MyulA.01026.a

4-aminobutyrate aminotransferase GabT

CENTER ID: MyulA.01026.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01026.a.A1.GE29757 Full length( MyulA.01026.a ) 1 446
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.2069
RefSeq: YP_905679.1
UniProt: A0PPD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASLDQSRLL VTEIPGPASL ELNKRRAAAV SGGVGVTLPV FVVRAGGGIV EDADGNRLID LGSGIAVTTI GNSAPRVVDA VRDQVEQFTH TCFMVIPYEH YVAVAEQLNR ITPGSGEKRT VLFNSGAEAV ENSIKVARAH TRKQAVVAFD YAYHGRTNLT MALTAKSMPY KSGFGPFAPE IYRAPVSYPY RDNLLDKDIA TDGELAAERA INLIDKQIGA ANLAAVIIEP IAGEGGFIVP ADGFLPALQR WSRDNDVVFI ADEVQTGFAR TGAMFACDHE NVEPDLIVTA KGIADGFPLS AVTGRAEIMD APHTSGLGGT FGGNPVACAA ALATIETIER DGMVERARQI ERLVMDRLLR LQAADDRLGD VRGRGAMIAM ELVKSGTAEP DAALTQKLAA AAHAAGVIVL TCGMFGNVIR LLPPLTISDE LLSEGLDILC QILADL
NT Sequence
GTGGCCAGCC TCGATCAAAG TCGTCTCTTG GTCACCGAAA TCCCCGGCCC CGCGTCGTTG GAGCTCAACA AGCGCCGGGC CGCGGCGGTG TCGGGTGGCG TCGGGGTCAC CCTGCCGGTG TTCGTGGTTC GCGCGGGTGG CGGAATCGTG GAGGACGCCG ACGGCAACCG GCTGATCGAC CTCGGCTCCG GCATCGCCGT CACGACTATC GGCAATTCGG CGCCCCGCGT GGTGGACGCG GTACGGGACC AGGTGGAGCA GTTCACCCAC ACCTGCTTCA TGGTGATCCC CTACGAGCAT TACGTGGCCG TCGCCGAACA GCTCAATCGG ATCACCCCGG GCTCCGGCGA GAAGCGGACG GTGCTGTTCA ACTCCGGCGC CGAGGCGGTG GAGAACTCCA TCAAGGTCGC CCGCGCGCAC ACCCGTAAGC AGGCCGTGGT CGCGTTCGAC TACGCCTACC ACGGCCGCAC CAACCTGACG ATGGCGCTGA CCGCCAAGTC CATGCCGTAC AAGAGCGGCT TCGGCCCCTT TGCGCCGGAG ATCTACCGCG CACCGGTGTC CTACCCCTAC CGGGACAACC TGCTCGACAA AGACATCGCC ACCGACGGTG AGCTTGCCGC CGAACGCGCC ATCAACCTCA TCGACAAGCA GATCGGCGCC GCCAACCTGG CCGCGGTCAT CATCGAACCG ATCGCGGGCG AGGGCGGCTT CATCGTTCCG GCCGACGGTT TCCTGCCCGC GCTGCAGCGG TGGTCCCGCG ACAACGACGT GGTGTTCATC GCCGACGAGG TGCAGACCGG ATTTGCCCGC ACCGGCGCCA TGTTCGCGTG CGACCACGAG AACGTCGAGC CCGACCTGAT CGTCACCGCC AAAGGCATCG CCGACGGATT CCCCTTGTCG GCGGTCACCG GCCGGGCCGA GATCATGGAC GCCCCGCACA CCAGCGGCTT GGGCGGCACC TTCGGCGGCA ACCCGGTCGC GTGTGCGGCG GCCCTGGCCA CCATCGAAAC GATCGAACGC GACGGCATGG TCGAACGGGC CCGCCAGATC GAACGACTGG TGATGGACCG ATTGCTGCGC CTGCAAGCCG CCGATGACCG TCTCGGCGAT GTGCGCGGCC GCGGCGCCAT GATCGCCATG GAGCTGGTGA AATCGGGCAC TGCCGAACCC GACGCTGCGC TCACCCAAAA GCTGGCCGCC GCCGCCCATG CTGCCGGCGT AATTGTCTTG ACGTGCGGAA TGTTCGGCAA CGTGATCCGG CTGCTGCCAC CACTGACGAT CAGCGACGAG CTGCTGTCCG AAGGCCTCGA CATCCTGTGC CAGATCCTGG CCGACCTC
Details for MyulA.01026.a.A1.GE29757
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 75
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVASLDQSR LLVTEIPGPA SLELNKRRAA AVSGGVGVTL PVFVVRAGGG IVEDADGNRL IDLGSGIAVT TIGNSAPRVV DAVRDQVEQF THTCFMVIPY EHYVAVAEQL NRITPGSGEK RTVLFNSGAE AVENSIKVAR AHTRKQAVVA FDYAYHGRTN LTMALTAKSM PYKSGFGPFA PXIYRAPVSY PYRXXCSXKT SXPXXXLXPX XXXSXXXXXX XXXXXXXXGX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXX
Validated NT Sequence
annnnnnnnn nnnntttgtt tactttaaga aggaganata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggt ggccagcctc gatcaaagtc gtctcttggt caccgaaatc cccggccccg cgtcgttgga gctcaacaag cgccgggccg cggcggtgtc gggtggcgtc ggggtcaccc tgccggtgtt cgtggttcgc gcgggtggcg gaatcgtgga ggacgccgac ggcaaccggc tgatcgacct cggctccggc atcgccgtca cgactatcgg caattcggcg ccccgcgtgg tggacgcggt acgggaccag gtggagcagt tcacccacac ctgcttcatg gtgatcccct acgagcatta cgtggccgtc gccgaacagc tcaatcggat caccccgggc tccggcgaga agcggacggt gctgttcaac tccggcgccg aggcggtgga gaactccatc aaggtcgccc gcgcgcacac ccgtaagcag gccgtggtcg cgttcgacta cgcctaccac ggccgcacca acctgacgat ggcgctgacc gccaagtcca tgccgtacaa gagcggcttc ggcccctttg cgccgganat ctaccgcgca ccggtgtcct acccctaccg ggananctgc tcganaaaga cntcgcnncc gangnnganc ttgcngccna annnncntnc atcgananca natngnncnn nnnnnnnnng tcntcntcna nnatngggnn nngnnnntnn tcntnnnnan nnncnnnnnn nnnnnnnnnn ncnnnnnnnn nnnnnnnnnn cnnnnnnnnn nnnnnnnntn cnnnnnnnnn nnnnaannnn nnnnnncnnn nnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVASLDQSR LLVTEIPGPA SLELNKRRAA AVSGGVGVTL PVFVVRAGGG IVEDADGNRL IDLGSGIAVT TIGNSAPRVV DAVRDQVEQF THTCFMVIPY EHYVAVAEQL NRITPGSGEK RTVLFNSGAE AVENSIKVAR AHTRKQAVVA FDYAYHGRTN LTMALTAKSM PYKSGFGPFA PEIYRAPVSY PYRDNLLDKD IATDGELAAE RAINLIDKQI GAANLAAVII EPIAGEGGFI VPADGFLPAL QRWSRDNDVV FIADEVQTGF ARTGAMFACD HENVEPDLIV TAKGIADGFP LSAVTGRAEI MDAPHTSGLG GTFGGNPVAC AAALATIETI ERDGMVERAR QIERLVMDRL LRLQAADDRL GDVRGRGAMI AMELVKSGTA EPDAALTQKL AAAAHAAGVI VLTCGMFGNV IRLLPPLTIS DELLSEGLDI LCQILADL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccagc ctcgatcaaa gtcgtctctt ggtcaccgaa atccccggcc ccgcgtcgtt ggagctcaac aagcgccggg ccgcggcggt gtcgggtggc gtcggggtca ccctgccggt gttcgtggtt cgcgcgggtg gcggaatcgt ggaggacgcc gacggcaacc ggctgatcga cctcggctcc ggcatcgccg tcacgactat cggcaattcg gcgccccgcg tggtggacgc ggtacgggac caggtggagc agttcaccca cacctgcttc atggtgatcc cctacgagca ttacgtggcc gtcgccgaac agctcaatcg gatcaccccg ggctccggcg agaagcggac ggtgctgttc aactccggcg ccgaggcggt ggagaactcc atcaaggtcg cccgcgcgca cacccgtaag caggccgtgg tcgcgttcga ctacgcctac cacggccgca ccaacctgac gatggcgctg accgccaagt ccatgccgta caagagcggc ttcggcccct ttgcgccgga gatctaccgc gcaccggtgt cctaccccta ccgggacaac ctgctcgaca aagacatcgc caccgacggt gagcttgccg ccgaacgcgc catcaacctc atcgacaagc agatcggcgc cgccaacctg gccgcggtca tcatcgaacc gatcgcgggc gagggcggct tcatcgttcc ggccgacggt ttcctgcccg cgctgcagcg gtggtcccgc gacaacgacg tggtgttcat cgccgacgag gtgcagaccg gatttgcccg caccggcgcc atgttcgcgt gcgaccacga gaacgtcgag cccgacctga tcgtcaccgc caaaggcatc gccgacggat tccccttgtc ggcggtcacc ggccgggccg agatcatgga cgccccgcac accagcggct tgggcggcac cttcggcggc aacccggtcg cgtgtgcggc ggccctggcc accatcgaaa cgatcgaacg cgacggcatg gtcgaacggg cccgccagat cgaacgactg gtgatggacc gattgctgcg cctgcaagcc gccgatgacc gtctcggcga tgtgcgcggc cgcggcgcca tgatcgccat ggagctggtg aaatcgggca ctgccgaacc cgacgctgcg ctcacccaaa agctggccgc cgccgcccat gctgccggcg taattgtctt gacgtgcgga atgttcggca acgtgatccg gctgctgcca ccactgacga tcagcgacga gctgctgtcc gaaggcctcg acatcctgtg ccagatcctg gccgacctca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a