MyabA.01026.a

Probable 4-aminobutyrate aminotransferase (GabT)

CENTER ID: MyabA.01026.a
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.01026.a.A1.GE30383 Full length( MyabA.01026.a ) 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.859
RefSeq: YP_001701569.1
UniProt: B1MI93

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSVYSLQGG PAVAQVRRMA TEVPGPRSRE LAERRAAALP AGLVSGAGVY AAAAGGGVLV DVDGNSFLDL GSGIAVTTVG NSAPAVVARV AAQAQRFTHT CFLATPYEQY LEVAETLNRI TPGDHEKRTA LFNTGAEAVE NAVKYARVAT ARPAVVVFDH AFHGRSLMTM TMTAKQAPYR RGFGPFAPEV YRAPMAHPYR WPSGAENCAS DAFRQFASLV DNQIGAEAVA CVVVEPIQGE GGFIVPAPGF LAMVADFCRA RGILLVADEV QTGIARTGAW FASEHEQLVP DLVTTAKGLA GGLPLAAVTG RADVMDAAHP GGIGGTFSGN PLSCAAALGV FEEIETHDLL RRAADMGEML LREMRGISAE TGLIGDIRGR GAMIAAELVV PGTDSPNREA VIQILKYCQD NGVLVLSAGT YGNVLRFLPP LSMPDELLQE ALAVLRDAFR NL
NT Sequence
ATGACCTCGG TGTATAGCCT CCAGGGAGGG CCGGCAGTGG CCCAGGTGCG ACGAATGGCG ACGGAAGTGC CTGGGCCACG CTCGCGCGAG CTCGCCGAGC GCCGCGCAGC GGCGTTGCCA GCCGGCCTGG TCAGCGGTGC CGGTGTGTAT GCCGCCGCGG CGGGCGGCGG CGTACTGGTC GATGTCGACG GCAACAGCTT TCTGGATCTG GGCAGCGGCA TTGCCGTCAC CACGGTGGGC AACAGCGCAC CTGCGGTGGT TGCCCGTGTG GCCGCGCAGG CTCAACGGTT CACCCACACA TGCTTTCTGG CGACGCCCTA CGAGCAGTAC CTGGAGGTCG CCGAGACACT CAACCGAATC ACTCCCGGGG ACCACGAGAA GCGCACCGCG CTGTTCAACA CCGGTGCCGA AGCGGTTGAG AACGCGGTCA AGTACGCCCG GGTGGCGACG GCCCGCCCGG CGGTGGTTGT TTTCGATCAT GCCTTCCATG GCCGGTCGCT GATGACCATG ACCATGACCG CCAAACAGGC TCCGTACCGT CGGGGTTTCG GGCCGTTCGC TCCGGAGGTG TACCGGGCAC CCATGGCCCA CCCCTATCGG TGGCCGTCCG GAGCCGAGAA CTGCGCCTCG GACGCGTTCC GCCAGTTCGC GTCCCTGGTC GACAACCAGA TCGGTGCCGA AGCGGTGGCC TGTGTGGTGG TGGAGCCGAT CCAGGGCGAG GGCGGCTTTA TCGTCCCGGC GCCCGGATTC TTGGCGATGG TTGCCGACTT CTGCCGGGCG CGTGGCATCC TGTTGGTCGC CGACGAAGTC CAAACCGGTA TTGCCCGGAC CGGTGCATGG TTCGCCTCCG AGCACGAACA GCTGGTACCC GACCTCGTCA CCACCGCGAA GGGGCTTGCG GGCGGACTGC CTCTGGCTGC GGTAACCGGC CGCGCCGACG TCATGGACGC CGCGCATCCG GGTGGTATCG GTGGAACCTT CAGTGGCAAC CCGCTTTCCT GTGCGGCGGC ACTCGGCGTC TTCGAAGAGA TCGAGACCCA CGACCTGCTC AGGCGTGCCG CCGATATGGG CGAGATGTTG CTTCGGGAGA TGCGTGGCAT CTCCGCGGAG ACCGGCCTGA TCGGAGACAT CCGTGGGCGC GGCGCGATGA TCGCTGCCGA GCTGGTGGTG CCGGGCACCG ACTCGCCGAA TCGTGAAGCG GTGATACAGA TCTTGAAGTA TTGCCAGGAC AACGGTGTGC TGGTGCTTTC TGCTGGAACG TACGGCAATG TGCTGCGATT CCTGCCGCCG CTGTCCATGC CGGACGAGCT GCTGCAAGAG GCCCTCGCCG TGCTGCGCGA CGCGTTCCGG AACCTC
Details for MyabA.01026.a.A1.GE30383
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 49kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 71
Validated AA Sequence
MTSVYSLQGG PAVAQVRRMA TEVPGPRSRE LAERRAAALP AGLVSGAGVY AAAAGGGVLV DVDGNSFLDL GSGIAVTTVG NSAPAVVARV AAQAQRFTHT CFLATPYEQY LEVAETLNRI TPGDHEKRTA LFNTGAEAVE NAVKYARVAT ARPAVVVFDH AFHGRSLMTM TMTAKQAPYR RGFGPFAPEV YRAPMAHPYR WPSGAENCAS DAFRQFASLV DNQIGAEAVA CVVVEPIQGE GGFIVPAPGF LAMVADFCRA RGILLVADEV QTGIARTGAW FASEHEQXVP DLVTTAKGLA GGLPLAAVTG RADVMDAAHP GGIGGTFSGN PLSCAA
Validated NT Sequence
gccgccgcac aggaaagcgg gttgccactg aaggttccac cgataccacc cggatgcgcg gcgtccatga cgtcggcgcg gccggttacc gcagccagag gcagtccgcc cgcaagcccc ttcgcggtgg tgacgaggtc gggtaccanc tgttcgtgct cggaggcgaa ccatgcaccg gtccgggcaa taccggtttg gacttcgtcg gcgaccaaca ggatgccacg cgcccggcag aagtcggcaa ccatcgccaa gaatccgggc gccgggacga taaagccgcc ctcgccctgg atcggctcca ccaccacaca ggccaccgct tcggcaccga tctggttgtc gaccagggac gcgaactggc ggaacgcgtc cgaggcgcag ttctcggctc cggacggcca ccgatagggg tgggccatgg gtgcccggta cacctccgga gcgaacggcc cgaaaccccg acggtacgga gcctgtttgg cggtcatggt catggtcatc agcgaccggc catggaaggc atgatcgaaa acaaccaccg ccgggcgggc cgtcgccacc cgggcgtact tgaccgcgtt ctcaaccgct tcggcaccgg tgttgaacag cgcggtgcgc ttctcgtggt ccccgggagt gattcggttg agtgtctcgg cgacctccag gtactgctcg tagggcgtcg ccagaaagca tgtgtgggtg aaccgttgag cctgcgcggc cacacgggca accaccgcag gtgcgctgtt gcccaccgtg gtgacggcaa tgccgctgcc cagatccaga aagctgttgc cgtcgacatc gaccagtacg ccgccgcccg ccgcggcggc atacacaccg gcaccgctga ccaggccggc tggcaacgcc gctgcgcggc gctcggcgag ctcgcgcgag cgtggcccag gcacttccgt cgccattcgt cgcacctggg ccactgccgg ccctccctgg aggctataca ccgaggtcat cgaaccagga ccctgggtct gagcttccag g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSVYSLQG GPAVAQVRRM ATEVPGPRSR ELAERRAAAL PAGLVSGAGV YAAAAGGGVL VDVDGNSFLD LGSGIAVTTV GNSAPAVVAR VAAQAQRFTH TCFLATPYEQ YLEVAETLNR ITPGDHEKRT ALFNTGAEAV ENAVKYARVA TARPAVVVFD HAFHGRSLMT MTMTAKQAPY RRGFGPFAPE VYRAPMAHPY RWPSGAENCA SDAFRQFASL VDNQIGAEAV ACVVVEPIQG EGGFIVPAPG FLAMVADFCR ARGILLVADE VQTGIARTGA WFASEHEQLV PDLVTTAKGL AGGLPLAAVT GRADVMDAAH PGGIGGTFSG NPLSCAAALG VFEEIETHDL LRRAADMGEM LLREMRGISA ETGLIGDIRG RGAMIAAELV VPGTDSPNRE AVIQILKYCQ DNGVLVLSAG TYGNVLRFLP PLSMPDELLQ EALAVLRDAF RNL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacctcggtg tatagcctcc agggagggcc ggcagtggcc caggtgcgac gaatggcgac ggaagtgcct gggccacgct cgcgcgagct cgccgagcgc cgcgcagcgg cgttgccagc cggcctggtc agcggtgccg gtgtgtatgc cgccgcggcg ggcggcggcg tactggtcga tgtcgacggc aacagctttc tggatctggg cagcggcatt gccgtcacca cggtgggcaa cagcgcacct gcggtggttg cccgtgtggc cgcgcaggct caacggttca cccacacatg ctttctggcg acgccctacg agcagtacct ggaggtcgcc gagacactca accgaatcac tcccggggac cacgagaagc gcaccgcgct gttcaacacc ggtgccgaag cggttgagaa cgcggtcaag tacgcccggg tggcgacggc ccgcccggcg gtggttgttt tcgatcatgc cttccatggc cggtcgctga tgaccatgac catgaccgcc aaacaggctc cgtaccgtcg gggtttcggg ccgttcgctc cggaggtgta ccgggcaccc atggcccacc cctatcggtg gccgtccgga gccgagaact gcgcctcgga cgcgttccgc cagttcgcgt ccctggtcga caaccagatc ggtgccgaag cggtggcctg tgtggtggtg gagccgatcc agggcgaggg cggctttatc gtcccggcgc ccggattctt ggcgatggtt gccgacttct gccgggcgcg tggcatcctg ttggtcgccg acgaagtcca aaccggtatt gcccggaccg gtgcatggtt cgcctccgag cacgaacagc tggtacccga cctcgtcacc accgcgaagg ggcttgcggg cggactgcct ctggctgcgg taaccggccg cgccgacgtc atggacgccg cgcatccggg tggtatcggt ggaaccttca gtggcaaccc gctttcctgt gcggcggcac tcggcgtctt cgaagagatc gagacccacg acctgctcag gcgtgccgcc gatatgggcg agatgttgct tcgggagatg cgtggcatct ccgcggagac cggcctgatc ggagacatcc gtgggcgcgg cgcgatgatc gctgccgagc tggtggtgcc gggcaccgac tcgccgaatc gtgaagcggt gatacagatc ttgaagtatt gccaggacaa cggtgtgctg gtgctttctg ctggaacgta cggcaatgtg ctgcgattcc tgccgccgct gtccatgccg gacgagctgc tgcaagaggc cctcgccgtg ctgcgcgacg cgttccggaa cctctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa