SSGCID: Target Page

MyavA.00781.b

Acetyl-CoA acetyltransferase

CENTER ID:	MyavA.00781.b
ORGANISM:	Mycobacterium avium 104
ASSOCIATED DISEASE:	Johne's disease, paratuberculosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyavA.00781.b.A1.GE29022	Full length( MyavA.00781.b )		1	405

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|243243.7.peg.1171
RefSeq:	YP_880444.1
UniProt:	A0A0H2ZT78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPEAVIVSTA RSPIGRAMKG SLVSMRPDDL AVQIVRAALD KVPALNPHQI DDLILGCGQP GGESGFNLAR VVAVELGFDF LPGTTVNRYC SSSLQTSRMA FHAIKAGEGD AFISAGVETV SRFPKGSADS WPDTKNPLFS EAQERSAAAA EGAEEWHDPR ADGKLPDIYI AMGQTAENVA LLTGISREDQ DHWGVRSQNR AEEAIKSGFF EREITPVTLP DGTTVSTDDG PRPGTTYEKI RELKPVFRPN GTVTAGNACP LNDGAAAVVI TSDTKAKELG LTPLARIVST GVSGLSPEIM GLGPIEATKK ALARAKMSVS DIDLFEINEA FAVQVLGSAR ELGIDEDKLN VSGGAIALGH PFGMTGARIT TTLLNNLQTH DKTFGLETMC VGGGQGMAMV IERLS

NT Sequence

ATGCCTGAAG CCGTCATCGT CTCAACTGCT CGCTCGCCGA TCGGCCGCGC GATGAAGGGA TCGCTGGTCA GCATGCGCCC CGACGACCTG GCCGTGCAGA TCGTGCGCGC CGCGCTGGAC AAGGTGCCGG CGTTGAACCC GCACCAGATC GACGACCTGA TCCTGGGCTG CGGCCAGCCC GGCGGTGAGT CCGGATTCAA CCTGGCCCGG GTGGTCGCCG TCGAGCTGGG CTTCGACTTC CTGCCGGGCA CCACCGTCAA CCGGTACTGC TCCTCGTCGC TGCAGACCTC GCGGATGGCG TTCCACGCGA TCAAGGCCGG CGAGGGCGAC GCGTTCATCT CCGCGGGGGT GGAGACGGTA TCCCGGTTCC CCAAGGGCAG CGCCGACTCG TGGCCGGACA CCAAGAACCC GCTGTTCAGC GAGGCGCAGG AGCGGTCGGC CGCCGCGGCC GAGGGCGCCG AGGAGTGGCA CGACCCGCGC GCCGACGGCA AGCTGCCCGA CATCTACATC GCGATGGGGC AGACCGCCGA GAACGTCGCC CTGCTGACCG GCATCAGCCG CGAGGACCAG GACCACTGGG GGGTGCGCAG CCAGAACCGC GCCGAGGAAG CGATCAAGAG CGGGTTCTTC GAGCGGGAGA TCACCCCGGT CACGCTGCCG GACGGCACCA CGGTCAGCAC CGACGACGGC CCACGCCCCG GCACCACGTA CGAGAAGATC CGCGAGCTCA AGCCGGTGTT CCGGCCCAAC GGCACGGTGA CCGCGGGCAA CGCCTGCCCG CTGAACGACG GCGCCGCGGC GGTGGTGATC ACCAGCGACA CCAAGGCCAA GGAGCTCGGC CTGACGCCGC TGGCGCGCAT CGTGTCCACC GGGGTCAGCG GCCTGTCGCC GGAGATCATG GGCCTGGGCC CGATCGAGGC CACCAAGAAG GCGCTGGCCC GCGCCAAAAT GTCGGTCAGC GACATCGACC TGTTCGAGAT CAACGAGGCG TTCGCGGTCC AGGTGCTGGG CTCGGCCCGC GAGCTGGGCA TCGACGAGGA CAAGCTGAAC GTGTCCGGCG GCGCGATCGC GCTGGGTCAT CCGTTCGGCA TGACCGGCGC CCGGATCACC ACCACGCTGC TCAACAACCT GCAGACCCAC GACAAGACGT TCGGCCTGGA GACCATGTGC GTCGGCGGCG GCCAGGGCAT GGCGATGGTG ATCGAGCGCC TCAGC

Details for MyavA.00781.b.A1.GE29022

HARVESTED ON:	4/9/2010
SEQUENCED ON:	4/29/2010
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

Validated NT Sequence

tagctgaggc gctcgatcac catcgccatg ccctggccgc cgccgacgca catggtctcc aggccgaacg tcttgtcgtg ggtctgcagg ttgttgagca gcgtggtggt gatccgggcg ccggtcatgc cgaacggatg acccagcgcg atcgctccgc cggacacgtt cagtttgtcc tcgtcgatgc ccagctcgcg ggccgagccc agcacctgga ccgcgaacgc ctcgttgatc tcgaacaggt cgatgtcgct gaccgacatc ttggcgcggg ccagcgcctt tttggtggcc tcgatcgggc ccaggcccat gatctccggc gacaggccgc tgaccccggt ggacacgatg cgcgccagcg gcgtcaggcc gagctccttg gccttggtgt cgctggtgat caccaccgcc gcggcgccgt cgttcagcgg gcaggcgttg cccgcggtca ccgtgccgtt gggccggaac accggcttga gctcgcggat cttctcgtac gtggtgccgg ggcgtgggcc gtcgtcggtg ctgaccgtgg tgccgtccgg cagcgtgacc ggggtgatct cccgctcgaa gaacccgctc ttgatcgctt cctcggcgcg gttctggctg cgcacccccc agtggtcctg gtcctcgcgg ctgatgccgg tcagcagggc gacgttctcg gcggtctgcc ccatcgcgat gtagatatcg ggcagcttgc cgtcggcgcg cgggtcgtgc cactcctcgg cgccctcggc cgcggcggcc gaccgctcct gcgcctcgct gaacagcggg ttcttggtgt ccggccacga gtcggcgctg cccttgggga accgggatac cgtctccacc cccgcggaga tgaacgcgtc accctcgccg gccttgatcg cgtggaacgc catccgcgag gtctgcagcg acgaggagca gtaccggttg acggtggtgc ccggcaggaa gtcgaagccc agctcgacgg cgaccacccg ggccaggttg aatccggact caccgccggg ctggccgcag cccaggatca ggtcgtcgat ctggtgcggg ttcaacgccg gcaccttgtc cagcgcggcg cgcacgatct gcacggccag gtcgtcgggg cgcatgctga ccagcgatcc cttcatcgcg cggccgatcg gcgagcgagc agttgagacg atgacggctt caggcatcga accaggaccc tgggtctgag cttccagggt a

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPEAVIVST ARSPIGRAMK GSLVSMRPDD LAVQIVRAAL DKVPALNPHQ IDDLILGCGQ PGGESGFNLA RVVAVELGFD FLPGTTVNRY CSSSLQTSRM AFHAIKAGEG DAFISAGVET VSRFPKGSAD SWPDTKNPLF SEAQERSAAA AEGAEEWHDP RADGKLPDIY IAMGQTAENV ALLTGISRED QDHWGVRSQN RAEEAIKSGF FEREITPVTL PDGTTVSTDD GPRPGTTYEK IRELKPVFRP NGTVTAGNAC PLNDGAAAVV ITSDTKAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEATK KALARAKMSV SDIDLFEINE AFAVQVLGSA RELGIDEDKL NVSGGAIALG HPFGMTGARI TTTLLNNLQT HDKTFGLETM CVGGGQGMAM VIERLS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgaagcc gtcatcgtct caactgctcg ctcgccgatc ggccgcgcga tgaagggatc gctggtcagc atgcgccccg acgacctggc cgtgcagatc gtgcgcgccg cgctggacaa ggtgccggcg ttgaacccgc accagatcga cgacctgatc ctgggctgcg gccagcccgg cggtgagtcc ggattcaacc tggcccgggt ggtcgccgtc gagctgggct tcgacttcct gccgggcacc accgtcaacc ggtactgctc ctcgtcgctg cagacctcgc ggatggcgtt ccacgcgatc aaggccggcg agggcgacgc gttcatctcc gcgggggtgg agacggtatc ccggttcccc aagggcagcg ccgactcgtg gccggacacc aagaacccgc tgttcagcga ggcgcaggag cggtcggccg ccgcggccga gggcgccgag gagtggcacg acccgcgcgc cgacggcaag ctgcccgaca tctacatcgc gatggggcag accgccgaga acgtcgccct gctgaccggc atcagccgcg aggaccagga ccactggggg gtgcgcagcc agaaccgcgc cgaggaagcg atcaagagcg ggttcttcga gcgggagatc accccggtca cgctgccgga cggcaccacg gtcagcaccg acgacggccc acgccccggc accacgtacg agaagatccg cgagctcaag ccggtgttcc ggcccaacgg cacggtgacc gcgggcaacg cctgcccgct gaacgacggc gccgcggcgg tggtgatcac cagcgacacc aaggccaagg agctcggcct gacgccgctg gcgcgcatcg tgtccaccgg ggtcagcggc ctgtcgccgg agatcatggg cctgggcccg atcgaggcca ccaagaaggc gctggcccgc gccaaaatgt cggtcagcga catcgacctg ttcgagatca acgaggcgtt cgcggtccag gtgctgggct cggcccgcga gctgggcatc gacgaggaca agctgaacgt gtccggcggc gcgatcgcgc tgggtcatcc gttcggcatg accggcgccc ggatcaccac cacgctgctc aacaacctgc agacccacga caagacgttc ggcctggaga ccatgtgcgt cggcggcggc cagggcatgg cgatggtgat cgagcgcctc agcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa