MyavA.01026.a

4-aminobutyrate transaminase

CENTER ID: MyavA.01026.a
ORGANISM: Mycobacterium avium 104
ASSOCIATED DISEASE: Johne's disease, paratuberculosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyavA.01026.a.A1.GE29043 Full length( MyavA.01026.a ) 1 449
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|243243.7.peg.3396
RefSeq: YP_882652.1
UniProt: A0A0H2ZRI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARLEQTRRL VTEIPGPASL ELSKRRAAAV SHAVNVSVPV FVARAGGGIV EDVDGNRLID LGSGIAVTTI GNSAPRVVDA VRAQVAEFTH TCFMVTPYES YVAVAETLNR ITPGAGEKRS VLFNSGAEAV ENAVKVARAY TRKPAVVAFD HAYHGRTNLA MALTAKSMPY KGGFGPFAPE IYRAPLSYPY RDGLLDKELA TDGEKAAARA INIIDGQIGA DSLAAVIIEP IQGEGGFIVP AEGFLPTLLD WCRRNDVLFI ADEVQTGFAR SGAMFACEHE GPNGLEPDLI CTAKAIADGL PLSAVTGRAQ IMDAPHVGGL GGTFGGNPLA CAAALATIET IESDGLIERA RQLERLITEP LLRLQARDDR IGDVRGRGAM IAIELVKSGT AEPDKDLTQR LSVAAHAAGV IVLTCGMFGN IIRLLPPLTI SDELLAEGID ILGGLLADL
NT Sequence
GTGGCCAGGC TCGAGCAGAC TCGCCGACTG GTCACCGAGA TCCCCGGCCC GGCGTCGCTG GAACTGAGCA AACGCCGCGC GGCCGCGGTG TCGCACGCGG TGAACGTCTC GGTGCCGGTG TTCGTCGCCC GCGCCGGCGG CGGCATCGTC GAGGACGTCG ACGGCAACCG GCTCATCGAC CTCGGCTCCG GCATCGCGGT GACCACCATC GGCAATTCGG CGCCCCGGGT GGTCGACGCG GTGCGCGCCC AGGTGGCCGA GTTCACCCAC ACCTGCTTCA TGGTGACGCC GTACGAGTCC TACGTCGCCG TCGCCGAAAC GCTCAACCGG ATCACCCCCG GGGCCGGTGA AAAGCGCTCG GTGCTGTTCA ACTCCGGCGC CGAGGCCGTC GAGAACGCCG TCAAGGTCGC CCGCGCCTAC ACCCGTAAGC CGGCCGTGGT GGCCTTCGAC CACGCCTACC ACGGCCGCAC CAACCTGGCG ATGGCGCTGA CCGCGAAGTC GATGCCCTAC AAGGGCGGCT TCGGCCCGTT CGCGCCGGAG ATCTACCGGG CGCCGCTGTC CTACCCCTAC CGGGACGGGC TGCTCGACAA GGAACTGGCC ACCGACGGGG AGAAGGCCGC CGCGCGGGCC ATCAACATCA TCGACGGCCA GATCGGCGCC GACAGCCTGG CCGCGGTGAT CATCGAACCG ATCCAGGGCG AGGGCGGGTT CATCGTTCCC GCCGAGGGAT TCCTGCCCAC CCTGCTGGAC TGGTGCCGCC GCAACGACGT GCTGTTCATC GCCGACGAGG TGCAGACCGG GTTCGCGCGG TCCGGCGCCA TGTTCGCCTG CGAGCACGAG GGCCCGAATG GTTTAGAGCC GGACCTGATC TGCACCGCCA AGGCCATCGC CGACGGGCTG CCGCTGTCGG CGGTCACCGG CCGCGCCCAG ATCATGGACG CCCCGCACGT CGGGGGCCTG GGCGGCACCT TCGGCGGCAA CCCGCTGGCG TGCGCGGCGG CGCTGGCCAC CATCGAGACG ATCGAGAGCG ACGGCCTGAT CGAACGGGCC CGGCAGCTGG AACGCCTGAT CACCGAACCG CTGCTGCGCC TGCAGGCCCG CGACGACCGG ATCGGCGACG TCCGCGGCCG GGGCGCCATG ATCGCCATCG AACTGGTGAA GTCCGGCACC GCCGAGCCGG ACAAGGACCT GACCCAGCGG CTGTCCGTCG CCGCGCACGC GGCCGGCGTC ATCGTGCTCA CCTGCGGCAT GTTCGGCAAC ATCATCCGGC TGCTGCCGCC GCTGACCATC AGCGACGAGC TGCTCGCCGA GGGCATCGAC ATTCTCGGCG GGTTGTTGGC CGACCTG
Details for MyavA.01026.a.A1.GE29043
HARVESTED ON: 4/9/2010
SEQUENCED ON: 4/29/2010
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 95
Validated AA Sequence
MVARLEQTRR LVTEIPGPAS LELSKRRAAA VSHAXNVSVP VFVARAGGGI VEDVDGNRLI DLGSGIAVTT IGNSAPRVVD AVRAQVAEFT HTCFMVTPYE SYVAVAETLN RITPGAGEKR SXLFNSGAEA VENAVKVARA YXRKPAVVAF XHAYHGRTNL AMALTAKSMP YKGGFGPFAP EIYRAPLSYP YRDGLLDKEL ATDGEKAAAR AINIIDXQIG ADSLAAVIIE PIQGEGGFIV PAEGFLPTXL DWCRRNDVLF IADEVQTGFA RSGAMFACEH EGPNGLEPDL ICTAKAIADG LPLSAVTGRA QIMDAPHVGG LGGTFGGNPL ACAAALATIE TIESDGLIER ARQLERLITE PLLRLQARDD RIGDVRGRGA MIAIELVKSG TAEPDKDLTQ RLSVAAHAAG VIVLTCGMFG NIIRLLPPLT ISDELLAEGI DILGGLLA
Validated NT Sequence
cggccaacaa cccgccgaga atgtcgatgc cctcggcgag cagctcgtcg ctgatggtca gcggcggcag cagccggatg atgttgccga acatgccgca ggtgagcacg atgacgccgg ccgcgtgcgc ggcgacggac agccgctggg tcaggtcctt gtccggctcg gcggtgccgg acttcaccag ttcgatggcg atcatggcgc cccggccgcg gacgtcgccg atccggtcgt cgcgggcctg caggcgcagc agcggttcgg tgatcaggcg ttccagctgc cgggcccgtt cgatcaggcc gtcgctctcg atcgtctcga tggtggccag cgccgccgcg cacgccagcg ggttgccgcc gaaggtgccg cccaggcccc cgacgtgcgg ggcgtccatg atctgggcgc ggccggtgac cgccgacagc ggcagcccgt cggcgatggc cttggcggtg cagatcaggt ccggctctaa accattcggg ccctcgtgct cgcaggcgaa catggcgccg gaccgcgcga acccggtctg cacctcgtcg gcgatgaaca gcacgtcgtt gcggcggcac cagtccagca nggtgggcag gaatccctcg gcgggaacga tgaacccgcc ctcgccctgg atcggttcga tgatcaccgc ggccaggctg tcggcgccga tctggncgtc gatgatgttg atggcccgcg cggcggcctt ctccccgtcg gtggccagtt ccttgtcgag cagcccgtcc cggtaggggt aggacagcgg cgcccggtag atctccggcg cgaacgggcc gaagccgccc ttgtagggca tngacttcgc ggtcagcgcc atcgccaggt tggtgcggcc gtggtaggcg tggtngaagg ccaccacggc cggcttacgg gngtaggcgc gggcgacctt gacggcgttc tcgacggcct cggcgccgga gttgaacagc nccgagcgct tttcaccggc cccgggggtg atccggttga gcgtttcggc gacggcgacg taggactcgt acggcgtcac catgaagcag gtgtgggtga actcggccac ctgggcgcgc accgcgtcga ccacccgggg cgccgaattg ccgatggtgg tcaccgcgat gccggagccg aggtcgatga gccggttgcc gtcgacgtcc tcgacgatgc cgccgccggc gcgggcgacg aacaccggca ccgagacgtt cnncgcgtgc gacaccgcgg ccgcgcggcg tttgctcagt tccagcgacg ccgggccggg gatctcggtg accagtcggc gagtctgctc gagcctggcc accatcgaac caggaccctg ggtctgagct tccagggtac cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVARLEQTR RLVTEIPGPA SLELSKRRAA AVSHAVNVSV PVFVARAGGG IVEDVDGNRL IDLGSGIAVT TIGNSAPRVV DAVRAQVAEF THTCFMVTPY ESYVAVAETL NRITPGAGEK RSVLFNSGAE AVENAVKVAR AYTRKPAVVA FDHAYHGRTN LAMALTAKSM PYKGGFGPFA PEIYRAPLSY PYRDGLLDKE LATDGEKAAA RAINIIDGQI GADSLAAVII EPIQGEGGFI VPAEGFLPTL LDWCRRNDVL FIADEVQTGF ARSGAMFACE HEGPNGLEPD LICTAKAIAD GLPLSAVTGR AQIMDAPHVG GLGGTFGGNP LACAAALATI ETIESDGLIE RARQLERLIT EPLLRLQARD DRIGDVRGRG AMIAIELVKS GTAEPDKDLT QRLSVAAHAA GVIVLTCGMF GNIIRLLPPL TISDELLAEG IDILGGLLAD L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccagg ctcgagcaga ctcgccgact ggtcaccgag atccccggcc cggcgtcgct ggaactgagc aaacgccgcg cggccgcggt gtcgcacgcg gtgaacgtct cggtgccggt gttcgtcgcc cgcgccggcg gcggcatcgt cgaggacgtc gacggcaacc ggctcatcga cctcggctcc ggcatcgcgg tgaccaccat cggcaattcg gcgccccggg tggtcgacgc ggtgcgcgcc caggtggccg agttcaccca cacctgcttc atggtgacgc cgtacgagtc ctacgtcgcc gtcgccgaaa cgctcaaccg gatcaccccc ggggccggtg aaaagcgctc ggtgctgttc aactccggcg ccgaggccgt cgagaacgcc gtcaaggtcg cccgcgccta cacccgtaag ccggccgtgg tggccttcga ccacgcctac cacggccgca ccaacctggc gatggcgctg accgcgaagt cgatgcccta caagggcggc ttcggcccgt tcgcgccgga gatctaccgg gcgccgctgt cctaccccta ccgggacggg ctgctcgaca aggaactggc caccgacggg gagaaggccg ccgcgcgggc catcaacatc atcgacggcc agatcggcgc cgacagcctg gccgcggtga tcatcgaacc gatccagggc gagggcgggt tcatcgttcc cgccgaggga ttcctgccca ccctgctgga ctggtgccgc cgcaacgacg tgctgttcat cgccgacgag gtgcagaccg ggttcgcgcg gtccggcgcc atgttcgcct gcgagcacga gggcccgaat ggtttagagc cggacctgat ctgcaccgcc aaggccatcg ccgacgggct gccgctgtcg gcggtcaccg gccgcgccca gatcatggac gccccgcacg tcgggggcct gggcggcacc ttcggcggca acccgctggc gtgcgcggcg gcgctggcca ccatcgagac gatcgagagc gacggcctga tcgaacgggc ccggcagctg gaacgcctga tcaccgaacc gctgctgcgc ctgcaggccc gcgacgaccg gatcggcgac gtccgcggcc ggggcgccat gatcgccatc gaactggtga agtccggcac cgccgagccg gacaaggacc tgacccagcg gctgtccgtc gccgcgcacg cggccggcgt catcgtgctc acctgcggca tgttcggcaa catcatccgg ctgctgccgc cgctgaccat cagcgacgag ctgctcgccg agggcatcga cattctcggc gggttgttgg ccgacctgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa