn tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgac cagcgtcgag cagagccgtc agctggtcac tgaaatcccc ggccccgtgt cacttgaact agccaaacgc ctcaacgcgg cggtgcctcg tggggtggga gtcaccctgc cagtgtttgt aacgcgcgcc gccggcggcg ttatcgagga cgtcgacggg aaccggctta tcgacctggg ttctggaatc gccgtcacca caatcggcaa ctcgtcgcct cgcgttgtgg acgcggtgcg cgcccaggtg gccgacttta cccacacctg tttcataatc acaccttacg aggagtacgt cgccgtcacc gagcaactca atcgaattac cccaggatcc ggcgagaaac gctcggtgct gttcaattcc ggcgccgagg cagtggagaa ttctatcaag gtcgcacgtt cgtacacccg caagcccgca gtggtcgcgt tcgaccacgc ctaccacggt cgcaccaacc tgacaatggc actgaccgcc aagtccatgc cgtacaaaag cggctttggt ccttttgcgc cggagatcta ccgggcgccg ctgtcctacc cctatcggga cggcctgctc aacaaagacc tagccaccga cggcaagttg gccggcgcac gggccatcaa cgtcattgag aagcaggttg gcgccgatga cctggctgcc gtgatcattg agccgatcca gggcgaangc ggtttcatcg tcccggccga agggttttta gctactttgc tggattggtg ccgcaagaac aacgtgatgt tcatcgccna tgagnngcaa acggggtttg cgcgtaccgg cgccntgttc gcctgnnagc acgatggaat cgtgcccnac ctgatctgca ccgcnngggc atcgccnang gatngccgct ggcggcggta accnganggg ntganatcat gaacnncccn cangtcagcn gcnngnnggn antttcgnnn gnancnnnnn nnctnnncgg ngnnttngna nnntcacnac nntnnnnnan nnnnnntgan cnnnnnnnnc nannnnatcn nannnnngan nnncnnnncg gnnnnnnnnn nnnntnnnnn nnnnna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTSVEQSR QLVTEIPGPV SLELAKRLNA AVPRGVGVTL PVFVTRAAGG VIEDVDGNRL IDLGSGIAVT TIGNSSPRVV DAVRAQVADF THTCFIITPY EEYVAVTEQL NRITPGSGEK RSVLFNSGAE AVENSIKVAR SYTRKPAVVA FDHAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPLSY PYRDGLLNKD LATDGKLAGA RAINVIEKQV GADDLAAVII EPIQGEGGFI VPAEGFLATL LDWCRKNNVM FIADEVQTGF ARTGAMFACE HDGIVPDLIC TAKGIADGLP LAAVTGRAEI MNAPHVSGLG GTFGGNPVAC AAALATITTI ENDGLIQRAQ QIERLITDRL LRLQDADDRI GDVRGRGAMI AVELVKSGTA EPDPELTEKV ATAAHATGVI ILTCGMFGNI IRLLPPLTIS DELLAEGLDI LSRILGDF

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccagc gtcgagcaga gccgtcagct ggtcactgaa atccccggcc ccgtgtcact tgaactagcc aaacgcctca acgcggcggt gcctcgtggg gtgggagtca ccctgccagt gtttgtaacg cgcgccgccg gcggcgttat cgaggacgtc gacgggaacc ggcttatcga cctgggttct ggaatcgccg tcaccacaat cggcaactcg tcgcctcgcg ttgtggacgc ggtgcgcgcc caggtggccg actttaccca cacctgtttc ataatcacac cttacgagga gtacgtcgcc gtcaccgagc aactcaatcg aattacccca ggatccggcg agaaacgctc ggtgctgttc aattccggcg ccgaggcagt ggagaattct atcaaggtcg cacgttcgta cacccgcaag cccgcagtgg tcgcgttcga ccacgcctac cacggtcgca ccaacctgac aatggcactg accgccaagt ccatgccgta caaaagcggc tttggtcctt ttgcgccgga gatctaccgg gcgccgctgt cctaccccta tcgggacggc ctgctcaaca aagacctagc caccgacggc aagttggccg gcgcacgggc catcaacgtc attgagaagc aggttggcgc cgatgacctg gctgccgtga tcattgagcc gatccagggc gaaggcggtt tcatcgtccc ggccgaaggg tttttagcta ctttgctgga ttggtgccgc aagaacaacg tgatgttcat cgccgatgag gtgcaaacgg ggtttgcgcg taccggcgcc atgttcgcct gcgagcacga tggaatcgtg cccgacctga tctgcaccgc caagggcatc gccgacggat tgccgctggc ggcggtaacc ggacgggctg agatcatgaa cgccccgcac gtcagcggcc tcggtggcac tttcggcggc aacccggtcg cctgcgcggc ggctttggca accatcacga ccatcgagaa cgacggcctg atccagcgag cccaacagat cgaacggctg atcaccgacc ggttgttgcg acttcaggac gccgacgatc ggataggtga cgtacgtggc cggggtgcca tgatcgccgt cgaattggta aaatccggca cggctgagcc cgaccccgag ctgaccgaaa aagtagccac cgcagcacac gctaccggcg tcatcatctt gacgtgtggc atgttcggca acatcatccg actgctgccg cctctgacca taagcgacga actgttggcc gaaggccttg acatcctaag ccggatcctg ggtgacttca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a