MytuD.00781.a

acetyl-CoA acetyltransferase

CENTER ID: MytuD.00781.a
ORGANISM: Mycobacterium tuberculosis H37Rv
ASSOCIATED DISEASE: Tuberculosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MytuD.00781.a.A1.GE45909 Full length( MytuD.00781.a ) 1 405
MytuD.00781.a.B1.GE27169 Full length( MytuD.00781.a ) 1 405
MytuD.00781.a.B1.GE27590 Full length( MytuD.00781.a ) 1 405
MytuD.00781.a.HZ1.GE45910 Full length( MytuD.00781.a ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|83332.12.peg.1200
RefSeq: NP_215590.1
TubercuList: Rv1074c
MTB Network Portal: Rv1074c
UniProt: O53422

Orthologues for Rv1074c

Species % Identity % Coverage Clones Proteins Structures
marinum 93 100 1 0 0
ulcerans 92 100 1 0 0
avium 91 100 1 0 0
paratuberculosis 91 68 1 0 0
smegmatis 84 100 1 1 0
thermoresistibile 84 97 1 0 0
abscessus 83 100 1 1 0
paratuberculosis 40 99 0 0 0
marinum 39 99 0 0 0
ulcerans 39 99 1 0 0
thermoresistibile 38 78 0 0 0
thermoresistibile 37 87 1 0 0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEAVIVSTA RSPIGRAMKG SLVGMRPDDL AVQMVRAALD KVPALNPHQI DDLMMGCGLP GGESGFNIAR VVAVALGYDF LPGTTVNRYC SSSLQTTRMA FHAIKAGEGD AFISAGVETV SRFAKGNSDS WPDTKNPLFD GAQERSAAAA AGADEWHDPR TDQKLPDIYI AMGQTAENVA IMTGISREEQ DRWGVRSQNR AEEAIKNGFF EREITPVTLP DGTTVSTDDG PRPGTTYEKV SELKPAFRPN GTVTAGNACP LNDGAAAVVI TSDTKAKELG LTPLARIVST GVSGLSPEIM GLGPIEASKK ALERAGMAIT DIDLVEINEA FAVQVLGSAR ELGIDEDKLN ISGGAIALGH PFGMTGARIT TTLLNNLQTY DKTFGLETMC VGGGQGMAMV IERLA
NT Sequence
ATGCCAGAAG CTGTCATCGT CTCGACTGCC CGCTCGCCGA TTGGCCGGGC CATGAAAGGA TCGCTGGTCG GCATGCGGCC CGACGACCTG GCCGTCCAGA TGGTGCGCGC CGCGCTCGAC AAGGTTCCGG CACTGAACCC CCATCAGATC GACGACCTCA TGATGGGCTG TGGCCTGCCA GGCGGTGAGT CCGGTTTCAA CATCGCCCGT GTCGTCGCCG TCGCGCTCGG CTATGACTTC CTGCCGGGCA CCACGGTCAA CCGGTACTGT TCGTCGTCGC TGCAGACCAC CCGGATGGCC TTCCACGCGA TCAAGGCCGG TGAGGGCGAC GCGTTCATCT CCGCGGGCGT GGAAACCGTC TCCCGGTTCG CCAAGGGAAA CTCCGACTCC TGGCCGGACA CCAAGAACCC GCTGTTCGAC GGGGCTCAGG AACGTTCGGC CGCGGCAGCC GCCGGCGCCG ACGAATGGCA CGATCCCCGC ACCGATCAGA AGCTGCCGGA CATCTATATT GCGATGGGCC AGACCGCGGA AAACGTCGCC ATCATGACCG GCATCAGCCG CGAAGAACAG GACCGCTGGG GCGTGCGCAG CCAGAACCGG GCCGAAGAGG CGATCAAGAA CGGGTTCTTC GAACGAGAAA TCACGCCGGT CACCCTTCCG GACGGCACCA CGGTCAGCAC CGACGACGGC CCGCGACCGG GTACCACCTA CGAAAAGGTC AGCGAGCTCA AGCCGGCTTT CCGGCCGAAT GGCACCGTGA CCGCGGGGAA TGCCTGTCCG CTCAACGACG GGGCCGCCGC GGTGGTGATC ACCAGCGACA CCAAGGCCAA GGAATTAGGC TTGACACCGC TGGCGCGCAT TGTGTCCACC GGGGTCAGCG GGCTGTCCCC GGAGATCATG GGCTTAGGGC CGATCGAGGC GTCCAAGAAG GCGCTGGAAA GAGCCGGGAT GGCGATCACC GACATCGACC TGGTCGAGAT CAACGAGGCC TTCGCGGTGC AGGTCCTGGG CTCGGCGCGC GAGCTGGGCA TCGACGAGGA CAAGCTCAAC ATCTCAGGTG GCGCGATCGC CCTAGGTCAC CCGTTCGGTA TGACGGGCGC GCGCATCACC ACCACGCTGT TGAACAACCT GCAGACCTAC GACAAGACGT TCGGTCTGGA AACCATGTGT GTCGGTGGCG GCCAGGGCAT GGCGATGGTG ATCGAGCGCC TCGCG
Details for MytuD.00781.a.A1.GE45909
HARVESTED ON: 5/13/2025
SEQUENCED ON: data unavailable
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVST ARSPIGRAMK GSLVGMRPDD LAVQMVRAAL DKVPALNPHQ IDDLMMGCGL PGGESGFNIA RVVAVALGYD FLPGTTVNRY CSSSLQTTRM AFHAIKAGEG DAFISAGVET VSRFAKGNSD SWPDTKNPLF DGAQERSAAA AAGADEWHDP RTDQKLPDIY IAMGQTAENV AIMTGISREE QDRWGVRSQN RAEEAIKNGF FEREITPVTL PDGTTVSTDD GPRPGTTYEK VSELKPAFRP NGTVTAGNAC PLNDGAAAVV ITSDTKAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEASK KALERAGMAI TDIDLVEINE AFAVQVLGSA RELGIDEDKL NISGGAIALG HPFGMTGARI TTTLLNNLQT YDKTFGLETM CVGGGQGMAM VIERLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccagaagct gtcatcgtct cgactgcccg ctcgccgatt ggccgggcca tgaaaggatc gctggtcggc atgcggcccg acgacctggc cgtccagatg gtgcgcgccg cgctcgacaa ggttccggca ctgaaccccc atcagatcga cgacctcatg atgggctgtg gcctgccagg cggtgagtcc ggtttcaaca tcgcccgtgt cgtcgccgtc gcgctcggct atgacttcct gccgggcacc acggtcaacc ggtactgttc gtcgtcgctg cagaccaccc ggatggcctt ccacgcgatc aaggccggtg agggcgacgc gttcatctcc gcgggcgtgg aaaccgtctc ccggttcgcc aagggaaact ccgactcctg gccggacacc aagaacccgc tgttcgacgg ggctcaggaa cgttcggccg cggcagccgc cggcgccgac gaatggcacg atccccgcac cgatcagaag ctgccggaca tctatattgc gatgggccag accgcggaaa acgtcgccat catgaccggc atcagccgcg aagaacagga ccgctggggc gtgcgcagcc agaaccgggc cgaagaggcg atcaagaacg ggttcttcga acgagaaatc acgccggtca cccttccgga cggcaccacg gtcagcaccg acgacggccc gcgaccgggt accacctacg aaaaggtcag cgagctcaag ccggctttcc ggccgaatgg caccgtgacc gcggggaatg cctgtccgct caacgacggg gccgccgcgg tggtgatcac cagcgacacc aaggccaagg aattaggctt gacaccgctg gcgcgcattg tgtccaccgg ggtcagcggg ctgtccccgg agatcatggg cttagggccg atcgaggcgt ccaagaaggc gctggaaaga gccgggatgg cgatcaccga catcgacctg gtcgagatca acgaggcctt cgcggtgcag gtcctgggct cggcgcgcga gctgggcatc gacgaggaca agctcaacat ctcaggtggc gcgatcgccc taggtcaccc gttcggtatg acgggcgcgc gcatcaccac cacgctgttg aacaacctgc agacctacga caagacgttc ggtctggaaa ccatgtgtgt cggtggcggc cagggcatgg cgatggtgat cgagcgcctc gcgaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MytuD.00781.a.B1.GE27169
HARVESTED ON: 7/20/2009
SEQUENCED ON: 7/28/2009
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP EAVIVSTARS PIGRAMKGSL VGMRPDDLAV QMVRAALDKV PALNPHQIDD LMMGCGLPGG ESGFNIARVV AVALGYDFLP GTTVNRYCSS SLQTTRMAFH AIKAGEGGAF ISAGVETVSR FAKGNSDSWP DTKNPLFDGA QERSAAAAAG ADEWHDPRTD QKLPDIYIAM GQTAENVAIM TGISREEQDR WGVRSQNRAE EAIKNGFFER EITPVTLPDG TTVSTDDGPR PGTTYEKVSE LKPAFRPNGT VTAGNACPLN DGAAAVVITS DTKAKELGLT PLARIVSTGV SGLSPEIMGL GPIEASKKAL ERAGMAITDI DLVEINEAFA VQVLGSAREL GIDEDKLNIS GGAIALGHPF GMTGARITTT LLNNLQTYDK TFGLETMCVG GGQGMAMVIE RLA
Validated NT Sequence
atggctcacc accaccacca ccatatgcca gaagctgtca tcgtctcgac tgcccgctcg ccgattggcc gggccatgaa aggatcgctg gtcggcatgc ggcccgacga cctggccgtc cagatggtgc gcgccgcgct cgacaaggtt ccggcactga acccccatca gatcgacgac ctcatgatgg gctgtggcct gccaggcggt gagtccggtt tcaacatcgc ccgtgtcgtc gccgtcgcgc tcggctatga cttcctgccg ggcaccacgg tcaaccggta ctgttcgtcg tcgctgcaga ccacccggat ggccttccac gcgatcaagg ccggtgaggg cggcgcgttc atctccgcgg gcgtggaaac cgtctcccgg ttcgccaagg gaaactccga ctcctggccg gacaccaaga acccgctgtt cgacggggct caggaacgtt cggccgcggc agccgccggc gccgacgaat ggcacgatcc ccgcaccgat cagaagctgc cggacatcta tattgcgatg ggccagaccg cggaaaacgt cgccatcatg accggcatca gccgcgaaga acaggaccgc tggggcgtgc gcagccagaa ccgggccgaa gaggcgatca agaacgggtt cttcgaacga gaaatcacgc cggtcaccct tccggacggc accacggtca gcaccgacga cggcccgcga ccgggtacca cctacgaaaa ggtcagcgag ctcaagccgg ctttccggcc gaatggcacc gtgaccgcgg ggaatgcctg tccgctcaac gacggggccg ccgcggtggt gatcaccagc gacaccaagg ccaaggaatt aggcttgaca ccgctggcgc gcattgtgtc caccggggtc agcgggctgt ccccggagat catgggctta gggccgatcg aggcgtccaa gaaggcgctg gaaagagccg ggatggcgat caccgacatc gacctggtcg agatcaacga ggccttcgcg gtgcaggtcc tgggctcggc gcgcgagctg ggcatcgacg aggacaagct caacatctca ggtggcgcga tcgccctagg tcacccgttc ggtatgacgg gcgcgcgcat caccaccacg ctgttgaaca acctgcagac ctacgacaag acgttcggtc tggaaaccat gtgtgtcggt ggcggccagg gcatggcgat ggtgatcgag cgcctcgcgt aagtgagtaa gataggnncc ggcnnc
Expected Protein Sequence
MAHHHHHHMP EAVIVSTARS PIGRAMKGSL VGMRPDDLAV QMVRAALDKV PALNPHQIDD LMMGCGLPGG ESGFNIARVV AVALGYDFLP GTTVNRYCSS SLQTTRMAFH AIKAGEGDAF ISAGVETVSR FAKGNSDSWP DTKNPLFDGA QERSAAAAAG ADEWHDPRTD QKLPDIYIAM GQTAENVAIM TGISREEQDR WGVRSQNRAE EAIKNGFFER EITPVTLPDG TTVSTDDGPR PGTTYEKVSE LKPAFRPNGT VTAGNACPLN DGAAAVVITS DTKAKELGLT PLARIVSTGV SGLSPEIMGL GPIEASKKAL ERAGMAITDI DLVEINEAFA VQVLGSAREL GIDEDKLNIS GGAIALGHPF GMTGARITTT LLNNLQTYDK TFGLETMCVG GGQGMAMVIE RLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag aagctgtcat cgtctcgact gcccgctcgc cgattggccg ggccatgaaa ggatcgctgg tcggcatgcg gcccgacgac ctggccgtcc agatggtgcg cgccgcgctc gacaaggttc cggcactgaa cccccatcag atcgacgacc tcatgatggg ctgtggcctg ccaggcggtg agtccggttt caacatcgcc cgtgtcgtcg ccgtcgcgct cggctatgac ttcctgccgg gcaccacggt caaccggtac tgttcgtcgt cgctgcagac cacccggatg gccttccacg cgatcaaggc cggtgagggc gacgcgttca tctccgcggg cgtggaaacc gtctcccggt tcgccaaggg aaactccgac tcctggccgg acaccaagaa cccgctgttc gacggggctc aggaacgttc ggccgcggca gccgccggcg ccgacgaatg gcacgatccc cgcaccgatc agaagctgcc ggacatctat attgcgatgg gccagaccgc ggaaaacgtc gccatcatga ccggcatcag ccgcgaagaa caggaccgct ggggcgtgcg cagccagaac cgggccgaag aggcgatcaa gaacgggttc ttcgaacgag aaatcacgcc ggtcaccctt ccggacggca ccacggtcag caccgacgac ggcccgcgac cgggtaccac ctacgaaaag gtcagcgagc tcaagccggc tttccggccg aatggcaccg tgaccgcggg gaatgcctgt ccgctcaacg acggggccgc cgcggtggtg atcaccagcg acaccaaggc caaggaatta ggcttgacac cgctggcgcg cattgtgtcc accggggtca gcgggctgtc cccggagatc atgggcttag ggccgatcga ggcgtccaag aaggcgctgg aaagagccgg gatggcgatc accgacatcg acctggtcga gatcaacgag gccttcgcgg tgcaggtcct gggctcggcg cgcgagctgg gcatcgacga ggacaagctc aacatctcag gtggcgcgat cgccctaggt cacccgttcg gtatgacggg cgcgcgcatc accaccacgc tgttgaacaa cctgcagacc tacgacaaga cgttcggtct ggaaaccatg tgtgtcggtg gcggccaggg catggcgatg gtgatcgagc gcctcgcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MytuD.00781.a.B1.GE27590
HARVESTED ON: 8/17/2009
SEQUENCED ON: 9/29/2009
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP EAVIVSTARS PIGRAMKGSL VGMRPDDLAV QMVRAALDKV PALNPHQIDD LMMGCGLPGG ESGFNIARVV AVALGYDFLP GTTVNRYCSS SLQTTRMAFH AIKAGEGGAF ISAGVETVSR FAKGNSDSWP DTKNPLFDGA QERSAAAAAG ADEWHDPRTD QKLPDIYIAM GQTAENVAIM TGISREEQDR WGVRSQNRAE EAIKNGFFER EITPVTLPDG TTVSTDDGPR PGTTYEKVSE LKPAFRPNGT VTAGNACPLN DGAAAVVITS DTKAKELGLT PLARIVSTGV SGLSPEIMGL GPIEASKKAL ERAGMAITDI DLVEINEAFA VQVLGSAREL GIDEDKLNIS GGAIALGHPF GMTGARITTT LLNNLQTYDK TFGLETMCVG GGQGMAMVIE RLA
Validated NT Sequence
atggctcacc accaccacca ccatatgcca gaagctgtca tcgtctcgac tgcccgctcg ccgattggcc gggccatgaa aggatcgctg gtcggcatgc ggcccgacga cctggccgtc cagatggtgc gcgccgcgct cgacaaggtt ccggcactga acccccatca gatcgacgac ctcatgatgg gctgtggcct gccaggcggt gagtccggtt tcaacatcgc ccgtgtcgtc gccgtcgcgc tcggctatga cttcctgccg ggcaccacgg tcaaccggta ctgttcgtcg tcgctgcaga ccacccggat ggccttccac gcgatcaagg ccggtgaggg cggcgcgttc atctccgcgg gcgtggaaac cgtctcccgg ttcgccaagg gaaactccga ctcctggccg gacaccaaga acccgctgtt cgacggggct caggaacgtt cggccgcggc agccgccggc gccgacgaat ggcacgatcc ccgcaccgat cagaagctgc cggacatcta tattgcgatg ggccagaccg cggaaaacgt cgccatcatg accggcatca gccgcgaaga acaggaccgc tggggcgtgc gcagccagaa ccgggccgaa gaggcgatca agaacgggtt cttcgaacga gaaatcacgc cggtcaccct tccggacggc accacggtca gcaccgacga cggcccgcga ccgggtacca cctacgaaaa ggtcagcgag ctcaagccgg ctttccggcc gaatggcacc gtgaccgcgg ggaatgcctg tccgctcaac gacggggccg ccgcggtggt gatcaccagc gacaccaagg ccaaggaatt aggcttgaca ccgctggcgc gcattgtgtc caccggggtc agcgggctgt ccccggagat catgggctta gggccgatcg aggcgtccaa gaaggcgctg gaaagagccg ggatggcgat caccgacatc gacctggtcg agatcaacga ggccttcgcg gtgcaggtcc tgggctcggc gcgcgagctg ggcatcgacg aggacaagct caacatctca ggtggcgcga tcgccctagg tcacccgttc ggtatgacgg gcgcgcgcat caccaccacg ctgttgaaca acctgcagac ctacgacaag acgttcggtc tggaaaccat gtgtgtcggt ggcggccagg gcatggcgat ggtgatcgag cgcctcgcgt aagtgagtaa gataggnncc ggcnnc
Expected Protein Sequence
MAHHHHHHMP EAVIVSTARS PIGRAMKGSL VGMRPDDLAV QMVRAALDKV PALNPHQIDD LMMGCGLPGG ESGFNIARVV AVALGYDFLP GTTVNRYCSS SLQTTRMAFH AIKAGEGDAF ISAGVETVSR FAKGNSDSWP DTKNPLFDGA QERSAAAAAG ADEWHDPRTD QKLPDIYIAM GQTAENVAIM TGISREEQDR WGVRSQNRAE EAIKNGFFER EITPVTLPDG TTVSTDDGPR PGTTYEKVSE LKPAFRPNGT VTAGNACPLN DGAAAVVITS DTKAKELGLT PLARIVSTGV SGLSPEIMGL GPIEASKKAL ERAGMAITDI DLVEINEAFA VQVLGSAREL GIDEDKLNIS GGAIALGHPF GMTGARITTT LLNNLQTYDK TFGLETMCVG GGQGMAMVIE RLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccag aagctgtcat cgtctcgact gcccgctcgc cgattggccg ggccatgaaa ggatcgctgg tcggcatgcg gcccgacgac ctggccgtcc agatggtgcg cgccgcgctc gacaaggttc cggcactgaa cccccatcag atcgacgacc tcatgatggg ctgtggcctg ccaggcggtg agtccggttt caacatcgcc cgtgtcgtcg ccgtcgcgct cggctatgac ttcctgccgg gcaccacggt caaccggtac tgttcgtcgt cgctgcagac cacccggatg gccttccacg cgatcaaggc cggtgagggc gacgcgttca tctccgcggg cgtggaaacc gtctcccggt tcgccaaggg aaactccgac tcctggccgg acaccaagaa cccgctgttc gacggggctc aggaacgttc ggccgcggca gccgccggcg ccgacgaatg gcacgatccc cgcaccgatc agaagctgcc ggacatctat attgcgatgg gccagaccgc ggaaaacgtc gccatcatga ccggcatcag ccgcgaagaa caggaccgct ggggcgtgcg cagccagaac cgggccgaag aggcgatcaa gaacgggttc ttcgaacgag aaatcacgcc ggtcaccctt ccggacggca ccacggtcag caccgacgac ggcccgcgac cgggtaccac ctacgaaaag gtcagcgagc tcaagccggc tttccggccg aatggcaccg tgaccgcggg gaatgcctgt ccgctcaacg acggggccgc cgcggtggtg atcaccagcg acaccaaggc caaggaatta ggcttgacac cgctggcgcg cattgtgtcc accggggtca gcgggctgtc cccggagatc atgggcttag ggccgatcga ggcgtccaag aaggcgctgg aaagagccgg gatggcgatc accgacatcg acctggtcga gatcaacgag gccttcgcgg tgcaggtcct gggctcggcg cgcgagctgg gcatcgacga ggacaagctc aacatctcag gtggcgcgat cgccctaggt cacccgttcg gtatgacggg cgcgcgcatc accaccacgc tgttgaacaa cctgcagacc tacgacaaga cgttcggtct ggaaaccatg tgtgtcggtg gcggccaggg catggcgatg gtgatcgagc gcctcgcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MytuD.00781.a.HZ1.GE45910
HARVESTED ON: 5/13/2025
SEQUENCED ON: data unavailable
EXPECTED MW: 56kDa
OBSERVED MW: 56kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMP EAVIVSTARS PIGRAMKGSL VGMRPDDLAV QMVRAALDKV PALNPHQIDD LMMGCGLPGG ESGFNIARVV AVALGYDFLP GTTVNRYCSS SLQTTRMAFH AIKAGEGDAF ISAGVETVSR FAKGNSDSWP DTKNPLFDGA QERSAAAAAG ADEWHDPRTD QKLPDIYIAM GQTAENVAIM TGISREEQDR WGVRSQNRAE EAIKNGFFER EITPVTLPDG TTVSTDDGPR PGTTYEKVSE LKPAFRPNGT VTAGNACPLN DGAAAVVITS DTKAKELGLT PLARIVSTGV SGLSPEIMGL GPIEASKKAL ERAGMAITDI DLVEINEAFA VQVLGSAREL GIDEDKLNIS GGAIALGHPF GMTGARITTT LLNNLQTYDK TFGLETMCVG GGQGMAMVIE RLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgccagaagc tgtcatcgtc tcgactgccc gctcgccgat tggccgggcc atgaaaggat cgctggtcgg catgcggccc gacgacctgg ccgtccagat ggtgcgcgcc gcgctcgaca aggttccggc actgaacccc catcagatcg acgacctcat gatgggctgt ggcctgccag gcggtgagtc cggtttcaac atcgcccgtg tcgtcgccgt cgcgctcggc tatgacttcc tgccgggcac cacggtcaac cggtactgtt cgtcgtcgct gcagaccacc cggatggcct tccacgcgat caaggccggt gagggcgacg cgttcatctc cgcgggcgtg gaaaccgtct cccggttcgc caagggaaac tccgactcct ggccggacac caagaacccg ctgttcgacg gggctcagga acgttcggcc gcggcagccg ccggcgccga cgaatggcac gatccccgca ccgatcagaa gctgccggac atctatattg cgatgggcca gaccgcggaa aacgtcgcca tcatgaccgg catcagccgc gaagaacagg accgctgggg cgtgcgcagc cagaaccggg ccgaagaggc gatcaagaac gggttcttcg aacgagaaat cacgccggtc acccttccgg acggcaccac ggtcagcacc gacgacggcc cgcgaccggg taccacctac gaaaaggtca gcgagctcaa gccggctttc cggccgaatg gcaccgtgac cgcggggaat gcctgtccgc tcaacgacgg ggccgccgcg gtggtgatca ccagcgacac caaggccaag gaattaggct tgacaccgct ggcgcgcatt gtgtccaccg gggtcagcgg gctgtccccg gagatcatgg gcttagggcc gatcgaggcg tccaagaagg cgctggaaag agccgggatg gcgatcaccg acatcgacct ggtcgagatc aacgaggcct tcgcggtgca ggtcctgggc tcggcgcgcg agctgggcat cgacgaggac aagctcaaca tctcaggtgg cgcgatcgcc ctaggtcacc cgttcggtat gacgggcgcg cgcatcacca ccacgctgtt gaacaacctg cagacctacg acaagacgtt cggtctggaa accatgtgtg tcggtggcgg ccagggcatg gcgatggtga tcgagcgcct cgcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa