MyulA.00781.b

Beta-ketoacyl CoA thiolase

CENTER ID: MyulA.00781.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00781.b.A1.GE29739 Full length( MyulA.00781.b ) 1 399
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.492
RefSeq: YP_904550.1
UniProt: A0PL60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCGRLLASAP PKWRKPVPEA VIVAALRTPI GTARKGTLRD TNAFDLAHHV VSEATADLDP GQIDDVILGE GLYGGGVIAR HAAITAGLGH VPGLAQNRHC AAGQAAVQGA AASVRAGMDQ LIIAGGVNSA STSPRPRMQI DGEWVDWFPP THPDRPDAPN MDMSITVGWN AAVAAGVSRA EMDAWALRSH RNAIAAIDEG RFKEEIVPIP TPHGLFCVDE HPRRDTTMEK LAALKPLHPE IDGFSITAGN ACGANDGAAV LTIASDRMAQ GLGMPALARV LSWASVGVDP AATGLAPVEA IPKALARGHL SISDVDLFEI NEAFASMCVA TVKLLELDAD RVNVSGSGCS LGHPVAATGA RMLVTLVHEL RRRGGGIGLA AMCAGGGMGS ATVIEVAAP
NT Sequence
ATGTGCGGTC GGCTCCTGGC CTCCGCGCCG CCGAAGTGGA GAAAGCCCGT GCCTGAAGCC GTCATCGTCG CTGCCCTGCG CACCCCGATC GGAACCGCCC GCAAGGGAAC CTTGCGCGAC ACCAACGCAT TCGACCTGGC CCACCATGTG GTCAGTGAGG CCACCGCGGA TCTGGATCCG GGGCAGATCG ACGACGTAAT CCTGGGCGAA GGTCTCTACG GCGGCGGCGT CATCGCCCGC CACGCGGCCA TCACGGCGGG GCTTGGTCAT GTGCCCGGTC TTGCCCAGAA TCGGCACTGT GCCGCCGGAC AGGCGGCGGT GCAGGGCGCG GCCGCAAGCG TACGGGCGGG GATGGACCAG CTCATCATCG CCGGAGGCGT GAACTCGGCC TCCACCTCGC CGCGGCCCCG GATGCAGATC GATGGCGAGT GGGTCGACTG GTTCCCGCCG ACTCACCCGG ACCGGCCGGA TGCGCCGAAC ATGGACATGT CGATCACGGT CGGCTGGAAT GCGGCGGTGG CCGCCGGAGT GAGTCGCGCC GAGATGGACG CCTGGGCCCT GCGCTCGCAT CGCAACGCGA TCGCCGCGAT CGACGAGGGC CGGTTCAAGG AGGAGATTGT TCCGATCCCG ACGCCGCACG GCCTGTTCTG TGTCGACGAA CATCCGCGCC GGGACACGAC GATGGAGAAA CTGGCCGCGC TCAAGCCGCT GCATCCCGAG ATCGACGGCT TCTCGATCAC CGCCGGCAAC GCCTGCGGCG CCAATGACGG CGCCGCGGTG CTGACCATCG CAAGCGACCG GATGGCCCAG GGGTTGGGCA TGCCGGCGCT GGCGCGGGTG CTCAGCTGGG CGTCGGTGGG TGTCGATCCG GCCGCCACCG GCCTGGCCCC GGTCGAAGCT ATTCCGAAAG CGCTTGCACG GGGCCACCTA TCAATCTCCG ACGTTGATCT GTTCGAGATC AACGAAGCGT TCGCGTCAAT GTGCGTGGCG ACCGTCAAAC TGCTGGAACT GGACGCCGAT CGGGTCAACG TGAGCGGCAG CGGATGCTCG TTGGGGCACC CGGTGGCCGC GACCGGGGCC AGGATGCTGG TGACGCTGGT GCACGAACTG CGCCGACGCG GCGGCGGGAT TGGGCTGGCG GCGATGTGCG CCGGTGGCGG CATGGGATCG GCGACGGTCA TCGAGGTTGC GGCACCA
Details for MyulA.00781.b.A1.GE29739
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 43kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 89
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMCGRLLASA PPKWRKPVPE AVIVAALRTP IGTARKGTLR DTNAFDLAHH VVSEATADLD PGQIDDVILG EGLYGGGVIA RHAAITAGLG HVPGLAQNRH CAAGQAAVQG AAASVRAGMD QLIIAGGVNS ASTSPRPRMQ IDGEWVDWFP PTHPDRPDAP NMDMSITVGW NAAVAAGVSR AEMDAWALRS HRNAIAAIDE GRFKEEIVPI PTPHGLFCVD EHPRRDTTME KLAALKPLHP EIDGFSITAG NACGANDGAA XLXIASDRMA XGWACRRWRX AQLGVGGXRS XXPPXWPGRS XXXXAXXGXL XXXTXIXXXX TXXXXXXXXX XXXXXXXXRX XXXXXGXXXX XXXXXX
Validated NT Sequence
ttttgtttac tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtgcgg tcggctcctg gcctccgcgc cgccgaagtg gagaaagccc gtgcctgaag ccgtcatcgt cgctgccctg cgcaccccga tcggaaccgc ccgcaaggga accttgcgcg acaccaacgc attcgacctg gcccaccatg tggtcagtga ggccaccgcg gatctggatc cggggcagat cgacgacgta atcctgggcg aaggtctcta cggcggcggc gtcatcgccc gccacgcggc catcacggcg gggcttggtc atgtgcccgg tcttgcccag aatcggcact gtgccgccgg acaggcggcg gtgcagggcg cggccgcaag cgtacgggcg gggatggacc agctcatcat cgccggaggc gtgaactcgg cctccacctc gccgcggccc cggatgcaga tcgatggcga gtgggtcgac tggttcccgc cgactcaccc ggaccggccg gatgcgccga acatggacat gtcgatcacg gtcggctgga atgcggcggt ggccgccgga gtgagtcgcg ccgagatgga cgcctgggcc ctgcgctcgc atcgcaacgc gatcgccgcg atcgacgagg gccggttcaa ggaggagatt gttccgatcc cgacgccgca cggcctgttc tgtgtcgacg aacatccgcg ccgggacacg acgatggaga aactggccgc gctcaagccg ctgcatcccg agatcgacgg cttctcgatc accgccggca acgcctgcgg cgccaatgac ggcgccgcgg ngctgancat cgcaagcgac cggatggccc nnggttgggc atgccggcgc tggcgcngng ctcagctggg ngtcggtggg ngtcgatccn gnncgccncc ggnctggccc ggtcgaagct nnnnnnaaan cgctnnnnng gggnnnctat nannnncgac ntngatctnn nnnanatnna cgnannnnnc nnntnnnnng nnngnnannn nnannnnntn nnnnnnnnnn nancgnnnnn ncnnngnnnn nnggggnnnn nnnnnnnnnn nnnnnnnnan gnnncnnct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCGRLLASA PPKWRKPVPE AVIVAALRTP IGTARKGTLR DTNAFDLAHH VVSEATADLD PGQIDDVILG EGLYGGGVIA RHAAITAGLG HVPGLAQNRH CAAGQAAVQG AAASVRAGMD QLIIAGGVNS ASTSPRPRMQ IDGEWVDWFP PTHPDRPDAP NMDMSITVGW NAAVAAGVSR AEMDAWALRS HRNAIAAIDE GRFKEEIVPI PTPHGLFCVD EHPRRDTTME KLAALKPLHP EIDGFSITAG NACGANDGAA VLTIASDRMA QGLGMPALAR VLSWASVGVD PAATGLAPVE AIPKALARGH LSISDVDLFE INEAFASMCV ATVKLLELDA DRVNVSGSGC SLGHPVAATG ARMLVTLVHE LRRRGGGIGL AAMCAGGGMG SATVIEVAAP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcggtcgg ctcctggcct ccgcgccgcc gaagtggaga aagcccgtgc ctgaagccgt catcgtcgct gccctgcgca ccccgatcgg aaccgcccgc aagggaacct tgcgcgacac caacgcattc gacctggccc accatgtggt cagtgaggcc accgcggatc tggatccggg gcagatcgac gacgtaatcc tgggcgaagg tctctacggc ggcggcgtca tcgcccgcca cgcggccatc acggcggggc ttggtcatgt gcccggtctt gcccagaatc ggcactgtgc cgccggacag gcggcggtgc agggcgcggc cgcaagcgta cgggcgggga tggaccagct catcatcgcc ggaggcgtga actcggcctc cacctcgccg cggccccgga tgcagatcga tggcgagtgg gtcgactggt tcccgccgac tcacccggac cggccggatg cgccgaacat ggacatgtcg atcacggtcg gctggaatgc ggcggtggcc gccggagtga gtcgcgccga gatggacgcc tgggccctgc gctcgcatcg caacgcgatc gccgcgatcg acgagggccg gttcaaggag gagattgttc cgatcccgac gccgcacggc ctgttctgtg tcgacgaaca tccgcgccgg gacacgacga tggagaaact ggccgcgctc aagccgctgc atcccgagat cgacggcttc tcgatcaccg ccggcaacgc ctgcggcgcc aatgacggcg ccgcggtgct gaccatcgca agcgaccgga tggcccaggg gttgggcatg ccggcgctgg cgcgggtgct cagctgggcg tcggtgggtg tcgatccggc cgccaccggc ctggccccgg tcgaagctat tccgaaagcg cttgcacggg gccacctatc aatctccgac gttgatctgt tcgagatcaa cgaagcgttc gcgtcaatgt gcgtggcgac cgtcaaactg ctggaactgg acgccgatcg ggtcaacgtg agcggcagcg gatgctcgtt ggggcacccg gtggccgcga ccggggccag gatgctggtg acgctggtgc acgaactgcg ccgacgcggc ggcgggattg ggctggcggc gatgtgcgcc ggtggcggca tgggatcggc gacggtcatc gaggttgcgg caccaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa