MyulA.00781.a

Beta-ketoacyl CoA thiolase FadA3

CENTER ID: MyulA.00781.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00781.a.A1.GE29738 Full length( MyulA.00781.a ) 1 405
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.322
RefSeq: YP_904428.1
UniProt: A0PKT8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPEAVIVSTA RSPIGRAMKA SLVSMRPDDL AVQMVRAALD KVPALNPHQI DDLIMGCGLP GGESGFNIGR VVAVALGYDF LPGTTVNRYC SSSLQITRVA FHAIKAGEGD AFISAGVETV SRFGKGNSDS LPDTKNPLFD EAQERTAAAA AGAEEWHDPR ADGNLPDVYI AMGQTAENVA IISGISREDQ DHWGVRSQNR AEEAIKGGFF EREISPVTLP DGTTVSTNDG PRAGTTYEKV SQLKPVFRPN GTVTAGNACP LNDGAAAVVI TSDTKAKELG LTPLARIVST GVSGLSPEIM GLGPIEACKK ALQRAGMSIS DIDLVEINEA FAVQVLGSAR ELGIDEDELN VSGGAIALGH PFGMTGARIT TTLLNNLQTH DKTFGLETMC VGGGQGMAMV IERLS
NT Sequence
ATGCCAGAAG CCGTCATCGT CTCAACAGCC CGCTCGCCGA TCGGCCGCGC CATGAAAGCG TCGCTGGTCA GCATGCGCCC CGACGACTTG GCCGTGCAGA TGGTGCGCGC CGCGTTGGAC AAAGTACCCG CGCTGAACCC GCACCAGATC GACGATCTGA TCATGGGCTG CGGCCTCCCG GGAGGTGAGT CCGGCTTCAA TATCGGGCGC GTCGTCGCCG TGGCGCTGGG CTACGACTTT CTGCCGGGGA CCACGGTCAA CCGGTACTGC TCGTCTTCAC TGCAGATCAC CCGGGTGGCG TTTCATGCGA TCAAGGCCGG CGAGGGCGAC GCGTTCATCT CCGCGGGCGT AGAGACCGTG TCCCGGTTCG GCAAGGGCAA CTCCGACTCG CTGCCCGACA CCAAGAACCC CCTCTTCGAT GAGGCCCAGG AACGCACCGC CGCCGCGGCC GCGGGTGCCG AGGAATGGCA TGACCCGCGC GCTGACGGAA ATCTGCCTGA CGTCTACATC GCCATGGGTC AGACCGCTGA AAACGTCGCG ATCATTTCGG GCATCAGCCG CGAAGACCAA GACCACTGGG GTGTGCGCAG CCAAAACCGT GCCGAAGAGG CGATCAAGGG CGGCTTTTTC GAACGGGAGA TCTCCCCGGT GACGCTTCCC GACGGCACCA CGGTCAGCAC CAATGATGGC CCGCGCGCGG GTACCACCTA CGAAAAGGTC AGCCAACTCA AGCCGGTGTT TCGGCCCAAC GGCACAGTGA CCGCCGGCAA CGCCTGTCCA CTCAACGACG GCGCCGCGGC AGTGGTGATC ACCAGCGACA CCAAGGCCAA GGAACTGGGC CTGACGCCGT TGGCGCGCAT CGTGTCCACC GGCGTGAGCG GTCTGTCCCC GGAGATCATG GGCCTTGGCC CCATCGAGGC TTGCAAGAAG GCGCTACAGC GGGCGGGTAT GTCCATCAGC GACATCGACT TGGTCGAGAT CAATGAAGCG TTCGCGGTGC AGGTGCTGGG CTCGGCGCGC GAGTTGGGCA TCGACGAGGA CGAGCTGAAC GTCTCTGGCG GAGCGATCGC CCTGGGACAC CCGTTCGGCA TGACCGGCGC CCGCATCACC ACCACCTTGC TCAACAATCT GCAGACCCAC GACAAGACGT TCGGCCTGGA GACCATGTGC GTCGGTGGCG GCCAGGGCAT GGCCATGGTG ATCGAGCGGC TGAGC
Details for MyulA.00781.a.A1.GE29738
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 74
PERCENT COVERAGE: 70
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVST ARSPIGRAMK ASLVSMRPDD LAVQMVRAAL DKVPALNPHQ IDDLIMGCGL PGGESGFNIG RVVAVALGYD FLPGTTVNRY CSSSLQITRV AFHAIKAGEG DAFISAGVET VSRFGKGNSD SLPDTKNPLF DEAQERTAAA AAGAEEWHDP RADGNLPDVY IAMGQTAENV AIISGISRED QDHWGVRXXT XPXRRSXAXF XTXXXPGXAX XXXXVXXXXX XXXXXKXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
ttttgtttan ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccag aagccgtcat cgtctcaaca gcccgctcgc cgatcggccg cgccatgaaa gcgtcgctgg tcagcatgcg ccccgacgac ttggccgtgc agatggtgcg cgccgcgttg gacaaagtac ccgcgctgaa cccgcaccag atcgacgatc tgatcatggg ctgcggcctc ccgggaggtg agtccggctt caatatcggg cgcgtcgtcg ccgtggcgct gggctacgac tttctgccgg ggaccacggt caaccggtac tgctcgtctt cactgcagat cacccgggtg gcgtttcatg cgatcaaggc cggcgagggc gacgcgttca tctccgcggg cgtagagacc gtgtcccggt tcggcaaggg caactccgac tcgctgcccg acaccaagaa ccccctcttc gatgaggccc aggaacgcac cgccgccgcg gccgcgggtg ccgaggaatg gcatgacccg cgcgctgacg gaaatctgcc tgacgtctac atcgccatgg gtcagaccgc tgaaaacgtc gcgatcattt cgggcatcag ccgcgaagac caagaccact ggggtgtgcg cannnaaacc gngccgaana ggcgatcang ggcggntttt cnaacggana nnnnnccngg ngangcttnc nnangnnccn nngtcagnan nnngnnnnnn ngnnggnncn nnncnaaann gncannnant nannnnnnnt tnnnnnnnnn ncnnncnnnn ncnnnnnnnn tcnnnnnnnn nnnnnannnn nnnnngntnn nnnnnnnnnn nnnnnnnnng nnnnnnnnnn nngnnnnnnn nnnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPEAVIVST ARSPIGRAMK ASLVSMRPDD LAVQMVRAAL DKVPALNPHQ IDDLIMGCGL PGGESGFNIG RVVAVALGYD FLPGTTVNRY CSSSLQITRV AFHAIKAGEG DAFISAGVET VSRFGKGNSD SLPDTKNPLF DEAQERTAAA AAGAEEWHDP RADGNLPDVY IAMGQTAENV AIISGISRED QDHWGVRSQN RAEEAIKGGF FEREISPVTL PDGTTVSTND GPRAGTTYEK VSQLKPVFRP NGTVTAGNAC PLNDGAAAVV ITSDTKAKEL GLTPLARIVS TGVSGLSPEI MGLGPIEACK KALQRAGMSI SDIDLVEINE AFAVQVLGSA RELGIDEDEL NVSGGAIALG HPFGMTGARI TTTLLNNLQT HDKTFGLETM CVGGGQGMAM VIERLS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccagaagcc gtcatcgtct caacagcccg ctcgccgatc ggccgcgcca tgaaagcgtc gctggtcagc atgcgccccg acgacttggc cgtgcagatg gtgcgcgccg cgttggacaa agtacccgcg ctgaacccgc accagatcga cgatctgatc atgggctgcg gcctcccggg aggtgagtcc ggcttcaata tcgggcgcgt cgtcgccgtg gcgctgggct acgactttct gccggggacc acggtcaacc ggtactgctc gtcttcactg cagatcaccc gggtggcgtt tcatgcgatc aaggccggcg agggcgacgc gttcatctcc gcgggcgtag agaccgtgtc ccggttcggc aagggcaact ccgactcgct gcccgacacc aagaaccccc tcttcgatga ggcccaggaa cgcaccgccg ccgcggccgc gggtgccgag gaatggcatg acccgcgcgc tgacggaaat ctgcctgacg tctacatcgc catgggtcag accgctgaaa acgtcgcgat catttcgggc atcagccgcg aagaccaaga ccactggggt gtgcgcagcc aaaaccgtgc cgaagaggcg atcaagggcg gctttttcga acgggagatc tccccggtga cgcttcccga cggcaccacg gtcagcacca atgatggccc gcgcgcgggt accacctacg aaaaggtcag ccaactcaag ccggtgtttc ggcccaacgg cacagtgacc gccggcaacg cctgtccact caacgacggc gccgcggcag tggtgatcac cagcgacacc aaggccaagg aactgggcct gacgccgttg gcgcgcatcg tgtccaccgg cgtgagcggt ctgtccccgg agatcatggg ccttggcccc atcgaggctt gcaagaaggc gctacagcgg gcgggtatgt ccatcagcga catcgacttg gtcgagatca atgaagcgtt cgcggtgcag gtgctgggct cggcgcgcga gttgggcatc gacgaggacg agctgaacgt ctctggcgga gcgatcgccc tgggacaccc gttcggcatg accggcgccc gcatcaccac caccttgctc aacaatctgc agacccacga caagacgttc ggcctggaga ccatgtgcgt cggtggcggc cagggcatgg ccatggtgat cgagcggctg agcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa