MysmA.01026.c
4-aminobutyrate transaminase
| CENTER ID: | MysmA.01026.c |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01026.c.A1.PW28766 | Full length( MysmA.01026.c ) | 1 | 446 |
Structures
3OKS
DEPOSITED: 8/25/2010
DETERMINATION: XRay
CLONE: MysmA.01026.c.A1.GE28861
PROTEIN: MysmA.01026.c.A1.PW28766
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128761 |
| PATRIC ID: | fig|246196.19.peg.2922 |
| RefSeq: | YP_887278.1 |
| UniProt: | A0QWJ0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHPEQSRHL ATAIPGPRSQ ALIDRKGTAV ARGVGTTMPV YAVRAGGGIV EDVDGNRLID LGSGIAVTTV GNSAPKVVEA VRSQVGDFTH TCFMVTPYEG YVAVCEQLNR LTPVRGDKRS ALFNSGSEAV ENAVKIARSH THKPAVVAFD HAYHGRTNLT MALTAKVMPY KDGFGPFAPE IYRAPLSYPF RDAEFGKELA TDGELAAKRA ITVIDKQIGA DNLAAVVIEP IQGEGGFIVP ADGFLPTLLD WCRKNDVVFI ADEVQTGFAR TGAMFACEHE GIDPDLIVTA KGIAGGLPLS AVTGRAEIMD SPHVSGLGGT YGGNPIACAA ALATIETIES EGLVARAQQI EKIMKDRLGR LQAEDDRIGD VRGRGAMIAM ELVKAGTTEP DADLTKALCA GAHAAGVIVL SCGTYGNVVR FLPPLSIGDD LLNEGLDVLE EVLRGA
NT Sequence
GTGAGCCATC CCGAGCAGAG CCGCCATCTC GCCACCGCCA TACCTGGGCC GAGATCCCAG GCACTGATCG ACAGGAAGGG CACAGCCGTC GCCCGCGGCG TCGGCACCAC GATGCCGGTC TACGCGGTCA GGGCCGGCGG CGGCATAGTC GAGGATGTCG ACGGCAACCG GCTGATCGAC CTCGGATCAG GCATTGCCGT CACCACCGTG GGCAACTCCG CGCCGAAGGT CGTCGAGGCC GTGCGCAGCC AGGTCGGCGA CTTCACCCAC ACATGCTTCA TGGTGACGCC GTACGAGGGG TACGTCGCGG TCTGCGAGCA ACTCAACCGG CTGACCCCGG TCCGCGGTGA CAAACGCTCG GCCCTGTTCA ACTCGGGTTC GGAGGCCGTC GAGAACGCCG TCAAGATCGC GCGGTCCCAC ACGCACAAAC CCGCGGTCGT CGCGTTCGAT CACGCCTATC ACGGCCGCAC CAACCTGACC ATGGCGCTGA CGGCCAAGGT CATGCCGTAC AAGGACGGGT TCGGACCGTT CGCGCCGGAG ATCTACCGCG CGCCGCTTTC CTATCCCTTC CGCGATGCCG AGTTCGGCAA GGAACTGGCG ACCGACGGCG AACTGGCGGC CAAGCGCGCG ATCACCGTCA TCGACAAGCA GATCGGCGCC GACAACCTCG CCGCGGTCGT CATCGAGCCG ATCCAGGGTG AGGGTGGCTT CATCGTGCCC GCCGACGGTT TCCTGCCGAC GCTGCTCGAC TGGTGCCGCA AGAACGATGT GGTGTTCATC GCCGACGAGG TCCAGACGGG TTTCGCGCGC ACGGGCGCGA TGTTCGCGTG CGAGCACGAG GGCATCGATC CCGATCTGAT CGTCACCGCC AAGGGCATCG CAGGCGGCCT GCCGCTGTCG GCGGTCACCG GACGCGCCGA GATCATGGAC TCGCCGCACG TGTCGGGACT CGGTGGCACG TACGGCGGCA ACCCGATCGC GTGCGCGGCC GCGCTGGCGA CCATCGAGAC CATCGAATCC GAGGGGCTCG TGGCGCGTGC GCAGCAGATC GAGAAGATCA TGAAGGACCG GCTCGGACGC CTGCAGGCCG AGGACGACCG CATCGGGGAT GTGCGGGGCC GCGGCGCGAT GATCGCGATG GAACTGGTCA AGGCCGGCAC CACCGAACCC GATGCCGACC TGACCAAGGC CCTGTGCGCA GGTGCGCACG CCGCGGGGGT GATCGTATTG TCTTGCGGCA CTTACGGAAA CGTGGTGCGT TTCCTGCCGC CGTTGTCGAT CGGTGACGAT CTGCTCAACG AGGGACTCGA CGTCCTCGAA GAGGTCCTCC GCGGCGCG
Details for MysmA.01026.c.A1.PW28766
| PURIFICATION DATe: | 5/17/2010 |
| CONCENTRATION: | 25.11mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSHPEQSR HLATAIPGPR SQALIDRKGT AVARGVGTTM PVYAVRAGGG IVEDVDGNRL IDLGSGIAVT TVGNSAPKVV EAVRSQVGDF THTCFMVTPY EGYVAVCEQL NRLTPVRGDK RSALFNSGSE AVENAVKIAR SHTHKPAVVA FDHAYHGRTN LTMALTAKVM PYKDGFGPFA PEIYRAPLSY PFRDAEFGKE LATDGELAAK RAITVIDKQI GADNLAAVVI EPIQGEGGFI VPADGFLPTL LDWCRKNDVV FIADEVQTGF ARTGAMFACE HEGIDPDLIV TAKGIAGGLP LSAVTGRAEI MDSPHVSGLG GTYGGNPIAC AAALATIETI ESEGLVARAQ QIEKIMKDRL GRLQAEDDRI GDVRGRGAMI AMELVKAGTT EPDADLTKAL CAGAHAAGVI VLSCGTYGNV VRFLPPLSIG DDLLNEGLDV LEEVLRGA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGCCAT CCCGAGCAGA GCCGCCATCT CGCCACCGCC ATACCTGGGC CGAGATCCCA GGCACTGATC GACAGGAAGG GCACAGCCGT CGCCCGCGGC GTCGGCACCA CGATGCCGGT CTACGCGGTC AGGGCCGGCG GCGGCATAGT CGAGGATGTC GACGGCAACC GGCTGATCGA CCTCGGATCA GGCATTGCCG TCACCACCGT GGGCAACTCC GCGCCGAAGG TCGTCGAGGC CGTGCGCAGC CAGGTCGGCG ACTTCACCCA CACATGCTTC ATGGTGACGC CGTACGAGGG GTACGTCGCG GTCTGCGAGC AACTCAACCG GCTGACCCCG GTCCGCGGTG ACAAACGCTC GGCCCTGTTC AACTCGGGTT CGGAGGCCGT CGAGAACGCC GTCAAGATCG CGCGGTCCCA CACGCACAAA CCCGCGGTCG TCGCGTTCGA TCACGCCTAT CACGGCCGCA CCAACCTGAC CATGGCGCTG ACGGCCAAGG TCATGCCGTA CAAGGACGGG TTCGGACCGT TCGCGCCGGA GATCTACCGC GCGCCGCTTT CCTATCCCTT CCGCGATGCC GAGTTCGGCA AGGAACTGGC GACCGACGGC GAACTGGCGG CCAAGCGCGC GATCACCGTC ATCGACAAGC AGATCGGCGC CGACAACCTC GCCGCGGTCG TCATCGAGCC GATCCAGGGT GAGGGTGGCT TCATCGTGCC CGCCGACGGT TTCCTGCCGA CGCTGCTCGA CTGGTGCCGC AAGAACGATG TGGTGTTCAT CGCCGACGAG GTCCAGACGG GTTTCGCGCG CACGGGCGCG ATGTTCGCGT GCGAGCACGA GGGCATCGAT CCCGATCTGA TCGTCACCGC CAAGGGCATC GCAGGCGGCC TGCCGCTGTC GGCGGTCACC GGACGCGCCG AGATCATGGA CTCGCCGCAC GTGTCGGGAC TCGGTGGCAC GTACGGCGGC AACCCGATCG CGTGCGCGGC CGCGCTGGCG ACCATCGAGA CCATCGAATC CGAGGGGCTC GTGGCGCGTG CGCAGCAGAT CGAGAAGATC ATGAAGGACC GGCTCGGACG CCTGCAGGCC GAGGACGACC GCATCGGGGA TGTGCGGGGC CGCGGCGCGA TGATCGCGAT GGAACTGGTC AAGGCCGGCA CCACCGAACC CGATGCCGAC CTGACCAAGG CCCTGTGCGC AGGTGCGCAC GCCGCGGGGG TGATCGTATT GTCTTGCGGC ACTTACGGAA ACGTGGTGCG TTTCCTGCCG CCGTTGTCGA TCGGTGACGA TCTGCTCAAC GAGGGACTCG ACGTCCTCGA AGAGGTCCTC CGCGGCGCGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A