MyabA.00781.b

Probable acyl-CoA thiolase

CENTER ID: MyabA.00781.b
ORGANISM: Mycobacterium abscessus ATCC 19977 / DSM 44196
ASSOCIATED DISEASE: Skin abscess
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00781.b.A1.GE30346 Full length( MyabA.00781.b ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyabA.00781.b.A1.PS00994 Full length( MyabA.00781.b ) 1 405

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|36809.5.peg.1230
RefSeq: YP_001701934.1
UniProt: B1MKI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEAVIVSTA RSPIGRAGKG SLVSMRPDDL AAQMVRAALD KVPALDPKDV DDLMLGCGQP GGEAGFNIGR AVAVQLGYDF LPGTTVNRYC SSSLQTTRMA FHAIKAGEGH AFISAGVETV SRFPKGTSDG WPDSHNPLYA DAEARTATAA QGATEWHDPR NDGLTPDVYI AMGQTAENVA LHTGISREDQ DHWGVRSQNR AEKAIADGFF EREITPVTLA DGTVVAKDDG PRAGTTYEAI SQLKPVFRPN GTITAGNACP LNDGAAALVV MSDVRAKELG LTPLARIVST GVSGLSPEIM GLGPIEAVKR ALANAGKSIG DIDLFEINEA FAVQVLGSAR ALGIDEDKLN VSGGAIALGH PFGMTGARIT ATLLNNLTTY DKTFGVETMC VGGGQGMAMV IERLA
NT Sequence
ATGCCCGAAG CCGTGATCGT TTCCACTGCC CGCTCCCCGA TCGGGCGAGC CGGCAAGGGC TCGCTGGTGA GCATGCGGCC GGATGACCTG GCCGCGCAGA TGGTGCGCGC CGCCCTCGAC AAGGTGCCCG CACTGGACCC CAAGGATGTC GACGACCTGA TGCTGGGCTG CGGTCAGCCA GGCGGCGAGG CCGGTTTCAA CATCGGTCGC GCGGTGGCGG TACAGCTGGG CTACGACTTC CTGCCCGGCA CCACCGTCAA CCGTTACTGC TCGTCGTCGC TGCAGACCAC CCGCATGGCG TTCCACGCCA TCAAGGCCGG CGAGGGTCAC GCATTCATTT CCGCTGGTGT GGAGACGGTT TCGCGCTTTC CGAAGGGCAC TTCGGACGGA TGGCCGGACA GCCACAACCC GCTGTACGCC GACGCCGAGG CCCGCACCGC GACCGCGGCA CAGGGTGCCA CCGAATGGCA CGACCCGCGC AACGACGGCC TGACCCCCGA CGTGTACATC GCGATGGGCC AGACCGCCGA GAACGTGGCA CTGCACACCG GCATCAGCCG TGAGGACCAG GACCACTGGG GCGTGCGCAG CCAGAACCGG GCCGAAAAGG CCATTGCCGA TGGCTTTTTC GAGCGCGAGA TCACCCCGGT GACGCTGGCC GACGGTACCG TCGTGGCCAA GGACGACGGC CCCCGCGCCG GCACCACCTA CGAGGCCATC AGCCAGCTGA AGCCGGTGTT CCGCCCCAAC GGCACCATCA CTGCCGGTAA CGCCTGCCCG CTGAACGACG GTGCGGCCGC GCTGGTCGTG ATGAGCGATG TGCGCGCCAA GGAGCTGGGC CTGACCCCGC TGGCGCGCAT CGTCTCTACC GGTGTGTCCG GCCTGTCGCC GGAAATCATG GGCCTGGGCC CGATCGAGGC CGTCAAGCGC GCACTGGCCA ACGCGGGCAA GTCGATTGGC GACATCGATC TGTTCGAGAT CAACGAGGCG TTCGCCGTCC AGGTGCTGGG CTCGGCGCGC GCGCTCGGCA TCGATGAGGA CAAGCTGAAC GTCTCCGGCG GTGCGATCGC GCTGGGTCAC CCCTTCGGCA TGACCGGTGC CCGCATCACC GCCACCCTGC TGAACAACCT GACCACCTAC GACAAGACCT TCGGTGTCGA GACCATGTGT GTGGGTGGCG GCCAGGGTAT GGCGATGGTC ATCGAGCGGC TCGCC
Details for MyabA.00781.b.A1.GE30346
HARVESTED ON: 9/8/2010
SEQUENCED ON: 9/17/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MPEAVIVSTA RSPIGRAGKG SLVSMRPDDL AAQMVRAALD KVPALDPKDV DDLMLGCGQP GGEAGFNIGR AVAVQLGYDF LPGTTVNRYC SSSLQTTRMA FHAIKAGEGH AFISAGVETV SRFPKGTSDG WPDSHNPLYA DAEARTATAA QGATEWHDPR NDGLTPDVYI AMGQTAENVA LHTGISREDQ DHWGVRSQNR AEKAIADGFF EREITPVTLA DGTVVAKDDG PRAGTTYEAI SQLKPVFRPN GTITAGNACP LNDGAAALVV MSDVRAKELG LTPLARIVST GVSGLSPEIM GLGPIEAVKR ALANAGKSIG DIDLFEINEA FAVQVLGSAR ALGIDEDKLN VSGGAIALGH PFGMTGARIT ATLLNNLTTY DKTFGVETMC VGGGQGMAMV IERL
Validated NT Sequence
gcgagccgct cgatgaccat cgccataccc tggccgccac ccacacacat ggtctcgaca ccgaaggtct tgtcgtaggt ggtcaggttg ttcagcaggg tggcggtgat gcgggcaccg gtcatgccga aggggtgacc cagcgcgatc gcaccgccgg agacgttcag cttgtcctca tcgatgccga gcgcgcgcgc cgagcccagc acctggacgg cgaacgcctc gttgatctcg aacagatcga tgtcgccaat cgacttgccc gcgttggcca gtgcgcgctt gacggcctcg atcgggccca ggcccatgat ttccggcgac aggccggaca caccggtaga gacgatgcgc gccagcgggg tcaggcccag ctccttggcg cgcacatcgc tcatcacgac cagcgcggcc gcaccgtcgt tcagcgggca ggcgttaccg gcagtgatgg tgccgttggg gcggaacacc ggcttcagct ggctgatggc ctcgtaggtg gtgccggcgc gggggccgtc gtccttggcc acgacggtac cgtcggccag cgtcaccggg gtgatctcgc gctcgaaaaa gccatcggca atggcctttt cggcccggtt ctggctgcgc acgccccagt ggtcctggtc ctcacggctg atgccggtgt gcagtgccac gttctcggcg gtctggccca tcgcgatgta cacgtcgggg gtcaggccgt cgttgcgcgg gtcgtgccat tcggtggcac cctgtgccgc ggtcgcggtg cgggcctcgg cgtcggcgta cagcgggttg tggctgtccg gccatccgtc cgaagtgccc ttcggaaagc gcgaaaccgt ctccacacca gcggaaatga atgcgtgacc ctcgccggcc ttgatggcgt ggaacgccat gcgggtggtc tgcagcgacg acgagcagta acggttgacg gtggtgccgg gcaggaagtc gtagcccagc tgtaccgcca ccgcgcgacc gatgttgaaa ccggcctcgc cgcctggctg accgcagccc agcatcaggt cgtcgacatc cttggggtcc agtgcgggca ccttgtcgag ggcggcgcgc accatctgcg cggccaggtc atccggccgc atgctcacca gcgagccctt gccggctcgc ccgatcgggg agcgggcagt ggaaacgatc acggcttcgg gcatcgaacc aggaccctgg gtctgagctt ccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVST ARSPIGRAGK GSLVSMRPDD LAAQMVRAAL DKVPALDPKD VDDLMLGCGQ PGGEAGFNIG RAVAVQLGYD FLPGTTVNRY CSSSLQTTRM AFHAIKAGEG HAFISAGVET VSRFPKGTSD GWPDSHNPLY ADAEARTATA AQGATEWHDP RNDGLTPDVY IAMGQTAENV ALHTGISRED QDHWGVRSQN RAEKAIADGF FEREITPVTL ADGTVVAKDD GPRAGTTYEA ISQLKPVFRP NGTITAGNAC PLNDGAAALV VMSDVRAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEAVK RALANAGKSI GDIDLFEINE AFAVQVLGSA RALGIDEDKL NVSGGAIALG HPFGMTGARI TATLLNNLTT YDKTFGVETM CVGGGQGMAM VIERLA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccgaagcc gtgatcgttt ccactgcccg ctccccgatc gggcgagccg gcaagggctc gctggtgagc atgcggccgg atgacctggc cgcgcagatg gtgcgcgccg ccctcgacaa ggtgcccgca ctggacccca aggatgtcga cgacctgatg ctgggctgcg gtcagccagg cggcgaggcc ggtttcaaca tcggtcgcgc ggtggcggta cagctgggct acgacttcct gcccggcacc accgtcaacc gttactgctc gtcgtcgctg cagaccaccc gcatggcgtt ccacgccatc aaggccggcg agggtcacgc attcatttcc gctggtgtgg agacggtttc gcgctttccg aagggcactt cggacggatg gccggacagc cacaacccgc tgtacgccga cgccgaggcc cgcaccgcga ccgcggcaca gggtgccacc gaatggcacg acccgcgcaa cgacggcctg acccccgacg tgtacatcgc gatgggccag accgccgaga acgtggcact gcacaccggc atcagccgtg aggaccagga ccactggggc gtgcgcagcc agaaccgggc cgaaaaggcc attgccgatg gctttttcga gcgcgagatc accccggtga cgctggccga cggtaccgtc gtggccaagg acgacggccc ccgcgccggc accacctacg aggccatcag ccagctgaag ccggtgttcc gccccaacgg caccatcact gccggtaacg cctgcccgct gaacgacggt gcggccgcgc tggtcgtgat gagcgatgtg cgcgccaagg agctgggcct gaccccgctg gcgcgcatcg tctctaccgg tgtgtccggc ctgtcgccgg aaatcatggg cctgggcccg atcgaggccg tcaagcgcgc actggccaac gcgggcaagt cgattggcga catcgatctg ttcgagatca acgaggcgtt cgccgtccag gtgctgggct cggcgcgcgc gctcggcatc gatgaggaca agctgaacgt ctccggcggt gcgatcgcgc tgggtcaccc cttcggcatg accggtgccc gcatcaccgc caccctgctg aacaacctga ccacctacga caagaccttc ggtgtcgaga ccatgtgtgt gggtggcggc cagggtatgg cgatggtcat cgagcggctc gcctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MyabA.00781.b.A1.PS00994
PURIFICATION DATe: 3/7/2011
CONCENTRATION: 17.83mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 75µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MPEAVIVSTA RSPIGRAGKG SLVSMRPDDL AAQMVRAALD KVPALDPKDV DDLMLGCGQP GGEAGFNIGR AVAVQLGYDF LPGTTVNRYC SSSLQTTRMA FHAIKAGEGH AFISAGVETV SRFPKGTSDG WPDSHNPLYA DAEARTATAA QGATEWHDPR NDGLTPDVYI AMGQTAENVA LHTGISREDQ DHWGVRSQNR AEKAIADGFF EREITPVTLA DGTVVAKDDG PRAGTTYEAI SQLKPVFRPN GTITAGNACP LNDGAAALVV MSDVRAKELG LTPLARIVST GVSGLSPEIM GLGPIEAVKR ALANAGKSIG DIDLFEINEA FAVQVLGSAR ALGIDEDKLN VSGGAIALGH PFGMTGARIT ATLLNNLTTY DKTFGVETMC VGGGQGMAMV IERL
Validated NT Sequence
gcgagccgct cgatgaccat cgccataccc tggccgccac ccacacacat ggtctcgaca ccgaaggtct tgtcgtaggt ggtcaggttg ttcagcaggg tggcggtgat gcgggcaccg gtcatgccga aggggtgacc cagcgcgatc gcaccgccgg agacgttcag cttgtcctca tcgatgccga gcgcgcgcgc cgagcccagc acctggacgg cgaacgcctc gttgatctcg aacagatcga tgtcgccaat cgacttgccc gcgttggcca gtgcgcgctt gacggcctcg atcgggccca ggcccatgat ttccggcgac aggccggaca caccggtaga gacgatgcgc gccagcgggg tcaggcccag ctccttggcg cgcacatcgc tcatcacgac cagcgcggcc gcaccgtcgt tcagcgggca ggcgttaccg gcagtgatgg tgccgttggg gcggaacacc ggcttcagct ggctgatggc ctcgtaggtg gtgccggcgc gggggccgtc gtccttggcc acgacggtac cgtcggccag cgtcaccggg gtgatctcgc gctcgaaaaa gccatcggca atggcctttt cggcccggtt ctggctgcgc acgccccagt ggtcctggtc ctcacggctg atgccggtgt gcagtgccac gttctcggcg gtctggccca tcgcgatgta cacgtcgggg gtcaggccgt cgttgcgcgg gtcgtgccat tcggtggcac cctgtgccgc ggtcgcggtg cgggcctcgg cgtcggcgta cagcgggttg tggctgtccg gccatccgtc cgaagtgccc ttcggaaagc gcgaaaccgt ctccacacca gcggaaatga atgcgtgacc ctcgccggcc ttgatggcgt ggaacgccat gcgggtggtc tgcagcgacg acgagcagta acggttgacg gtggtgccgg gcaggaagtc gtagcccagc tgtaccgcca ccgcgcgacc gatgttgaaa ccggcctcgc cgcctggctg accgcagccc agcatcaggt cgtcgacatc cttggggtcc agtgcgggca ccttgtcgag ggcggcgcgc accatctgcg cggccaggtc atccggccgc atgctcacca gcgagccctt gccggctcgc ccgatcgggg agcgggcagt ggaaacgatc acggcttcgg gcatcgaacc aggaccctgg gtctgagctt ccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVST ARSPIGRAGK GSLVSMRPDD LAAQMVRAAL DKVPALDPKD VDDLMLGCGQ PGGEAGFNIG RAVAVQLGYD FLPGTTVNRY CSSSLQTTRM AFHAIKAGEG HAFISAGVET VSRFPKGTSD GWPDSHNPLY ADAEARTATA AQGATEWHDP RNDGLTPDVY IAMGQTAENV ALHTGISRED QDHWGVRSQN RAEKAIADGF FEREITPVTL ADGTVVAKDD GPRAGTTYEA ISQLKPVFRP NGTITAGNAC PLNDGAAALV VMSDVRAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEAVK RALANAGKSI GDIDLFEINE AFAVQVLGSA RALGIDEDKL NVSGGAIALG HPFGMTGARI TATLLNNLTT YDKTFGVETM CVGGGQGMAM VIERLA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCCGAAGCC GTGATCGTTT CCACTGCCCG CTCCCCGATC GGGCGAGCCG GCAAGGGCTC GCTGGTGAGC ATGCGGCCGG ATGACCTGGC CGCGCAGATG GTGCGCGCCG CCCTCGACAA GGTGCCCGCA CTGGACCCCA AGGATGTCGA CGACCTGATG CTGGGCTGCG GTCAGCCAGG CGGCGAGGCC GGTTTCAACA TCGGTCGCGC GGTGGCGGTA CAGCTGGGCT ACGACTTCCT GCCCGGCACC ACCGTCAACC GTTACTGCTC GTCGTCGCTG CAGACCACCC GCATGGCGTT CCACGCCATC AAGGCCGGCG AGGGTCACGC ATTCATTTCC GCTGGTGTGG AGACGGTTTC GCGCTTTCCG AAGGGCACTT CGGACGGATG GCCGGACAGC CACAACCCGC TGTACGCCGA CGCCGAGGCC CGCACCGCGA CCGCGGCACA GGGTGCCACC GAATGGCACG ACCCGCGCAA CGACGGCCTG ACCCCCGACG TGTACATCGC GATGGGCCAG ACCGCCGAGA ACGTGGCACT GCACACCGGC ATCAGCCGTG AGGACCAGGA CCACTGGGGC GTGCGCAGCC AGAACCGGGC CGAAAAGGCC ATTGCCGATG GCTTTTTCGA GCGCGAGATC ACCCCGGTGA CGCTGGCCGA CGGTACCGTC GTGGCCAAGG ACGACGGCCC CCGCGCCGGC ACCACCTACG AGGCCATCAG CCAGCTGAAG CCGGTGTTCC GCCCCAACGG CACCATCACT GCCGGTAACG CCTGCCCGCT GAACGACGGT GCGGCCGCGC TGGTCGTGAT GAGCGATGTG CGCGCCAAGG AGCTGGGCCT GACCCCGCTG GCGCGCATCG TCTCTACCGG TGTGTCCGGC CTGTCGCCGG AAATCATGGG CCTGGGCCCG ATCGAGGCCG TCAAGCGCGC ACTGGCCAAC GCGGGCAAGT CGATTGGCGA CATCGATCTG TTCGAGATCA ACGAGGCGTT CGCCGTCCAG GTGCTGGGCT CGGCGCGCGC GCTCGGCATC GATGAGGACA AGCTGAACGT CTCCGGCGGT GCGATCGCGC TGGGTCACCC CTTCGGCATG ACCGGTGCCC GCATCACCGC CACCCTGCTG AACAACCTGA CCACCTACGA CAAGACCTTC GGTGTCGAGA CCATGTGTGT GGGTGGCGGC CAGGGTATGG CGATGGTCAT CGAGCGGCTC GCCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA